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The map label for this gene is dnaG [H]

Identifier: 193213161

GI number: 193213161

Start: 1657841

End: 1659739

Strand: Direct

Name: dnaG [H]

Synonym: Cpar_1516

Alternate gene names: 193213161

Gene position: 1657841-1659739 (Clockwise)

Preceding gene: 193213156

Following gene: 193213165

Centisome position: 72.42

GC content: 57.98

Gene sequence:

>1899_bases
ATGATCCCCCAACGCATGATTGATGAAGTCCGACAGAGTTCGGACATTGTTGACATAGTGTCGGATTATGTCAGGCTCAA
TCCGTCAGGCCGCCACTTCAAGGCCCTCTCCCCCTTCACGCAGGAGAAAACCCCCTCCTTCATCGTTTCGCCCGACAAGC
AGATCTACAAATGCTTTTCGAGCGGCAAGGGAGGCAACGTCTTCACCTTCATCATGGAGATGGAGAAGGTGACTTTCCCC
GAAGCGGTCGAGATGCTGGCCAAGCGCGCCGGAGTGGACACCAGCAAATACCAGCTGCAGAAAGCGCAACAGGAAGACAA
ACAGGAAAGCAACCAGCATGAAACGCTCCGCTGGGCAGCCCGGTTGTTTCACCAAACTCTGGAGAGCGATGCAGGCAGGG
CGGCGTATGCCTATCTCGTCGGCAAGCGTGGACTGGAAGCGAGCACGATCCGCAAATTCGGCCTCGGCTTTGCACCCGAA
GCGTGGGAGTTCCTGCTCGACGCTGCCCGGCGTGACAACATTCCGGTCGAGCAGCTCACCGCCCTCGGCCTCGTGACGCG
CCACCGGGAGCGCAACAGTCTATACGACACCTTCCGCAACCGGATCATCTTTCCGATTCTGACTGTCGGCGGACAGGTGG
CCGGCTTCGGCGGCCGGACGCTCTCGAACGATCCGCAGACACCGAAGTACATCAACTCGCCGGAGAGCGCCATTTTCGAA
AAGTCGAAACTGCTCTACGGAATGCACGCCGCCAAAAACGACATCCGCAAGCAGGAGACGGCAATTCTCGTGGAAGGCTA
CATGGACGTGATCGCCATGCACCAGGCCGGAGTCACCAACACGGTGGCCTCCTCGGGCACGGCACTCACCCGCTACCAGG
CAAAAATTCTCAAGCGCTACACCTCGCGCGTTCTTTTTCTGTACGACGGCGACAACGCGGGCAAAAAGTCGATGCTTGCC
GGCATCGACATCCTGCTCTCCGAGGGGCTTACCCCGTGGGTAGTCCTCCTGCCCGGTGGCGACGACCCCGACAGCTTCAT
CAGAAACTATGGCAAGGAGGCGTTTCTCGAAGAGCTCGAAAGTGAGAAAAGCTCATTCCAGGATTTCCAGCTCCGCTGTT
ATCGCGACGCCGGGCTCATGGAAAACCCTGATACCGCCTCCAAAGCGATCTCGGCCATGACCAAAAGCATCGCCTTGATC
GCCGACCCCGTCCAAAAGGAGTTATACCTCGAAGAGCTGTCGAAAAAGCTTGACCTCAGCCGCCAGACGCTCCGCAAGGT
GATGGCCTCCAGCAACGATGATCGTGCGCCTCGCTACGAATCACGGGGGCCAAGCCAGCAGAAAAGCTCGCCCGAAGTGA
AGGCCGATAATCGCCCGATCTCTGTGGCCGAGCGCACCTTTCTCGAAGCGCTGCTCGAAAGCACCTTCTACGGCAACGAA
GTGCTCGATTTCGCCGCTTCGCACGAATCGATGTTCACGCTTGAACACCCTGCTGCACGAGCAATCTTTACGCATCTGGT
CGAACGCTTCCGCGAGATGGACGACAAAGAAGGCCACATCGACATCACCTCGGAAATCAGCTCCCTCGGCCTGGAAGAAG
CACGCAACATCGCGCTCGACATCCTCTTCCGACTTCCGGTCAGCGAAACCAACCTCCCCTCCCCGCAGGAAGCCGCCCAG
CACGCCCGCCGCTGCCTTTCTCACTTCCTCGTCGCAGTCAAAACCATCGTACTCGAACCGCTGCAACGCCAGAAGCAGGA
CATTCTCAACCGCCTCAACAGCACATCTGAAAAGCCGGAAGAGAAAAAGCTTTTCGCGCAACTCCACGACATCAACAACC
AGCTCCGCGAAATGGAGCAGGAGGTTGACGAGTCGATCAGAGGGATTCTGGGAGAATAA

Upstream 100 bases:

>100_bases
GAAAACGTCCTGTTTAAAACCGGGAAGAACGGATTGTGATTTATGTATTTTAGGACTATCATAACACCGCAAACCAAGTC
ACCCGGAAGAAAAGTCGCCC

Downstream 100 bases:

>100_bases
AACTTTATCTATTCGATTCGAGAGCTTCTAATAAATATTTCTTATCCAACATCTATTAACGAAAGCAACAGAGCAATATG
AGGCATTCCATAAAGCCAGC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MIPQRMIDEVRQSSDIVDIVSDYVRLNPSGRHFKALSPFTQEKTPSFIVSPDKQIYKCFSSGKGGNVFTFIMEMEKVTFP
EAVEMLAKRAGVDTSKYQLQKAQQEDKQESNQHETLRWAARLFHQTLESDAGRAAYAYLVGKRGLEASTIRKFGLGFAPE
AWEFLLDAARRDNIPVEQLTALGLVTRHRERNSLYDTFRNRIIFPILTVGGQVAGFGGRTLSNDPQTPKYINSPESAIFE
KSKLLYGMHAAKNDIRKQETAILVEGYMDVIAMHQAGVTNTVASSGTALTRYQAKILKRYTSRVLFLYDGDNAGKKSMLA
GIDILLSEGLTPWVVLLPGGDDPDSFIRNYGKEAFLEELESEKSSFQDFQLRCYRDAGLMENPDTASKAISAMTKSIALI
ADPVQKELYLEELSKKLDLSRQTLRKVMASSNDDRAPRYESRGPSQQKSSPEVKADNRPISVAERTFLEALLESTFYGNE
VLDFAASHESMFTLEHPAARAIFTHLVERFREMDDKEGHIDITSEISSLGLEEARNIALDILFRLPVSETNLPSPQEAAQ
HARRCLSHFLVAVKTIVLEPLQRQKQDILNRLNSTSEKPEEKKLFAQLHDINNQLREMEQEVDESIRGILGE

Sequences:

>Translated_632_residues
MIPQRMIDEVRQSSDIVDIVSDYVRLNPSGRHFKALSPFTQEKTPSFIVSPDKQIYKCFSSGKGGNVFTFIMEMEKVTFP
EAVEMLAKRAGVDTSKYQLQKAQQEDKQESNQHETLRWAARLFHQTLESDAGRAAYAYLVGKRGLEASTIRKFGLGFAPE
AWEFLLDAARRDNIPVEQLTALGLVTRHRERNSLYDTFRNRIIFPILTVGGQVAGFGGRTLSNDPQTPKYINSPESAIFE
KSKLLYGMHAAKNDIRKQETAILVEGYMDVIAMHQAGVTNTVASSGTALTRYQAKILKRYTSRVLFLYDGDNAGKKSMLA
GIDILLSEGLTPWVVLLPGGDDPDSFIRNYGKEAFLEELESEKSSFQDFQLRCYRDAGLMENPDTASKAISAMTKSIALI
ADPVQKELYLEELSKKLDLSRQTLRKVMASSNDDRAPRYESRGPSQQKSSPEVKADNRPISVAERTFLEALLESTFYGNE
VLDFAASHESMFTLEHPAARAIFTHLVERFREMDDKEGHIDITSEISSLGLEEARNIALDILFRLPVSETNLPSPQEAAQ
HARRCLSHFLVAVKTIVLEPLQRQKQDILNRLNSTSEKPEEKKLFAQLHDINNQLREMEQEVDESIRGILGE
>Mature_632_residues
MIPQRMIDEVRQSSDIVDIVSDYVRLNPSGRHFKALSPFTQEKTPSFIVSPDKQIYKCFSSGKGGNVFTFIMEMEKVTFP
EAVEMLAKRAGVDTSKYQLQKAQQEDKQESNQHETLRWAARLFHQTLESDAGRAAYAYLVGKRGLEASTIRKFGLGFAPE
AWEFLLDAARRDNIPVEQLTALGLVTRHRERNSLYDTFRNRIIFPILTVGGQVAGFGGRTLSNDPQTPKYINSPESAIFE
KSKLLYGMHAAKNDIRKQETAILVEGYMDVIAMHQAGVTNTVASSGTALTRYQAKILKRYTSRVLFLYDGDNAGKKSMLA
GIDILLSEGLTPWVVLLPGGDDPDSFIRNYGKEAFLEELESEKSSFQDFQLRCYRDAGLMENPDTASKAISAMTKSIALI
ADPVQKELYLEELSKKLDLSRQTLRKVMASSNDDRAPRYESRGPSQQKSSPEVKADNRPISVAERTFLEALLESTFYGNE
VLDFAASHESMFTLEHPAARAIFTHLVERFREMDDKEGHIDITSEISSLGLEEARNIALDILFRLPVSETNLPSPQEAAQ
HARRCLSHFLVAVKTIVLEPLQRQKQDILNRLNSTSEKPEEKKLFAQLHDINNQLREMEQEVDESIRGILGE

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=427, Percent_Identity=35.5971896955504, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 71230; Mature: 71230

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPQRMIDEVRQSSDIVDIVSDYVRLNPSGRHFKALSPFTQEKTPSFIVSPDKQIYKCFS
CCCHHHHHHHHHCCHHHHHHHHHHEECCCCCCEECCCCCCCCCCCCEEECCHHHHHHHHH
SGKGGNVFTFIMEMEKVTFPEAVEMLAKRAGVDTSKYQLQKAQQEDKQESNQHETLRWAA
CCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLFHQTLESDAGRAAYAYLVGKRGLEASTIRKFGLGFAPEAWEFLLDAARRDNIPVEQLT
HHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHH
ALGLVTRHRERNSLYDTFRNRIIFPILTVGGQVAGFGGRTLSNDPQTPKYINSPESAIFE
HHHHHHHHHHHHHHHHHHHHCEEHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHH
KSKLLYGMHAAKNDIRKQETAILVEGYMDVIAMHQAGVTNTVASSGTALTRYQAKILKRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHH
TSRVLFLYDGDNAGKKSMLAGIDILLSEGLTPWVVLLPGGDDPDSFIRNYGKEAFLEELE
HCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCHHHHHHHHH
SEKSSFQDFQLRCYRDAGLMENPDTASKAISAMTKSIALIADPVQKELYLEELSKKLDLS
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RQTLRKVMASSNDDRAPRYESRGPSQQKSSPEVKADNRPISVAERTFLEALLESTFYGNE
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
VLDFAASHESMFTLEHPAARAIFTHLVERFREMDDKEGHIDITSEISSLGLEEARNIALD
HHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCHHHHHHHHHH
ILFRLPVSETNLPSPQEAAQHARRCLSHFLVAVKTIVLEPLQRQKQDILNRLNSTSEKPE
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
EKKLFAQLHDINNQLREMEQEVDESIRGILGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPQRMIDEVRQSSDIVDIVSDYVRLNPSGRHFKALSPFTQEKTPSFIVSPDKQIYKCFS
CCCHHHHHHHHHCCHHHHHHHHHHEECCCCCCEECCCCCCCCCCCCEEECCHHHHHHHHH
SGKGGNVFTFIMEMEKVTFPEAVEMLAKRAGVDTSKYQLQKAQQEDKQESNQHETLRWAA
CCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLFHQTLESDAGRAAYAYLVGKRGLEASTIRKFGLGFAPEAWEFLLDAARRDNIPVEQLT
HHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHH
ALGLVTRHRERNSLYDTFRNRIIFPILTVGGQVAGFGGRTLSNDPQTPKYINSPESAIFE
HHHHHHHHHHHHHHHHHHHHCEEHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHH
KSKLLYGMHAAKNDIRKQETAILVEGYMDVIAMHQAGVTNTVASSGTALTRYQAKILKRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHH
TSRVLFLYDGDNAGKKSMLAGIDILLSEGLTPWVVLLPGGDDPDSFIRNYGKEAFLEELE
HCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCHHHHHHHHH
SEKSSFQDFQLRCYRDAGLMENPDTASKAISAMTKSIALIADPVQKELYLEELSKKLDLS
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RQTLRKVMASSNDDRAPRYESRGPSQQKSSPEVKADNRPISVAERTFLEALLESTFYGNE
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
VLDFAASHESMFTLEHPAARAIFTHLVERFREMDDKEGHIDITSEISSLGLEEARNIALD
HHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCHHHHHHHHHH
ILFRLPVSETNLPSPQEAAQHARRCLSHFLVAVKTIVLEPLQRQKQDILNRLNSTSEKPE
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
EKKLFAQLHDINNQLREMEQEVDESIRGILGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]