Click here to switch to the map view.

The map label for this gene is hisS

Identifier: 193213734

GI number: 193213734

Start: 17545

End: 18828

Strand: Direct

Name: hisS

Synonym: Ctha_0014

Alternate gene names: 193213734

Gene position: 17545-18828 (Clockwise)

Preceding gene: 193213733

Following gene: 193213736

Centisome position: 0.53

GC content: 48.83

Gene sequence:

>1284_bases
ATGAAGTTTCAGACCATCAAAGGGACCAAAGACATCCTACCTTCAGAAATTCACAAATGGCATTACGTAGAAAACACTGT
TCGAGGCGTGTTTCAACGCTTCGGATACAACGAAATTCGTACGCCTGTTTTTGAGCAAACCGCGCTTTTTCAACGCGGAA
TTGGGGAGACAACCGACATCGTAGGCAAAGAAATGTACTCATTTCAGCCCGATCCTGAATCAGAATCCCTAACGCTTCGT
CCCGAAATGACAGCGCCGGTTATGCGCGCATATCTACAACACAGCCTTTCTGGAACATCTCCGGCAACCAAAGTTTTCTA
CATCAGCGAGATTTTTCGAAAAGAGCGCCCGCAGGCTGGCCGCCAACGCCAGTTCTGGCAATTTGGCTGCGAATGTATCG
GCAGCGACCAGCCCGAAGCCGATGCAGAAGTCATCTTGCTCATGACTGAAATTTACCGGCAACTTGGCATTAAAAATTTC
ACCTTGCGGCTGAATAGTTTAGGCGAAACGGAATCCAGACTTGCCCACCGCGAAGCGCTCCAAACGTATTTGAAACCTCA
TTTTGACCTTTTAGATGAAATTTCAAAAACGCGCTTCGAGAAAAATCCGCTTAGAATTCTCGATTCAAAAAATCCCGCTT
TGGCTGAAATTATTGCTGGCGCACCGCACATTACCGAGTTTCTTGACGATGCCTCCAAAGCGCATTTTCAAACGGTGCAA
ACCTATCTAAAAAATGCCGGCCTCGACTTTACAGTTGACCCAACGCTCGTTCGCGGACTGGATTATTACTCCAGAACGGC
GTTTGAACTCGTCAGCACCGACTTAGGAGCGCAGGACGCGCTGGCTGGCGGTGGCCGCTATGATTCGCTGGCGACTGTGC
TTGGCGCTAAAAATAGCTCAGCTGCAGTTGGTTTTGCAGCCGGCATAGAGCGACTCTTGATTATCATGGAAAAGCTGGAT
TTATTTCAAGCCGTGCTGCCACCTGCGCCATTGCTTTTCATAGCAACGCAAAGTCCGGTTGCAAAGGAATGGGCGTTCCA
AACCGTCAATCGCCTTCGCACGGAGGGAATTCATGTCGCGCTTGATTTGCTTGGCCGAAGCTTGAAAGCACAAATGAGAG
AAGCCAACCGAACCCATGCGAAATACGTGCTGATCGTTGGCGAAGAAGAACTTTCAACCGGGCGCTTCCAATTGAAGCAT
TTGCAAACTTCGGAGCAGGTCGAACTTTCGGAAGCAGACATTTTCACGAAAATGCAAACCGAAACCACACAGGATCTCGG
TTAA

Upstream 100 bases:

>100_bases
AAATAGACTTTTGGAGACCAAAGACCCAGCTTCTGTGCTTTCTGTCTTTATTGAAGAAGAAAAAGCCTTCCCTGAAATTT
AGTTGTTAAAAAAGTCCAGT

Downstream 100 bases:

>100_bases
TTTTTTCTACAAAAATCCCCCATTTGACTTTCTAAAATCGTGCTGATCGGAAAAGACGAAGCGCTTGCTACTTAAGCACT
TTGATCACTTTCACCTCAAT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKFQTIKGTKDILPSEIHKWHYVENTVRGVFQRFGYNEIRTPVFEQTALFQRGIGETTDIVGKEMYSFQPDPESESLTLR
PEMTAPVMRAYLQHSLSGTSPATKVFYISEIFRKERPQAGRQRQFWQFGCECIGSDQPEADAEVILLMTEIYRQLGIKNF
TLRLNSLGETESRLAHREALQTYLKPHFDLLDEISKTRFEKNPLRILDSKNPALAEIIAGAPHITEFLDDASKAHFQTVQ
TYLKNAGLDFTVDPTLVRGLDYYSRTAFELVSTDLGAQDALAGGGRYDSLATVLGAKNSSAAVGFAAGIERLLIIMEKLD
LFQAVLPPAPLLFIATQSPVAKEWAFQTVNRLRTEGIHVALDLLGRSLKAQMREANRTHAKYVLIVGEEELSTGRFQLKH
LQTSEQVELSEADIFTKMQTETTQDLG

Sequences:

>Translated_427_residues
MKFQTIKGTKDILPSEIHKWHYVENTVRGVFQRFGYNEIRTPVFEQTALFQRGIGETTDIVGKEMYSFQPDPESESLTLR
PEMTAPVMRAYLQHSLSGTSPATKVFYISEIFRKERPQAGRQRQFWQFGCECIGSDQPEADAEVILLMTEIYRQLGIKNF
TLRLNSLGETESRLAHREALQTYLKPHFDLLDEISKTRFEKNPLRILDSKNPALAEIIAGAPHITEFLDDASKAHFQTVQ
TYLKNAGLDFTVDPTLVRGLDYYSRTAFELVSTDLGAQDALAGGGRYDSLATVLGAKNSSAAVGFAAGIERLLIIMEKLD
LFQAVLPPAPLLFIATQSPVAKEWAFQTVNRLRTEGIHVALDLLGRSLKAQMREANRTHAKYVLIVGEEELSTGRFQLKH
LQTSEQVELSEADIFTKMQTETTQDLG
>Mature_427_residues
MKFQTIKGTKDILPSEIHKWHYVENTVRGVFQRFGYNEIRTPVFEQTALFQRGIGETTDIVGKEMYSFQPDPESESLTLR
PEMTAPVMRAYLQHSLSGTSPATKVFYISEIFRKERPQAGRQRQFWQFGCECIGSDQPEADAEVILLMTEIYRQLGIKNF
TLRLNSLGETESRLAHREALQTYLKPHFDLLDEISKTRFEKNPLRILDSKNPALAEIIAGAPHITEFLDDASKAHFQTVQ
TYLKNAGLDFTVDPTLVRGLDYYSRTAFELVSTDLGAQDALAGGGRYDSLATVLGAKNSSAAVGFAAGIERLLIIMEKLD
LFQAVLPPAPLLFIATQSPVAKEWAFQTVNRLRTEGIHVALDLLGRSLKAQMREANRTHAKYVLIVGEEELSTGRFQLKH
LQTSEQVELSEADIFTKMQTETTQDLG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=461, Percent_Identity=25.3796095444685, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI15029520, Length=460, Percent_Identity=22.8260869565217, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1788861, Length=427, Percent_Identity=40.9836065573771, Blast_Score=327, Evalue=6e-91,
Organism=Caenorhabditis elegans, GI71993693, Length=452, Percent_Identity=24.7787610619469, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71993686, Length=452, Percent_Identity=24.7787610619469, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6325290, Length=503, Percent_Identity=23.6580516898608, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24643061, Length=476, Percent_Identity=23.9495798319328, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_CHLT3 (B3QS95)

Other databases:

- EMBL:   CP001100
- RefSeq:   YP_001994933.1
- GeneID:   6421186
- GenomeReviews:   CP001100_GR
- KEGG:   cts:Ctha_0014
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48069; Mature: 48069

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFQTIKGTKDILPSEIHKWHYVENTVRGVFQRFGYNEIRTPVFEQTALFQRGIGETTDI
CCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCHHHH
VGKEMYSFQPDPESESLTLRPEMTAPVMRAYLQHSLSGTSPATKVFYISEIFRKERPQAG
HHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
RQRQFWQFGCECIGSDQPEADAEVILLMTEIYRQLGIKNFTLRLNSLGETESRLAHREAL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
QTYLKPHFDLLDEISKTRFEKNPLRILDSKNPALAEIIAGAPHITEFLDDASKAHFQTVQ
HHHHCHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
TYLKNAGLDFTVDPTLVRGLDYYSRTAFELVSTDLGAQDALAGGGRYDSLATVLGAKNSS
HHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHCCCCCC
AAVGFAAGIERLLIIMEKLDLFQAVLPPAPLLFIATQSPVAKEWAFQTVNRLRTEGIHVA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHH
LDLLGRSLKAQMREANRTHAKYVLIVGEEELSTGRFQLKHLQTSEQVELSEADIFTKMQT
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
ETTQDLG
HHHHCCC
>Mature Secondary Structure
MKFQTIKGTKDILPSEIHKWHYVENTVRGVFQRFGYNEIRTPVFEQTALFQRGIGETTDI
CCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCHHHH
VGKEMYSFQPDPESESLTLRPEMTAPVMRAYLQHSLSGTSPATKVFYISEIFRKERPQAG
HHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
RQRQFWQFGCECIGSDQPEADAEVILLMTEIYRQLGIKNFTLRLNSLGETESRLAHREAL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
QTYLKPHFDLLDEISKTRFEKNPLRILDSKNPALAEIIAGAPHITEFLDDASKAHFQTVQ
HHHHCHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
TYLKNAGLDFTVDPTLVRGLDYYSRTAFELVSTDLGAQDALAGGGRYDSLATVLGAKNSS
HHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHCCCCCC
AAVGFAAGIERLLIIMEKLDLFQAVLPPAPLLFIATQSPVAKEWAFQTVNRLRTEGIHVA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHH
LDLLGRSLKAQMREANRTHAKYVLIVGEEELSTGRFQLKHLQTSEQVELSEADIFTKMQT
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
ETTQDLG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA