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The map label for this gene is dosC [H]

Identifier: 193213747

GI number: 193213747

Start: 37080

End: 38798

Strand: Direct

Name: dosC [H]

Synonym: Ctha_0027

Alternate gene names: 193213747

Gene position: 37080-38798 (Clockwise)

Preceding gene: 193213746

Following gene: 193213748

Centisome position: 1.13

GC content: 43.86

Gene sequence:

>1719_bases
ATGAAAATCTCAACTACACAGTCAAAGATCGATAATATTAAGAAACGGCTGGATTCCAACATCGATGAAAAAGAGCGCGT
AGACTTGCTGGTTTCTCTTGCCGAAACGATTCACATCACCAACACGCAATATGCACTTGACTTAGGAAAACGCGCCTGCG
ATCAGGCCAAAGAATTGTGCTACAAAAAAGGTCAAGCCAAAGGCTTGTGCATTATTGGCAAATGCCTCTGGCGGCTTTCG
AACAATGAAGAAGCCCTCACCCACTTACTCGAATCCCTTTGGACATTTGAAGAAATCGGCGAACGCACCGGCGAGGCCAA
AGCCCTTATGGCAATGGGCGTTGTTCTAAAAAGCCTGAGCGATTTCGAACAGGCCATCGGCTATTTTTTCCAAAGTCTTC
GAATTTTGGAGGAAGTCAACGATAAAGTTAGCCAAGCCGAAGTGATGAACAACATCGGCAACGTGTTTAAAAATCTGAAC
AATTACTACGAAGCGCTGCCCTATTACCAACAAAGTTTAGCTATTCTCGAGTCCGCAAATGAAAAACATGGGATGGCACT
TGCTCTTAACAACATCGGCTACGTGCACTACGCGTTAGGCAAATATGATGAAGCGTTGCCCTATTTTCAGCAAAGTATTA
AGTATGCAAAAGAAATCGGCGATGCCATTGCAGAGTCTGAAGCGCTGAACAACATGGGCATGGTTTATGAAAAAATTGTT
GACGACCAAACGGCGCTGGATATTTATTTCAAAAGCCTTTTGAAATCGCAGGAAATCGGCGATCGCTATGGAGAAGCGCG
CGTGCTGCTTTCCATTGGGATGCAGTACGCCAAAAATAAAGACATTGAAAGCGCAGAGCAGTATTTGCAAGAATCGACCT
TGATTGCGGAGGCCGTTAAAGCAAGAAGCATTATGTGCCAAGCGTATCGCGCCCTTGCCAATGTTTATGAGCAGAAAAAA
GATTTTGAGCGAGCGTTAGGCTACTATAAGGCGTTCGATAGCATCAAAGAAGAGCTTTTAAGTAAAGACATCACGGGAAA
AATCAACGCACTTAAGCAGAATTTTGAGCTTCACACCGTTCAAAAAGAACTCGAGCTATTCCGCACCAAAAATGAAGAGT
TGATAGGCGCTTTTCGCGACTTGCAATCGCTCAATATTTCACTTCAAGCCGAGCTTGATTTAAAGACACGCGTTGTGAAT
GAACTTCGTTCTAAAAATGACGCAAAAGATGCTGAACCACGAAAAGACTTTTTAACAAACCTTTACACCCTGCGCGCCGT
TTCTCCAAAGTTGAAACATTTGTTTGATTCTGCCAAGCGGCAAGGAAAAGAGCTAAGTATAGCAGTTATTGAAATTGACT
TGTTTAAACAAATCAACAGCCGCTATCCAAGCCAAATCATGAGTGAAGTTTTAAAAAAAGTGGCCCAAATTCTAAAGGAG
CATATCCGAACAGAAGACATTTTGGTACGCTATCTTGGCAGTTCTTTTGTGCTACTGATGCCCGATTTGCCAGCTGTTCG
TGCTTTTATTATTTGTGAAAAAATGCGCCAGGCCGTTGAGTCGTTTCGCTGGGGCATGCTGGCTTCTGGACTAAAAATAA
CCGTGAGTTTCGGCTTAGCCGACGATCTAAAAGTGCCGAGCTATGAAAAGCTCATTCGCTTAGCCGAAATGAAGCTTCAA
CAAACCGAGCGCTCAGGCGGCAACCAAGTCAGGAGCTAA

Upstream 100 bases:

>100_bases
CAACCGACAAAATGAGGGAGAATGGCGTCAATCGATGATGACATTTAGATATAATGATTTATGAGGAAATAGCATGGTAG
AATTTTAGTAGAGAACAATC

Downstream 100 bases:

>100_bases
GCTTTTTATTGACAAATTGCCAAATTGATTGGGAAAAAAAGTTTTTTTGAATAATTTAATTTTTGACAGTTGTATGTATA
TGGCTATGGTTGTTTTAGAA

Product: diguanylate cyclase/serine/threonine protein kinase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MKISTTQSKIDNIKKRLDSNIDEKERVDLLVSLAETIHITNTQYALDLGKRACDQAKELCYKKGQAKGLCIIGKCLWRLS
NNEEALTHLLESLWTFEEIGERTGEAKALMAMGVVLKSLSDFEQAIGYFFQSLRILEEVNDKVSQAEVMNNIGNVFKNLN
NYYEALPYYQQSLAILESANEKHGMALALNNIGYVHYALGKYDEALPYFQQSIKYAKEIGDAIAESEALNNMGMVYEKIV
DDQTALDIYFKSLLKSQEIGDRYGEARVLLSIGMQYAKNKDIESAEQYLQESTLIAEAVKARSIMCQAYRALANVYEQKK
DFERALGYYKAFDSIKEELLSKDITGKINALKQNFELHTVQKELELFRTKNEELIGAFRDLQSLNISLQAELDLKTRVVN
ELRSKNDAKDAEPRKDFLTNLYTLRAVSPKLKHLFDSAKRQGKELSIAVIEIDLFKQINSRYPSQIMSEVLKKVAQILKE
HIRTEDILVRYLGSSFVLLMPDLPAVRAFIICEKMRQAVESFRWGMLASGLKITVSFGLADDLKVPSYEKLIRLAEMKLQ
QTERSGGNQVRS

Sequences:

>Translated_572_residues
MKISTTQSKIDNIKKRLDSNIDEKERVDLLVSLAETIHITNTQYALDLGKRACDQAKELCYKKGQAKGLCIIGKCLWRLS
NNEEALTHLLESLWTFEEIGERTGEAKALMAMGVVLKSLSDFEQAIGYFFQSLRILEEVNDKVSQAEVMNNIGNVFKNLN
NYYEALPYYQQSLAILESANEKHGMALALNNIGYVHYALGKYDEALPYFQQSIKYAKEIGDAIAESEALNNMGMVYEKIV
DDQTALDIYFKSLLKSQEIGDRYGEARVLLSIGMQYAKNKDIESAEQYLQESTLIAEAVKARSIMCQAYRALANVYEQKK
DFERALGYYKAFDSIKEELLSKDITGKINALKQNFELHTVQKELELFRTKNEELIGAFRDLQSLNISLQAELDLKTRVVN
ELRSKNDAKDAEPRKDFLTNLYTLRAVSPKLKHLFDSAKRQGKELSIAVIEIDLFKQINSRYPSQIMSEVLKKVAQILKE
HIRTEDILVRYLGSSFVLLMPDLPAVRAFIICEKMRQAVESFRWGMLASGLKITVSFGLADDLKVPSYEKLIRLAEMKLQ
QTERSGGNQVRS
>Mature_572_residues
MKISTTQSKIDNIKKRLDSNIDEKERVDLLVSLAETIHITNTQYALDLGKRACDQAKELCYKKGQAKGLCIIGKCLWRLS
NNEEALTHLLESLWTFEEIGERTGEAKALMAMGVVLKSLSDFEQAIGYFFQSLRILEEVNDKVSQAEVMNNIGNVFKNLN
NYYEALPYYQQSLAILESANEKHGMALALNNIGYVHYALGKYDEALPYFQQSIKYAKEIGDAIAESEALNNMGMVYEKIV
DDQTALDIYFKSLLKSQEIGDRYGEARVLLSIGMQYAKNKDIESAEQYLQESTLIAEAVKARSIMCQAYRALANVYEQKK
DFERALGYYKAFDSIKEELLSKDITGKINALKQNFELHTVQKELELFRTKNEELIGAFRDLQSLNISLQAELDLKTRVVN
ELRSKNDAKDAEPRKDFLTNLYTLRAVSPKLKHLFDSAKRQGKELSIAVIEIDLFKQINSRYPSQIMSEVLKKVAQILKE
HIRTEDILVRYLGSSFVLLMPDLPAVRAFIICEKMRQAVESFRWGMLASGLKITVSFGLADDLKVPSYEKLIRLAEMKLQ
QTERSGGNQVRS

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Homo sapiens, GI224028289, Length=316, Percent_Identity=26.5822784810127, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI164519122, Length=300, Percent_Identity=28.3333333333333, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI224548927, Length=303, Percent_Identity=28.0528052805281, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI224548925, Length=303, Percent_Identity=28.0528052805281, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=30.5732484076433, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=23.4177215189873, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25147165, Length=276, Percent_Identity=21.7391304347826, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24650658, Length=296, Percent_Identity=24.3243243243243, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24660950, Length=289, Percent_Identity=22.8373702422145, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 65151; Mature: 65151

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50005 TPR ; PS50293 TPR_REGION ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISTTQSKIDNIKKRLDSNIDEKERVDLLVSLAETIHITNTQYALDLGKRACDQAKELC
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YKKGQAKGLCIIGKCLWRLSNNEEALTHLLESLWTFEEIGERTGEAKALMAMGVVLKSLS
HHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
DFEQAIGYFFQSLRILEEVNDKVSQAEVMNNIGNVFKNLNNYYEALPYYQQSLAILESAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EKHGMALALNNIGYVHYALGKYDEALPYFQQSIKYAKEIGDAIAESEALNNMGMVYEKIV
CCCCEEEEECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDQTALDIYFKSLLKSQEIGDRYGEARVLLSIGMQYAKNKDIESAEQYLQESTLIAEAVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ARSIMCQAYRALANVYEQKKDFERALGYYKAFDSIKEELLSKDITGKINALKQNFELHTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHH
QKELELFRTKNEELIGAFRDLQSLNISLQAELDLKTRVVNELRSKNDAKDAEPRKDFLTN
HHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHH
LYTLRAVSPKLKHLFDSAKRQGKELSIAVIEIDLFKQINSRYPSQIMSEVLKKVAQILKE
HHHHHHCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
HIRTEDILVRYLGSSFVLLMPDLPAVRAFIICEKMRQAVESFRWGMLASGLKITVSFGLA
HHCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
DDLKVPSYEKLIRLAEMKLQQTERSGGNQVRS
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKISTTQSKIDNIKKRLDSNIDEKERVDLLVSLAETIHITNTQYALDLGKRACDQAKELC
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YKKGQAKGLCIIGKCLWRLSNNEEALTHLLESLWTFEEIGERTGEAKALMAMGVVLKSLS
HHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
DFEQAIGYFFQSLRILEEVNDKVSQAEVMNNIGNVFKNLNNYYEALPYYQQSLAILESAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EKHGMALALNNIGYVHYALGKYDEALPYFQQSIKYAKEIGDAIAESEALNNMGMVYEKIV
CCCCEEEEECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDQTALDIYFKSLLKSQEIGDRYGEARVLLSIGMQYAKNKDIESAEQYLQESTLIAEAVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ARSIMCQAYRALANVYEQKKDFERALGYYKAFDSIKEELLSKDITGKINALKQNFELHTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHH
QKELELFRTKNEELIGAFRDLQSLNISLQAELDLKTRVVNELRSKNDAKDAEPRKDFLTN
HHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHH
LYTLRAVSPKLKHLFDSAKRQGKELSIAVIEIDLFKQINSRYPSQIMSEVLKKVAQILKE
HHHHHHCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
HIRTEDILVRYLGSSFVLLMPDLPAVRAFIICEKMRQAVESFRWGMLASGLKITVSFGLA
HHCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
DDLKVPSYEKLIRLAEMKLQQTERSGGNQVRS
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]