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The map label for this gene is btuB [H]

Identifier: 194334086

GI number: 194334086

Start: 1380335

End: 1382920

Strand: Reverse

Name: btuB [H]

Synonym: Paes_1274

Alternate gene names: 194334086

Gene position: 1382920-1380335 (Counterclockwise)

Preceding gene: 194334087

Following gene: 194334085

Centisome position: 55.03

GC content: 51.08

Gene sequence:

>2586_bases
ATGCGCAATTATAGAGTTGTTCTCTGTTGGAGAGCAGCGGTCGGGTGCGTCGTGCGTCGTGTTCGACCTTCGGTCGGCTG
CTGTCTGGAAAGGGTGTTGTCGTCGCCAGCAGGAAAAGAACGACCGGGCATTTCCTGGCCCCAGGGAGCTTGCCGTGCCC
GGGACGGTCCGTTCTCTGGTGCCCAGGCATGGTTCCGGCCATCCGGTTCCGGAAATAACACACTGTTGTACACGCAGTAC
ATAAACCTAACTATTGTCATTATGAGAAGTTTTTTTGTGGGGAGATCGATAGCTGTGGCGCTTCTGATCTCCATGTTTTC
CTTTTCGTTTACAGCTGGTGCTCTGGCGGAAAACAGGTCGATTTCCGGGATCGTATCCGGAGCATCTGATGGAGAACCGC
TTGTCGGTGCTTCGGTGCAGGTCAAGGGTACAGGTATCGGCACCGTTACGGATGTGCAGGGTGAATACCGGCTGGACATC
CCGGAGGATGCCGATGCTGTAACATTCAGTTCTATCGGCTACGAGCCCAGAACGGTTGCTCCCGGTACGGGAAGCATCCT
GAATGTGAGCCTGAACGGGCACAGCTATTCGACAGGTGAGATTGTGGTGTATTCCACTCGTTCCATGGCGAAACTGAAGA
ATATTCCCCGAAAGGTGGAGATTGTCAGTGAAAAAGATATCGAGGCTCTTGACCCTGTCAATGCGACCGACCTGCTGAAA
AAAACTTCGGGGGTCGATATTATCGAGTATCCCGGCGTTCTCTCTGGAGTGTCGATCCGGGGTTTTTCACCGGATTTTTC
AGGGATCGATCAGAATGTTACCTATCTGATCGACGGCCGGCCGGCGGGGGCGACCAATCTTGCAACGATCGACATGAACA
ATATCGAGCGTGTCGAAGTGATCAAGGGACCCTCCTCTGCGCTGTATGGCTCTCAGGGTATGGGAGGCAGCATCAATTTC
ATTACCCGGAAATCAGCCGGAGCGCTTTCCGGTAAGGCTTCTCTCGGCTACAGCAGTTTCAGTACTCTTGATGGTTCGGT
GCGTGTTGGCGGTTCGCTGTCAGAGCGTTTTGATGTCGATCTCGGGGTCAGGAGCTATAATCAGAACGAGGATTATACGG
TCGGTTCCAATACCCTGATTTCCGATCCTGATCCGGAAGTGCTCGAACAGGATATCGATACGATGAGGAACTCAACCTAT
GCCACGCAATCAGGCAATCTGCGTATCGGCTACCAGCTCAATGATGAGGTACGGATCGATGCCCGGGGAGAGATCTACCA
GGCTCACAGCGTCAAAAGCCCTGGGTCGATCTGGGGCGTCTACGGTCACAGCGAAAAAGATATCGACCGCCAGACTTTGG
ACGTTACGATGACGGCGGACTGGGGGACTCATGCCCTGCGTTTCGTCCCGCACTGGTCACTGGAAAAATCCGTGAGTTAC
AAAAAAGATGATGAAGGGCTCAATTACCGTTCGTCTCTCAGTGAAATCGAGTGGCTTGGATTTCAGCTTCAGGACCGGAT
ACGTGTTGGCCGGCAGACGTTTACCGGAGGGGTTGATTATACGGTGATCGACGTGTTTTCAAGAAGTTTTGCGGATTCAG
AGACTGAAAAAGCACCCTCTCGCCCCAATCAGCAGCAGTCATCTCTGGGAGTGTTCGGTGAGGTTGGAATTTCGTTGTTT
GACGAGACGGTGATTGCCAATATCGGCGGGCGTTATGATATCACGTCGTACCAGTTGAAGGAAACACCTTTGTTTACAAC
TGTCGAGACGGAATCGGAAGAGTATGACAACTTTAATCCCAGTGTCAGCGTCCAGTACCGGTTTTTGCCGCAGTGCAAAA
TTCACGCAAGTATAGGAAGGGCTTTCGTTGCACCGAATCCTTATGAGAAAGCGGGATTTTATGTTGATGACGATGGTATG
GAAACCCGGGGCAATCCCGACCTCGAGCCGGAAACAAGCGTGACCTGGGATGCCGGACTGACCTATGAATCCGATAATGG
CGGATTCCGGACAGATGTCACCTGCTTCAGTACCGACTGGGAGAACTTTATTCAGAGAACAAGCGCTGTCGATGAATTCG
GAGAGGAGTATAAGACATTCGAGAATCTCAATTCGGCAGAGATGAATGGCATTGAACTCGAATGTTCCTATGATTTTGGT
GCTGCACACGATGAGCGGTATTCGCTGCGCCTTTTTACAAATTTTACGCACCTGTTCAGAGCCAGATCTATCAATGACGA
TGGTTCAGAAGGGGATATGCTCTATGTCAGACGCAACAGGGGCAGTATGGGATTGGAGTATGATGATTTCAGGTTTTTTT
CTGCGCGTCTGACGGCAAGGTATATCGGTGACCGGTTCGAGCAGAACTGGATCGGGCGCTATGACGAGGCACGACCGACC
TTGAAGGACCCTGTCATTGAAATTCCGGCGAGCCTTATTTTTGATATGTCGATGGGTTTCAGAATCGATGATCAGAATGA
TGTGAGCCTGATAGCGAAAAACATTCTTGATGAGAACTACACCGAAAAAGACGGCTACAATATGCCAGGAAGATCCCTTG
GTGTTCGATATTCGGTAGAATTTTAG

