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The map label for this gene is hldE [H]

Identifier: 194333839

GI number: 194333839

Start: 1115916

End: 1116899

Strand: Reverse

Name: hldE [H]

Synonym: Paes_1013

Alternate gene names: 194333839

Gene position: 1116899-1115916 (Counterclockwise)

Preceding gene: 194333847

Following gene: 194333835

Centisome position: 44.45

GC content: 51.73

Gene sequence:

>984_bases
ATGGATCTTGAAAAACTCTTCAACTCCTTCAGGAGCTGCAACATAGCAATTGTCGGCGATATCATGCTCGACAAATACAT
CTTCGGCCATGTCTCAAGGATATCGCCGGAATACCCCGTTCCTGTGGTCGATGTCTCGGAAGAAACATTCAGACTCGGAG
GAGCAGCAAATGTGGCCGTCAATATCAGAGCCATGGGCTGCAGAACCTCGCTTTTCGGTATTACCGGGGCAGACGCAGAC
AGCGACATTATGCTGGAACTCCTCGGAAAGCAGGATCTCACAACGTCGTCCATCATCAGCGATGCATCGCGACCGACAAC
CTGCAAGACAAGGATTCTATCACAAAACCACCATATCGTCAGGGTTGACCATGAGAGCCGCCACCATATTTCCGATGCAC
TCGAAGCAGTTATCCTTGATCGGATGAGGACATCGATAGATGATTTCGACGCAATTGTTCTCGAAGACTATAACAAGGGG
GTTCTGACCCCGCGTCTCATCAGAGAGATTATCGATCTGGCGACAGCAAAAGATGTTCCGGTGCTGGTTGATCCGAAACT
TGAGCGATTTTTTGACTATCAGGGCTGCAGCATCTTCAAACCAAACATTTCCGAAGTGGCCGCCTCACTGGGAATCATGC
CGCCTCAAGACGACACCGACATCGAAACCGCCTGCGTTCAGCTTAAAGAAAAACTGGGCTGTGAGCATCTGGTCATAACG
CGTGGCGAAAAAGGGATGACGATCTTCAATGACTCCTTTACCCATATTCCCGCAGCAACCCTCGACGTAGCCGATGTTTC
AGGCGCCGGTGACACCGTGATAGCAATGCTGGCGCTCGGTGCCGCATCAGGCCTCGACATCGTCACAAGCGCAGAAATTG
CGACGCTGGCGGCATCAACAGTCTGTCAGGAAGTCGGCGCGGCACCGGTAAAAACAGACAAGCTCCTCAAAGCCTGTCGC
GACTACTTTGGCAGAAACCTCTGA

Upstream 100 bases:

>100_bases
AACCTATCGTATGGATTATACGTAAACCAACAGCTTTTTATTCCCTGACTCTTCGTATATTCCGGTTTTGAACGACAACG
CTCAGACCGGCCTTCCAGAT

Downstream 100 bases:

>100_bases
AACGAACGTTCTAATAATGGGCCCCTGCCAGACCACGACACGAAGGTTCGAGGCAATGATGATGAGAAAAAAAAGACTGT
TCGAATGATGCCGGGGCAGT

Product: rfaE bifunctional protein

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MDLEKLFNSFRSCNIAIVGDIMLDKYIFGHVSRISPEYPVPVVDVSEETFRLGGAANVAVNIRAMGCRTSLFGITGADAD
SDIMLELLGKQDLTTSSIISDASRPTTCKTRILSQNHHIVRVDHESRHHISDALEAVILDRMRTSIDDFDAIVLEDYNKG
VLTPRLIREIIDLATAKDVPVLVDPKLERFFDYQGCSIFKPNISEVAASLGIMPPQDDTDIETACVQLKEKLGCEHLVIT
RGEKGMTIFNDSFTHIPAATLDVADVSGAGDTVIAMLALGAASGLDIVTSAEIATLAASTVCQEVGAAPVKTDKLLKACR
DYFGRNL

Sequences:

>Translated_327_residues
MDLEKLFNSFRSCNIAIVGDIMLDKYIFGHVSRISPEYPVPVVDVSEETFRLGGAANVAVNIRAMGCRTSLFGITGADAD
SDIMLELLGKQDLTTSSIISDASRPTTCKTRILSQNHHIVRVDHESRHHISDALEAVILDRMRTSIDDFDAIVLEDYNKG
VLTPRLIREIIDLATAKDVPVLVDPKLERFFDYQGCSIFKPNISEVAASLGIMPPQDDTDIETACVQLKEKLGCEHLVIT
RGEKGMTIFNDSFTHIPAATLDVADVSGAGDTVIAMLALGAASGLDIVTSAEIATLAASTVCQEVGAAPVKTDKLLKACR
DYFGRNL
>Mature_327_residues
MDLEKLFNSFRSCNIAIVGDIMLDKYIFGHVSRISPEYPVPVVDVSEETFRLGGAANVAVNIRAMGCRTSLFGITGADAD
SDIMLELLGKQDLTTSSIISDASRPTTCKTRILSQNHHIVRVDHESRHHISDALEAVILDRMRTSIDDFDAIVLEDYNKG
VLTPRLIREIIDLATAKDVPVLVDPKLERFFDYQGCSIFKPNISEVAASLGIMPPQDDTDIETACVQLKEKLGCEHLVIT
RGEKGMTIFNDSFTHIPAATLDVADVSGAGDTVIAMLALGAASGLDIVTSAEIATLAASTVCQEVGAAPVKTDKLLKACR
DYFGRNL

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=313, Percent_Identity=35.4632587859425, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.- [C]

Molecular weight: Translated: 35456; Mature: 35456

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLEKLFNSFRSCNIAIVGDIMLDKYIFGHVSRISPEYPVPVVDVSEETFRLGGAANVAV
CCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHCCCCCEEEE
NIRAMGCRTSLFGITGADADSDIMLELLGKQDLTTSSIISDASRPTTCKTRILSQNHHIV
EEEEECCCEEECEEECCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHCCCCCEE
RVDHESRHHISDALEAVILDRMRTSIDDFDAIVLEDYNKGVLTPRLIREIIDLATAKDVP
EECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCC
VLVDPKLERFFDYQGCSIFKPNISEVAASLGIMPPQDDTDIETACVQLKEKLGCEHLVIT
EEECCHHHHHHCCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
RGEKGMTIFNDSFTHIPAATLDVADVSGAGDTVIAMLALGAASGLDIVTSAEIATLAAST
CCCCCCEEECCCCCCCCCCEEEHHCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
VCQEVGAAPVKTDKLLKACRDYFGRNL
HHHHHCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLEKLFNSFRSCNIAIVGDIMLDKYIFGHVSRISPEYPVPVVDVSEETFRLGGAANVAV
CCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHCCCCCEEEE
NIRAMGCRTSLFGITGADADSDIMLELLGKQDLTTSSIISDASRPTTCKTRILSQNHHIV
EEEEECCCEEECEEECCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHCCCCCEE
RVDHESRHHISDALEAVILDRMRTSIDDFDAIVLEDYNKGVLTPRLIREIIDLATAKDVP
EECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCC
VLVDPKLERFFDYQGCSIFKPNISEVAASLGIMPPQDDTDIETACVQLKEKLGCEHLVIT
EEECCHHHHHHCCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
RGEKGMTIFNDSFTHIPAATLDVADVSGAGDTVIAMLALGAASGLDIVTSAEIATLAAST
CCCCCCEEECCCCCCCCCCEEEHHCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
VCQEVGAAPVKTDKLLKACRDYFGRNL
HHHHHCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA