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The map label for this gene is nth [H]

Identifier: 193214276

GI number: 193214276

Start: 655350

End: 655991

Strand: Direct

Name: nth [H]

Synonym: Ctha_0557

Alternate gene names: 193214276

Gene position: 655350-655991 (Clockwise)

Preceding gene: 193214275

Following gene: 193214277

Centisome position: 19.9

GC content: 44.86

Gene sequence:

>642_bases
ATGACACGAGACGAAAAACTTGAACTGGTAGCTAAAATTCTTGGGGCAAAACATCCTGCACCAAAAACAGAACTCAACTA
CGAAACGCCTTTTCAACTTTTGGTCGCAACCATTTTAGCCGCACAATGCACCGACAAACGCGTTAATCTCGTAACCGCAG
CACTTTTCCAGCGCTATCCTGATGCAAAATCTATGAGCGAGCTTTCGTTTGAAGCACTTCGCGAGGAAATCAAATCCATT
AATTTTTTGAACAATAAGGCCAAAAATATTCTCGACTCGAGCAAGGCGCTTGTTGAAAAATACAACGGAGAAGTACCTGA
TACGCTCGATGCCTTAACCGCATTGCCTGGCGTTGGTAGAAAAACAGCCCACGTGGTGATGAGCAACGCATTTGGCAAAC
CGGTATTGGCAGTCGATACACATGTGCATCGCGTTGCCAATCGGCTTGGGTTGGCCAATTCCAAAAATGTGCGCGACACA
GAAAATCAGCTCATGGAGATTTTGCCGGAATCCCTCGTCAGCGATTTTCACCATTACCTGATTTTGCATGGACGCTACAC
TTGCAAAGCGCGCTCACCTCAATGTATGAATTGCGAATTAACACATATTTGCAACTATTTCCACTCAAAAACGAACGATT
AG

Upstream 100 bases:

>100_bases
TTGGAAGATTAACGGCCATTGAACTTCCATCGCGAAAAATTACACAGGTTGAAAATCAAGATTTACCTTAAACCTGTCTC
TAAAATTTTTATCGAACAAA

Downstream 100 bases:

>100_bases
TTTGGGTGTGTTTTTTGACTAAGTTTAGAGTTTTTCTAACTTTCTCATTCTTTTTCAAGTCCTGGATAATTGTCGTTTTC
TTGTTTAGTAACGCCTGTTT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTRDEKLELVAKILGAKHPAPKTELNYETPFQLLVATILAAQCTDKRVNLVTAALFQRYPDAKSMSELSFEALREEIKSI
NFLNNKAKNILDSSKALVEKYNGEVPDTLDALTALPGVGRKTAHVVMSNAFGKPVLAVDTHVHRVANRLGLANSKNVRDT
ENQLMEILPESLVSDFHHYLILHGRYTCKARSPQCMNCELTHICNYFHSKTND

Sequences:

>Translated_213_residues
MTRDEKLELVAKILGAKHPAPKTELNYETPFQLLVATILAAQCTDKRVNLVTAALFQRYPDAKSMSELSFEALREEIKSI
NFLNNKAKNILDSSKALVEKYNGEVPDTLDALTALPGVGRKTAHVVMSNAFGKPVLAVDTHVHRVANRLGLANSKNVRDT
ENQLMEILPESLVSDFHHYLILHGRYTCKARSPQCMNCELTHICNYFHSKTND
>Mature_212_residues
TRDEKLELVAKILGAKHPAPKTELNYETPFQLLVATILAAQCTDKRVNLVTAALFQRYPDAKSMSELSFEALREEIKSIN
FLNNKAKNILDSSKALVEKYNGEVPDTLDALTALPGVGRKTAHVVMSNAFGKPVLAVDTHVHRVANRLGLANSKNVRDTE
NQLMEILPESLVSDFHHYLILHGRYTCKARSPQCMNCELTHICNYFHSKTND

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=182, Percent_Identity=34.6153846153846, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1787920, Length=206, Percent_Identity=47.0873786407767, Blast_Score=203, Evalue=7e-54,
Organism=Escherichia coli, GI1789331, Length=182, Percent_Identity=22.5274725274725, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=35.7541899441341, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324530, Length=193, Percent_Identity=25.3886010362694, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319304, Length=176, Percent_Identity=29.5454545454545, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI45550361, Length=186, Percent_Identity=33.8709677419355, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23847; Mature: 23715

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDEKLELVAKILGAKHPAPKTELNYETPFQLLVATILAAQCTDKRVNLVTAALFQRYP
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
DAKSMSELSFEALREEIKSINFLNNKAKNILDSSKALVEKYNGEVPDTLDALTALPGVGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
KTAHVVMSNAFGKPVLAVDTHVHRVANRLGLANSKNVRDTENQLMEILPESLVSDFHHYL
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILHGRYTCKARSPQCMNCELTHICNYFHSKTND
HHCCCEEECCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRDEKLELVAKILGAKHPAPKTELNYETPFQLLVATILAAQCTDKRVNLVTAALFQRYP
CCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
DAKSMSELSFEALREEIKSINFLNNKAKNILDSSKALVEKYNGEVPDTLDALTALPGVGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
KTAHVVMSNAFGKPVLAVDTHVHRVANRLGLANSKNVRDTENQLMEILPESLVSDFHHYL
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILHGRYTCKARSPQCMNCELTHICNYFHSKTND
HHCCCEEECCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]