Click here to switch to the map view.

The map label for this gene is pfkA1 [H]

Identifier: 193213870

GI number: 193213870

Start: 178183

End: 179349

Strand: Direct

Name: pfkA1 [H]

Synonym: Ctha_0151

Alternate gene names: 193213870

Gene position: 178183-179349 (Clockwise)

Preceding gene: 193213865

Following gene: 193213871

Centisome position: 5.41

GC content: 48.41

Gene sequence:

>1167_bases
ATGAAAATCGGTATCCTAACAGGCGGCGGTGATGTTCCCGGCCTAAATCCGTGCATGAAAGCAGTTGTTTATCACGCTAT
TGATGATGGACATGAAATTGTCGGCATTCGGCGTGGATGGGGCGGTTTGTTGAATTTTAACCCAGATGACCCAAAAAGCT
ACGACGAAAACATCATTCCGCTCGATAAGCAAAAAGTGCGCACCATTGATCGAACTGGCGGAACGTTTTTGCATTCAAGT
CGCACGAATCCAAGCAAAGTCAGAGTGAAAGATGTACCTGATTTTCTGCGTGACCCAAAAACAAAGAACACTGACGACGC
CTCGTCCGATATTCTGGATTTCACTCCGCATGTTCTCAAAAACCTTGAAATACTTGGAATCGATACCTTAATCCCTATCG
GTGGCGACGATACATTAAGCTACGGCGAGCGTCTACACCGTGAGGGCGTGTCGGTTGTTGCCATTCCAAAAACCATGGAC
AACGACGTTTTTGGAACAGACTACTGCATTGGCTTCAGCACTGCTGTAACCCGAAGCGTGCAATTTATCCATCAACTTCG
CACCAGCACCGGTTCGCATGAACGCATTGCGGTTATCGAGCTGTTTGGCCGCAATTCCGGCGAAACCAGCCTGATCAGCT
CCTATCTTTCTGGCGTTGATAGAGCCATTATTTCTGAAGTCCCATTTGATCCGGAAATTTTAGCCTCGCTTCTTCTAAAA
GATTATAACGCCAATCCAAGCAGCTACGCGATGGTGACGATTAGCGAAGGCGCATCGATGGTTGGTGGAAAAATTATTGA
GTATGGCGAAGCGGACGCTTATGGCCACCGCAAGTTGGGCGGCATCGGCATGATCACAGGTGAAGCCGTCAAAAAACTAA
CAGGAAAAGGTATTGTCTATCAGCAAGTTGGCTACTTAATGCGCTCAGGCGCACCCGATGCACTCGACTTGATGATTGCC
ATTAACTTCGCCGGCATTGCCATGGGACTTTTGAAAGATAAGGTGAGCGGACGCATGGTAGCTCTTCGCGATGGCACATA
CACGCACATCCCAATTAGCACCATTGCAAGCGGCATCAAACGAGTCGACGTCGACGAGCTGTACGATCCAAAAGAATATC
GCCCGAAAGTCCGCAACGTGCTCGGCAAACCCATGTTTCTTTATTGA

Upstream 100 bases:

>100_bases
GCCTATGGCAGAACATTCTGAAATCGACTATATTGTAGAAGTCCATTGCAAGGGCAAATTCTCCTGTAAAGCAAACCAAC
AGAGAAAGGATCACATCAAC

Downstream 100 bases:

>100_bases
TAGCTTAAAGGCCATTTTACCTGGCGCTTGCTTGACGAAATCAGCATTTTTTGGCATCAAAATCATACATTTTCGTTGAG
TTTTTTTCACTCAAAATTTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: ATP-PFK; Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKIGILTGGGDVPGLNPCMKAVVYHAIDDGHEIVGIRRGWGGLLNFNPDDPKSYDENIIPLDKQKVRTIDRTGGTFLHSS
RTNPSKVRVKDVPDFLRDPKTKNTDDASSDILDFTPHVLKNLEILGIDTLIPIGGDDTLSYGERLHREGVSVVAIPKTMD
NDVFGTDYCIGFSTAVTRSVQFIHQLRTSTGSHERIAVIELFGRNSGETSLISSYLSGVDRAIISEVPFDPEILASLLLK
DYNANPSSYAMVTISEGASMVGGKIIEYGEADAYGHRKLGGIGMITGEAVKKLTGKGIVYQQVGYLMRSGAPDALDLMIA
INFAGIAMGLLKDKVSGRMVALRDGTYTHIPISTIASGIKRVDVDELYDPKEYRPKVRNVLGKPMFLY

Sequences:

>Translated_388_residues
MKIGILTGGGDVPGLNPCMKAVVYHAIDDGHEIVGIRRGWGGLLNFNPDDPKSYDENIIPLDKQKVRTIDRTGGTFLHSS
RTNPSKVRVKDVPDFLRDPKTKNTDDASSDILDFTPHVLKNLEILGIDTLIPIGGDDTLSYGERLHREGVSVVAIPKTMD
NDVFGTDYCIGFSTAVTRSVQFIHQLRTSTGSHERIAVIELFGRNSGETSLISSYLSGVDRAIISEVPFDPEILASLLLK
DYNANPSSYAMVTISEGASMVGGKIIEYGEADAYGHRKLGGIGMITGEAVKKLTGKGIVYQQVGYLMRSGAPDALDLMIA
INFAGIAMGLLKDKVSGRMVALRDGTYTHIPISTIASGIKRVDVDELYDPKEYRPKVRNVLGKPMFLY
>Mature_388_residues
MKIGILTGGGDVPGLNPCMKAVVYHAIDDGHEIVGIRRGWGGLLNFNPDDPKSYDENIIPLDKQKVRTIDRTGGTFLHSS
RTNPSKVRVKDVPDFLRDPKTKNTDDASSDILDFTPHVLKNLEILGIDTLIPIGGDDTLSYGERLHREGVSVVAIPKTMD
NDVFGTDYCIGFSTAVTRSVQFIHQLRTSTGSHERIAVIELFGRNSGETSLISSYLSGVDRAIISEVPFDPEILASLLLK
DYNANPSSYAMVTISEGASMVGGKIIEYGEADAYGHRKLGGIGMITGEAVKKLTGKGIVYQQVGYLMRSGAPDALDLMIA
INFAGIAMGLLKDKVSGRMVALRDGTYTHIPISTIASGIKRVDVDELYDPKEYRPKVRNVLGKPMFLY

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=408, Percent_Identity=24.5098039215686, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI266453619, Length=403, Percent_Identity=24.317617866005, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI266453768, Length=403, Percent_Identity=24.317617866005, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI266453748, Length=403, Percent_Identity=24.317617866005, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4505749, Length=403, Percent_Identity=24.317617866005, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI48762920, Length=343, Percent_Identity=25.9475218658892, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1790350, Length=360, Percent_Identity=30.5555555555556, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25147584, Length=361, Percent_Identity=23.8227146814404, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17647809, Length=393, Percent_Identity=24.9363867684478, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652337, Length=393, Percent_Identity=24.9363867684478, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28573326, Length=391, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 42236; Mature: 42236

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGILTGGGDVPGLNPCMKAVVYHAIDDGHEIVGIRRGWGGLLNFNPDDPKSYDENIIP
CEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCC
LDKQKVRTIDRTGGTFLHSSRTNPSKVRVKDVPDFLRDPKTKNTDDASSDILDFTPHVLK
CCHHHHHHHHCCCCEEEECCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCHHHCCHHHHC
NLEILGIDTLIPIGGDDTLSYGERLHREGVSVVAIPKTMDNDVFGTDYCIGFSTAVTRSV
CCEEEEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEECHHHHHHHHH
QFIHQLRTSTGSHERIAVIELFGRNSGETSLISSYLSGVDRAIISEVPFDPEILASLLLK
HHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DYNANPSSYAMVTISEGASMVGGKIIEYGEADAYGHRKLGGIGMITGEAVKKLTGKGIVY
HCCCCCCCEEEEEECCCCCHHCCEEEECCCCCCCCCCCCCCEEEECHHHHHHHHCCCCHH
QQVGYLMRSGAPDALDLMIAINFAGIAMGLLKDKVSGRMVALRDGTYTHIPISTIASGIK
HHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCEEEEECCCEEEEEHHHHHHHHH
RVDVDELYDPKEYRPKVRNVLGKPMFLY
CCCHHHCCCCHHHCHHHHHHHCCCCCCC
>Mature Secondary Structure
MKIGILTGGGDVPGLNPCMKAVVYHAIDDGHEIVGIRRGWGGLLNFNPDDPKSYDENIIP
CEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCC
LDKQKVRTIDRTGGTFLHSSRTNPSKVRVKDVPDFLRDPKTKNTDDASSDILDFTPHVLK
CCHHHHHHHHCCCCEEEECCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCHHHCCHHHHC
NLEILGIDTLIPIGGDDTLSYGERLHREGVSVVAIPKTMDNDVFGTDYCIGFSTAVTRSV
CCEEEEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEECHHHHHHHHH
QFIHQLRTSTGSHERIAVIELFGRNSGETSLISSYLSGVDRAIISEVPFDPEILASLLLK
HHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DYNANPSSYAMVTISEGASMVGGKIIEYGEADAYGHRKLGGIGMITGEAVKKLTGKGIVY
HCCCCCCCEEEEEECCCCCHHCCEEEECCCCCCCCCCCCCCEEEECHHHHHHHHCCCCHH
QQVGYLMRSGAPDALDLMIAINFAGIAMGLLKDKVSGRMVALRDGTYTHIPISTIASGIK
HHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCEEEEECCCEEEEEHHHHHHHHH
RVDVDELYDPKEYRPKVRNVLGKPMFLY
CCCHHHCCCCHHHCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9055413; 12000953 [H]