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The map label for this gene is btuB [H]

Identifier: 194334113

GI number: 194334113

Start: 1420324

End: 1422228

Strand: Reverse

Name: btuB [H]

Synonym: Paes_1301

Alternate gene names: 194334113

Gene position: 1422228-1420324 (Counterclockwise)

Preceding gene: 194334114

Following gene: 194334112

Centisome position: 56.6

GC content: 52.34

Gene sequence:

>1905_bases
ATGCAAAAAAAAATTATCGCTCTTCTCATCGCGAGTGTGTGTTGTCACTGCACGCTTTATGCTGACGATTCATCTCAATC
GGCAATTCTTTCGTCCTACACCGGTGACGAGCTTGTGGTGACCGCGACACGCTTTCCCATCAAGGAAAAGGAGAGTTCTC
GCTTCATTACTGTCGCTGACAGCGAAAAGCTCAAGGAAGTCGGTGCGACAAACGCCATGGATGCCCTCGGAAAAATCGGA
GGGATCGGCTACAAGTCTCTCGCCCCGATGGGGATCAACAGGCAGGGAATGAATGCCGCAGTCTACATCAGGGGGATCGA
GGACGGGGAGCTGATCCTGATCAACGGAATGCCTGTCCAGCAGGCTTCGGGCAAAGGATATGACATCAGTTCCATTCCTG
TCGAGCAGATCGAGCGGATCGAGGTACTCAAAGGCGCAGCATCGACCCTTTACGGGGCGGATGCGATGGCCGGCGTGATC
AATATCGTGATGAAAAAGGCCGTCGATGAAAAAAGCGGAACCGCATCTGTTGAGTTCGGAAACGAAGAGTGGATGAATCA
CGGTGTCAGTTTCTCTCTTCCCGGCATTTCCGCCGGTGTTCGTTACCAGCATATGGGCGAACTGGATGATGTTGGCAGGG
ATCTGAAAAACGAGCACACGATGGCGCTCGGCAACACTGACAAGTATATGGTCAATGTCAACGCATCACCCTTCGACAAC
GTCTCTGTCGACTACCAGTATAACTGGTACGAAACGACTTTCATCGACCGGTACGATTCAGGCGAAGAGGAGTCGACCGA
TCAGGAAAGCAGTTTCCACTTCCTGAGCCTTCGCTATGAGACTGCAAGGTTCAAGGCCAAGGCGTTCGGTGTTTACGACA
ACCGGTACCAGACGGACTATGTCAATGAAGAACAGGATGGAGAGACCTTGCAGAGGCTGAATTATAACTATGGGGCGGAA
ACGGATTATCGCTTCAAGTTCTCTGAGGCTTTCGAGCTGGTCGCCGGAGCCGATTATGTGCATCGTTTTGCTGATTACGA
GAACAATTACGGTGAGAAATACCGTGACGATTACGGGCTTTTTGCCGAGCTCAAAGCAGAGCCGTCATCTGATTTGCTTC
TTGCCCTTGGCGTTCGTGAGCAGTTCATCGATAACGAGGACGGTACGTCGGACTACGATGCATTTCTTCCCAGCGCAGGT
GTGACATGGAAAGTCAATGAAGAGCTGAACCTCTTTGCCAATACAGGCAAGGCGTTCCAGGCTCCGACCTTTACCCAGCT
GTATTACGAGGGCAGGATCATTGTTGGCAACCCTGATCTCGAGCCGGAGTCCGGCTGGACCTATGAAGCTGGATTCAAGT
ACGATTCCGGGGTGGCATCTGCAAGGCTGGCCGGTTTTTACATGACCTATGATGACAAGATCGAAGTCGACCGTTCACAG
GGGCGTCCATACGGTTATTTCAATGCAGGATCATACAGTTCCAAAGGGGTCGAGTGGAAACTCGGGCTGTATCCTTTCCT
CTCTTCCGGCAGCTTTCTTTCCGATGTTTCCTTCTCGGCTGCCGGTTACTGGGCGTACCCGGTTGCCGAGAACACTGACG
GAGAGGAGTATCAGCCCGGTCCGAAGTTCCAGAACACGTTCGGAATCTCCTATGCGACCCTGCCGGTCAATGTCGACCTT
ACCTGCCGCATTCTTGCCGGCCGCGAGGACGAGCTTGAAAATTATGCGGCTTTTGATATGACAGGAAAGGTGAAGGCCGG
TTCAGGTTACGTGACGCTTGCTGTGGAGAATGTCTTCGATACCGAGATACAGACGACCGGAAATCTTGTTGAAGATGCCA
GCAGCCGCTACGTGTATTACGAGCCCGGGCGACTTGCCAGGCTTGGTTATACGGTGTCTTTTTGA

Upstream 100 bases:

>100_bases
ATGTCCGACGACGCATAGGCTATTGCTATCACGTTTCTTCTGTGCCATCAGGACAGCCCTGTCAGTCAGTGTTTTATTAA
CTGATCAACAGGATCTACGA

Downstream 100 bases:

>100_bases
GTTGAAGAGTTGTGATGTATCGATCTTCCATGATGTGTTCATCATGGGAGGTCGGTGCGGTTCTATCGGATGGATTTCCG
GGAAAAGTATGAACGAAAAT

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MQKKIIALLIASVCCHCTLYADDSSQSAILSSYTGDELVVTATRFPIKEKESSRFITVADSEKLKEVGATNAMDALGKIG
GIGYKSLAPMGINRQGMNAAVYIRGIEDGELILINGMPVQQASGKGYDISSIPVEQIERIEVLKGAASTLYGADAMAGVI
NIVMKKAVDEKSGTASVEFGNEEWMNHGVSFSLPGISAGVRYQHMGELDDVGRDLKNEHTMALGNTDKYMVNVNASPFDN
VSVDYQYNWYETTFIDRYDSGEEESTDQESSFHFLSLRYETARFKAKAFGVYDNRYQTDYVNEEQDGETLQRLNYNYGAE
TDYRFKFSEAFELVAGADYVHRFADYENNYGEKYRDDYGLFAELKAEPSSDLLLALGVREQFIDNEDGTSDYDAFLPSAG
VTWKVNEELNLFANTGKAFQAPTFTQLYYEGRIIVGNPDLEPESGWTYEAGFKYDSGVASARLAGFYMTYDDKIEVDRSQ
GRPYGYFNAGSYSSKGVEWKLGLYPFLSSGSFLSDVSFSAAGYWAYPVAENTDGEEYQPGPKFQNTFGISYATLPVNVDL
TCRILAGREDELENYAAFDMTGKVKAGSGYVTLAVENVFDTEIQTTGNLVEDASSRYVYYEPGRLARLGYTVSF

Sequences:

>Translated_634_residues
MQKKIIALLIASVCCHCTLYADDSSQSAILSSYTGDELVVTATRFPIKEKESSRFITVADSEKLKEVGATNAMDALGKIG
GIGYKSLAPMGINRQGMNAAVYIRGIEDGELILINGMPVQQASGKGYDISSIPVEQIERIEVLKGAASTLYGADAMAGVI
NIVMKKAVDEKSGTASVEFGNEEWMNHGVSFSLPGISAGVRYQHMGELDDVGRDLKNEHTMALGNTDKYMVNVNASPFDN
VSVDYQYNWYETTFIDRYDSGEEESTDQESSFHFLSLRYETARFKAKAFGVYDNRYQTDYVNEEQDGETLQRLNYNYGAE
TDYRFKFSEAFELVAGADYVHRFADYENNYGEKYRDDYGLFAELKAEPSSDLLLALGVREQFIDNEDGTSDYDAFLPSAG
VTWKVNEELNLFANTGKAFQAPTFTQLYYEGRIIVGNPDLEPESGWTYEAGFKYDSGVASARLAGFYMTYDDKIEVDRSQ
GRPYGYFNAGSYSSKGVEWKLGLYPFLSSGSFLSDVSFSAAGYWAYPVAENTDGEEYQPGPKFQNTFGISYATLPVNVDL
TCRILAGREDELENYAAFDMTGKVKAGSGYVTLAVENVFDTEIQTTGNLVEDASSRYVYYEPGRLARLGYTVSF
>Mature_634_residues
MQKKIIALLIASVCCHCTLYADDSSQSAILSSYTGDELVVTATRFPIKEKESSRFITVADSEKLKEVGATNAMDALGKIG
GIGYKSLAPMGINRQGMNAAVYIRGIEDGELILINGMPVQQASGKGYDISSIPVEQIERIEVLKGAASTLYGADAMAGVI
NIVMKKAVDEKSGTASVEFGNEEWMNHGVSFSLPGISAGVRYQHMGELDDVGRDLKNEHTMALGNTDKYMVNVNASPFDN
VSVDYQYNWYETTFIDRYDSGEEESTDQESSFHFLSLRYETARFKAKAFGVYDNRYQTDYVNEEQDGETLQRLNYNYGAE
TDYRFKFSEAFELVAGADYVHRFADYENNYGEKYRDDYGLFAELKAEPSSDLLLALGVREQFIDNEDGTSDYDAFLPSAG
VTWKVNEELNLFANTGKAFQAPTFTQLYYEGRIIVGNPDLEPESGWTYEAGFKYDSGVASARLAGFYMTYDDKIEVDRSQ
GRPYGYFNAGSYSSKGVEWKLGLYPFLSSGSFLSDVSFSAAGYWAYPVAENTDGEEYQPGPKFQNTFGISYATLPVNVDL
TCRILAGREDELENYAAFDMTGKVKAGSGYVTLAVENVFDTEIQTTGNLVEDASSRYVYYEPGRLARLGYTVSF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4771

COG function: function code P; Outer membrane receptor for ferrienterochelin and colicins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=511, Percent_Identity=24.26614481409, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1790405, Length=189, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787024, Length=181, Percent_Identity=31.4917127071823, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1786798, Length=211, Percent_Identity=29.8578199052133, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 70404; Mature: 70404

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKIIALLIASVCCHCTLYADDSSQSAILSSYTGDELVVTATRFPIKEKESSRFITVAD
CCHHHHHHHHHHHHHEEEEEECCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCEEEEEC
SEKLKEVGATNAMDALGKIGGIGYKSLAPMGINRQGMNAAVYIRGIEDGELILINGMPVQ
CHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCC
QASGKGYDISSIPVEQIERIEVLKGAASTLYGADAMAGVINIVMKKAVDEKSGTASVEFG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEEEC
NEEWMNHGVSFSLPGISAGVRYQHMGELDDVGRDLKNEHTMALGNTDKYMVNVNASPFDN
CHHHHHCCCEEECCCCCCCCCCHHCCCHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCC
VSVDYQYNWYETTFIDRYDSGEEESTDQESSFHFLSLRYETARFKAKAFGVYDNRYQTDY
EEEEEEECEEEEEEEECCCCCCCCCCCCCCCEEEEEEEEEHHHEEEEEEEEECCCEECCC
VNEEQDGETLQRLNYNYGAETDYRFKFSEAFELVAGADYVHRFADYENNYGEKYRDDYGL
CCCCCCCHHHHHCCCCCCCCCCEEEEHHHHHHHHHCHHHHHHHHHCCCCCCHHHCCCCCE
FAELKAEPSSDLLLALGVREQFIDNEDGTSDYDAFLPSAGVTWKVNEELNLFANTGKAFQ
EEEEECCCCCCEEEEECCHHHHCCCCCCCCCHHHHCCCCCCEEEECCCEEEEECCCCCCC
APTFTQLYYEGRIIVGNPDLEPESGWTYEAGFKYDSGVASARLAGFYMTYDDKIEVDRSQ
CCCEEEEEEECEEEECCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEEEECCEEEEECCC
GRPYGYFNAGSYSSKGVEWKLGLYPFLSSGSFLSDVSFSAAGYWAYPVAENTDGEEYQPG
CCCCEEECCCCCCCCCEEEEEECEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
PKFQNTFGISYATLPVNVDLTCRILAGREDELENYAAFDMTGKVKAGSGYVTLAVENVFD
CCCCCEECCEEEEEEEECCEEEEEECCCCHHHCCEEEECCCCEEECCCCEEEEEEECCCC
TEIQTTGNLVEDASSRYVYYEPGRLARLGYTVSF
CCHHCCCCHHHCCCCCEEEECCCCEEEEEEEEEC
>Mature Secondary Structure
MQKKIIALLIASVCCHCTLYADDSSQSAILSSYTGDELVVTATRFPIKEKESSRFITVAD
CCHHHHHHHHHHHHHEEEEEECCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCEEEEEC
SEKLKEVGATNAMDALGKIGGIGYKSLAPMGINRQGMNAAVYIRGIEDGELILINGMPVQ
CHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCC
QASGKGYDISSIPVEQIERIEVLKGAASTLYGADAMAGVINIVMKKAVDEKSGTASVEFG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEEEC
NEEWMNHGVSFSLPGISAGVRYQHMGELDDVGRDLKNEHTMALGNTDKYMVNVNASPFDN
CHHHHHCCCEEECCCCCCCCCCHHCCCHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCC
VSVDYQYNWYETTFIDRYDSGEEESTDQESSFHFLSLRYETARFKAKAFGVYDNRYQTDY
EEEEEEECEEEEEEEECCCCCCCCCCCCCCCEEEEEEEEEHHHEEEEEEEEECCCEECCC
VNEEQDGETLQRLNYNYGAETDYRFKFSEAFELVAGADYVHRFADYENNYGEKYRDDYGL
CCCCCCCHHHHHCCCCCCCCCCEEEEHHHHHHHHHCHHHHHHHHHCCCCCCHHHCCCCCE
FAELKAEPSSDLLLALGVREQFIDNEDGTSDYDAFLPSAGVTWKVNEELNLFANTGKAFQ
EEEEECCCCCCEEEEECCHHHHCCCCCCCCCHHHHCCCCCCEEEECCCEEEEECCCCCCC
APTFTQLYYEGRIIVGNPDLEPESGWTYEAGFKYDSGVASARLAGFYMTYDDKIEVDRSQ
CCCEEEEEEECEEEECCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEEEECCEEEEECCC
GRPYGYFNAGSYSSKGVEWKLGLYPFLSSGSFLSDVSFSAAGYWAYPVAENTDGEEYQPG
CCCCEEECCCCCCCCCEEEEEECEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
PKFQNTFGISYATLPVNVDLTCRILAGREDELENYAAFDMTGKVKAGSGYVTLAVENVFD
CCCCCEECCEEEEEEEECCEEEEEECCCCHHHCCEEEECCCCEEECCCCEEEEEEECCCC
TEIQTTGNLVEDASSRYVYYEPGRLARLGYTVSF
CCHHCCCCHHHCCCCCEEEECCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA