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The map label for this gene is pfkA2 [H]

Identifier: 194333890

GI number: 194333890

Start: 1176763

End: 1177806

Strand: Reverse

Name: pfkA2 [H]

Synonym: Paes_1068

Alternate gene names: 194333890

Gene position: 1177806-1176763 (Counterclockwise)

Preceding gene: 194333891

Following gene: 194333877

Centisome position: 46.87

GC content: 52.97

Gene sequence:

>1044_bases
ATGACCATTTCAGCAATGAGAGATCTATCTATGACACCTGCAACAGAGAACGGATTACGCAGAAAACCAATACTACGAAA
AATAGGAGTATTTACTTCTGGCGGGGATGCTCCCGGTATGAATGCCGCTATCCGCGCGGTCACTCGTGCGGCAATCGCGA
ACAAGCTGAAAGTCACCGGAATTCGCCGGGGATACCAGGGAATGATCGAGGGCGATTTTATTCCGTTGCGCTCATCGGAT
GTCAGCGGCATCATCCAGCTGGGAGGAACAATTCTCAGAACGGCACGGAGCAAGGAGTTCAGAACTCCCGAAGGCCGGGA
AAAAGCCTGGCAGCAGTTGCAGAACGCGGGCATAGACGCTGTTGTTGTGATCGGTGGCGACGGCTCTTTTACCGGTGCGC
TGACAATGTCTCAGGAGTACAATATATCGTTCGTAGGTATTCCGGGCACCATCGACAACGACATATACGGGACGGACTAT
ACCATCGGATATGAAACGGCATTGAACTCCGTTGTGTGCGCGGTCGACAAAATCAGGGATACGGCAGTATCACACGGCAG
GATTTTCATGGTTGAGGTGATGGGCCGGGAAGCAGGCCTGCTTGCCCTGAACAGCGGCATTGCCTGCGGGGCGGAAGTCA
TTCTGATTCCTGAATCGAAAGAGCAGCATGATGCGTTGAAGCGGTTTCTCGACAAGGGCTATAAGCGCAAGGAGCGAAGC
GGCATAGTTATCGTTGCCGAAGGGGATGAACCGGGAGGAGCGTTGAAACTTGCCGAAAACGTGCGCTGTGAGCATCCCGA
ACTTGATGTTCGCGTTTCGATCCTGGGCCATATTCAGCGAGGAGGAAGCCCGACGGCAAACGACCGCATCAATGCGACGA
GAATGGGAGTTGCTGCTGTCGATGCCCTGCTCGACGATCAGAAAAGCATCATGATCGGGATTGACAACGGAAGGATCGTG
CAGGTCCCGTTCAACAAGGCCGTCAAGCTGAATCACAGTTTCGACAGGGAATTACTGGAGATACAGAAAATGCTCAACAT
CTGA

Upstream 100 bases:

>100_bases
TGTTGAATGATGCGGATCATGACTGAATGAACAGCACTGCTCCTGATTTGCTGAGAGTCAATATTTTTGTACTATGACGT
TGCCGGAGGACCAACGCCTT

Downstream 100 bases:

>100_bases
GGCATTCCAGTCATCAATAAAGAAAAAAAACAAAAAAGTCACGTGACTATATAGAAAATGATCTCTAACAATCAATCATA
ATTATTGCCACCGCATAATG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTISAMRDLSMTPATENGLRRKPILRKIGVFTSGGDAPGMNAAIRAVTRAAIANKLKVTGIRRGYQGMIEGDFIPLRSSD
VSGIIQLGGTILRTARSKEFRTPEGREKAWQQLQNAGIDAVVVIGGDGSFTGALTMSQEYNISFVGIPGTIDNDIYGTDY
TIGYETALNSVVCAVDKIRDTAVSHGRIFMVEVMGREAGLLALNSGIACGAEVILIPESKEQHDALKRFLDKGYKRKERS
GIVIVAEGDEPGGALKLAENVRCEHPELDVRVSILGHIQRGGSPTANDRINATRMGVAAVDALLDDQKSIMIGIDNGRIV
QVPFNKAVKLNHSFDRELLEIQKMLNI

Sequences:

>Translated_347_residues
MTISAMRDLSMTPATENGLRRKPILRKIGVFTSGGDAPGMNAAIRAVTRAAIANKLKVTGIRRGYQGMIEGDFIPLRSSD
VSGIIQLGGTILRTARSKEFRTPEGREKAWQQLQNAGIDAVVVIGGDGSFTGALTMSQEYNISFVGIPGTIDNDIYGTDY
TIGYETALNSVVCAVDKIRDTAVSHGRIFMVEVMGREAGLLALNSGIACGAEVILIPESKEQHDALKRFLDKGYKRKERS
GIVIVAEGDEPGGALKLAENVRCEHPELDVRVSILGHIQRGGSPTANDRINATRMGVAAVDALLDDQKSIMIGIDNGRIV
QVPFNKAVKLNHSFDRELLEIQKMLNI
>Mature_346_residues
TISAMRDLSMTPATENGLRRKPILRKIGVFTSGGDAPGMNAAIRAVTRAAIANKLKVTGIRRGYQGMIEGDFIPLRSSDV
SGIIQLGGTILRTARSKEFRTPEGREKAWQQLQNAGIDAVVVIGGDGSFTGALTMSQEYNISFVGIPGTIDNDIYGTDYT
IGYETALNSVVCAVDKIRDTAVSHGRIFMVEVMGREAGLLALNSGIACGAEVILIPESKEQHDALKRFLDKGYKRKERSG
IVIVAEGDEPGGALKLAENVRCEHPELDVRVSILGHIQRGGSPTANDRINATRMGVAAVDALLDDQKSIMIGIDNGRIVQ
VPFNKAVKLNHSFDRELLEIQKMLNI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=350, Percent_Identity=41.7142857142857, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI266453619, Length=364, Percent_Identity=39.2857142857143, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI266453768, Length=364, Percent_Identity=39.2857142857143, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI266453748, Length=364, Percent_Identity=39.2857142857143, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI4505749, Length=364, Percent_Identity=39.2857142857143, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI48762920, Length=363, Percent_Identity=39.1184573002755, Blast_Score=218, Evalue=6e-57,
Organism=Escherichia coli, GI1790350, Length=323, Percent_Identity=44.5820433436532, Blast_Score=244, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI25147584, Length=339, Percent_Identity=43.952802359882, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17558788, Length=306, Percent_Identity=36.6013071895425, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI72003601, Length=293, Percent_Identity=29.3515358361775, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321679, Length=343, Percent_Identity=43.1486880466472, Blast_Score=228, Evalue=9e-61,
Organism=Saccharomyces cerevisiae, GI6323861, Length=361, Percent_Identity=38.2271468144044, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17647809, Length=355, Percent_Identity=42.5352112676056, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24652337, Length=350, Percent_Identity=42.2857142857143, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI28573326, Length=348, Percent_Identity=41.9540229885057, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 37489; Mature: 37357

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISAMRDLSMTPATENGLRRKPILRKIGVFTSGGDAPGMNAAIRAVTRAAIANKLKVTG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEECCCCCCCCHHHHHHHHHHHHHCCEEEEE
IRRGYQGMIEGDFIPLRSSDVSGIIQLGGTILRTARSKEFRTPEGREKAWQQLQNAGIDA
ECCCCCCCCCCCEEEECCCCCHHHHHHCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCE
VVVIGGDGSFTGALTMSQEYNISFVGIPGTIDNDIYGTDYTIGYETALNSVVCAVDKIRD
EEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
TAVSHGRIFMVEVMGREAGLLALNSGIACGAEVILIPESKEQHDALKRFLDKGYKRKERS
HHCCCCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHCCC
GIVIVAEGDEPGGALKLAENVRCEHPELDVRVSILGHIQRGGSPTANDRINATRMGVAAV
CEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCHHHHHHHHH
DALLDDQKSIMIGIDNGRIVQVPFNKAVKLNHSFDRELLEIQKMLNI
HHHHCCCCEEEEEECCCEEEEECCCCEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TISAMRDLSMTPATENGLRRKPILRKIGVFTSGGDAPGMNAAIRAVTRAAIANKLKVTG
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCEECCCCCCCCHHHHHHHHHHHHHCCEEEEE
IRRGYQGMIEGDFIPLRSSDVSGIIQLGGTILRTARSKEFRTPEGREKAWQQLQNAGIDA
ECCCCCCCCCCCEEEECCCCCHHHHHHCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCE
VVVIGGDGSFTGALTMSQEYNISFVGIPGTIDNDIYGTDYTIGYETALNSVVCAVDKIRD
EEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
TAVSHGRIFMVEVMGREAGLLALNSGIACGAEVILIPESKEQHDALKRFLDKGYKRKERS
HHCCCCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHCCC
GIVIVAEGDEPGGALKLAENVRCEHPELDVRVSILGHIQRGGSPTANDRINATRMGVAAV
CEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCHHHHHHHHH
DALLDDQKSIMIGIDNGRIVQVPFNKAVKLNHSFDRELLEIQKMLNI
HHHHCCCCEEEEEECCCEEEEECCCCEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]