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The map label for this gene is ksgA

Identifier: 194333711

GI number: 194333711

Start: 961158

End: 961943

Strand: Reverse

Name: ksgA

Synonym: Paes_0880

Alternate gene names: 194333711

Gene position: 961943-961158 (Counterclockwise)

Preceding gene: 194333728

Following gene: 194333710

Centisome position: 38.28

GC content: 48.09

Gene sequence:

>786_bases
ATGACAAAAGTTCAATATAAGCATACAGAAATAGCGGTAAAAAAAAAACTGGGTCAAAACTTTCTGACCGACCGAAACAC
CATCAGAAAAATTGTCCAGTCAGCTGAAATCCAACCTGGCGACCATGTCGTTGAAATCGGACCCGGCTTCGGTGCGCTGA
CAAGCGCCATTCTGGAAGTATGCCCTTCGTTCACTGCCGTTGAAAAAGACCGTAAACTCGCAGACTTTATCCGCGAAGAG
TATCCTTCGGTCAACCTTGTTGAATCAGACATCCTTGAGGTGGACTTCGCGAAACTGGCAGAGGAGGGTCCTGTCAAAGT
CATGGGAAACATCCCGTATTCCATCACCAGCCCGATCCTCTTCAAACTGCTCGAAAACCGCAGTCACATTATCTCTGAAA
CCCTGATGATGCAACATGAAGTCGCCCTCAGGCTTGCGGCAAACCCCGGCACAAAAGAGTACGGAATACTTGCTGTTCAG
CTGCAGACCTTCTGCGACGTACGCTATCTGTTCAAAGTCGGAAAAAAAGTGTTTCGCCCCCGGCCGGAGGTCGATAGTGC
GGTCGTCCGGATTGTACCAAAAAAAACCCCACTTGATGATTGTGAGACGGAATTCAGAAGATTTGTACGACTTGCTTTTC
AGCAGAGGAGAAAAACACTGCAGAACAATCTGAAGCAGCACTACGACATTTCACAACTCAGTGCATCTGAACTGCAAAGA
CGGGCCGAATCCTTCACGATAACGGAATTCGAGATGCTTTTCAGCAGGATCCGACCGTTGCAGTAA

Upstream 100 bases:

>100_bases
AGTTTCGGCAACAGTGTTTTGTTCTCCCATTAATCCCAGAATAACTTAAATTGTAAAAACTACTTCCACACTCTTATCTT
TCTCGGCAAAAAGCATCGAA

Downstream 100 bases:

>100_bases
ACACAGATCGCACCCATTTTTATCATTCGTGAACCAGACGTTGCATATCACGCTCTCTCATATAATTCCATTTAACGCTC
TCCATCCATGACTCCTCAGT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTKVQYKHTEIAVKKKLGQNFLTDRNTIRKIVQSAEIQPGDHVVEIGPGFGALTSAILEVCPSFTAVEKDRKLADFIREE
YPSVNLVESDILEVDFAKLAEEGPVKVMGNIPYSITSPILFKLLENRSHIISETLMMQHEVALRLAANPGTKEYGILAVQ
LQTFCDVRYLFKVGKKVFRPRPEVDSAVVRIVPKKTPLDDCETEFRRFVRLAFQQRRKTLQNNLKQHYDISQLSASELQR
RAESFTITEFEMLFSRIRPLQ

Sequences:

>Translated_261_residues
MTKVQYKHTEIAVKKKLGQNFLTDRNTIRKIVQSAEIQPGDHVVEIGPGFGALTSAILEVCPSFTAVEKDRKLADFIREE
YPSVNLVESDILEVDFAKLAEEGPVKVMGNIPYSITSPILFKLLENRSHIISETLMMQHEVALRLAANPGTKEYGILAVQ
LQTFCDVRYLFKVGKKVFRPRPEVDSAVVRIVPKKTPLDDCETEFRRFVRLAFQQRRKTLQNNLKQHYDISQLSASELQR
RAESFTITEFEMLFSRIRPLQ
>Mature_260_residues
TKVQYKHTEIAVKKKLGQNFLTDRNTIRKIVQSAEIQPGDHVVEIGPGFGALTSAILEVCPSFTAVEKDRKLADFIREEY
PSVNLVESDILEVDFAKLAEEGPVKVMGNIPYSITSPILFKLLENRSHIISETLMMQHEVALRLAANPGTKEYGILAVQL
QTFCDVRYLFKVGKKVFRPRPEVDSAVVRIVPKKTPLDDCETEFRRFVRLAFQQRRKTLQNNLKQHYDISQLSASELQRR
AESFTITEFEMLFSRIRPLQ

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=215, Percent_Identity=35.8139534883721, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI156415992, Length=273, Percent_Identity=32.2344322344322, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1786236, Length=253, Percent_Identity=36.3636363636364, Blast_Score=159, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25146882, Length=206, Percent_Identity=36.8932038834951, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25141369, Length=245, Percent_Identity=32.2448979591837, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=210, Percent_Identity=34.7619047619048, Blast_Score=113, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21358017, Length=223, Percent_Identity=36.322869955157, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21357273, Length=265, Percent_Identity=29.811320754717, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PROA2 (B4S787)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015571.1
- GeneID:   6458537
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_0880
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 29912; Mature: 29781

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 20-20 BINDING 22-22 BINDING 47-47 BINDING 68-68 BINDING 90-90 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVQYKHTEIAVKKKLGQNFLTDRNTIRKIVQSAEIQPGDHVVEIGPGFGALTSAILEV
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHH
CPSFTAVEKDRKLADFIREEYPSVNLVESDILEVDFAKLAEEGPVKVMGNIPYSITSPIL
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHH
FKLLENRSHIISETLMMQHEVALRLAANPGTKEYGILAVQLQTFCDVRYLFKVGKKVFRP
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
RPEVDSAVVRIVPKKTPLDDCETEFRRFVRLAFQQRRKTLQNNLKQHYDISQLSASELQR
CCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RAESFTITEFEMLFSRIRPLQ
HHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKVQYKHTEIAVKKKLGQNFLTDRNTIRKIVQSAEIQPGDHVVEIGPGFGALTSAILEV
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHH
CPSFTAVEKDRKLADFIREEYPSVNLVESDILEVDFAKLAEEGPVKVMGNIPYSITSPIL
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHH
FKLLENRSHIISETLMMQHEVALRLAANPGTKEYGILAVQLQTFCDVRYLFKVGKKVFRP
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
RPEVDSAVVRIVPKKTPLDDCETEFRRFVRLAFQQRRKTLQNNLKQHYDISQLSASELQR
CCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RAESFTITEFEMLFSRIRPLQ
HHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA