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The map label for this gene is rpoA

Identifier: 193211878

GI number: 193211878

Start: 203403

End: 204389

Strand: Direct

Name: rpoA

Synonym: Cpar_0204

Alternate gene names: 193211878

Gene position: 203403-204389 (Clockwise)

Preceding gene: 193211877

Following gene: 193211879

Centisome position: 8.89

GC content: 52.99

Gene sequence:

>987_bases
ATGATATATCAGATGCAGATGCCTGCAAAGATCGATGTGGACGAAGCGACCCACACCGGCAGCTTCGGGCGATTCATAGC
CCAGCCGCTGGAGAGGGGATACGGAGTTACGCTGGGCAATGCCATGAGAAGGGTTCTGCTTGCCTCTCTTCCCGGAACAG
CAATCACCGGGATCAAGATCGATGGTGTGTTTCACGAGTTTTCAACGATCGACGGCGTTCGCGAGGATGTGCCCGAGATT
GTACTCAATCTGAAAAAAGTGCGCTTCAAGTCGAACTGCAAGCGCAGCTGCAAAACGACTCTGACCCTTGCGGGGCCTAA
AGATTTCCTTGCCGGCGACATCGTCGCACAGGAAGGTGAATTCGAGGTGCTCAACAAGGATCTGCATATCGCGACGATCA
ACTCTGAGGCTGCCGTGACGATCGATATTTATATCGGCCGCGGTCGTGGTTATGTGCCCGCCGAAGAGAATCGCAGCGAC
GGAATGCCGATCGGCTTCATCGCCATCGACTCGATTTATACACCGATCAAGAACGTCAAACTGACCGTCGAGAACACCCG
TGTCGGGCAGAAGACCGACTACGAAAAGATGATTCTCGATGTTGAGACCGACGGATCAATCTCTCCCGACGACGCCATCA
GCCTTGCCGGCAGGATCATCAACGATCACGTCACCTTCTTTGCGAACTTCTCGCCGACCGAAGAGGAGTATGCTGAAGAG
GAGTACAAGCAGCTCGACGAAGAGTTCGAGAACATGCGCAAAAAGCTCCAGACCAAGATCGAGGATCTCGACCTTTCGGT
TCGTTCGCACAACTGCCTGAGGCTTGCTGAAATCGACACGCTCGCTGATCTGGTTTCCAGAAGGGAAGATGAGCTGTTGA
ACTACAAGAACTTCGGCAAGAAGTCTTTGACAGAGTTGAGAGAGGCCCTTGAAAAAGAGGGGCTCAAGTTCGGGATGGAT
ATCACCCGTTACCAGATGAAAGGGTGA

Upstream 100 bases:

>100_bases
CTATTCCTGAGCGCGAAGCCGTACAGGAACCGTTCAATGAACAGCTTGTCGTCGAGTTGTACTCCAAGTGATCTTTTTGA
ATTCCTAAGGAAGCAAGACT

Downstream 100 bases:

>100_bases
ACGCAGGCCAATAGTACATTAGAAATCAATGAGAGCGGAAACAAGAAATGCGTAAAGTAAAGCCAGCAAGGAAACTCGGG
AGAACGACCGCCCATAGAAA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MIYQMQMPAKIDVDEATHTGSFGRFIAQPLERGYGVTLGNAMRRVLLASLPGTAITGIKIDGVFHEFSTIDGVREDVPEI
VLNLKKVRFKSNCKRSCKTTLTLAGPKDFLAGDIVAQEGEFEVLNKDLHIATINSEAAVTIDIYIGRGRGYVPAEENRSD
GMPIGFIAIDSIYTPIKNVKLTVENTRVGQKTDYEKMILDVETDGSISPDDAISLAGRIINDHVTFFANFSPTEEEYAEE
EYKQLDEEFENMRKKLQTKIEDLDLSVRSHNCLRLAEIDTLADLVSRREDELLNYKNFGKKSLTELREALEKEGLKFGMD
ITRYQMKG

Sequences:

>Translated_328_residues
MIYQMQMPAKIDVDEATHTGSFGRFIAQPLERGYGVTLGNAMRRVLLASLPGTAITGIKIDGVFHEFSTIDGVREDVPEI
VLNLKKVRFKSNCKRSCKTTLTLAGPKDFLAGDIVAQEGEFEVLNKDLHIATINSEAAVTIDIYIGRGRGYVPAEENRSD
GMPIGFIAIDSIYTPIKNVKLTVENTRVGQKTDYEKMILDVETDGSISPDDAISLAGRIINDHVTFFANFSPTEEEYAEE
EYKQLDEEFENMRKKLQTKIEDLDLSVRSHNCLRLAEIDTLADLVSRREDELLNYKNFGKKSLTELREALEKEGLKFGMD
ITRYQMKG
>Mature_328_residues
MIYQMQMPAKIDVDEATHTGSFGRFIAQPLERGYGVTLGNAMRRVLLASLPGTAITGIKIDGVFHEFSTIDGVREDVPEI
VLNLKKVRFKSNCKRSCKTTLTLAGPKDFLAGDIVAQEGEFEVLNKDLHIATINSEAAVTIDIYIGRGRGYVPAEENRSD
GMPIGFIAIDSIYTPIKNVKLTVENTRVGQKTDYEKMILDVETDGSISPDDAISLAGRIINDHVTFFANFSPTEEEYAEE
EYKQLDEEFENMRKKLQTKIEDLDLSVRSHNCLRLAEIDTLADLVSRREDELLNYKNFGKKSLTELREALEKEGLKFGMD
ITRYQMKG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=321, Percent_Identity=38.006230529595, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_CHLP8 (B3QR98)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001997831.1
- GeneID:   6419112
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0204
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 36860; Mature: 36860

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYQMQMPAKIDVDEATHTGSFGRFIAQPLERGYGVTLGNAMRRVLLASLPGTAITGIKI
CEEEECCCCEECCCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEEE
DGVFHEFSTIDGVREDVPEIVLNLKKVRFKSNCKRSCKTTLTLAGPKDFLAGDIVAQEGE
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHEECCCC
FEVLNKDLHIATINSEAAVTIDIYIGRGRGYVPAEENRSDGMPIGFIAIDSIYTPIKNVK
CEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCEE
LTVENTRVGQKTDYEKMILDVETDGSISPDDAISLAGRIINDHVTFFANFSPTEEEYAEE
EEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHH
EYKQLDEEFENMRKKLQTKIEDLDLSVRSHNCLRLAEIDTLADLVSRREDELLNYKNFGK
HHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCEEEHHHHHHHHHHHHHHHHHHCHHHCCH
KSLTELREALEKEGLKFGMDITRYQMKG
HHHHHHHHHHHHCCCEECCCEEEEEECC
>Mature Secondary Structure
MIYQMQMPAKIDVDEATHTGSFGRFIAQPLERGYGVTLGNAMRRVLLASLPGTAITGIKI
CEEEECCCCEECCCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEEE
DGVFHEFSTIDGVREDVPEIVLNLKKVRFKSNCKRSCKTTLTLAGPKDFLAGDIVAQEGE
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHEECCCC
FEVLNKDLHIATINSEAAVTIDIYIGRGRGYVPAEENRSDGMPIGFIAIDSIYTPIKNVK
CEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCEE
LTVENTRVGQKTDYEKMILDVETDGSISPDDAISLAGRIINDHVTFFANFSPTEEEYAEE
EEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHH
EYKQLDEEFENMRKKLQTKIEDLDLSVRSHNCLRLAEIDTLADLVSRREDELLNYKNFGK
HHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCEEEHHHHHHHHHHHHHHHHHHCHHHCCH
KSLTELREALEKEGLKFGMDITRYQMKG
HHHHHHHHHHHHCCCEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA