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The map label for this gene is mscS [H]

Identifier: 194333955

GI number: 194333955

Start: 1243413

End: 1244297

Strand: Reverse

Name: mscS [H]

Synonym: Paes_1139

Alternate gene names: 194333955

Gene position: 1244297-1243413 (Counterclockwise)

Preceding gene: 194333956

Following gene: 194333954

Centisome position: 49.52

GC content: 45.65

Gene sequence:

>885_bases
GTGGAATCGTTATCGCAGAGTCTGATTTCCCGCCTTAAAGACTACTTTAATGCGGAAAAAATGGGGCTGGTTCTAGGCGA
GATTCTTATCGAAACGCTTATCGCTGTTTCAATTCTCTCGGTATTTTATCTCTTCTGGAGAATTATCAAATGGATTGTTG
GCAGGCGTCTTGAAGAGCGTTTCGACAAAACAAGTGCAGCTTTTGCCGAAGCAGTCATCAAATTCACCATATTCGCCATT
GCGATCATTTCGGCACTTGGTGCCGCCGGAGTAAAAACCGATGCATTGCTTGGTTCACTTGGCGTTTTAGGTCTGACTCT
TGGTTTTGCTTTACGCGATACGCTCTCCAATATCGTTTCCGGTATACTGATCTTTCTGGATCGCCCATTCACCATCAACG
ATCTGGTTGAAATTGATGGAAAGTATGGCCGAATTGACAGGATAACCCTTCGAACGACGAGAGTCGTCACGAACGACGGC
AAGATGCTGGCTGTGCCGAATGCAGAAATCATGAACAAGACCGTCACGTCTTACACCAATTTTCCCCATCTTCGCATCGA
CGTCGGCGTAACTATCGCAGTAACGGAGAGTATTGATCTCGCCCGGAAGCTTTTATTGAAGCTGGTCAAAGATGATCCTG
ATTTCATGCAGCAACCGCTTCCTGTTGTGGTCGTGACGGAGCTTAACGATTATAACGTTGTTTTAGAGCTGGAGGCATGG
CTTCAGGATGAACGTCGTCATATTGAAAAACGTTGTGAAATCAGGGAAAAGATTTTCAGGACTTTTACGAAAGAAGGTAT
CGACATGCCGTATGAAACCATCCGGATTGTGCCACGCAACGAATGCGTTTCTTTGGCGGATGACGGAAAAAACAGTATGA
CGTAA

Upstream 100 bases:

>100_bases
TTCTTATGGTAAGCAGCATGTTTATCATGCCGATGAGCATCCTGATCGTCAACCACAATGGTTTTTTACCTCAAGCGATC
AAAGAGAGGGAGGCAAAAGA

Downstream 100 bases:

>100_bases
CCCAAAGCATATCAAATGACTGAAGCAACCCGACCTGACACGGTAAACGCAGATGCGCTTCCTGAAATCTGGCGATATGT
TGCAATCGACGATTTCAGCC

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MESLSQSLISRLKDYFNAEKMGLVLGEILIETLIAVSILSVFYLFWRIIKWIVGRRLEERFDKTSAAFAEAVIKFTIFAI
AIISALGAAGVKTDALLGSLGVLGLTLGFALRDTLSNIVSGILIFLDRPFTINDLVEIDGKYGRIDRITLRTTRVVTNDG
KMLAVPNAEIMNKTVTSYTNFPHLRIDVGVTIAVTESIDLARKLLLKLVKDDPDFMQQPLPVVVVTELNDYNVVLELEAW
LQDERRHIEKRCEIREKIFRTFTKEGIDMPYETIRIVPRNECVSLADDGKNSMT

Sequences:

>Translated_294_residues
MESLSQSLISRLKDYFNAEKMGLVLGEILIETLIAVSILSVFYLFWRIIKWIVGRRLEERFDKTSAAFAEAVIKFTIFAI
AIISALGAAGVKTDALLGSLGVLGLTLGFALRDTLSNIVSGILIFLDRPFTINDLVEIDGKYGRIDRITLRTTRVVTNDG
KMLAVPNAEIMNKTVTSYTNFPHLRIDVGVTIAVTESIDLARKLLLKLVKDDPDFMQQPLPVVVVTELNDYNVVLELEAW
LQDERRHIEKRCEIREKIFRTFTKEGIDMPYETIRIVPRNECVSLADDGKNSMT
>Mature_294_residues
MESLSQSLISRLKDYFNAEKMGLVLGEILIETLIAVSILSVFYLFWRIIKWIVGRRLEERFDKTSAAFAEAVIKFTIFAI
AIISALGAAGVKTDALLGSLGVLGLTLGFALRDTLSNIVSGILIFLDRPFTINDLVEIDGKYGRIDRITLRTTRVVTNDG
KMLAVPNAEIMNKTVTSYTNFPHLRIDVGVTIAVTESIDLARKLLLKLVKDDPDFMQQPLPVVVVTELNDYNVVLELEAW
LQDERRHIEKRCEIREKIFRTFTKEGIDMPYETIRIVPRNECVSLADDGKNSMT

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=243, Percent_Identity=26.7489711934156, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786670, Length=225, Percent_Identity=25.3333333333333, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI2367355, Length=206, Percent_Identity=22.8155339805825, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787591, Length=180, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 33143; Mature: 33143

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLSQSLISRLKDYFNAEKMGLVLGEILIETLIAVSILSVFYLFWRIIKWIVGRRLEER
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDKTSAAFAEAVIKFTIFAIAIISALGAAGVKTDALLGSLGVLGLTLGFALRDTLSNIVS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILIFLDRPFTINDLVEIDGKYGRIDRITLRTTRVVTNDGKMLAVPNAEIMNKTVTSYTN
HHHHHHCCCCCHHHHHHCCCCCCCEEEEEEEEEEEEECCCCEEECCCHHHHHHHHHHHCC
FPHLRIDVGVTIAVTESIDLARKLLLKLVKDDPDFMQQPLPVVVVTELNDYNVVLELEAW
CCEEEEEEEEEEEEECHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCEEEEEEHHH
LQDERRHIEKRCEIREKIFRTFTKEGIDMPYETIRIVPRNECVSLADDGKNSMT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MESLSQSLISRLKDYFNAEKMGLVLGEILIETLIAVSILSVFYLFWRIIKWIVGRRLEER
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDKTSAAFAEAVIKFTIFAIAIISALGAAGVKTDALLGSLGVLGLTLGFALRDTLSNIVS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILIFLDRPFTINDLVEIDGKYGRIDRITLRTTRVVTNDGKMLAVPNAEIMNKTVTSYTN
HHHHHHCCCCCHHHHHHCCCCCCCEEEEEEEEEEEEECCCCEEECCCHHHHHHHHHHHCC
FPHLRIDVGVTIAVTESIDLARKLLLKLVKDDPDFMQQPLPVVVVTELNDYNVVLELEAW
CCEEEEEEEEEEEEECHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCEEEEEEHHH
LQDERRHIEKRCEIREKIFRTFTKEGIDMPYETIRIVPRNECVSLADDGKNSMT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]