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The map label for this gene is purD [H]

Identifier: 193212184

GI number: 193212184

Start: 553533

End: 554810

Strand: Direct

Name: purD [H]

Synonym: Cpar_0515

Alternate gene names: 193212184

Gene position: 553533-554810 (Clockwise)

Preceding gene: 193212181

Following gene: 193212185

Centisome position: 24.18

GC content: 60.95

Gene sequence:

>1278_bases
ATGAATGTTCTGATTATCGGAAGCGGCGCCAGGGAGCATGCTCTGGCTTGGGCCGTTGCGCGCAGTTCGAAAGTTTCAAC
CGTGCTGGTGGCTCCGGGCAACGGAGGCACGGCCACGATGGGCGGCAAGGTTCGCAACGTCGCGATCAAGGCGACTGACA
TCGACGCACTGCTCGATCTGGTTGCGAAAGAGTCGATTGGTCTGACGGTGGTCGGCCCTGAGCAGCCGCTCGAAGTTGGC
ATCGTGAACCGCTTTCGCGATGCGGGGCTGAAGATCGTCGGCCCGACCGCCGAGGCAGCGCAGCTGGAGACGAGCAAGGT
GTTCGCCAAAGACTTTATGAAGCGTCATGGCATTCCGACCGCCGGGTATGAAGTGTTCCGCGACCACGCGTCCGCGAAAA
GCTATCTTGAAGCGTGTGGCTCGTTCCCGCAGGTGATCAAGGCCAGTGGTCTGTGTGCCGGAAAAGGCGTCGTCGTCGCG
ATGACTCGCGAAGAGGCGCTGGAGGCAATCCACGATTTCTTCGAGTCCCGTGTCTTTGGCGATGCGGCTGACGAGGTGGT
GATCGAAGCGTTCCTGACCGGTCAGGAGGCGAGTGTTTTCGCGCTGACCGATGGTGAGAACTACCAGCTGTTCCTAGCTG
CACAGGATCACAAGCGCATCGGCGAAGGTGATACCGGCAAGAATACCGGCGGCATGGGTGCCTACGCCCCGGCTCCGCTG
GTGACGCCGGAGGTGATGCGGCGGGTCGAGGAAGAGGTCATCAAACCGACGCTTGCCGGCATGAAGGCAGACGGCTACGC
CTACACCGGTTTCCTCTACGTTGGCCTCATGATCGACAACGGCGAGCCCTCGGTGGTCGAGTACAACGTCCGCCTCGGTG
ATCCCGAAACACAGGTGGTGTTGCCGATGCTGAAGAGCGACCTGTTCGACGCGCTGCTGGCAAGCGTCGAGGGCGGGCTT
GAAGCCGTGCCGTTCGAGATGCAGAGCGGAGCCGCCGCCACGGTCGTTATGGCCTCGAAGGGCTATCCCGACGGATACGA
AACCGGCAAGGCGATCTCGATCGATCCTTCGGTGAACGATATGGATGGAGTGCTTGTTTTCCATGCCGGTACGCGCCAGG
ATGGTGATGTGCTTGTAACTTCGGGTGGTCGCGTGCTCTCGGTGACAGCCTCCGGTGCGTCACTTAAAGAGGCGCTCGAT
CAAGCCTATCGCGGCGTAGCAGCTATCAATTTCGAGGGGGCAGTCTATCGCCGGGACATCGGCGCAAAGGCGCTTTGA

Upstream 100 bases:

>100_bases
TATATACATCCAGCGTTCAACCGATCCGGGCGGACGGCATCAATTTAACAATCCACGCCGACAAACCACAGGTTTTGAGG
CCATTGTTCCATACCTGCAC

Downstream 100 bases:

>100_bases
CGTATCAGCTATCTTATTCTCGCATTGGTCAGTAATGAAATTACCTCGTCGCAAATTTGAAATCATACAGGAGCACGCCA
TCAGGGATCTTCCCTACGAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNVLIIGSGAREHALAWAVARSSKVSTVLVAPGNGGTATMGGKVRNVAIKATDIDALLDLVAKESIGLTVVGPEQPLEVG
IVNRFRDAGLKIVGPTAEAAQLETSKVFAKDFMKRHGIPTAGYEVFRDHASAKSYLEACGSFPQVIKASGLCAGKGVVVA
MTREEALEAIHDFFESRVFGDAADEVVIEAFLTGQEASVFALTDGENYQLFLAAQDHKRIGEGDTGKNTGGMGAYAPAPL
VTPEVMRRVEEEVIKPTLAGMKADGYAYTGFLYVGLMIDNGEPSVVEYNVRLGDPETQVVLPMLKSDLFDALLASVEGGL
EAVPFEMQSGAAATVVMASKGYPDGYETGKAISIDPSVNDMDGVLVFHAGTRQDGDVLVTSGGRVLSVTASGASLKEALD
QAYRGVAAINFEGAVYRRDIGAKAL

Sequences:

>Translated_425_residues
MNVLIIGSGAREHALAWAVARSSKVSTVLVAPGNGGTATMGGKVRNVAIKATDIDALLDLVAKESIGLTVVGPEQPLEVG
IVNRFRDAGLKIVGPTAEAAQLETSKVFAKDFMKRHGIPTAGYEVFRDHASAKSYLEACGSFPQVIKASGLCAGKGVVVA
MTREEALEAIHDFFESRVFGDAADEVVIEAFLTGQEASVFALTDGENYQLFLAAQDHKRIGEGDTGKNTGGMGAYAPAPL
VTPEVMRRVEEEVIKPTLAGMKADGYAYTGFLYVGLMIDNGEPSVVEYNVRLGDPETQVVLPMLKSDLFDALLASVEGGL
EAVPFEMQSGAAATVVMASKGYPDGYETGKAISIDPSVNDMDGVLVFHAGTRQDGDVLVTSGGRVLSVTASGASLKEALD
QAYRGVAAINFEGAVYRRDIGAKAL
>Mature_425_residues
MNVLIIGSGAREHALAWAVARSSKVSTVLVAPGNGGTATMGGKVRNVAIKATDIDALLDLVAKESIGLTVVGPEQPLEVG
IVNRFRDAGLKIVGPTAEAAQLETSKVFAKDFMKRHGIPTAGYEVFRDHASAKSYLEACGSFPQVIKASGLCAGKGVVVA
MTREEALEAIHDFFESRVFGDAADEVVIEAFLTGQEASVFALTDGENYQLFLAAQDHKRIGEGDTGKNTGGMGAYAPAPL
VTPEVMRRVEEEVIKPTLAGMKADGYAYTGFLYVGLMIDNGEPSVVEYNVRLGDPETQVVLPMLKSDLFDALLASVEGGL
EAVPFEMQSGAAATVVMASKGYPDGYETGKAISIDPSVNDMDGVLVFHAGTRQDGDVLVTSGGRVLSVTASGASLKEALD
QAYRGVAAINFEGAVYRRDIGAKAL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=49.2957746478873, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=49.4117647058824, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=49.4117647058824, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=49.4117647058824, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=53.1615925058548, Blast_Score=415, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=39.7647058823529, Blast_Score=293, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=42.4719101123596, Blast_Score=345, Evalue=7e-96,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=43.1554524361949, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=43.1554524361949, Blast_Score=323, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44613; Mature: 44613

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLIIGSGAREHALAWAVARSSKVSTVLVAPGNGGTATMGGKVRNVAIKATDIDALLDL
CEEEEEECCCCHHHHEEEEECCCCCEEEEEECCCCCCEECCCEEEEEEEEECCHHHHHHH
VAKESIGLTVVGPEQPLEVGIVNRFRDAGLKIVGPTAEAAQLETSKVFAKDFMKRHGIPT
HHHCCCCEEEECCCCCCEEHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCC
AGYEVFRDHASAKSYLEACGSFPQVIKASGLCAGKGVVVAMTREEALEAIHDFFESRVFG
CHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
DAADEVVIEAFLTGQEASVFALTDGENYQLFLAAQDHKRIGEGDTGKNTGGMGAYAPAPL
CCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
VTPEVMRRVEEEVIKPTLAGMKADGYAYTGFLYVGLMIDNGEPSVVEYNVRLGDPETQVV
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECCCCCEEEEEEEECCCCCEEE
LPMLKSDLFDALLASVEGGLEAVPFEMQSGAAATVVMASKGYPDGYETGKAISIDPSVND
HHHHHHHHHHHHHHHHCCCCEECCEECCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCC
MDGVLVFHAGTRQDGDVLVTSGGRVLSVTASGASLKEALDQAYRGVAAINFEGAVYRRDI
CCCEEEEEECCCCCCCEEEECCCEEEEEEECCHHHHHHHHHHHCCEEEEEECCEEEEECC
GAKAL
CCCCC
>Mature Secondary Structure
MNVLIIGSGAREHALAWAVARSSKVSTVLVAPGNGGTATMGGKVRNVAIKATDIDALLDL
CEEEEEECCCCHHHHEEEEECCCCCEEEEEECCCCCCEECCCEEEEEEEEECCHHHHHHH
VAKESIGLTVVGPEQPLEVGIVNRFRDAGLKIVGPTAEAAQLETSKVFAKDFMKRHGIPT
HHHCCCCEEEECCCCCCEEHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCC
AGYEVFRDHASAKSYLEACGSFPQVIKASGLCAGKGVVVAMTREEALEAIHDFFESRVFG
CHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
DAADEVVIEAFLTGQEASVFALTDGENYQLFLAAQDHKRIGEGDTGKNTGGMGAYAPAPL
CCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
VTPEVMRRVEEEVIKPTLAGMKADGYAYTGFLYVGLMIDNGEPSVVEYNVRLGDPETQVV
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECCCCCEEEEEEEECCCCCEEE
LPMLKSDLFDALLASVEGGLEAVPFEMQSGAAATVVMASKGYPDGYETGKAISIDPSVND
HHHHHHHHHHHHHHHHCCCCEECCEECCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCC
MDGVLVFHAGTRQDGDVLVTSGGRVLSVTASGASLKEALDQAYRGVAAINFEGAVYRRDI
CCCEEEEEECCCCCCCEEEECCCEEEEEEECCHHHHHHHHHHHCCEEEEEECCEEEEECC
GAKAL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]