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The map label for this gene is ispB [H]

Identifier: 194334004

GI number: 194334004

Start: 1296817

End: 1297791

Strand: Reverse

Name: ispB [H]

Synonym: Paes_1188

Alternate gene names: 194334004

Gene position: 1297791-1296817 (Counterclockwise)

Preceding gene: 194334005

Following gene: 194334003

Centisome position: 51.64

GC content: 48.72

Gene sequence:

>975_bases
GTGGATATTAAAGAGGTAACCGTATCGGTCTCTCAGGAGCTCGAAACCTTTCGTCAGCGCTATAAAGTTGCGCTGAAAAG
TGATAACAGCCTGATTGACAAGGTTATGCGCTACATTCTCAAGCAGCAGGGCAAGCAGGTTCGCCCGCTTCTGGTCATGC
TCGGCGCCAAAGTGTGCGGCGGCGTAGCAGACGCCTCTTACAGGGCGGCGATAATGGTCGAGTTGCTTCACTCGGCAACA
CTCGTACACGATGATGTGGTTGACGGGGCTGAGATGAGACGGGGATTGGCTTCGATCAATGCCGTGTGGAAAAATAAGAT
CGCCGTACTCATCGGTGACTACATGCTTTCCAAGGGTCTGCTTTATTCTCTTGATCATAAAGATTACCGATCGCTCCATA
TGGTGTCGGATGCAGTGCGCAGGATGAGCGAGGGTGAGATTCTGCAGATTCAGAAAACACGAAGCCTCGATATTTCCGAA
GAGGATTATCTCAAGGTTATTTCTGATAAAACCGCCTCGTTACTGTCGACTTCATGCGCAATGGGGGGGGCGAGCGTGAC
TGATGATGAGGAAAAGATCGAAGCGCTCAGGAATTACGGTGAGTTTCTCGGGCTTGCTTTTCAGATCCGCGACGATCTGC
TTGATTATACCGGGGATTCAAATAAAACCGGTAAACAGCTCGGAATAGATATTCAGGATAAAAAAATCACACTTCCGCTC
ATCTATGCGCTTGGCCAGGCTGAACGTTCCGAGCAGAAAAGAATTCGTTCAATTCTCAAGAGTTCGAAGAAACGTGCTAT
GAAAAGCGGGGAGGTGATGGATTTTGTTCGAAAAAGCGGGGGACTGGAATATTCTTCAGAGGTTGCGGAAGGATTTGCAT
CAAAAGCGACTGCGGCACTTGAGGTTTTTGATGATTCCGAAGCTCGCAGATCCCTGGAGCTTCTTGTCGACTTTGTGATG
AAGCGTCAACATTAA

Upstream 100 bases:

>100_bases
TTGGGCTGAGAAATGTATACTTTTCTAAAATTTTTCTTTATGCCGGTACGTCCTTACTGCACCAGGCCGGAACAACGCTG
ATTATAAAGCAAAGGAAACT

Downstream 100 bases:

>100_bases
TGAGAACGATTTGAGGGGACTACTCTGCATATGAATACCATGATGAAGAAAGCTGCAGTAATGGTGGCCGTTTTATTGGT
GCTGCTGTTGGCTGTGGTGC

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MDIKEVTVSVSQELETFRQRYKVALKSDNSLIDKVMRYILKQQGKQVRPLLVMLGAKVCGGVADASYRAAIMVELLHSAT
LVHDDVVDGAEMRRGLASINAVWKNKIAVLIGDYMLSKGLLYSLDHKDYRSLHMVSDAVRRMSEGEILQIQKTRSLDISE
EDYLKVISDKTASLLSTSCAMGGASVTDDEEKIEALRNYGEFLGLAFQIRDDLLDYTGDSNKTGKQLGIDIQDKKITLPL
IYALGQAERSEQKRIRSILKSSKKRAMKSGEVMDFVRKSGGLEYSSEVAEGFASKATAALEVFDDSEARRSLELLVDFVM
KRQH

Sequences:

>Translated_324_residues
MDIKEVTVSVSQELETFRQRYKVALKSDNSLIDKVMRYILKQQGKQVRPLLVMLGAKVCGGVADASYRAAIMVELLHSAT
LVHDDVVDGAEMRRGLASINAVWKNKIAVLIGDYMLSKGLLYSLDHKDYRSLHMVSDAVRRMSEGEILQIQKTRSLDISE
EDYLKVISDKTASLLSTSCAMGGASVTDDEEKIEALRNYGEFLGLAFQIRDDLLDYTGDSNKTGKQLGIDIQDKKITLPL
IYALGQAERSEQKRIRSILKSSKKRAMKSGEVMDFVRKSGGLEYSSEVAEGFASKATAALEVFDDSEARRSLELLVDFVM
KRQH
>Mature_324_residues
MDIKEVTVSVSQELETFRQRYKVALKSDNSLIDKVMRYILKQQGKQVRPLLVMLGAKVCGGVADASYRAAIMVELLHSAT
LVHDDVVDGAEMRRGLASINAVWKNKIAVLIGDYMLSKGLLYSLDHKDYRSLHMVSDAVRRMSEGEILQIQKTRSLDISE
EDYLKVISDKTASLLSTSCAMGGASVTDDEEKIEALRNYGEFLGLAFQIRDDLLDYTGDSNKTGKQLGIDIQDKKITLPL
IYALGQAERSEQKRIRSILKSSKKRAMKSGEVMDFVRKSGGLEYSSEVAEGFASKATAALEVFDDSEARRSLELLVDFVM
KRQH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=293, Percent_Identity=29.6928327645051, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI4758430, Length=271, Percent_Identity=24.7232472324723, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI83700220, Length=271, Percent_Identity=24.7232472324723, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1789578, Length=324, Percent_Identity=34.8765432098765, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1786623, Length=281, Percent_Identity=27.7580071174377, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=250, Percent_Identity=31.2, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319475, Length=308, Percent_Identity=25.974025974026, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6325188, Length=283, Percent_Identity=24.3816254416961, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24651612, Length=324, Percent_Identity=28.0864197530864, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36173; Mature: 36173

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKEVTVSVSQELETFRQRYKVALKSDNSLIDKVMRYILKQQGKQVRPLLVMLGAKVCG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GVADASYRAAIMVELLHSATLVHDDVVDGAEMRRGLASINAVWKNKIAVLIGDYMLSKGL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LYSLDHKDYRSLHMVSDAVRRMSEGEILQIQKTRSLDISEEDYLKVISDKTASLLSTSCA
EEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MGGASVTDDEEKIEALRNYGEFLGLAFQIRDDLLDYTGDSNKTGKQLGIDIQDKKITLPL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCEEHHHH
IYALGQAERSEQKRIRSILKSSKKRAMKSGEVMDFVRKSGGLEYSSEVAEGFASKATAAL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EVFDDSEARRSLELLVDFVMKRQH
HHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIKEVTVSVSQELETFRQRYKVALKSDNSLIDKVMRYILKQQGKQVRPLLVMLGAKVCG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GVADASYRAAIMVELLHSATLVHDDVVDGAEMRRGLASINAVWKNKIAVLIGDYMLSKGL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LYSLDHKDYRSLHMVSDAVRRMSEGEILQIQKTRSLDISEEDYLKVISDKTASLLSTSCA
EEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MGGASVTDDEEKIEALRNYGEFLGLAFQIRDDLLDYTGDSNKTGKQLGIDIQDKKITLPL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCEEHHHH
IYALGQAERSEQKRIRSILKSSKKRAMKSGEVMDFVRKSGGLEYSSEVAEGFASKATAAL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EVFDDSEARRSLELLVDFVMKRQH
HHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]