Upstream 100 bases:

>100_bases
ACTGTCGCGCACCCCGCGCAACGGGAAGGCCTGCAGACCGGGGGAGAGTCAGAAGACCTGCCGTAATGGAGAATGCTTCG
GGAAGAAAGCGAGTCTTGTA

Downstream 100 bases:

>100_bases
TGATATGATGGTTGTGTTTTTTCGCTTGTTTGTCAAGCGGGGACGGACAGTGCTGATACCAGCGCTGTCCATGGCCCTCA
TGTTGACCGGTCGTTCGAGA

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 861; Mature: 861

Protein sequence:

>861_residues
MRNYRVVLCWRAAVGCVVRRVRPSVGCCLERVLSSPAGKERPGISWPQGACRARDGPFSGAQAWFRPSGSGNNTLLYTQY
INLTIVIMRSFFVGRSIAVALLISMFSFSFTAGALAENRSISGIVSGASDGEPLVGASVQVKGTGIGTVTDVQGEYRLDI
PEDADAVTFSSIGYEPRTVAPGTGSILNVSLNGHSYSTGEIVVYSTRSMAKLKNIPRKVEIVSEKDIEALDPVNATDLLK
KTSGVDIIEYPGVLSGVSIRGFSPDFSGIDQNVTYLIDGRPAGATNLATIDMNNIERVEVIKGPSSALYGSQGMGGSINF
ITRKSAGALSGKASLGYSSFSTLDGSVRVGGSLSERFDVDLGVRSYNQNEDYTVGSNTLISDPDPEVLEQDIDTMRNSTY
ATQSGNLRIGYQLNDEVRIDARGEIYQAHSVKSPGSIWGVYGHSEKDIDRQTLDVTMTADWGTHALRFVPHWSLEKSVSY
KKDDEGLNYRSSLSEIEWLGFQLQDRIRVGRQTFTGGVDYTVIDVFSRSFADSETEKAPSRPNQQQSSLGVFGEVGISLF
DETVIANIGGRYDITSYQLKETPLFTTVETESEEYDNFNPSVSVQYRFLPQCKIHASIGRAFVAPNPYEKAGFYVDDDGM
ETRGNPDLEPETSVTWDAGLTYESDNGGFRTDVTCFSTDWENFIQRTSAVDEFGEEYKTFENLNSAEMNGIELECSYDFG
AAHDERYSLRLFTNFTHLFRARSINDDGSEGDMLYVRRNRGSMGLEYDDFRFFSARLTARYIGDRFEQNWIGRYDEARPT
LKDPVIEIPASLIFDMSMGFRIDDQNDVSLIAKNILDENYTEKDGYNMPGRSLGVRYSVEF

Sequences:

>Translated_861_residues
MRNYRVVLCWRAAVGCVVRRVRPSVGCCLERVLSSPAGKERPGISWPQGACRARDGPFSGAQAWFRPSGSGNNTLLYTQY
INLTIVIMRSFFVGRSIAVALLISMFSFSFTAGALAENRSISGIVSGASDGEPLVGASVQVKGTGIGTVTDVQGEYRLDI
PEDADAVTFSSIGYEPRTVAPGTGSILNVSLNGHSYSTGEIVVYSTRSMAKLKNIPRKVEIVSEKDIEALDPVNATDLLK
KTSGVDIIEYPGVLSGVSIRGFSPDFSGIDQNVTYLIDGRPAGATNLATIDMNNIERVEVIKGPSSALYGSQGMGGSINF
ITRKSAGALSGKASLGYSSFSTLDGSVRVGGSLSERFDVDLGVRSYNQNEDYTVGSNTLISDPDPEVLEQDIDTMRNSTY
ATQSGNLRIGYQLNDEVRIDARGEIYQAHSVKSPGSIWGVYGHSEKDIDRQTLDVTMTADWGTHALRFVPHWSLEKSVSY
KKDDEGLNYRSSLSEIEWLGFQLQDRIRVGRQTFTGGVDYTVIDVFSRSFADSETEKAPSRPNQQQSSLGVFGEVGISLF
DETVIANIGGRYDITSYQLKETPLFTTVETESEEYDNFNPSVSVQYRFLPQCKIHASIGRAFVAPNPYEKAGFYVDDDGM
ETRGNPDLEPETSVTWDAGLTYESDNGGFRTDVTCFSTDWENFIQRTSAVDEFGEEYKTFENLNSAEMNGIELECSYDFG
AAHDERYSLRLFTNFTHLFRARSINDDGSEGDMLYVRRNRGSMGLEYDDFRFFSARLTARYIGDRFEQNWIGRYDEARPT
LKDPVIEIPASLIFDMSMGFRIDDQNDVSLIAKNILDENYTEKDGYNMPGRSLGVRYSVEF
>Mature_861_residues
MRNYRVVLCWRAAVGCVVRRVRPSVGCCLERVLSSPAGKERPGISWPQGACRARDGPFSGAQAWFRPSGSGNNTLLYTQY
INLTIVIMRSFFVGRSIAVALLISMFSFSFTAGALAENRSISGIVSGASDGEPLVGASVQVKGTGIGTVTDVQGEYRLDI
PEDADAVTFSSIGYEPRTVAPGTGSILNVSLNGHSYSTGEIVVYSTRSMAKLKNIPRKVEIVSEKDIEALDPVNATDLLK
KTSGVDIIEYPGVLSGVSIRGFSPDFSGIDQNVTYLIDGRPAGATNLATIDMNNIERVEVIKGPSSALYGSQGMGGSINF
ITRKSAGALSGKASLGYSSFSTLDGSVRVGGSLSERFDVDLGVRSYNQNEDYTVGSNTLISDPDPEVLEQDIDTMRNSTY
ATQSGNLRIGYQLNDEVRIDARGEIYQAHSVKSPGSIWGVYGHSEKDIDRQTLDVTMTADWGTHALRFVPHWSLEKSVSY
KKDDEGLNYRSSLSEIEWLGFQLQDRIRVGRQTFTGGVDYTVIDVFSRSFADSETEKAPSRPNQQQSSLGVFGEVGISLF
DETVIANIGGRYDITSYQLKETPLFTTVETESEEYDNFNPSVSVQYRFLPQCKIHASIGRAFVAPNPYEKAGFYVDDDGM
ETRGNPDLEPETSVTWDAGLTYESDNGGFRTDVTCFSTDWENFIQRTSAVDEFGEEYKTFENLNSAEMNGIELECSYDFG
AAHDERYSLRLFTNFTHLFRARSINDDGSEGDMLYVRRNRGSMGLEYDDFRFFSARLTARYIGDRFEQNWIGRYDEARPT
LKDPVIEIPASLIFDMSMGFRIDDQNDVSLIAKNILDENYTEKDGYNMPGRSLGVRYSVEF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=676, Percent_Identity=21.4497041420118, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788478, Length=147, Percent_Identity=31.9727891156463, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 95174; Mature: 95174

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNYRVVLCWRAAVGCVVRRVRPSVGCCLERVLSSPAGKERPGISWPQGACRARDGPFSG
CCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQAWFRPSGSGNNTLLYTQYINLTIVIMRSFFVGRSIAVALLISMFSFSFTAGALAENRS
CCCEECCCCCCCCEEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
ISGIVSGASDGEPLVGASVQVKGTGIGTVTDVQGEYRLDIPEDADAVTFSSIGYEPRTVA
CEEEEECCCCCCCEECCEEEEECCCCCEEEECCCCEEECCCCCCCCEEEHHCCCCCCEEC
PGTGSILNVSLNGHSYSTGEIVVYSTRSMAKLKNIPRKVEIVSEKDIEALDPVNATDLLK
CCCCCEEEEEECCCCCCCCCEEEEECHHHHHHHCCCCEEEEECCCCCHHCCCCCHHHHHH
KTSGVDIIEYPGVLSGVSIRGFSPDFSGIDQNVTYLIDGRPAGATNLATIDMNNIERVEV
HCCCCEEEECCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEE
IKGPSSALYGSQGMGGSINFITRKSAGALSGKASLGYSSFSTLDGSVRVGGSLSERFDVD
EECCCHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCEEEE
LGVRSYNQNEDYTVGSNTLISDPDPEVLEQDIDTMRNSTYATQSGNLRIGYQLNDEVRID
ECEECCCCCCCEECCCCCEECCCCHHHHHHHHHHHHCCCEEECCCCEEEEEEECCEEEEC
ARGEIYQAHSVKSPGSIWGVYGHSEKDIDRQTLDVTMTADWGTHALRFVPHWSLEKSVSY
CCCCEEEECCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCC
KKDDEGLNYRSSLSEIEWLGFQLQDRIRVGRQTFTGGVDYTVIDVFSRSFADSETEKAPS
CCCCCCCCHHHHHHHHHHHCHHHHHHHHHCHHHCCCCCCEEHHHHHHHHHCCCHHHCCCC
RPNQQQSSLGVFGEVGISLFDETVIANIGGRYDITSYQLKETPLFTTVETESEEYDNFNP
CCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCEEEEECCCEEEEEECCCHHCCCCCC
SVSVQYRFLPQCKIHASIGRAFVAPNPYEKAGFYVDDDGMETRGNPDLEPETSVTWDAGL
CEEEEEEECCCEEEEHHHCEEEECCCCHHHCCEEECCCCCCCCCCCCCCCCCCEEECCCE
TYESDNGGFRTDVTCFSTDWENFIQRTSAVDEFGEEYKTFENLNSAEMNGIELECSYDFG
EEECCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
AAHDERYSLRLFTNFTHLFRARSINDDGSEGDMLYVRRNRGSMGLEYDDFRFFSARLTAR
CCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEECCHHEEHHHHHHH
YIGDRFEQNWIGRYDEARPTLKDPVIEIPASLIFDMSMGFRIDDQNDVSLIAKNILDENY
HHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHEEECCCCEEECCCCCHHHHHHHHHCCCC
TEKDGYNMPGRSLGVRYSVEF
CCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MRNYRVVLCWRAAVGCVVRRVRPSVGCCLERVLSSPAGKERPGISWPQGACRARDGPFSG
CCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQAWFRPSGSGNNTLLYTQYINLTIVIMRSFFVGRSIAVALLISMFSFSFTAGALAENRS
CCCEECCCCCCCCEEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
ISGIVSGASDGEPLVGASVQVKGTGIGTVTDVQGEYRLDIPEDADAVTFSSIGYEPRTVA
CEEEEECCCCCCCEECCEEEEECCCCCEEEECCCCEEECCCCCCCCEEEHHCCCCCCEEC
PGTGSILNVSLNGHSYSTGEIVVYSTRSMAKLKNIPRKVEIVSEKDIEALDPVNATDLLK
CCCCCEEEEEECCCCCCCCCEEEEECHHHHHHHCCCCEEEEECCCCCHHCCCCCHHHHHH
KTSGVDIIEYPGVLSGVSIRGFSPDFSGIDQNVTYLIDGRPAGATNLATIDMNNIERVEV
HCCCCEEEECCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEE
IKGPSSALYGSQGMGGSINFITRKSAGALSGKASLGYSSFSTLDGSVRVGGSLSERFDVD
EECCCHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCEEEE
LGVRSYNQNEDYTVGSNTLISDPDPEVLEQDIDTMRNSTYATQSGNLRIGYQLNDEVRID
ECEECCCCCCCEECCCCCEECCCCHHHHHHHHHHHHCCCEEECCCCEEEEEEECCEEEEC
ARGEIYQAHSVKSPGSIWGVYGHSEKDIDRQTLDVTMTADWGTHALRFVPHWSLEKSVSY
CCCCEEEECCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCC
KKDDEGLNYRSSLSEIEWLGFQLQDRIRVGRQTFTGGVDYTVIDVFSRSFADSETEKAPS
CCCCCCCCHHHHHHHHHHHCHHHHHHHHHCHHHCCCCCCEEHHHHHHHHHCCCHHHCCCC
RPNQQQSSLGVFGEVGISLFDETVIANIGGRYDITSYQLKETPLFTTVETESEEYDNFNP
CCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCEEEEECCCEEEEEECCCHHCCCCCC
SVSVQYRFLPQCKIHASIGRAFVAPNPYEKAGFYVDDDGMETRGNPDLEPETSVTWDAGL
CEEEEEEECCCEEEEHHHCEEEECCCCHHHCCEEECCCCCCCCCCCCCCCCCCEEECCCE
TYESDNGGFRTDVTCFSTDWENFIQRTSAVDEFGEEYKTFENLNSAEMNGIELECSYDFG
EEECCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
AAHDERYSLRLFTNFTHLFRARSINDDGSEGDMLYVRRNRGSMGLEYDDFRFFSARLTAR
CCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEECCHHEEHHHHHHH
YIGDRFEQNWIGRYDEARPTLKDPVIEIPASLIFDMSMGFRIDDQNDVSLIAKNILDENY
HHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHEEECCCCEEECCCCCHHHHHHHHHCCCC
TEKDGYNMPGRSLGVRYSVEF
CCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]