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The map label for this gene is nadE

Identifier: 193212251

GI number: 193212251

Start: 627709

End: 628542

Strand: Direct

Name: nadE

Synonym: Cpar_0584

Alternate gene names: 193212251

Gene position: 627709-628542 (Clockwise)

Preceding gene: 193212250

Following gene: 193212260

Centisome position: 27.42

GC content: 61.75

Gene sequence:

>834_bases
ATGGAACCTCAAGACCTCCATCTCGATTACGGCCTGGTCGAATCGATTCTGATCCCTTTCATTCGCAACGAAATCAGGAA
GTTCGGGTTCCGGTCGGTGGTGCTCGGCCTTTCCGGTGGCATCGATTCGGCGGTGGTGTGCGAGCTGGCTGCGCGGTCGC
TCGGTGCCGATAACGTGCTGGCACTGCTGATGCCCTACAAAACCAGCAGCCGAGAAAGCCTGGAACACGCTCAGCTCATG
GTGGACCGGCTCGGCATTCGCGCCGAGACCATGCCTGTGACCGGGGTGGTCGATGCCTTTTTCGAGACGCGTCCGGACGC
CAGCCGCCTGCGGCGCGGCAACGTCATGGCGCGCTCCCGGATGCTTTGCCTGTACGACGTGTCGGCGCGGGACGGCTGCC
TGGTGCTCGGCACGAGCAACAAAACCGAGCTGATGCTGGGCTACGGCACCATGTTCGGCGACATGGCTTCGGCGGTCAAC
CCCATCGGCGACCTTTACAAAACCCAGCTCTGGGGCCTGGCCCGGCATCTTGGTGTCCCCTCGGAGCTGATCGACAAACA
GCCCTCTGCCGACCTCTGGGAGGGCCAGAGCGACGAGGCCGATCTCGGCTTCAGCTACGAGGAGGTTGACCAGCTTCTGT
ACATGATGCTTGAAGAGCGCATGGAGCGCGACGCCATTTTGGCTGAAGGCATTGCCCCTGCCTTTTACGACCGGGTACGG
CAGATGGTGGTGCGCAACCAGTACAAGCGCATGATGCCCGTTATTGCCAAACTCTCCAGCCGCACGCCGGGCATCGATTT
CCGCTATGCCCGCGACTGGCAGGAAATGAAGTGA

Upstream 100 bases:

>100_bases
TCGAAAAAGTCCGGCGTCCTCATGGCCACGGCGACCTTTATTTCGTTTATGCTCATCGGCGGGCTGATCCGTTTTTTGAC
CTCTATATGACGCTCCCTTT

Downstream 100 bases:

>100_bases
GGGGACTTTGATCTTCAGTCGATCTCAAGTCCCCTGACTCAAAGATTGGGGCTCAAAACGACCGCAGCACCGTATCCACC
AGATAATGCTTGTCGTCCCT

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MEPQDLHLDYGLVESILIPFIRNEIRKFGFRSVVLGLSGGIDSAVVCELAARSLGADNVLALLMPYKTSSRESLEHAQLM
VDRLGIRAETMPVTGVVDAFFETRPDASRLRRGNVMARSRMLCLYDVSARDGCLVLGTSNKTELMLGYGTMFGDMASAVN
PIGDLYKTQLWGLARHLGVPSELIDKQPSADLWEGQSDEADLGFSYEEVDQLLYMMLEERMERDAILAEGIAPAFYDRVR
QMVVRNQYKRMMPVIAKLSSRTPGIDFRYARDWQEMK

Sequences:

>Translated_277_residues
MEPQDLHLDYGLVESILIPFIRNEIRKFGFRSVVLGLSGGIDSAVVCELAARSLGADNVLALLMPYKTSSRESLEHAQLM
VDRLGIRAETMPVTGVVDAFFETRPDASRLRRGNVMARSRMLCLYDVSARDGCLVLGTSNKTELMLGYGTMFGDMASAVN
PIGDLYKTQLWGLARHLGVPSELIDKQPSADLWEGQSDEADLGFSYEEVDQLLYMMLEERMERDAILAEGIAPAFYDRVR
QMVVRNQYKRMMPVIAKLSSRTPGIDFRYARDWQEMK
>Mature_277_residues
MEPQDLHLDYGLVESILIPFIRNEIRKFGFRSVVLGLSGGIDSAVVCELAARSLGADNVLALLMPYKTSSRESLEHAQLM
VDRLGIRAETMPVTGVVDAFFETRPDASRLRRGNVMARSRMLCLYDVSARDGCLVLGTSNKTELMLGYGTMFGDMASAVN
PIGDLYKTQLWGLARHLGVPSELIDKQPSADLWEGQSDEADLGFSYEEVDQLLYMMLEERMERDAILAEGIAPAFYDRVR
QMVVRNQYKRMMPVIAKLSSRTPGIDFRYARDWQEMK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=217, Percent_Identity=29.9539170506912, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_CHLP8 (B3QM51)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001998204.1
- GeneID:   6419502
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0584
- HOGENOM:   HBG351567
- OMA:   FRYARDW
- ProtClustDB:   PRK13980
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 31189; Mature: 31189

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPQDLHLDYGLVESILIPFIRNEIRKFGFRSVVLGLSGGIDSAVVCELAARSLGADNVL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCE
ALLMPYKTSSRESLEHAQLMVDRLGIRAETMPVTGVVDAFFETRPDASRLRRGNVMARSR
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHC
MLCLYDVSARDGCLVLGTSNKTELMLGYGTMFGDMASAVNPIGDLYKTQLWGLARHLGVP
EEEEEECCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SELIDKQPSADLWEGQSDEADLGFSYEEVDQLLYMMLEERMERDAILAEGIAPAFYDRVR
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QMVVRNQYKRMMPVIAKLSSRTPGIDFRYARDWQEMK
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHCC
>Mature Secondary Structure
MEPQDLHLDYGLVESILIPFIRNEIRKFGFRSVVLGLSGGIDSAVVCELAARSLGADNVL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCE
ALLMPYKTSSRESLEHAQLMVDRLGIRAETMPVTGVVDAFFETRPDASRLRRGNVMARSR
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHC
MLCLYDVSARDGCLVLGTSNKTELMLGYGTMFGDMASAVNPIGDLYKTQLWGLARHLGVP
EEEEEECCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SELIDKQPSADLWEGQSDEADLGFSYEEVDQLLYMMLEERMERDAILAEGIAPAFYDRVR
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QMVVRNQYKRMMPVIAKLSSRTPGIDFRYARDWQEMK
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA