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The map label for this gene is aprF [H]

Identifier: 193212503

GI number: 193212503

Start: 914984

End: 916309

Strand: Direct

Name: aprF [H]

Synonym: Cpar_0840

Alternate gene names: 193212503

Gene position: 914984-916309 (Clockwise)

Preceding gene: 193212502

Following gene: 193212504

Centisome position: 39.97

GC content: 55.88

Gene sequence:

>1326_bases
ATGGCGTTTTTGCTTGTTCTCGTTCAGTTCTTTTTGCCAATGGCAGCACAGGCCAAGTCCCTCTCGTTGAAAGAGGCCTA
CGAACATGCGTTGCAGTATGACGCGCAGTACCGTTCCGCCGAGGCCGACACCCGGATCGCCAAAGAGGAGGTTTCGAAAG
CGGCGGCAGCCTTCATGCCCAACATCAAGGCAGCTACTTCGAAAGGACGCAGCCGTACCGACAGCGAGACTCCCTACAGC
TCCAGGGAGGGTGTCTATTACAGCACTCAAAGTCACAGCGTTTCACTCCGCCAGCCGCTTTTCAATCTTGGCAGCATTGC
ATCCTGGAAACAGGCCCACGCCGTGAAAGCCAAGAGCGAAGAGCTGTTCCGCAGCGAGCACGCTTCGTTGATTGTCCGGA
CGGCCGAACAGTTTTGCAATGTGCTCTTTTCGCAGGACAACATTACCTTCGCCAGCGCCCAGGTCGAAGCCACAAAAGAG
CAGCTCGAACAGTCGAAGAAACGGTTTGAAAAAGGGTTCGGCACCATCACCGAAATCAACGAGGCGCAGGCAAGCTTCGA
TTTGGCGGTTGCCGATCACGCCGATGCCATTGCCGGTCTGGAAAACAGTTCCCGCGAACTTGAACGCATCACCGGAATTT
ATACCGACGAGCTTTGCCGGCTCGATCCGGAGCGCCTTGTCCTTGCCCGCCCGGAACCGCAGAATGTCGAAGCCTGGGTG
GCGCTTGCAGAGGAGCATAACGGCAGGATCGGTGCGGCGCGTCAGGAGGTTGAAATCACTCGCAAGGAGATCGACAAGAC
CAAAGCTTCACGGTATCCCCAACTCGATCTCTGGGCCGGACGAAGCTATTCGGTCAGCGAGAACAACTATACCATTGGCT
CGACGTATGATACCTGGTCGGTCAACGTGCAATTGAGCATGCCGATCTATACCGGTGGGTACACCAGCGCCGCTGTTCGC
CAGGCTTGGGCTCGCCGTCTCAAGGCGCAGGAAGGGCTTCATTTTCAGGAACGGGAGTCGCTTACCAGAGTCAGGCGTTA
CTATCAGGCCCAGATCAACAGCATCGCGCAGGTCAGGGCATATGAACAGGCAGCCAAATCTGCTGAAATCGCCCTTGAAG
GCACCAGAAAAGGGTTTCAGGCCGGTTTCAGGACTAACGCCGAAGTGCTCGATGCGGTGAAGAAACTGTATGATATCCGG
CGAAGTCTTGCCCGGGCGCGATACCAGTATATCCTCAACAGGCTCATGCTCAGGGATGCGGCCGGATTGCTCAGCGAGTC
TGATCTTGACGGGATCGATGAGTTTTTTGTTCTCGCTGGCTCATAA

Upstream 100 bases:

>100_bases
GATGCAGCGCATGGCGGCATCCCTGAAGGAGGAGTAAGAAATCATGGCGCTAACGATTCGACATTTTCTTATGCGCAGTT
CGGGCAGAAAGAGCAGGAAG

Downstream 100 bases:

>100_bases
GTTTTACCTGTTCTGCCGCTGACCCGGCACTTCTGCCCGTTTTGCCGTTTTATCTCTTTGTGACTGAAAAGAGCTTTCCA
TGGTCATGATCTCTTCGTTC

Product: type I secretion outer membrane protein, TolC family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAFLLVLVQFFLPMAAQAKSLSLKEAYEHALQYDAQYRSAEADTRIAKEEVSKAAAAFMPNIKAATSKGRSRTDSETPYS
SREGVYYSTQSHSVSLRQPLFNLGSIASWKQAHAVKAKSEELFRSEHASLIVRTAEQFCNVLFSQDNITFASAQVEATKE
QLEQSKKRFEKGFGTITEINEAQASFDLAVADHADAIAGLENSSRELERITGIYTDELCRLDPERLVLARPEPQNVEAWV
ALAEEHNGRIGAARQEVEITRKEIDKTKASRYPQLDLWAGRSYSVSENNYTIGSTYDTWSVNVQLSMPIYTGGYTSAAVR
QAWARRLKAQEGLHFQERESLTRVRRYYQAQINSIAQVRAYEQAAKSAEIALEGTRKGFQAGFRTNAEVLDAVKKLYDIR
RSLARARYQYILNRLMLRDAAGLLSESDLDGIDEFFVLAGS

Sequences:

>Translated_441_residues
MAFLLVLVQFFLPMAAQAKSLSLKEAYEHALQYDAQYRSAEADTRIAKEEVSKAAAAFMPNIKAATSKGRSRTDSETPYS
SREGVYYSTQSHSVSLRQPLFNLGSIASWKQAHAVKAKSEELFRSEHASLIVRTAEQFCNVLFSQDNITFASAQVEATKE
QLEQSKKRFEKGFGTITEINEAQASFDLAVADHADAIAGLENSSRELERITGIYTDELCRLDPERLVLARPEPQNVEAWV
ALAEEHNGRIGAARQEVEITRKEIDKTKASRYPQLDLWAGRSYSVSENNYTIGSTYDTWSVNVQLSMPIYTGGYTSAAVR
QAWARRLKAQEGLHFQERESLTRVRRYYQAQINSIAQVRAYEQAAKSAEIALEGTRKGFQAGFRTNAEVLDAVKKLYDIR
RSLARARYQYILNRLMLRDAAGLLSESDLDGIDEFFVLAGS
>Mature_440_residues
AFLLVLVQFFLPMAAQAKSLSLKEAYEHALQYDAQYRSAEADTRIAKEEVSKAAAAFMPNIKAATSKGRSRTDSETPYSS
REGVYYSTQSHSVSLRQPLFNLGSIASWKQAHAVKAKSEELFRSEHASLIVRTAEQFCNVLFSQDNITFASAQVEATKEQ
LEQSKKRFEKGFGTITEINEAQASFDLAVADHADAIAGLENSSRELERITGIYTDELCRLDPERLVLARPEPQNVEAWVA
LAEEHNGRIGAARQEVEITRKEIDKTKASRYPQLDLWAGRSYSVSENNYTIGSTYDTWSVNVQLSMPIYTGGYTSAAVRQ
AWARRLKAQEGLHFQERESLTRVRRYYQAQINSIAQVRAYEQAAKSAEIALEGTRKGFQAGFRTNAEVLDAVKKLYDIRR
SLARARYQYILNRLMLRDAAGLLSESDLDGIDEFFVLAGS

Specific function: Involved in the secretion of alkaline protease [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=422, Percent_Identity=30.5687203791469, Blast_Score=188, Evalue=7e-49,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49538; Mature: 49407

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLLVLVQFFLPMAAQAKSLSLKEAYEHALQYDAQYRSAEADTRIAKEEVSKAAAAFMP
CHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
NIKAATSKGRSRTDSETPYSSREGVYYSTQSHSVSLRQPLFNLGSIASWKQAHAVKAKSE
CCHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
ELFRSEHASLIVRTAEQFCNVLFSQDNITFASAQVEATKEQLEQSKKRFEKGFGTITEIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EAQASFDLAVADHADAIAGLENSSRELERITGIYTDELCRLDPERLVLARPEPQNVEAWV
HHHHHEEEEECCCHHHHHCCCCCHHHHHHHHCCHHHHHHCCCHHHEEEECCCCCCCHHHH
ALAEEHNGRIGAARQEVEITRKEIDKTKASRYPQLDLWAGRSYSVSENNYTIGSTYDTWS
EEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCEEECCCCCEEE
VNVQLSMPIYTGGYTSAAVRQAWARRLKAQEGLHFQERESLTRVRRYYQAQINSIAQVRA
EEEEEEECEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YEQAAKSAEIALEGTRKGFQAGFRTNAEVLDAVKKLYDIRRSLARARYQYILNRLMLRDA
HHHHHHHHHEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLLSESDLDGIDEFFVLAGS
HHHCCCCCCCCCCHHEEEECC
>Mature Secondary Structure 
AFLLVLVQFFLPMAAQAKSLSLKEAYEHALQYDAQYRSAEADTRIAKEEVSKAAAAFMP
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
NIKAATSKGRSRTDSETPYSSREGVYYSTQSHSVSLRQPLFNLGSIASWKQAHAVKAKSE
CCHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
ELFRSEHASLIVRTAEQFCNVLFSQDNITFASAQVEATKEQLEQSKKRFEKGFGTITEIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EAQASFDLAVADHADAIAGLENSSRELERITGIYTDELCRLDPERLVLARPEPQNVEAWV
HHHHHEEEEECCCHHHHHCCCCCHHHHHHHHCCHHHHHHCCCHHHEEEECCCCCCCHHHH
ALAEEHNGRIGAARQEVEITRKEIDKTKASRYPQLDLWAGRSYSVSENNYTIGSTYDTWS
EEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCEEECCCCCEEE
VNVQLSMPIYTGGYTSAAVRQAWARRLKAQEGLHFQERESLTRVRRYYQAQINSIAQVRA
EEEEEEECEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YEQAAKSAEIALEGTRKGFQAGFRTNAEVLDAVKKLYDIRRSLARARYQYILNRLMLRDA
HHHHHHHHHEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLLSESDLDGIDEFFVLAGS
HHHCCCCCCCCCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1427098; 10984043 [H]