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The map label for this gene is btuB [H]

Identifier: 194334084

GI number: 194334084

Start: 1374948

End: 1376852

Strand: Reverse

Name: btuB [H]

Synonym: Paes_1271

Alternate gene names: 194334084

Gene position: 1376852-1374948 (Counterclockwise)

Preceding gene: 194334085

Following gene: 194334083

Centisome position: 54.79

GC content: 47.3

Gene sequence:

>1905_bases
ATGATTGTATCCATGAAAAAGCAGTTTTTTCTGTTTGTAGCTGTCGGCCTGTTGGCGACGGGCGTTGTCAGGGGGGATGA
TGTTTCGGAGGGAAAAGCTCTGAAACGTTATAACAGCGACGAACTGGTCGTATCAGCAACGAAAACCCTGAATTCTATCA
GCGATGCAGGAGGCAGTTCAGTAACTGTCATCACCGCTGAGGAGATCGAAAATTCCGGTCAGCAAAGTGTTGAAGAGGTG
CTTAAAGGAAATCCCGGTATCGATATCGCCAGCAATGGGGGGCCGGGAACGAATACCAGTATTTTTTTGAGAGGTGCCGA
TTCAAAATATACGCTTGTCCTTATTGACGGTGTTCCGGCCAATGATCCTGCAGAAGGAAGTCGCTCACCAAACCTGTCTC
ATTTGACGACTGATAATATCGAGCGCATCGAGATTGTTCGCGGACCCGCAAGTCTTTTATTCGGATCCGGGGCTTCGGCA
GGTTTAATTAATATCATTACGAAAAAGGGCTCGTCAACCCCTGAAGCTTATGCCGGGATCGAAGGTGGATCGTATAGTAC
TTACAAGTATTATGCCGGTGCCAACGGCAGGAAAGGGATCTTTGACTATTCATTTTCTGCATCCCGTCTGAAAAGTGATG
GCTTTTCGGCTACTGATGAAGAGAATTCATGTGTCAATCCGGCAGGGGAGTCTTTTGAAGATGATGGATACGAAAATACC
TCTTTGTCCGGAAACATCGGCTTGCATCTGAATGAGCATGTCACCTTTGAAACGGTTATGAGGTATACCAATGCGAATTC
GCAATACGATGGTCCTGGTGTGGATGTTGTCGGTAACGAGCAGGACGCGGAACAGTTGAATGGTCGGGTTGCTTTGACAA
TGGATTACGAGCCTCTGGTGTCGACGATGTATTATAATTTCAACAGACAGGATCGCGATTACCTGACAGATGGAGCATTG
TCAAGTACGTATCACGGTTATCTCTATGAGTTGGGGTGGCAGGGTGATCTTGCTGTGGCTGATAACAATACCATCAGTGC
CGGCCTGAATTATCAGCATGAAAGCATGCAGAATGAGAGTTTCGGTATGTATGCTTCGGCTATTGATCAGGATATTTCCT
CGAACAGTATCTTTATTCAGGATCAGTGGCGTTCAGACGGGGTGCAACTGATCGGTGGCATTCGCTATGAGGACAATGAA
CAGTTCGGCGGTGAGACGACTTTCAGGTTCGCCCCTTCCTGGACTGTTGACCATACGGTCTTTAAATGCAGTTACGGAAC
TGCCTTCAGGGCTCCGTCGCTTTACCAGCTGTATTCTCCTTATGGTAATGAGGATCTTTCTGCCGAGACCAGCAAAGGGT
GGGATGCAGGATTTGAACACAGCTTTTCGGACGATGTTAAAGTTGGGGTGACCTACTTCCGTATCGATTATGAGGATCGT
ATTGACTTTGATATGAACACATGGACCTATGCCCAGGTAGATGGCAAAACCAAAACCTTCGGGGTGGAGAGTTTTGCTGA
GTGGGCTGCGTCGAACGACTTTTTTCTCACGTTCAACTATACCTATACCCTGACTGAAGATCCCGATGGCAGTCAGTTGC
TGAGGCGTCCGAAAAATAAGGTCGCTTTGAGTGGAACCTATGATCTTTCCGAAAAGACGAAGCTGAGCACTAGCCTGAGA
TGGGTTGGATCGCGGAAGGATAGCGGAGCGAAAGACGAGAATTGTATCGTGACGAATAAGCTTGACAGTTATTTTCTCGT
CAATCTTTCGGGATCCTGTCAGCTGACTGATTCTATTGCATTGTATGGTCGGGTTGATAATCTGTTCGATGAGGATTATG
AAGATGCATGGCGATACGCTACTCCCGGCAGATCGGCTTATGCGGGGATCAAGCTTATCTATTGA

Upstream 100 bases:

>100_bases
TTATTTGTTCGTTCATGTTCTTTTTTCAAGGATCTCCTTCGTCATTTCTGCACTCTCTTAGTAGCTCCAGTCATGTAGTG
TTTCATTTGTGAATTAAAAC

Downstream 100 bases:

>100_bases
TTGATATTGTTGTTTGTTCGCATCAGGATATGAGCAATTACGTCCTGATGCGAGGGAGCATTGTAAAGATCTCTATTCTT
AATTAATTATATCAATTCAA

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MIVSMKKQFFLFVAVGLLATGVVRGDDVSEGKALKRYNSDELVVSATKTLNSISDAGGSSVTVITAEEIENSGQQSVEEV
LKGNPGIDIASNGGPGTNTSIFLRGADSKYTLVLIDGVPANDPAEGSRSPNLSHLTTDNIERIEIVRGPASLLFGSGASA
GLINIITKKGSSTPEAYAGIEGGSYSTYKYYAGANGRKGIFDYSFSASRLKSDGFSATDEENSCVNPAGESFEDDGYENT
SLSGNIGLHLNEHVTFETVMRYTNANSQYDGPGVDVVGNEQDAEQLNGRVALTMDYEPLVSTMYYNFNRQDRDYLTDGAL
SSTYHGYLYELGWQGDLAVADNNTISAGLNYQHESMQNESFGMYASAIDQDISSNSIFIQDQWRSDGVQLIGGIRYEDNE
QFGGETTFRFAPSWTVDHTVFKCSYGTAFRAPSLYQLYSPYGNEDLSAETSKGWDAGFEHSFSDDVKVGVTYFRIDYEDR
IDFDMNTWTYAQVDGKTKTFGVESFAEWAASNDFFLTFNYTYTLTEDPDGSQLLRRPKNKVALSGTYDLSEKTKLSTSLR
WVGSRKDSGAKDENCIVTNKLDSYFLVNLSGSCQLTDSIALYGRVDNLFDEDYEDAWRYATPGRSAYAGIKLIY

Sequences:

>Translated_634_residues
MIVSMKKQFFLFVAVGLLATGVVRGDDVSEGKALKRYNSDELVVSATKTLNSISDAGGSSVTVITAEEIENSGQQSVEEV
LKGNPGIDIASNGGPGTNTSIFLRGADSKYTLVLIDGVPANDPAEGSRSPNLSHLTTDNIERIEIVRGPASLLFGSGASA
GLINIITKKGSSTPEAYAGIEGGSYSTYKYYAGANGRKGIFDYSFSASRLKSDGFSATDEENSCVNPAGESFEDDGYENT
SLSGNIGLHLNEHVTFETVMRYTNANSQYDGPGVDVVGNEQDAEQLNGRVALTMDYEPLVSTMYYNFNRQDRDYLTDGAL
SSTYHGYLYELGWQGDLAVADNNTISAGLNYQHESMQNESFGMYASAIDQDISSNSIFIQDQWRSDGVQLIGGIRYEDNE
QFGGETTFRFAPSWTVDHTVFKCSYGTAFRAPSLYQLYSPYGNEDLSAETSKGWDAGFEHSFSDDVKVGVTYFRIDYEDR
IDFDMNTWTYAQVDGKTKTFGVESFAEWAASNDFFLTFNYTYTLTEDPDGSQLLRRPKNKVALSGTYDLSEKTKLSTSLR
WVGSRKDSGAKDENCIVTNKLDSYFLVNLSGSCQLTDSIALYGRVDNLFDEDYEDAWRYATPGRSAYAGIKLIY
>Mature_634_residues
MIVSMKKQFFLFVAVGLLATGVVRGDDVSEGKALKRYNSDELVVSATKTLNSISDAGGSSVTVITAEEIENSGQQSVEEV
LKGNPGIDIASNGGPGTNTSIFLRGADSKYTLVLIDGVPANDPAEGSRSPNLSHLTTDNIERIEIVRGPASLLFGSGASA
GLINIITKKGSSTPEAYAGIEGGSYSTYKYYAGANGRKGIFDYSFSASRLKSDGFSATDEENSCVNPAGESFEDDGYENT
SLSGNIGLHLNEHVTFETVMRYTNANSQYDGPGVDVVGNEQDAEQLNGRVALTMDYEPLVSTMYYNFNRQDRDYLTDGAL
SSTYHGYLYELGWQGDLAVADNNTISAGLNYQHESMQNESFGMYASAIDQDISSNSIFIQDQWRSDGVQLIGGIRYEDNE
QFGGETTFRFAPSWTVDHTVFKCSYGTAFRAPSLYQLYSPYGNEDLSAETSKGWDAGFEHSFSDDVKVGVTYFRIDYEDR
IDFDMNTWTYAQVDGKTKTFGVESFAEWAASNDFFLTFNYTYTLTEDPDGSQLLRRPKNKVALSGTYDLSEKTKLSTSLR
WVGSRKDSGAKDENCIVTNKLDSYFLVNLSGSCQLTDSIALYGRVDNLFDEDYEDAWRYATPGRSAYAGIKLIY

