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The map label for this gene is trmH [H]

Identifier: 194333759

GI number: 194333759

Start: 1022634

End: 1023284

Strand: Reverse

Name: trmH [H]

Synonym: Paes_0929

Alternate gene names: 194333759

Gene position: 1023284-1022634 (Counterclockwise)

Preceding gene: 194333765

Following gene: 194333757

Centisome position: 40.72

GC content: 49.31

Gene sequence:

>651_bases
TTGATAAATCCAGAACGATTCCATAAAATTCGACGCCTGCTCGAACAACGCCAGCCAGACCTGACCGTGGTCATGGACAA
TGTCAATAAACCGCATAATCTTGCAGCCATCATACGAACATGCGATTCGGTCGGGATAGAAACGATCCACGCAGTATCAA
ACAGGCCCTCTATCAGAAAGAAACAGAAAACAGCGGGCGGCTCGACAAGATGGGTTCAGCTCTCTCTCCATGAATCCATG
GAAAACCTTGCCAATACGCTCAAAAGCAACGGAACCCGTCTTCTGGCAGCACATTACTCCAGCACATCGGTAGATTTCCG
AAGCATCGACTACACCCGGCCAACAGCGATCGTCATGGGTGCAGAAAAACTCGGTCCATCTTCAGATACGATCAGCCTTG
TTGATCAGGCGATCCACATTCCAATGCAAGGCATGGCAGAATCCCTCAATGTCTCGGTCGCTGCAGCAGTTATTCTTTTT
GAAGCCCAACGCCAAAGACTTGCTGCCGGCTGCTATGAAAAAATCGCTCTTGAAAAAGAGACTCTCGAAAAAAGGCTCTT
CGAACTTGCCTACCCGTCTATAGCCGAAAAGTTGGCTATGCAGGCAAAACCCTATCCGCCCCTTGATGAAGAGGGGCGAC
TGCTTGATTGA

Upstream 100 bases:

>100_bases
GAACCATGTCGCTTCAGCGAATTGTTCATTTTCGGATTTTTTTTCTCCTGAAGCAAGTACCTCTTGAAGAACATTTTTCA
CCAGCAGAATATTCCCGCTT

Downstream 100 bases:

>100_bases
GCTGCATGGTCTCTGTCTCGAACAATAAAACAGGAAAAACGGCCGGCCCCCGACTCCTGCTGAAGATGACGGTGCAGACC
ATGCAGTAACAACTCGATTT

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MINPERFHKIRRLLEQRQPDLTVVMDNVNKPHNLAAIIRTCDSVGIETIHAVSNRPSIRKKQKTAGGSTRWVQLSLHESM
ENLANTLKSNGTRLLAAHYSSTSVDFRSIDYTRPTAIVMGAEKLGPSSDTISLVDQAIHIPMQGMAESLNVSVAAAVILF
EAQRQRLAAGCYEKIALEKETLEKRLFELAYPSIAEKLAMQAKPYPPLDEEGRLLD

Sequences:

>Translated_216_residues
MINPERFHKIRRLLEQRQPDLTVVMDNVNKPHNLAAIIRTCDSVGIETIHAVSNRPSIRKKQKTAGGSTRWVQLSLHESM
ENLANTLKSNGTRLLAAHYSSTSVDFRSIDYTRPTAIVMGAEKLGPSSDTISLVDQAIHIPMQGMAESLNVSVAAAVILF
EAQRQRLAAGCYEKIALEKETLEKRLFELAYPSIAEKLAMQAKPYPPLDEEGRLLD
>Mature_216_residues
MINPERFHKIRRLLEQRQPDLTVVMDNVNKPHNLAAIIRTCDSVGIETIHAVSNRPSIRKKQKTAGGSTRWVQLSLHESM
ENLANTLKSNGTRLLAAHYSSTSVDFRSIDYTRPTAIVMGAEKLGPSSDTISLVDQAIHIPMQGMAESLNVSVAAAVILF
EAQRQRLAAGCYEKIALEKETLEKRLFELAYPSIAEKLAMQAKPYPPLDEEGRLLD

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=215, Percent_Identity=46.9767441860465, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1790623, Length=154, Percent_Identity=35.0649350649351, Blast_Score=69, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355819, Length=152, Percent_Identity=28.2894736842105, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 24048; Mature: 24048

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPERFHKIRRLLEQRQPDLTVVMDNVNKPHNLAAIIRTCDSVGIETIHAVSNRPSIRK
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCHHH
KQKTAGGSTRWVQLSLHESMENLANTLKSNGTRLLAAHYSSTSVDFRSIDYTRPTAIVMG
HHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCEEEEEE
AEKLGPSSDTISLVDQAIHIPMQGMAESLNVSVAAAVILFEAQRQRLAAGCYEKIALEKE
HHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLEKRLFELAYPSIAEKLAMQAKPYPPLDEEGRLLD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MINPERFHKIRRLLEQRQPDLTVVMDNVNKPHNLAAIIRTCDSVGIETIHAVSNRPSIRK
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCHHH
KQKTAGGSTRWVQLSLHESMENLANTLKSNGTRLLAAHYSSTSVDFRSIDYTRPTAIVMG
HHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCEEEEEE
AEKLGPSSDTISLVDQAIHIPMQGMAESLNVSVAAAVILFEAQRQRLAAGCYEKIALEKE
HHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLEKRLFELAYPSIAEKLAMQAKPYPPLDEEGRLLD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]