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The map label for this gene is relA [H]

Identifier: 193212082

GI number: 193212082

Start: 445240

End: 447435

Strand: Direct

Name: relA [H]

Synonym: Cpar_0412

Alternate gene names: 193212082

Gene position: 445240-447435 (Clockwise)

Preceding gene: 193212081

Following gene: 193212083

Centisome position: 19.45

GC content: 56.47

Gene sequence:

>2196_bases
ATGCTGGCCCAGATAGAACAGGAACATTACCAGAAACTGCACGAAATTCTTCGGCTCTGCCGGGCGAATCTCAAGAACTA
CGACGAGTCGCTTATCCAGCGTGCATTTTTCATGTGCTATCGCGCTCATGAGGGGGAGAAACGCGCTTCCGGCGAGCCGT
TCTTCTACCATCCGGTGGAGGTTGCCAAGCTGCTGGTGACCGAGCTTCCGCTTGATGGCGTTTCCGTTGCGGCGGCGCTG
CTGCACGATGTCATCGAGGACAGCGGCTATACCTACGAAGACATCGCTGCCGAGCTGGGCACTGAAGTAGCCGACATCGT
CGAGGGGCTCACCAAGATCTCCGGCATCATGGTCAATCGTGAGACCACCGAGGCCGAAGGGTTCCGCAAGATGCTGCTCT
CGATGGTCAAGGATATCCGCGTCATCCTCATCAAGTTCTGCGACCGACTGCACAACATGCGCACGCTCGACTCCCTGCCG
GAGCATCGCCGCTTGCGCATGGCGCTCGAAACGCGCGACATCTACGCGCCGCTCGCCCACCGGTTCGGTCTCGGTAAAAT
GAAGGTCGATCTGGAGAATCTCGCCTTCAAGTACATCGACCCCAAGATGTACGATTACCTGCTCAAGAAGGTGCGCCTGA
GCCGCAACGAGCGCATGGCCTATCTGAACAAGATGATCGCGCCGATCAAGGATGACCTCGAAAAACAGGGGTTCAACGTG
GAAGTGCAGGGCCGCGCCAAGCATCTTTTTTCGATCTACAACAAGATGCGCCTGAAGAACAAGCAGTTCGATGATATCCA
CGATCTGTATGGCATTCGCGTCATCATCGATACGGACAAGCTGTCTGACTGTTTTGCGGTGTACGGCTACATCACGCAGA
AGTATCCGCCAATTCCGCAGCACTTCAAGGATTACATCTCCATTCCGAAGCACAACGGCTATCAGTCGCTGCACTCGGCG
ATTATCGGCCCCAAAGGCCACGTCATCGAGTTGCAGATCCGCACGCGCCGGATGCACGAATTTGCCGAGCTTGGTGTCGC
GGCTCACTGGCGCTACAAGGAGAAGATTTCCAAGGATGACGCCGCCGTCGATTCGTTCCTCAAGTGGGCGCGCGAGCTGA
TCAAGGATGCCGACACGGCCTCCGCCTTCATGGAGGGGTTCAAGCTCAACCTCTACCACGACGAGATTTACGTTTTCACC
CCGAAGGGCGACATGAAGATTCTGCCCGCCGGGGCAACGCCGATCGACTTCGCCTATGCGATTCACACCGAAATCGGCAA
CGGCTGCATCGGCGCAAAGGTTAACGGCAAGATTGTGCGTCTGAACGCCGAGTTGCGGTCCGGTGACCGGGTCGAGGTGA
TTACCTCCAAGAACCAGAAGCCCAAGCCGGACTGGCTCAAGATTGTGGTGACCCATCGTGCCAAGCTCAAGATTCGCGCG
GCCATCAACGAAGAGCGGCGCAAGGAGATCGAGAAGGGGCGCAACATCTTTGACAAGATGCTCACTGGTACCAAGCGGCT
GTTTACCGAAAACGATGCTATCCGCGCCATCCGCAAGCATGGCATCAAAACCCCTGCCGACCTGTTCAGTGCCTTGGCCA
ACCAGCAGATCAGCAGCGAAGAGGTGATGGAGAGCATCACCCGTCCGCACGGCAAGGGCGCCGAACATGAAGGCGACGTT
CAGGGCAAGGCTCCGCACAAGGAGTTCGCCGAGATCGCCCGTGAAGTGCAGGAACGCCCCACCTCTCACAAGGATGAGGT
GACCATTGCCGGAATGAACAACATCGCCTACTCTTATGCGAAGTGCTGCAATCCGGTGCCCGGCGACGACATCATCGGCT
TTGTGACCACCGAAGGCGCGGTCAAGATCCACCGCAAGAACTGCTTCAACGTCACCAACGAAAACTCCGTTAAAAGTGAG
CGGATCGTTTCGGTCGCCTGGAACCGCAAGCTCAACACTGAATTCCTGGCGGGTATCCGCATCGTCGGTGAAGACAAGGT
TGGCATGACCAACCAGATTACCGGTGTCATCTCCAAATTCGACACCAATATCCGCACTATCACACTGCACGCAAAAGACG
GCATTTTTGTGTGCAACCTCATGATCTTCGTCAAGAACACCGACAAGCTCACGACGCTGATGGACAAGCTGAAAAAGGTG
CAGGGCGTGTTTACGGTGGAGCGTATGTCGGCGTAG

Upstream 100 bases:

>100_bases
CAGGCTGAAATGGCATTGACACTGTACGGCATATTTCCTATATAGATTGATAGCACTAAAATGTAGCCGTTAACAATATT
TACCGGAAAGGTGAACCTCC

Downstream 100 bases:

>100_bases
GGGATAGTTGACAATGGATAATTGATAATTAATTATCCATCAGGCGTGATTTGTAGGGGCGGGCCAGTGTGTCCGCCCTT
TTGCGTTATGTCATGGGCAA

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MLAQIEQEHYQKLHEILRLCRANLKNYDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVAKLLVTELPLDGVSVAAAL
LHDVIEDSGYTYEDIAAELGTEVADIVEGLTKISGIMVNRETTEAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLDSLP
EHRRLRMALETRDIYAPLAHRFGLGKMKVDLENLAFKYIDPKMYDYLLKKVRLSRNERMAYLNKMIAPIKDDLEKQGFNV
EVQGRAKHLFSIYNKMRLKNKQFDDIHDLYGIRVIIDTDKLSDCFAVYGYITQKYPPIPQHFKDYISIPKHNGYQSLHSA
IIGPKGHVIELQIRTRRMHEFAELGVAAHWRYKEKISKDDAAVDSFLKWARELIKDADTASAFMEGFKLNLYHDEIYVFT
PKGDMKILPAGATPIDFAYAIHTEIGNGCIGAKVNGKIVRLNAELRSGDRVEVITSKNQKPKPDWLKIVVTHRAKLKIRA
AINEERRKEIEKGRNIFDKMLTGTKRLFTENDAIRAIRKHGIKTPADLFSALANQQISSEEVMESITRPHGKGAEHEGDV
QGKAPHKEFAEIAREVQERPTSHKDEVTIAGMNNIAYSYAKCCNPVPGDDIIGFVTTEGAVKIHRKNCFNVTNENSVKSE
RIVSVAWNRKLNTEFLAGIRIVGEDKVGMTNQITGVISKFDTNIRTITLHAKDGIFVCNLMIFVKNTDKLTTLMDKLKKV
QGVFTVERMSA

Sequences:

>Translated_731_residues
MLAQIEQEHYQKLHEILRLCRANLKNYDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVAKLLVTELPLDGVSVAAAL
LHDVIEDSGYTYEDIAAELGTEVADIVEGLTKISGIMVNRETTEAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLDSLP
EHRRLRMALETRDIYAPLAHRFGLGKMKVDLENLAFKYIDPKMYDYLLKKVRLSRNERMAYLNKMIAPIKDDLEKQGFNV
EVQGRAKHLFSIYNKMRLKNKQFDDIHDLYGIRVIIDTDKLSDCFAVYGYITQKYPPIPQHFKDYISIPKHNGYQSLHSA
IIGPKGHVIELQIRTRRMHEFAELGVAAHWRYKEKISKDDAAVDSFLKWARELIKDADTASAFMEGFKLNLYHDEIYVFT
PKGDMKILPAGATPIDFAYAIHTEIGNGCIGAKVNGKIVRLNAELRSGDRVEVITSKNQKPKPDWLKIVVTHRAKLKIRA
AINEERRKEIEKGRNIFDKMLTGTKRLFTENDAIRAIRKHGIKTPADLFSALANQQISSEEVMESITRPHGKGAEHEGDV
QGKAPHKEFAEIAREVQERPTSHKDEVTIAGMNNIAYSYAKCCNPVPGDDIIGFVTTEGAVKIHRKNCFNVTNENSVKSE
RIVSVAWNRKLNTEFLAGIRIVGEDKVGMTNQITGVISKFDTNIRTITLHAKDGIFVCNLMIFVKNTDKLTTLMDKLKKV
QGVFTVERMSA
>Mature_731_residues
MLAQIEQEHYQKLHEILRLCRANLKNYDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVAKLLVTELPLDGVSVAAAL
LHDVIEDSGYTYEDIAAELGTEVADIVEGLTKISGIMVNRETTEAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLDSLP
EHRRLRMALETRDIYAPLAHRFGLGKMKVDLENLAFKYIDPKMYDYLLKKVRLSRNERMAYLNKMIAPIKDDLEKQGFNV
EVQGRAKHLFSIYNKMRLKNKQFDDIHDLYGIRVIIDTDKLSDCFAVYGYITQKYPPIPQHFKDYISIPKHNGYQSLHSA
IIGPKGHVIELQIRTRRMHEFAELGVAAHWRYKEKISKDDAAVDSFLKWARELIKDADTASAFMEGFKLNLYHDEIYVFT
PKGDMKILPAGATPIDFAYAIHTEIGNGCIGAKVNGKIVRLNAELRSGDRVEVITSKNQKPKPDWLKIVVTHRAKLKIRA
AINEERRKEIEKGRNIFDKMLTGTKRLFTENDAIRAIRKHGIKTPADLFSALANQQISSEEVMESITRPHGKGAEHEGDV
QGKAPHKEFAEIAREVQERPTSHKDEVTIAGMNNIAYSYAKCCNPVPGDDIIGFVTTEGAVKIHRKNCFNVTNENSVKSE
RIVSVAWNRKLNTEFLAGIRIVGEDKVGMTNQITGVISKFDTNIRTITLHAKDGIFVCNLMIFVKNTDKLTTLMDKLKKV
QGVFTVERMSA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=706, Percent_Identity=38.1019830028329, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1789147, Length=714, Percent_Identity=31.7927170868347, Blast_Score=406, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83356; Mature: 83356

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAQIEQEHYQKLHEILRLCRANLKNYDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHH
VAKLLVTELPLDGVSVAAALLHDVIEDSGYTYEDIAAELGTEVADIVEGLTKISGIMVNR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCEEEEC
ETTEAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLDSLPEHRRLRMALETRDIYAPLAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RFGLGKMKVDLENLAFKYIDPKMYDYLLKKVRLSRNERMAYLNKMIAPIKDDLEKQGFNV
HCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
EVQGRAKHLFSIYNKMRLKNKQFDDIHDLYGIRVIIDTDKLSDCFAVYGYITQKYPPIPQ
EECCHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCHH
HFKDYISIPKHNGYQSLHSAIIGPKGHVIELQIRTRRMHEFAELGVAAHWRYKEKISKDD
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCH
AAVDSFLKWARELIKDADTASAFMEGFKLNLYHDEIYVFTPKGDMKILPAGATPIDFAYA
HHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECEEEEECCCCCEEEEECCCCCCHHHEE
IHTEIGNGCIGAKVNGKIVRLNAELRSGDRVEVITSKNQKPKPDWLKIVVTHRAKLKIRA
EEHHCCCCEECCEECCEEEEEEECCCCCCEEEEEECCCCCCCCHHEEEEEECCCEEEEEE
AINEERRKEIEKGRNIFDKMLTGTKRLFTENDAIRAIRKHGIKTPADLFSALANQQISSE
HHCHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCHH
EVMESITRPHGKGAEHEGDVQGKAPHKEFAEIAREVQERPTSHKDEVTIAGMNNIAYSYA
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHH
KCCNPVPGDDIIGFVTTEGAVKIHRKNCFNVTNENSVKSERIVSVAWNRKLNTEFLAGIR
HHCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCHHHEEEEECCCCCCHHHHHCEE
IVGEDKVGMTNQITGVISKFDTNIRTITLHAKDGIFVCNLMIFVKNTDKLTTLMDKLKKV
EEECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEEEEECCHHHHHHHHHHHHH
QGVFTVERMSA
HHHHHHHHHCC
>Mature Secondary Structure
MLAQIEQEHYQKLHEILRLCRANLKNYDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHH
VAKLLVTELPLDGVSVAAALLHDVIEDSGYTYEDIAAELGTEVADIVEGLTKISGIMVNR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCEEEEC
ETTEAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLDSLPEHRRLRMALETRDIYAPLAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RFGLGKMKVDLENLAFKYIDPKMYDYLLKKVRLSRNERMAYLNKMIAPIKDDLEKQGFNV
HCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
EVQGRAKHLFSIYNKMRLKNKQFDDIHDLYGIRVIIDTDKLSDCFAVYGYITQKYPPIPQ
EECCHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCHH
HFKDYISIPKHNGYQSLHSAIIGPKGHVIELQIRTRRMHEFAELGVAAHWRYKEKISKDD
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCH
AAVDSFLKWARELIKDADTASAFMEGFKLNLYHDEIYVFTPKGDMKILPAGATPIDFAYA
HHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECEEEEECCCCCEEEEECCCCCCHHHEE
IHTEIGNGCIGAKVNGKIVRLNAELRSGDRVEVITSKNQKPKPDWLKIVVTHRAKLKIRA
EEHHCCCCEECCEECCEEEEEEECCCCCCEEEEEECCCCCCCCHHEEEEEECCCEEEEEE
AINEERRKEIEKGRNIFDKMLTGTKRLFTENDAIRAIRKHGIKTPADLFSALANQQISSE
HHCHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCHH
EVMESITRPHGKGAEHEGDVQGKAPHKEFAEIAREVQERPTSHKDEVTIAGMNNIAYSYA
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHH
KCCNPVPGDDIIGFVTTEGAVKIHRKNCFNVTNENSVKSERIVSVAWNRKLNTEFLAGIR
HHCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCHHHEEEEECCCCCCHHHHHCEE
IVGEDKVGMTNQITGVISKFDTNIRTITLHAKDGIFVCNLMIFVKNTDKLTTLMDKLKKV
EEECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEEEEECCHHHHHHHHHHHHH
QGVFTVERMSA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9383190; 9384377 [H]