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=608, Percent_Identity=28.4539473684211, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1788478, Length=654, Percent_Identity=26.1467889908257, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787723, Length=708, Percent_Identity=21.8926553672316, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786798, Length=178, Percent_Identity=33.7078651685393, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786344, Length=207, Percent_Identity=25.1207729468599, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 69601; Mature: 69601

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVSMKKQFFLFVAVGLLATGVVRGDDVSEGKALKRYNSDELVVSATKTLNSISDAGGSS
CEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEHHHHHHHHCCCCCE
VTVITAEEIENSGQQSVEEVLKGNPGIDIASNGGPGTNTSIFLRGADSKYTLVLIDGVPA
EEEEEHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCC
NDPAEGSRSPNLSHLTTDNIERIEIVRGPASLLFGSGASAGLINIITKKGSSTPEAYAGI
CCCCCCCCCCCCCEEECCCCEEEEEEECCHHEEECCCCCCCEEEEEECCCCCCCHHHCCC
EGGSYSTYKYYAGANGRKGIFDYSFSASRLKSDGFSATDEENSCVNPAGESFEDDGYENT
CCCCCEEEEEEECCCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SLSGNIGLHLNEHVTFETVMRYTNANSQYDGPGVDVVGNEQDAEQLNGRVALTMDYEPLV
CCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCEEEEEECCHHHH
STMYYNFNRQDRDYLTDGALSSTYHGYLYELGWQGDLAVADNNTISAGLNYQHESMQNES
HHHEECCCCCCCHHHCCCCCCCCCCCEEEEECCCCCEEEECCCEEEECCCCCHHHCCCCC
FGMYASAIDQDISSNSIFIQDQWRSDGVQLIGGIRYEDNEQFGGETTFRFAPSWTVDHTV
CCHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCCEEEEECCCCEECCEE
FKCSYGTAFRAPSLYQLYSPYGNEDLSAETSKGWDAGFEHSFSDDVKVGVTYFRIDYEDR
EEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCC
IDFDMNTWTYAQVDGKTKTFGVESFAEWAASNDFFLTFNYTYTLTEDPDGSQLLRRPKNK
CCCCCCCEEEEEECCCCEEECHHHHHHHHCCCCEEEEEEEEEEEECCCCHHHHHHCCCCC
VALSGTYDLSEKTKLSTSLRWVGSRKDSGAKDENCIVTNKLDSYFLVNLSGSCQLTDSIA
EEEEEECCCCCCHHHHHEEEEECCCCCCCCCCCCEEEEECCCCEEEEEECCCEEEECCEE
LYGRVDNLFDEDYEDAWRYATPGRSAYAGIKLIY
EEEEHHHHHCCCHHHHHCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MIVSMKKQFFLFVAVGLLATGVVRGDDVSEGKALKRYNSDELVVSATKTLNSISDAGGSS
CEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEHHHHHHHHCCCCCE
VTVITAEEIENSGQQSVEEVLKGNPGIDIASNGGPGTNTSIFLRGADSKYTLVLIDGVPA
EEEEEHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCC
NDPAEGSRSPNLSHLTTDNIERIEIVRGPASLLFGSGASAGLINIITKKGSSTPEAYAGI
CCCCCCCCCCCCCEEECCCCEEEEEEECCHHEEECCCCCCCEEEEEECCCCCCCHHHCCC
EGGSYSTYKYYAGANGRKGIFDYSFSASRLKSDGFSATDEENSCVNPAGESFEDDGYENT
CCCCCEEEEEEECCCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SLSGNIGLHLNEHVTFETVMRYTNANSQYDGPGVDVVGNEQDAEQLNGRVALTMDYEPLV
CCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCEEEEEECCHHHH
STMYYNFNRQDRDYLTDGALSSTYHGYLYELGWQGDLAVADNNTISAGLNYQHESMQNES
HHHEECCCCCCCHHHCCCCCCCCCCCEEEEECCCCCEEEECCCEEEECCCCCHHHCCCCC
FGMYASAIDQDISSNSIFIQDQWRSDGVQLIGGIRYEDNEQFGGETTFRFAPSWTVDHTV
CCHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCCEEEEECCCCEECCEE
FKCSYGTAFRAPSLYQLYSPYGNEDLSAETSKGWDAGFEHSFSDDVKVGVTYFRIDYEDR
EEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCC
IDFDMNTWTYAQVDGKTKTFGVESFAEWAASNDFFLTFNYTYTLTEDPDGSQLLRRPKNK
CCCCCCCEEEEEECCCCEEECHHHHHHHHCCCCEEEEEEEEEEEECCCCHHHHHHCCCCC
VALSGTYDLSEKTKLSTSLRWVGSRKDSGAKDENCIVTNKLDSYFLVNLSGSCQLTDSIA
EEEEEECCCCCCHHHHHEEEEECCCCCCCCCCCCEEEEECCCCEEEEEECCCEEEECCEE
LYGRVDNLFDEDYEDAWRYATPGRSAYAGIKLIY
EEEEHHHHHCCCHHHHHCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA