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The map label for this gene is ispH

Identifier: 193213833

GI number: 193213833

Start: 133423

End: 134370

Strand: Direct

Name: ispH

Synonym: Ctha_0114

Alternate gene names: 193213833

Gene position: 133423-134370 (Clockwise)

Preceding gene: 193213832

Following gene: 193213834

Centisome position: 4.05

GC content: 44.83

Gene sequence:

>948_bases
ATGAAAGTCAATGTTGATATGAATTCTGGTTTTTGCTTTGGCGTCCAATTTGCCATTGATCGAGTTGAGGATGAAATGAA
AGCCTCTGGTCCGCTTTACTCGCTGGGTGATATTGTCCACAATGCGGTTGAAGTTGAGCGCCTTGAAAAATTGGGCTTAA
AGACCATTACCATAGAGGAATTTAAAACGCTTAAAAATACGCGGGTTTTTATTCGCGCACATGGCGAGCCACCTTCGACC
TATAAGCTGGCGCTTGAAAATAACATAGAGCTCATCGATGCCACCTGCCCGGTGGTAATGAAACTTCAGCAACGCATCAA
AGAATTTTACGATAGAGGCTATCAAGTTTTGATTTATGGCAGAGAAGGTCACCCTGAAGTGATCGGGCTATGCGGCCAGT
GCAACAACGAAGCGATCGTTTTGAAACACGCTGACCTTTCCGATCGAGGTGAAACTGAAAAAATCGATTTTTCTCGAAAA
ACCGTGCTCTTTTCTCAAACCACAAAAGACACAACCGGTTTTTATGAATTGAAAGCCAACTTGGAAAATGTATTTCGCGA
GCATCATGCTGCACAGAATTTTGATAGTGCTGCAACGGAATCAATTGTGCCGGATTTCCAAGCGAAAGATACCATTTGCC
GACAAGTTTCAAATCGCGACCAAAAGTTGGCCGCCTTTTCCAAAGAAAATGAATGCGTGATTTTTGTGGCTGGCAGGAAA
AGCTCGAACGGCAAAGTGCTTTTCGAGGTCTGCAAAGATGCCAATTCAAATACGCATTTTATAGAAAATCAATCCGAATT
GGAGCGAGCGTGGTTTTTGCGTCCCGACGATTCGCTTGTGGAAAGTGTTGGGGTTTGCGGCGCCACATCCACGCCGATGT
GGGTGATGCAGCAAGTGGCTGAACACATTCGCGCCACATTCGCTGCGGAAAGATTTGTTCAATCGTAA

Upstream 100 bases:

>100_bases
TGGCAACGGACGTGATTTCTCAGTTGAAATGAACACGCCCACCAACAGTTACTCTGTGTTTGAACGAAAATATGTTTAAC
CCAAACCGAGTTCTTACGCG

Downstream 100 bases:

>100_bases
TTCACTTTATTAACCTAAAACCCGTTCACTGGACTCTCGCGGTGAAACGGAAATATCTAATGAGAGGAAGGAAATAAACT
GCATGGCAGCATCACAAACA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKVNVDMNSGFCFGVQFAIDRVEDEMKASGPLYSLGDIVHNAVEVERLEKLGLKTITIEEFKTLKNTRVFIRAHGEPPST
YKLALENNIELIDATCPVVMKLQQRIKEFYDRGYQVLIYGREGHPEVIGLCGQCNNEAIVLKHADLSDRGETEKIDFSRK
TVLFSQTTKDTTGFYELKANLENVFREHHAAQNFDSAATESIVPDFQAKDTICRQVSNRDQKLAAFSKENECVIFVAGRK
SSNGKVLFEVCKDANSNTHFIENQSELERAWFLRPDDSLVESVGVCGATSTPMWVMQQVAEHIRATFAAERFVQS

Sequences:

>Translated_315_residues
MKVNVDMNSGFCFGVQFAIDRVEDEMKASGPLYSLGDIVHNAVEVERLEKLGLKTITIEEFKTLKNTRVFIRAHGEPPST
YKLALENNIELIDATCPVVMKLQQRIKEFYDRGYQVLIYGREGHPEVIGLCGQCNNEAIVLKHADLSDRGETEKIDFSRK
TVLFSQTTKDTTGFYELKANLENVFREHHAAQNFDSAATESIVPDFQAKDTICRQVSNRDQKLAAFSKENECVIFVAGRK
SSNGKVLFEVCKDANSNTHFIENQSELERAWFLRPDDSLVESVGVCGATSTPMWVMQQVAEHIRATFAAERFVQS
>Mature_315_residues
MKVNVDMNSGFCFGVQFAIDRVEDEMKASGPLYSLGDIVHNAVEVERLEKLGLKTITIEEFKTLKNTRVFIRAHGEPPST
YKLALENNIELIDATCPVVMKLQQRIKEFYDRGYQVLIYGREGHPEVIGLCGQCNNEAIVLKHADLSDRGETEKIDFSRK
TVLFSQTTKDTTGFYELKANLENVFREHHAAQNFDSAATESIVPDFQAKDTICRQVSNRDQKLAAFSKENECVIFVAGRK
SSNGKVLFEVCKDANSNTHFIENQSELERAWFLRPDDSLVESVGVCGATSTPMWVMQQVAEHIRATFAAERFVQS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=319, Percent_Identity=26.0188087774295, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_CHLT3 (B3QSU2)

Other databases:

- EMBL:   CP001100
- RefSeq:   YP_001995032.1
- GeneID:   6421286
- GenomeReviews:   CP001100_GR
- KEGG:   cts:Ctha_0114
- HOGENOM:   HBG335228
- OMA:   AAIHFKR
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 35546; Mature: 35546

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNVDMNSGFCFGVQFAIDRVEDEMKASGPLYSLGDIVHNAVEVERLEKLGLKTITIEE
CEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHH
FKTLKNTRVFIRAHGEPPSTYKLALENNIELIDATCPVVMKLQQRIKEFYDRGYQVLIYG
HHHHCCCEEEEEECCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEC
REGHPEVIGLCGQCNNEAIVLKHADLSDRGETEKIDFSRKTVLFSQTTKDTTGFYELKAN
CCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCEECCCCEEEEEECCCCCCCCHHEEHHH
LENVFREHHAAQNFDSAATESIVPDFQAKDTICRQVSNRDQKLAAFSKENECVIFVAGRK
HHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCC
SSNGKVLFEVCKDANSNTHFIENQSELERAWFLRPDDSLVESVGVCGATSTPMWVMQQVA
CCCCCEEEEEHHCCCCCCEEECCHHHHHHHEECCCCHHHHHHHCCCCCCCCHHHHHHHHH
EHIRATFAAERFVQS
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVNVDMNSGFCFGVQFAIDRVEDEMKASGPLYSLGDIVHNAVEVERLEKLGLKTITIEE
CEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHH
FKTLKNTRVFIRAHGEPPSTYKLALENNIELIDATCPVVMKLQQRIKEFYDRGYQVLIYG
HHHHCCCEEEEEECCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEC
REGHPEVIGLCGQCNNEAIVLKHADLSDRGETEKIDFSRKTVLFSQTTKDTTGFYELKAN
CCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCEECCCCEEEEEECCCCCCCCHHEEHHH
LENVFREHHAAQNFDSAATESIVPDFQAKDTICRQVSNRDQKLAAFSKENECVIFVAGRK
HHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCC
SSNGKVLFEVCKDANSNTHFIENQSELERAWFLRPDDSLVESVGVCGATSTPMWVMQQVA
CCCCCEEEEEHHCCCCCCEEECCHHHHHHHEECCCCHHHHHHHCCCCCCCCHHHHHHHHH
EHIRATFAAERFVQS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA