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The map label for this gene is sbcD [H]

Identifier: 194333975

GI number: 194333975

Start: 1263234

End: 1264466

Strand: Reverse

Name: sbcD [H]

Synonym: Paes_1159

Alternate gene names: 194333975

Gene position: 1264466-1263234 (Counterclockwise)

Preceding gene: 194333976

Following gene: 194333974

Centisome position: 50.32

GC content: 53.12

Gene sequence:

>1233_bases
ATGAAATTTCTGCATACATCGGACTGGCATATTGGCGCAATGCTCTACGGCCGGAAGCGCTACGAAGAGTATGAACGCTT
CCTTGACTGGATGGTCATGACGGTTGAATCCGAATCGGTTGACCTGCTCATGATCGCCGGAGATATCTTTCACACAACGA
CACCCGGCAGCAGAGCGCAGTCGCTCTATTACAATTTTCTTGACCGCATTGCGGCTTCCAGCTGTCGTCATGTGGTCATA
ATCGCAGGTAATCATGATTCCCCTTCGCTGCTCGACGCTCCGCGTGCGCTTCTCCGGGCTCTGGATATCCATGTCTCAGG
AACCGCTTCTGCTGATCCTGCCGATGAGGTTATCGTGCTGAACCGTGCAGATGGTGAGCCGGAAGCAGTTGTTTGCGCTG
TGCCGTATCTCAGGGACCGCGACATCAGACTTGCCGGAGCGGGGGAACACGGTGAAGAGAAAACACAGCGTCTCATCGAT
GCCATTACAGCTCATTACGCACTGGTCTGTGATGCAGCCGAAGCTCGACGCCGTTCGATTGGTCAGGATATCCCCCTTCT
TGTCATGGGTCATCTTTTTGCTGCGGGAGGTGTCACAGCCGACGGTGATGGTGTTCGTGAAATCTATGTCGGCTCTCTGG
CAAGAGTGCCTGTTCAGGCCTTTCCTCCTGTGATAGACTATCTTGCTCTGGGGCATCTGCATGCGTCCCAGCGTCTTGCT
GGAAAAGAGTGTATGCGTTACAGCGGAGCACCTCTTCCTATGGGATTTGGTGAGGCTGCCCGGTCAGGAAAGATTGTCCT
CGGGGAAACCTCGGGAAGGGAGATCTCTATCAAAGAGATTGACGTTCCCTGTTTTCAGCATCTTGAACGGTTAGAAGGGA
CTTTCGAAGAGCTAACTCTTGCACTGCAGGCCATGCGGCAGCAAGCCTCCGGAAGCGTATGGATTGAAATTGATTACAAG
GGATCGATGCCGGCAGGAAGTCTGCGAGAGGAGCTTGATGCGATTGTCGAGGGAAGCCAAATCGAGATTCTCAGGATAAG
GAACCTTCGGGTGACCGATATCATGCTTGAGCAGATGGAGCAGGATGAGGAACTTGACGATCTCTCCGAAGAGGAGGTTT
TTTCCCGTTGCATGGATAGCTGTGACGTTCCCGCTGATGAGCAGAGGTACCTTATGCAGGCCTATCGGGAAATTCTCTGT
TCCCTTCACGAAGAAGACCGGATGGAGGAGTAG

Upstream 100 bases:

>100_bases
TATCGGGCCAAAAGAGTTGAAAAACAGGAGTGAAATCTCTATTCTGCACCCTGGAGTTGCGATAGATAAAGGTGCGTTTT
TCTGATCCGGACAACTTGCT

Downstream 100 bases:

>100_bases
CTTCAAGCGTATGAAGATTTTGCAGTTATGTTTCAGAAACCTGAATTCGCTTCAGGGAACATGGTCGATCGACTTTACGG
CACCGCCATACGCTTCGGAC

Product: nuclease SbcCD subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKFLHTSDWHIGAMLYGRKRYEEYERFLDWMVMTVESESVDLLMIAGDIFHTTTPGSRAQSLYYNFLDRIAASSCRHVVI
IAGNHDSPSLLDAPRALLRALDIHVSGTASADPADEVIVLNRADGEPEAVVCAVPYLRDRDIRLAGAGEHGEEKTQRLID
AITAHYALVCDAAEARRRSIGQDIPLLVMGHLFAAGGVTADGDGVREIYVGSLARVPVQAFPPVIDYLALGHLHASQRLA
GKECMRYSGAPLPMGFGEAARSGKIVLGETSGREISIKEIDVPCFQHLERLEGTFEELTLALQAMRQQASGSVWIEIDYK
GSMPAGSLREELDAIVEGSQIEILRIRNLRVTDIMLEQMEQDEELDDLSEEEVFSRCMDSCDVPADEQRYLMQAYREILC
SLHEEDRMEE

Sequences:

>Translated_410_residues
MKFLHTSDWHIGAMLYGRKRYEEYERFLDWMVMTVESESVDLLMIAGDIFHTTTPGSRAQSLYYNFLDRIAASSCRHVVI
IAGNHDSPSLLDAPRALLRALDIHVSGTASADPADEVIVLNRADGEPEAVVCAVPYLRDRDIRLAGAGEHGEEKTQRLID
AITAHYALVCDAAEARRRSIGQDIPLLVMGHLFAAGGVTADGDGVREIYVGSLARVPVQAFPPVIDYLALGHLHASQRLA
GKECMRYSGAPLPMGFGEAARSGKIVLGETSGREISIKEIDVPCFQHLERLEGTFEELTLALQAMRQQASGSVWIEIDYK
GSMPAGSLREELDAIVEGSQIEILRIRNLRVTDIMLEQMEQDEELDDLSEEEVFSRCMDSCDVPADEQRYLMQAYREILC
SLHEEDRMEE
>Mature_410_residues
MKFLHTSDWHIGAMLYGRKRYEEYERFLDWMVMTVESESVDLLMIAGDIFHTTTPGSRAQSLYYNFLDRIAASSCRHVVI
IAGNHDSPSLLDAPRALLRALDIHVSGTASADPADEVIVLNRADGEPEAVVCAVPYLRDRDIRLAGAGEHGEEKTQRLID
AITAHYALVCDAAEARRRSIGQDIPLLVMGHLFAAGGVTADGDGVREIYVGSLARVPVQAFPPVIDYLALGHLHASQRLA
GKECMRYSGAPLPMGFGEAARSGKIVLGETSGREISIKEIDVPCFQHLERLEGTFEELTLALQAMRQQASGSVWIEIDYK
GSMPAGSLREELDAIVEGSQIEILRIRNLRVTDIMLEQMEQDEELDDLSEEEVFSRCMDSCDVPADEQRYLMQAYREILC
SLHEEDRMEE

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=405, Percent_Identity=35.0617283950617, Blast_Score=254, Evalue=9e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 45642; Mature: 45642

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLHTSDWHIGAMLYGRKRYEEYERFLDWMVMTVESESVDLLMIAGDIFHTTTPGSRAQ
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCHHCCCCCCHHHH
SLYYNFLDRIAASSCRHVVIIAGNHDSPSLLDAPRALLRALDIHVSGTASADPADEVIVL
HHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEE
NRADGEPEAVVCAVPYLRDRDIRLAGAGEHGEEKTQRLIDAITAHYALVCDAAEARRRSI
ECCCCCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHC
GQDIPLLVMGHLFAAGGVTADGDGVREIYVGSLARVPVQAFPPVIDYLALGHLHASQRLA
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GKECMRYSGAPLPMGFGEAARSGKIVLGETSGREISIKEIDVPCFQHLERLEGTFEELTL
HHHHHHHCCCCCCCCCCHHCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHCCHHHHHHH
ALQAMRQQASGSVWIEIDYKGSMPAGSLREELDAIVEGSQIEILRIRNLRVTDIMLEQME
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
QDEELDDLSEEEVFSRCMDSCDVPADEQRYLMQAYREILCSLHEEDRMEE
CCCHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKFLHTSDWHIGAMLYGRKRYEEYERFLDWMVMTVESESVDLLMIAGDIFHTTTPGSRAQ
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCHHCCCCCCHHHH
SLYYNFLDRIAASSCRHVVIIAGNHDSPSLLDAPRALLRALDIHVSGTASADPADEVIVL
HHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEE
NRADGEPEAVVCAVPYLRDRDIRLAGAGEHGEEKTQRLIDAITAHYALVCDAAEARRRSI
ECCCCCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHC
GQDIPLLVMGHLFAAGGVTADGDGVREIYVGSLARVPVQAFPPVIDYLALGHLHASQRLA
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GKECMRYSGAPLPMGFGEAARSGKIVLGETSGREISIKEIDVPCFQHLERLEGTFEELTL
HHHHHHHCCCCCCCCCCHHCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHCCHHHHHHH
ALQAMRQQASGSVWIEIDYKGSMPAGSLREELDAIVEGSQIEILRIRNLRVTDIMLEQME
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
QDEELDDLSEEEVFSRCMDSCDVPADEQRYLMQAYREILCSLHEEDRMEE
CCCHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]