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The map label for this gene is carB [H]

Identifier: 193212186

GI number: 193212186

Start: 555451

End: 556935

Strand: Direct

Name: carB [H]

Synonym: Cpar_0517

Alternate gene names: 193212186

Gene position: 555451-556935 (Clockwise)

Preceding gene: 193212185

Following gene: 193212187

Centisome position: 24.26

GC content: 59.66

Gene sequence:

>1485_bases
GTGCCAAAACGCGAGGACATCAAGTCCATATTGGTGATTGGAGCCGGTCCCATCGTTATCGGACAGGCCTGTGAATTCGA
TTATTCGGGCACCCAGGCGTGCCGGGCTCTCAAGGAGGACGGCTACCGGGTCATTCTGGTCAACAGCAACCCGGCCACCA
TCATGACCGACATCGAGCTGGCCGATGCGACCTACATCGAACCGATCACGCCGGAGTATGTCCGCAAGATTATCGAGAAG
GAAAAGCCGGACGCGTTGCTTCCGACCATGGGCGGTCAGACCGCGCTGAACACTGCGGTCAAGCTGGCCGAGTCGGGCGT
ACTGGAGCGCCACGGCGTCGAGCTGATCGGTGCGAAGCTCCGCGCGATTCGCAAGGCCGAGAACCGCGAATTCTTCGGCG
ATGCGATGCGCAAGCTCGGCCTCGAAATGGCCAAGGGTTTCTTCGTTCGCAACGAGAAGGAGGCCAAGGAGGCGCTCGAT
GAAATCGGCTTGCCGATCGTCATTCGCCCCTCCTTCACGCTTGGCGGCACGGGTGGCGGGTTTGCCGAGACCAAGGCTGA
CTACTACGATGTGGTTCGCCGTGGCCTTGCCGAAAGCCCGATTGGCGAAGTGCTGGTCGAGGAGAGCCTGATCGGCTGGA
AGGAGTACGAACTTGAAGTGATCCGTGATTTGGCGGACAACGTCATCATCGTCTGCTCCATCGAGAACGTCGATCCGATG
GGCGTGCATACCGGCGACAGCATCACAGTCGCTCCGGCGCAGACGCTCACCGACCGTCAGTATCAGGAGTTGCGTGACGC
CTCGATAAAGATCATCCGCGAAATCGGCGTCGAGACCGGCGGCAGCAATATCCAGTTCTCGATCAATCCGAAGGATGGCC
GTATCGTCATCATCGAAATGAACCCGCGCGTGTCGAGAAGCTCCGCGCTCGCCTCGAAGGCGACCGGTTTCCCCATCGCG
AAAGTGGCTGCCAAGCTCGCCGTGGGCTACACGCTCGACGAGATCACCAACGACATCACCAAAACCACCCCGGCAAGCTT
CGAGCCCTCGATCGACTACACCGTCGTGAAGGTGCCGCGCTGGGACTTCGAGAAGTTCAAGAATGTCGATTCGCGCCTCG
GCGTGCAGATGAAGTCGGTCGGCGAGGTGATGGCTTTCGGACGCAATTTCCGCGAGGCGTTGCAGAAGTCGCTGCGCGGC
CTCGAAATCGGACGCGCCGGTCTTGGCTGCGATGGCAAGGATGTGATGAACGTCATCGACATGACCCAGCAGCAGCGTCA
GTTCGCCAAGGAGGATCTGCTTGAAAAGATCAAGATCCCGAAGGCCGACCGGATGTTCTACCTTCGCTACGCCTTCCAGG
CCGGGGCAACCGTCGAGGAGATCCATCAGGCAACCAGCATCGATCCGTGGTTCCTTGACAACATCCGCCAGATTGTCGAG
TTCGAGGATGAGCTCCGCAAGATGGCAGCTGAAAGCGAGGCATGA

Upstream 100 bases:

>100_bases
CGACAGGAGGCCTTTGAGCCTCCAAAGCCTCACAGAACACCGGAAAATTTTGTACATTACGCAACGATAGATTTACCTCA
AGACTTAAAGCGGAAAACAC

Downstream 100 bases:

>100_bases
CGTACCTCACCCGGATTCTTGAGACCAAGGCCGAAGAGGTCGCCGAACTCAAAAAGCAGGACCCGGAACGCCGCTACCAC
GAAGCGTGCGGCGATCTTCC

Product: carbamoyl-phosphate synthase L chain ATP-binding

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MPKREDIKSILVIGAGPIVIGQACEFDYSGTQACRALKEDGYRVILVNSNPATIMTDIELADATYIEPITPEYVRKIIEK
EKPDALLPTMGGQTALNTAVKLAESGVLERHGVELIGAKLRAIRKAENREFFGDAMRKLGLEMAKGFFVRNEKEAKEALD
EIGLPIVIRPSFTLGGTGGGFAETKADYYDVVRRGLAESPIGEVLVEESLIGWKEYELEVIRDLADNVIIVCSIENVDPM
GVHTGDSITVAPAQTLTDRQYQELRDASIKIIREIGVETGGSNIQFSINPKDGRIVIIEMNPRVSRSSALASKATGFPIA
KVAAKLAVGYTLDEITNDITKTTPASFEPSIDYTVVKVPRWDFEKFKNVDSRLGVQMKSVGEVMAFGRNFREALQKSLRG
LEIGRAGLGCDGKDVMNVIDMTQQQRQFAKEDLLEKIKIPKADRMFYLRYAFQAGATVEEIHQATSIDPWFLDNIRQIVE
FEDELRKMAAESEA

Sequences:

>Translated_494_residues
MPKREDIKSILVIGAGPIVIGQACEFDYSGTQACRALKEDGYRVILVNSNPATIMTDIELADATYIEPITPEYVRKIIEK
EKPDALLPTMGGQTALNTAVKLAESGVLERHGVELIGAKLRAIRKAENREFFGDAMRKLGLEMAKGFFVRNEKEAKEALD
EIGLPIVIRPSFTLGGTGGGFAETKADYYDVVRRGLAESPIGEVLVEESLIGWKEYELEVIRDLADNVIIVCSIENVDPM
GVHTGDSITVAPAQTLTDRQYQELRDASIKIIREIGVETGGSNIQFSINPKDGRIVIIEMNPRVSRSSALASKATGFPIA
KVAAKLAVGYTLDEITNDITKTTPASFEPSIDYTVVKVPRWDFEKFKNVDSRLGVQMKSVGEVMAFGRNFREALQKSLRG
LEIGRAGLGCDGKDVMNVIDMTQQQRQFAKEDLLEKIKIPKADRMFYLRYAFQAGATVEEIHQATSIDPWFLDNIRQIVE
FEDELRKMAAESEA
>Mature_493_residues
PKREDIKSILVIGAGPIVIGQACEFDYSGTQACRALKEDGYRVILVNSNPATIMTDIELADATYIEPITPEYVRKIIEKE
KPDALLPTMGGQTALNTAVKLAESGVLERHGVELIGAKLRAIRKAENREFFGDAMRKLGLEMAKGFFVRNEKEAKEALDE
IGLPIVIRPSFTLGGTGGGFAETKADYYDVVRRGLAESPIGEVLVEESLIGWKEYELEVIRDLADNVIIVCSIENVDPMG
VHTGDSITVAPAQTLTDRQYQELRDASIKIIREIGVETGGSNIQFSINPKDGRIVIIEMNPRVSRSSALASKATGFPIAK
VAAKLAVGYTLDEITNDITKTTPASFEPSIDYTVVKVPRWDFEKFKNVDSRLGVQMKSVGEVMAFGRNFREALQKSLRGL
EIGRAGLGCDGKDVMNVIDMTQQQRQFAKEDLLEKIKIPKADRMFYLRYAFQAGATVEEIHQATSIDPWFLDNIRQIVEF
EDELRKMAAESEA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ATP-grasp domains [H]

Homologues:

Organism=Homo sapiens, GI21361331, Length=492, Percent_Identity=42.2764227642276, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI169790915, Length=492, Percent_Identity=42.2764227642276, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI18105007, Length=482, Percent_Identity=44.3983402489627, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI170295797, Length=459, Percent_Identity=41.6122004357298, Blast_Score=382, Evalue=1e-106,
Organism=Escherichia coli, GI1786216, Length=486, Percent_Identity=62.5514403292181, Blast_Score=613, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI193204318, Length=481, Percent_Identity=45.5301455301455, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322569, Length=490, Percent_Identity=45.5102040816327, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322331, Length=491, Percent_Identity=43.9918533604888, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24642586, Length=494, Percent_Identity=43.5222672064777, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI45555749, Length=494, Percent_Identity=43.5222672064777, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24651757, Length=367, Percent_Identity=23.9782016348774, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006275
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; PF02142 MGS [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 54690; Mature: 54559

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50975 ATP_GRASP ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKREDIKSILVIGAGPIVIGQACEFDYSGTQACRALKEDGYRVILVNSNPATIMTDIEL
CCCHHHHCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEE
ADATYIEPITPEYVRKIIEKEKPDALLPTMGGQTALNTAVKLAESGVLERHGVELIGAKL
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHH
RAIRKAENREFFGDAMRKLGLEMAKGFFVRNEKEAKEALDEIGLPIVIRPSFTLGGTGGG
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCEEECCCCCC
FAETKADYYDVVRRGLAESPIGEVLVEESLIGWKEYELEVIRDLADNVIIVCSIENVDPM
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEECCCCCC
GVHTGDSITVAPAQTLTDRQYQELRDASIKIIREIGVETGGSNIQFSINPKDGRIVIIEM
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEC
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDEITNDITKTTPASFEPSIDYTVVKVPR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCC
WDFEKFKNVDSRLGVQMKSVGEVMAFGRNFREALQKSLRGLEIGRAGLGCDGKDVMNVID
CCHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
MTQQQRQFAKEDLLEKIKIPKADRMFYLRYAFQAGATVEEIHQATSIDPWFLDNIRQIVE
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCCHHHHHHHCCCCHHHHHHHHHHHH
FEDELRKMAAESEA
HHHHHHHHHHCCCC
>Mature Secondary Structure 
PKREDIKSILVIGAGPIVIGQACEFDYSGTQACRALKEDGYRVILVNSNPATIMTDIEL
CCHHHHCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEE
ADATYIEPITPEYVRKIIEKEKPDALLPTMGGQTALNTAVKLAESGVLERHGVELIGAKL
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHH
RAIRKAENREFFGDAMRKLGLEMAKGFFVRNEKEAKEALDEIGLPIVIRPSFTLGGTGGG
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCEEECCCCCC
FAETKADYYDVVRRGLAESPIGEVLVEESLIGWKEYELEVIRDLADNVIIVCSIENVDPM
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEECCCCCC
GVHTGDSITVAPAQTLTDRQYQELRDASIKIIREIGVETGGSNIQFSINPKDGRIVIIEM
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEC
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDEITNDITKTTPASFEPSIDYTVVKVPR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCC
WDFEKFKNVDSRLGVQMKSVGEVMAFGRNFREALQKSLRGLEIGRAGLGCDGKDVMNVID
CCHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
MTQQQRQFAKEDLLEKIKIPKADRMFYLRYAFQAGATVEEIHQATSIDPWFLDNIRQIVE
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCCHHHHHHHCCCCHHHHHHHHHHHH
FEDELRKMAAESEA
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]