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The map label for this gene is prfC [H]

Identifier: 194333731

GI number: 194333731

Start: 985589

End: 987178

Strand: Reverse

Name: prfC [H]

Synonym: Paes_0901

Alternate gene names: 194333731

Gene position: 987178-985589 (Counterclockwise)

Preceding gene: 194333738

Following gene: 194333730

Centisome position: 39.28

GC content: 48.36

Gene sequence:

>1590_bases
ATGAGCATTGAAAAAGAAATAGCAAAGAGAAGAACCTTTGCAATCATCAGTCATCCTGATGCCGGTAAAACAACTCTCAC
AGAAAAATTCCTGCTCTTTGGCGGTGCCATTCAAACCGCCGGAGCTGTCAAACAGAACAAGATTCGCAAAACAGCGACAA
GCGATTTCATGGAGATCGAAAAACAGAGAGGCATCTCTGTTGCAACCTCGGTAATGGGATTTGAATACAACGGTATACGG
ATCAATATTCTCGACACACCGGGCCATAAAGACTTTGCCGAAGACACCTACCGAACCCTTACCGCCGTCGATAGCGTCAT
TCTGGTTGTCGACTGCGTCAAAGGCGTTGAAGAGCAGACCGAAAAACTGATGGAGGTCTGCCGAATGCGCAAGACACCCG
TCATCATCTTCATCAACAAAATGGATCGCGAGGGCAAAAATCCCGTTGAACTGCTCGATGAACTTGAAGAAAAACTGCAG
ATCAATGTCAGACCGCTCACCTGGCCTATAAGTCAGGGGATGAACTTCAAAGGGGTCTACAACATCTACTCAGGTCAGCT
GAACCTGTTTGAATCCGATCAGACAAGAATCACTGAAAACTTTATAGAACTTCAGGATATCAACAGCCCTGAACTCGATC
GCTGGATACCGTCTTCATTTACGAAACAGCTTCGGGAGGATGTGGAACTTATCGAAGGTGTCTATGACCCGTTCACCGTA
GAAAACTATCGAAACGGTTCCCTTGCCCCGGTTTTCTTTGGCAGTGCCGTCAACAATTTCGGTATCAGGGAACTGCTCCA
CACATTCCTCGATATCGCACCCTCACCCAGTGAACGCGAAGCCATGCAACGCAGTGTCAAGGCCTCGGAACCAACCATGA
GCGGCTTTGTTTTCAAGATTCATGCAAATCTTGATCCAAACCATCGCGACCGTACTGCATTTTTCAAGATCTGCTCGGGC
ACATTCCAGCGCAACACATTCTATTTCCACACCAGGCTGAAAAAGAAACTGCGCTTTGCAAGCCCTACACAGTTCATGGC
CAACGAAAAAAACCTGATCGAAGATGCCTGGCCGGGTGATGTGATCGGTCTCTATGACAACGGCAACCTCAAAATCGGCG
ACACGCTCACAGAAGGCGAAGAACTCCAGTTCCGCGGTATTCCGAGCTTCTCTCCCGAGATTTTCAAGGAGGTCATCAAC
CGTGATCCTTTCAAGTCAAAGCAGCTCGATAAGGGTGTGCGTCAGCTGACAGAAGAGGGGGTAGCGCAGCTCTTCATGCA
GCACGGCAACAGAAAAATCATCGGTACAGTGGGAGAACTGCAGTTCGATGTGATACGATTCCGTCTCGAACACGAGTATG
GCGCAAAATGCGACTTCATGCCGCTGCGTTACTGCAAAGCGCTCTGGATTACCAGCCGCGACAAAGATCAGTACGAAGAG
TTTCTCAGAAGAAAATCACCGGTCATCGCTCTCGATAAAGAAGAGCATCCTGTCTTTTTCGCCGAAAGCGAATGGATGCT
CAAAGTCGCGATGGAAAACTATCCCGGCGTCATTTTCCACGAAACCTCCGAGTTTAAAACAAAGCGTTAA

Upstream 100 bases:

>100_bases
CACCCATGAACTTTGTATTTTCAGGGCACCTCCGACTACAGAGGAATACCATCGAACAACTTGAGCTTGTCGTGAAACAA
CTGCACCCGAGACGATAGTT

Downstream 100 bases:

>100_bases
AGCGCACCTTCCCCGGAGGATAGAGCGATAGCAATGCGAGCTCTATCCTCTGGATTTCAGCTTCATGTTTTCAGAACTCA
TGCATACGGCATTGAAAATT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSIEKEIAKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVKQNKIRKTATSDFMEIEKQRGISVATSVMGFEYNGIR
INILDTPGHKDFAEDTYRTLTAVDSVILVVDCVKGVEEQTEKLMEVCRMRKTPVIIFINKMDREGKNPVELLDELEEKLQ
INVRPLTWPISQGMNFKGVYNIYSGQLNLFESDQTRITENFIELQDINSPELDRWIPSSFTKQLREDVELIEGVYDPFTV
ENYRNGSLAPVFFGSAVNNFGIRELLHTFLDIAPSPSEREAMQRSVKASEPTMSGFVFKIHANLDPNHRDRTAFFKICSG
TFQRNTFYFHTRLKKKLRFASPTQFMANEKNLIEDAWPGDVIGLYDNGNLKIGDTLTEGEELQFRGIPSFSPEIFKEVIN
RDPFKSKQLDKGVRQLTEEGVAQLFMQHGNRKIIGTVGELQFDVIRFRLEHEYGAKCDFMPLRYCKALWITSRDKDQYEE
FLRRKSPVIALDKEEHPVFFAESEWMLKVAMENYPGVIFHETSEFKTKR

Sequences:

>Translated_529_residues
MSIEKEIAKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVKQNKIRKTATSDFMEIEKQRGISVATSVMGFEYNGIR
INILDTPGHKDFAEDTYRTLTAVDSVILVVDCVKGVEEQTEKLMEVCRMRKTPVIIFINKMDREGKNPVELLDELEEKLQ
INVRPLTWPISQGMNFKGVYNIYSGQLNLFESDQTRITENFIELQDINSPELDRWIPSSFTKQLREDVELIEGVYDPFTV
ENYRNGSLAPVFFGSAVNNFGIRELLHTFLDIAPSPSEREAMQRSVKASEPTMSGFVFKIHANLDPNHRDRTAFFKICSG
TFQRNTFYFHTRLKKKLRFASPTQFMANEKNLIEDAWPGDVIGLYDNGNLKIGDTLTEGEELQFRGIPSFSPEIFKEVIN
RDPFKSKQLDKGVRQLTEEGVAQLFMQHGNRKIIGTVGELQFDVIRFRLEHEYGAKCDFMPLRYCKALWITSRDKDQYEE
FLRRKSPVIALDKEEHPVFFAESEWMLKVAMENYPGVIFHETSEFKTKR
>Mature_528_residues
SIEKEIAKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVKQNKIRKTATSDFMEIEKQRGISVATSVMGFEYNGIRI
NILDTPGHKDFAEDTYRTLTAVDSVILVVDCVKGVEEQTEKLMEVCRMRKTPVIIFINKMDREGKNPVELLDELEEKLQI
NVRPLTWPISQGMNFKGVYNIYSGQLNLFESDQTRITENFIELQDINSPELDRWIPSSFTKQLREDVELIEGVYDPFTVE
NYRNGSLAPVFFGSAVNNFGIRELLHTFLDIAPSPSEREAMQRSVKASEPTMSGFVFKIHANLDPNHRDRTAFFKICSGT
FQRNTFYFHTRLKKKLRFASPTQFMANEKNLIEDAWPGDVIGLYDNGNLKIGDTLTEGEELQFRGIPSFSPEIFKEVINR
DPFKSKQLDKGVRQLTEEGVAQLFMQHGNRKIIGTVGELQFDVIRFRLEHEYGAKCDFMPLRYCKALWITSRDKDQYEEF
LRRKSPVIALDKEEHPVFFAESEWMLKVAMENYPGVIFHETSEFKTKR

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=490, Percent_Identity=27.7551020408163, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI19923640, Length=577, Percent_Identity=26.5164644714038, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI25306283, Length=343, Percent_Identity=30.6122448979592, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI25306287, Length=299, Percent_Identity=32.1070234113712, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI157426893, Length=155, Percent_Identity=32.258064516129, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4503483, Length=158, Percent_Identity=29.746835443038, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=31.2925170068027, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=47.0249520153551, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI1789738, Length=497, Percent_Identity=27.364185110664, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI48994988, Length=179, Percent_Identity=34.0782122905028, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=30.5732484076433, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=491, Percent_Identity=25.4582484725051, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=181, Percent_Identity=35.3591160220994, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=154, Percent_Identity=29.2207792207792, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=33.974358974359, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=30.5970149253731, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=30.5970149253731, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=24.4258872651357, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322359, Length=514, Percent_Identity=25.875486381323, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=27.2151898734177, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=27.2151898734177, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=29.7101449275362, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582462, Length=486, Percent_Identity=27.1604938271605, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI221458488, Length=504, Percent_Identity=26.1904761904762, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI78706572, Length=150, Percent_Identity=34.6666666666667, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=30.0653594771242, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=30.0653594771242, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=30.0653594771242, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60908; Mature: 60777

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEKEIAKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVKQNKIRKTATSDFMEIE
CCCHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHH
KQRGISVATSVMGFEYNGIRINILDTPGHKDFAEDTYRTLTAVDSVILVVDCVKGVEEQT
HHCCCHHHHHHHCEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKLMEVCRMRKTPVIIFINKMDREGKNPVELLDELEEKLQINVRPLTWPISQGMNFKGVY
HHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEEECEEEEEECCCCCCCCEEE
NIYSGQLNLFESDQTRITENFIELQDINSPELDRWIPSSFTKQLREDVELIEGVYDPFTV
EEECCEEEEEECCHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEE
ENYRNGSLAPVFFGSAVNNFGIRELLHTFLDIAPSPSEREAMQRSVKASEPTMSGFVFKI
CCCCCCCCCEEEECCHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEE
HANLDPNHRDRTAFFKICSGTFQRNTFYFHTRLKKKLRFASPTQFMANEKNLIEDAWPGD
ECCCCCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCHHHHCCCCHHHHCCCCCC
VIGLYDNGNLKIGDTLTEGEELQFRGIPSFSPEIFKEVINRDPFKSKQLDKGVRQLTEEG
EEEEEECCCEEECCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VAQLFMQHGNRKIIGTVGELQFDVIRFRLEHEYGAKCDFMPLRYCKALWITSRDKDQYEE
HHHHHHHCCCCEEEEEHHHHHEEHHHEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
FLRRKSPVIALDKEEHPVFFAESEWMLKVAMENYPGVIFHETSEFKTKR
HHHHCCCEEEECCCCCCEEEECCCEEEEEECCCCCCEEEECCHHCCCCC
>Mature Secondary Structure 
SIEKEIAKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVKQNKIRKTATSDFMEIE
CCHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHH
KQRGISVATSVMGFEYNGIRINILDTPGHKDFAEDTYRTLTAVDSVILVVDCVKGVEEQT
HHCCCHHHHHHHCEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKLMEVCRMRKTPVIIFINKMDREGKNPVELLDELEEKLQINVRPLTWPISQGMNFKGVY
HHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEEECEEEEEECCCCCCCCEEE
NIYSGQLNLFESDQTRITENFIELQDINSPELDRWIPSSFTKQLREDVELIEGVYDPFTV
EEECCEEEEEECCHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEE
ENYRNGSLAPVFFGSAVNNFGIRELLHTFLDIAPSPSEREAMQRSVKASEPTMSGFVFKI
CCCCCCCCCEEEECCHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEE
HANLDPNHRDRTAFFKICSGTFQRNTFYFHTRLKKKLRFASPTQFMANEKNLIEDAWPGD
ECCCCCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCHHHHCCCCHHHHCCCCCC
VIGLYDNGNLKIGDTLTEGEELQFRGIPSFSPEIFKEVINRDPFKSKQLDKGVRQLTEEG
EEEEEECCCEEECCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VAQLFMQHGNRKIIGTVGELQFDVIRFRLEHEYGAKCDFMPLRYCKALWITSRDKDQYEE
HHHHHHHCCCCEEEEEHHHHHEEHHHEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
FLRRKSPVIALDKEEHPVFFAESEWMLKVAMENYPGVIFHETSEFKTKR
HHHHCCCEEEECCCCCCEEEECCCEEEEEECCCCCCEEEECCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA