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The map label for this gene is phoB [H]

Identifier: 193214197

GI number: 193214197

Start: 561091

End: 561774

Strand: Direct

Name: phoB [H]

Synonym: Ctha_0478

Alternate gene names: 193214197

Gene position: 561091-561774 (Clockwise)

Preceding gene: 193214196

Following gene: 193214201

Centisome position: 17.04

GC content: 44.01

Gene sequence:

>684_bases
ATGAATCCGCGAACTATACTTATTGCTGATGATGAATCTCAGTACGTATCGTGGTTACGAACGCGCCTTGAACAAGAGGG
ATATAATATTGTTTGGGCTCGTGATGCAGCTGAAACGCTTGCTCATCTTTCACAAAAGCCTGAAGTTGTCCTTCTCGATT
TAATGAACGACTCCAATGGCTTAGAGCTTTGCCGTCAGCTCCGTGCGTTGCCTGAGGGAAAGTCCGTTCCGATTATCATG
CTTGCCAGCAAAAGTGAAGAATCGGATGAAGTGCTTGGCCTTGAGCTTGGCGCCGATGATTTTTTGAGCAAAACGACAAG
TCCAAAGGTGCTTTCTGCACGTATTAAAAATGTGCTGAAGCGCTATCATGTTAATGCTCGTGAGGAATTGACTCGTCTTA
AAATTGGCGATCTCGAAATCGACCGCAGTTCGCACACCGTGCGCCTCGATGAAAAAGATATTTTCTTGCCTCGTAAGGAA
TTTGAACTGCTTTGGATTTTAGCCACAAACAAAGGCAAAGTTTTTTCTCGTGAAATGTTGCTTCGCCGCGTTTGGGGTGA
AAATATTTATGTGACAGATCGCACGGTCGATGTGCATATTTGCAAAGTTCGTCAACGTCTTGGAAAATTTGGCGAAGATG
CGATTGAAACCATTAAAGGGGTTGGCTATCGCTTAAAGTTTTAA

Upstream 100 bases:

>100_bases
CAGATCAAGAACAACATTTTGTTCTTTCGCGCTATTATTATCTTCTCGTACATTCTGCTCTCTTTCCAAGAAACACCAAC
AACTAATCAACTAAACATAT

Downstream 100 bases:

>100_bases
ATGATTGAATCGTAAATCTTACCGAAATTCTTACCGAAATAGTTTAAAATCAGTCCCGGTAAGAGGAATGGTGAATGCAG
GGCCTTGCGAAGCCAAGAGC

Product: winged helix family two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MNPRTILIADDESQYVSWLRTRLEQEGYNIVWARDAAETLAHLSQKPEVVLLDLMNDSNGLELCRQLRALPEGKSVPIIM
LASKSEESDEVLGLELGADDFLSKTTSPKVLSARIKNVLKRYHVNAREELTRLKIGDLEIDRSSHTVRLDEKDIFLPRKE
FELLWILATNKGKVFSREMLLRRVWGENIYVTDRTVDVHICKVRQRLGKFGEDAIETIKGVGYRLKF

Sequences:

>Translated_227_residues
MNPRTILIADDESQYVSWLRTRLEQEGYNIVWARDAAETLAHLSQKPEVVLLDLMNDSNGLELCRQLRALPEGKSVPIIM
LASKSEESDEVLGLELGADDFLSKTTSPKVLSARIKNVLKRYHVNAREELTRLKIGDLEIDRSSHTVRLDEKDIFLPRKE
FELLWILATNKGKVFSREMLLRRVWGENIYVTDRTVDVHICKVRQRLGKFGEDAIETIKGVGYRLKF
>Mature_227_residues
MNPRTILIADDESQYVSWLRTRLEQEGYNIVWARDAAETLAHLSQKPEVVLLDLMNDSNGLELCRQLRALPEGKSVPIIM
LASKSEESDEVLGLELGADDFLSKTTSPKVLSARIKNVLKRYHVNAREELTRLKIGDLEIDRSSHTVRLDEKDIFLPRKE
FELLWILATNKGKVFSREMLLRRVWGENIYVTDRTVDVHICKVRQRLGKFGEDAIETIKGVGYRLKF

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=38.6666666666667, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1790860, Length=230, Percent_Identity=34.7826086956522, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=37.0044052863436, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI2367329, Length=226, Percent_Identity=35.3982300884956, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=32.4444444444444, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=33.9366515837104, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1790863, Length=235, Percent_Identity=34.0425531914894, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=34.3478260869565, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693140, Length=237, Percent_Identity=32.9113924050633, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789809, Length=230, Percent_Identity=29.1304347826087, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786784, Length=225, Percent_Identity=31.1111111111111, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=29.2792792792793, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1790552, Length=226, Percent_Identity=29.646017699115, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=24.8868778280543, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26137; Mature: 26137

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPRTILIADDESQYVSWLRTRLEQEGYNIVWARDAAETLAHLSQKPEVVLLDLMNDSNG
CCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCH
LELCRQLRALPEGKSVPIIMLASKSEESDEVLGLELGADDFLSKTTSPKVLSARIKNVLK
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEECCHHHHHCCCCCHHHHHHHHHHHH
RYHVNAREELTRLKIGDLEIDRSSHTVRLDEKDIFLPRKEFELLWILATNKGKVFSREML
HHCCCHHHHHHHEEECCEEECCCCCEEEECCCCEECCCCCCEEEEEEECCCCCHHHHHHH
LRRVWGENIYVTDRTVDVHICKVRQRLGKFGEDAIETIKGVGYRLKF
HHHHCCCCEEEECCEEEHHHHHHHHHHCCCCHHHHHHHHCCCEEECC
>Mature Secondary Structure
MNPRTILIADDESQYVSWLRTRLEQEGYNIVWARDAAETLAHLSQKPEVVLLDLMNDSNG
CCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCH
LELCRQLRALPEGKSVPIIMLASKSEESDEVLGLELGADDFLSKTTSPKVLSARIKNVLK
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEECCHHHHHCCCCCHHHHHHHHHHHH
RYHVNAREELTRLKIGDLEIDRSSHTVRLDEKDIFLPRKEFELLWILATNKGKVFSREML
HHCCCHHHHHHHEEECCEEECCCCCEEEECCCCEECCCCCCEEEEEEECCCCCHHHHHHH
LRRVWGENIYVTDRTVDVHICKVRQRLGKFGEDAIETIKGVGYRLKF
HHHHCCCCEEEECCEEEHHHHHHHHHHCCCCHHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]