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The map label for this gene is ccsA [H]

Identifier: 193214331

GI number: 193214331

Start: 720446

End: 721741

Strand: Direct

Name: ccsA [H]

Synonym: Ctha_0613

Alternate gene names: 193214331

Gene position: 720446-721741 (Clockwise)

Preceding gene: 193214329

Following gene: 193214333

Centisome position: 21.88

GC content: 46.84

Gene sequence:

>1296_bases
ATGGGTAAGAAAACCGTCACCATAACAAACAACGCCACCGGACAAAGTGAAGAGTTTCCCTTGCTGAATGGAACGCATGG
CCCCGATGTGTTCGACATTCGCCAGCTGTACAAAAAAATGAATTTGTTTACCTACGATCCGGGTTACAAATCCACCGCAA
GCTGCGAAAGCAAAATCACTTATATCGATGGCGATGAGGGCATTTTGCTCTATCGCGGCTACCCCATCGAGCAACTTGCA
GAGAAGAGCAATTTTCTTGAAGTCTGCTACTTGATTTTTTATGGCGAGCTCCCAACGCAAGAGCAATACGAAGAGTTCGT
TTATGTGATCAAGCACCATTCTATGGTTCATGAAGGATTTAGAGATTTTATCAATGGATTTCGCCGCGACGCACATCCGA
TGGCGATTATGGTCGGCATGGTTGGCGCAATGAGCGCGTTCTACCACGATTCCACCGATATTACCAACCCCAGCCACCAA
GAAATTTCAGCGCACCGCTTGTTAGCTAAAATTCCTACCATTGCGGCCATGTCCTATAAATACACGATAGGGCAACCCTT
TATTTATCCAAGAAATGACTTGGGCTATGCAGAAAATTTCTTGCACATGATGTATGCCGTTCCTGCAGAGCCGTATCACA
TTGACCCACTCTATGCAAAAGCGTTGGATTTGATTTTGATCCTTCATGCCGACCACGAACAAAATGCCTCTGCCTCTACG
GTGCGTTTGGCGGGTTCTTCAGGTGCAAACCCATTTGCAGCGGCTTCAGCTGGCATTGGCTGTTTGTGGGGCGCGGCTCA
TGGCGGTGCAAACGAAGCGGTTATCCGAATGTTGGAAAAGATCGGCTCAGCCAATCACATTCCTGAATTTATTGCCAGGG
CGAAGGACAAAGATGATTCATTTCGGTTGATGGGATTCGGACATCGTGTCTATAAGCATTATGATCCGCGCGCGAAAATT
ATCCGCAAAATGGCCATCGACATTATCAATAAGACCAGCGGAAACGACCCGCTTTTGAACATCGCTATGAAGCTCGAAGA
AATTGCCCTAACCGATGAATATTTCATCGAGAAGAAGCTTTATCCAAATGTGGATTTTTATTCCGGCATTATTTATAAAG
CCCTGGGCATCCCAACCAGCATGTTTACCGTCATTTTTGTGATGGGACGCATGATCGGCTGGATTGCACAATGGCGCGAA
ATGATCCAAGATCCAGAACGCGTGATTGGACGGCCTCGCCAAAACTATATCGGCCATGCCTCGCGCGACTTTGTGCCAAT
TGATGAACGAAAATGA

Upstream 100 bases:

>100_bases
ACGTCGACTTTTTTTAAAACATCATTTCTTTTTTTTAGATGGCGCAGCATCCATAGCATGCGCTTTTTTCAAACCGCCAA
GACATTTCGGAGGGTATCAT

Downstream 100 bases:

>100_bases
ATTTGAGTAGGTGAGGAGAAAAACCCAACGGTTTTCAAGGTTTTCTCCTCATTTCATCGGATAAGTAACCGGACAGAAAT
AGATAAGATAAAAAAACAGC

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGKKTVTITNNATGQSEEFPLLNGTHGPDVFDIRQLYKKMNLFTYDPGYKSTASCESKITYIDGDEGILLYRGYPIEQLA
EKSNFLEVCYLIFYGELPTQEQYEEFVYVIKHHSMVHEGFRDFINGFRRDAHPMAIMVGMVGAMSAFYHDSTDITNPSHQ
EISAHRLLAKIPTIAAMSYKYTIGQPFIYPRNDLGYAENFLHMMYAVPAEPYHIDPLYAKALDLILILHADHEQNASAST
VRLAGSSGANPFAAASAGIGCLWGAAHGGANEAVIRMLEKIGSANHIPEFIARAKDKDDSFRLMGFGHRVYKHYDPRAKI
IRKMAIDIINKTSGNDPLLNIAMKLEEIALTDEYFIEKKLYPNVDFYSGIIYKALGIPTSMFTVIFVMGRMIGWIAQWRE
MIQDPERVIGRPRQNYIGHASRDFVPIDERK

Sequences:

>Translated_431_residues
MGKKTVTITNNATGQSEEFPLLNGTHGPDVFDIRQLYKKMNLFTYDPGYKSTASCESKITYIDGDEGILLYRGYPIEQLA
EKSNFLEVCYLIFYGELPTQEQYEEFVYVIKHHSMVHEGFRDFINGFRRDAHPMAIMVGMVGAMSAFYHDSTDITNPSHQ
EISAHRLLAKIPTIAAMSYKYTIGQPFIYPRNDLGYAENFLHMMYAVPAEPYHIDPLYAKALDLILILHADHEQNASAST
VRLAGSSGANPFAAASAGIGCLWGAAHGGANEAVIRMLEKIGSANHIPEFIARAKDKDDSFRLMGFGHRVYKHYDPRAKI
IRKMAIDIINKTSGNDPLLNIAMKLEEIALTDEYFIEKKLYPNVDFYSGIIYKALGIPTSMFTVIFVMGRMIGWIAQWRE
MIQDPERVIGRPRQNYIGHASRDFVPIDERK
>Mature_430_residues
GKKTVTITNNATGQSEEFPLLNGTHGPDVFDIRQLYKKMNLFTYDPGYKSTASCESKITYIDGDEGILLYRGYPIEQLAE
KSNFLEVCYLIFYGELPTQEQYEEFVYVIKHHSMVHEGFRDFINGFRRDAHPMAIMVGMVGAMSAFYHDSTDITNPSHQE
ISAHRLLAKIPTIAAMSYKYTIGQPFIYPRNDLGYAENFLHMMYAVPAEPYHIDPLYAKALDLILILHADHEQNASASTV
RLAGSSGANPFAAASAGIGCLWGAAHGGANEAVIRMLEKIGSANHIPEFIARAKDKDDSFRLMGFGHRVYKHYDPRAKII
RKMAIDIINKTSGNDPLLNIAMKLEEIALTDEYFIEKKLYPNVDFYSGIIYKALGIPTSMFTVIFVMGRMIGWIAQWREM
IQDPERVIGRPRQNYIGHASRDFVPIDERK

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=388, Percent_Identity=27.0618556701031, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786939, Length=407, Percent_Identity=64.6191646191646, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1786527, Length=366, Percent_Identity=31.9672131147541, Blast_Score=171, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17555174, Length=413, Percent_Identity=27.1186440677966, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319850, Length=358, Percent_Identity=28.4916201117318, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=379, Percent_Identity=25.8575197889182, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6325257, Length=423, Percent_Identity=25.2955082742317, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24640124, Length=400, Percent_Identity=25.75, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24640126, Length=400, Percent_Identity=25.75, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356863, Length=395, Percent_Identity=26.8354430379747, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48750; Mature: 48618

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKTVTITNNATGQSEEFPLLNGTHGPDVFDIRQLYKKMNLFTYDPGYKSTASCESKIT
CCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEEE
YIDGDEGILLYRGYPIEQLAEKSNFLEVCYLIFYGELPTQEQYEEFVYVIKHHSMVHEGF
EEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
RDFINGFRRDAHPMAIMVGMVGAMSAFYHDSTDITNPSHQEISAHRLLAKIPTIAAMSYK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHEEEE
YTIGQPFIYPRNDLGYAENFLHMMYAVPAEPYHIDPLYAKALDLILILHADHEQNASAST
EECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEEECCCCCCCCCCE
VRLAGSSGANPFAAASAGIGCLWGAAHGGANEAVIRMLEKIGSANHIPEFIARAKDKDDS
EEEECCCCCCCHHHHHCCCHHEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCC
FRLMGFGHRVYKHYDPRAKIIRKMAIDIINKTSGNDPLLNIAMKLEEIALTDEYFIEKKL
EEEEECCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
YPNVDFYSGIIYKALGIPTSMFTVIFVMGRMIGWIAQWREMIQDPERVIGRPRQNYIGHA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHCCCC
SRDFVPIDERK
CCCCCCCCCCC
>Mature Secondary Structure 
GKKTVTITNNATGQSEEFPLLNGTHGPDVFDIRQLYKKMNLFTYDPGYKSTASCESKIT
CCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEEE
YIDGDEGILLYRGYPIEQLAEKSNFLEVCYLIFYGELPTQEQYEEFVYVIKHHSMVHEGF
EEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
RDFINGFRRDAHPMAIMVGMVGAMSAFYHDSTDITNPSHQEISAHRLLAKIPTIAAMSYK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHEEEE
YTIGQPFIYPRNDLGYAENFLHMMYAVPAEPYHIDPLYAKALDLILILHADHEQNASAST
EECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEEECCCCCCCCCCE
VRLAGSSGANPFAAASAGIGCLWGAAHGGANEAVIRMLEKIGSANHIPEFIARAKDKDDS
EEEECCCCCCCHHHHHCCCHHEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCC
FRLMGFGHRVYKHYDPRAKIIRKMAIDIINKTSGNDPLLNIAMKLEEIALTDEYFIEKKL
EEEEECCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
YPNVDFYSGIIYKALGIPTSMFTVIFVMGRMIGWIAQWREMIQDPERVIGRPRQNYIGHA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHCCCC
SRDFVPIDERK
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]