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The map label for this gene is macA [H]

Identifier: 193214360

GI number: 193214360

Start: 754356

End: 755699

Strand: Direct

Name: macA [H]

Synonym: Ctha_0642

Alternate gene names: 193214360

Gene position: 754356-755699 (Clockwise)

Preceding gene: 193214359

Following gene: 193214373

Centisome position: 22.9

GC content: 47.69

Gene sequence:

>1344_bases
ATGCAAACACCGCTAAGCAAGGGAACCCACCCAAAGAAAGCCAAAAAAAAATTTACTACTTTCCACTACATCCTTTTTGT
AGCGGGGCTTATCGGATTAGGCGTTGCTGCCAAATTCATATTTTTCAACAACAAAGACGAAGTCATTGAGGTTAGCACCG
AAAAAGCCATTGTCAAAAACATCACGCAGATCGTTTCTGCTACGGGAAAAATTCAGCCCGAAACGGAAGTCGTTATTTCG
CCAGACGTTTCCGGTGAAATTATTGCCTTACCGGTGAAAGAAGGCCAGCATGTCGAAAAAGGCGCGCTGCTATTTCGAAT
TAAGCCCGAATTGCTTCAAGCTGAAGTCGAGCAACAGGAAGCATCTTTGAGCGCAGCGAAAGCCAAAAGCCTTCAGTCGA
AAGCGCAACTGTTGCTCGCCGACGATGAGTTTCGCCGAACAAAAGAGCTGTTTGAAAAAAAGCTGGTTTCGGAATCTGAT
TTTATTACCGCCAAAACCAACGCCGACGTCGCCAAGGCGACTTATGAAGCCTCCCTTTTTGAAATCAATCGCGTTGAAAG
CCTTTTAAAACAAAGTCGCGACCAGCTCTCGCGAACAACCGTTTATGCGCCGATGAGCGGCACCATTACGGTTCTCAACA
ACAAACTTGGCGAACGCGTGGTTGCCACCAGTCAATACACCGGAACGGAAGTCCTGAAAATTGCGGACTTAAGCAGCATG
GAAATCATGGTGGAAGTCAATGAGAACGACATCGTCAATGTGAAGCTGGCCGATACGTCGCGCATTTATGTGGATGCGTA
CCCCGACCGAACCTTCATGGGCACGGTCACCGAAATTGCCAGCACAGCTGTAACCACCTCTGAAGGCACGCAGGAAGAAG
TCACCAACTTTGACGTGACCATTCGCATTTTAGACCACGAGCAGCTTCTGCGCCCGGGCATGAGCGCCACAGCCGATATT
GAAACGGCCACCGTTCACGATGCGATTGCCGTACCCATTCAAAGCGTTACGGTTCGTTCGCCGAAAGCTGAACCTGAAAA
AACAGAGACGAACACCAAACAAAAAGATGAAAACGACACAAAAGTTATCGGTGAAAATGAAGCATCTGCAAGCAAAAAGC
TGCGTCAAGATGAGCTTGTGAAAGTGATTTTTATAAAAAAAGGCGACAAGGCCGAAATGCGCCGGGTTGAAACGGGCATT
GCGGATAATACTCACATTCAAATCTTAGCTGGCATTGAATCCGGTGAAGAAGTGATTTCCGGCAGCTACCGCGCCATTAG
CCGCGACCTTTCAGATGGCTCGCTTGTCAAAATTCCAGAACGGAAGGGAAAAAAGAAGGATTAA

Upstream 100 bases:

>100_bases
TTCCCTGAGTAGAAACGCATTTGTGCCTTTTTTATGTTTCTCTCTTTCTATTACGTTTAGGAAAAGAGAGAAACATTATT
AGGCAAAAAAAATATCAACT

Downstream 100 bases:

>100_bases
ACTCATGAGCTCGCTGCCAAATTAGCGGCAAAAGCCTCATTTCGTTTGATCAAAAATAAAAAACCGGCAGTTTTGAAACT
GCCGGCTGACAGAAGGGAGA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MQTPLSKGTHPKKAKKKFTTFHYILFVAGLIGLGVAAKFIFFNNKDEVIEVSTEKAIVKNITQIVSATGKIQPETEVVIS
PDVSGEIIALPVKEGQHVEKGALLFRIKPELLQAEVEQQEASLSAAKAKSLQSKAQLLLADDEFRRTKELFEKKLVSESD
FITAKTNADVAKATYEASLFEINRVESLLKQSRDQLSRTTVYAPMSGTITVLNNKLGERVVATSQYTGTEVLKIADLSSM
EIMVEVNENDIVNVKLADTSRIYVDAYPDRTFMGTVTEIASTAVTTSEGTQEEVTNFDVTIRILDHEQLLRPGMSATADI
ETATVHDAIAVPIQSVTVRSPKAEPEKTETNTKQKDENDTKVIGENEASASKKLRQDELVKVIFIKKGDKAEMRRVETGI
ADNTHIQILAGIESGEEVISGSYRAISRDLSDGSLVKIPERKGKKKD

Sequences:

>Translated_447_residues
MQTPLSKGTHPKKAKKKFTTFHYILFVAGLIGLGVAAKFIFFNNKDEVIEVSTEKAIVKNITQIVSATGKIQPETEVVIS
PDVSGEIIALPVKEGQHVEKGALLFRIKPELLQAEVEQQEASLSAAKAKSLQSKAQLLLADDEFRRTKELFEKKLVSESD
FITAKTNADVAKATYEASLFEINRVESLLKQSRDQLSRTTVYAPMSGTITVLNNKLGERVVATSQYTGTEVLKIADLSSM
EIMVEVNENDIVNVKLADTSRIYVDAYPDRTFMGTVTEIASTAVTTSEGTQEEVTNFDVTIRILDHEQLLRPGMSATADI
ETATVHDAIAVPIQSVTVRSPKAEPEKTETNTKQKDENDTKVIGENEASASKKLRQDELVKVIFIKKGDKAEMRRVETGI
ADNTHIQILAGIESGEEVISGSYRAISRDLSDGSLVKIPERKGKKKD
>Mature_447_residues
MQTPLSKGTHPKKAKKKFTTFHYILFVAGLIGLGVAAKFIFFNNKDEVIEVSTEKAIVKNITQIVSATGKIQPETEVVIS
PDVSGEIIALPVKEGQHVEKGALLFRIKPELLQAEVEQQEASLSAAKAKSLQSKAQLLLADDEFRRTKELFEKKLVSESD
FITAKTNADVAKATYEASLFEINRVESLLKQSRDQLSRTTVYAPMSGTITVLNNKLGERVVATSQYTGTEVLKIADLSSM
EIMVEVNENDIVNVKLADTSRIYVDAYPDRTFMGTVTEIASTAVTTSEGTQEEVTNFDVTIRILDHEQLLRPGMSATADI
ETATVHDAIAVPIQSVTVRSPKAEPEKTETNTKQKDENDTKVIGENEASASKKLRQDELVKVIFIKKGDKAEMRRVETGI
ADNTHIQILAGIESGEEVISGSYRAISRDLSDGSLVKIPERKGKKKD

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=413, Percent_Identity=25.4237288135593, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87081951, Length=214, Percent_Identity=28.5046728971963, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790519, Length=239, Percent_Identity=25.9414225941423, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 49157; Mature: 49157

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTPLSKGTHPKKAKKKFTTFHYILFVAGLIGLGVAAKFIFFNNKDEVIEVSTEKAIVKN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEECHHHHHHHH
ITQIVSATGKIQPETEVVISPDVSGEIIALPVKEGQHVEKGALLFRIKPELLQAEVEQQE
HHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHH
ASLSAAKAKSLQSKAQLLLADDEFRRTKELFEKKLVSESDFITAKTNADVAKATYEASLF
HHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
EINRVESLLKQSRDQLSRTTVYAPMSGTITVLNNKLGERVVATSQYTGTEVLKIADLSSM
HHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCEEEEECCCCCCEEEEEECCCCE
EIMVEVNENDIVNVKLADTSRIYVDAYPDRTFMGTVTEIASTAVTTSEGTQEEVTNFDVT
EEEEEECCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHEECCCCCHHHHCCEEEE
IRILDHEQLLRPGMSATADIETATVHDAIAVPIQSVTVRSPKAEPEKTETNTKQKDENDT
EEEECHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
KVIGENEASASKKLRQDELVKVIFIKKGDKAEMRRVETGIADNTHIQILAGIESGEEVIS
EEECCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHC
GSYRAISRDLSDGSLVKIPERKGKKKD
CCHHHHHCCCCCCCEEECCHHCCCCCC
>Mature Secondary Structure
MQTPLSKGTHPKKAKKKFTTFHYILFVAGLIGLGVAAKFIFFNNKDEVIEVSTEKAIVKN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEECHHHHHHHH
ITQIVSATGKIQPETEVVISPDVSGEIIALPVKEGQHVEKGALLFRIKPELLQAEVEQQE
HHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHH
ASLSAAKAKSLQSKAQLLLADDEFRRTKELFEKKLVSESDFITAKTNADVAKATYEASLF
HHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
EINRVESLLKQSRDQLSRTTVYAPMSGTITVLNNKLGERVVATSQYTGTEVLKIADLSSM
HHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCEEEEECCCCCCEEEEEECCCCE
EIMVEVNENDIVNVKLADTSRIYVDAYPDRTFMGTVTEIASTAVTTSEGTQEEVTNFDVT
EEEEEECCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHEECCCCCHHHHCCEEEE
IRILDHEQLLRPGMSATADIETATVHDAIAVPIQSVTVRSPKAEPEKTETNTKQKDENDT
EEEECHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
KVIGENEASASKKLRQDELVKVIFIKKGDKAEMRRVETGIADNTHIQILAGIESGEEVIS
EEECCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHC
GSYRAISRDLSDGSLVKIPERKGKKKD
CCHHHHHCCCCCCCEEECCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]