Click here to switch to the map view.

The map label for this gene is tuf

Identifier: 193211849

GI number: 193211849

Start: 188146

End: 189327

Strand: Direct

Name: tuf

Synonym: Cpar_0175

Alternate gene names: 193211849

Gene position: 188146-189327 (Clockwise)

Preceding gene: 193211848

Following gene: 193211850

Centisome position: 8.22

GC content: 55.92

Gene sequence:

>1182_bases
ATGGCTAAAGAGTCATACAAGAGGGATAAACCTCACGTAAATATTGGTACTATCGGTCACGTTGACCACGGCAAAACCAC
CCTTACCGCAGCAATCACCTCGGTGCTTGCTAAATCTGGTAAAGCAGCAGCTCGTGAGTTTGGTGACATCGATAAGGCTC
CGGAAGAGAGGGAGCGCGGTATTACCATCTCGACCGCACACGTCGAGTACCAGACCGACAAGCGTCACTATGCACACATC
GACTGCCCCGGTCACGCTGACTACATTAAAAACATGATCACCGGTGCTGCCCAGATGGACGGCGCTATCCTCGTCGTTGC
CGGTACCGACGGCCCGATGCCTCAGACTCGTGAGCACATCCTGCTCGCCCGCCAGGTGAACGTTCCTGCGCTGGTCGTCT
TCCTGAACAAGGTTGACATCGCCGACCCGGAACTTCTCGAGCTGGTCGAAATGGAACTCCGTGAGCTTCTCACCGAGTAC
GGTTTCCCGGGCGACGATATTCCGATCATCAAGGGTTCCGCTCTGAACGCCCTGAACGGCGATCCGGAAGGCGAGAAAGC
CATCATGGAGCTCATGGACGCTGTTGACGACTACATTCCTGAGCCGGTTCGTGACGTTGACAAGCCGTTCCTGATGCCGG
TCGAAGACGTGTTCTCGATCTCTGGTCGTGGTACTGTCGGTACCGGTCGTATCGAGAGGGGCATCATCAAGGTTGGTAAC
GAAGTCGAAATCGTCGGTATCAAGCCGACCACCAAATCGGTTGTTACCGGTATCGAAATGTTCCAGAAGACCCTCGACGA
AGGTCAGGCAGGTGACAACGCCGGCTTGCTCCTCCGCGGTGTGGACAAAGAGGCGCTCGAGCGTGGTATGGTTATCGCCA
AGCCTGGTTCGATCACCCCGCACACCAAGTTCAAGGCTGAGGTGTACATCCTGAAGAAGGAAGAGGGCGGTCGTCATACT
CCGTTCTTCAACGGCTATCGTCCGCAGTTCTATTTCAGAACCACTGACGTGACCGGTTCGGTAACTCTCCCTGAGGGTGT
CGAGATGGTCATGCCTGGTGACAACCTCTCCGTCGATGTCGAGCTGATCGCTCCTATCGCTATGGAAGAGAGCCTCAGGT
TCGCTATCCGCGAGGGCGGCCGTACGGTTGGTGCCGGTTCGGTAACCAAGATCGTCGAGTAA

Upstream 100 bases:

>100_bases
TATTGCTGAGACCTTGCAGGAGAAAAGGGTCGGAAAGGATTCCGAATAACAGTTTTTTTCACCGCATCACTAAACAAGAT
AACCGACAGGGAGAACAGCT

Downstream 100 bases:

>100_bases
TCGACAGATTGTTATCCCGGGGCGAGGCAGCGCTCTCGCCCCTTTTTTATTGAAACCATCGAAACAGGGATAGACCAGTG
GCTGTTCAGCAAAAGATCAG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKSGKAAAREFGDIDKAPEERERGITISTAHVEYQTDKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVFLNKVDIADPELLELVEMELRELLTEY
GFPGDDIPIIKGSALNALNGDPEGEKAIMELMDAVDDYIPEPVRDVDKPFLMPVEDVFSISGRGTVGTGRIERGIIKVGN
EVEIVGIKPTTKSVVTGIEMFQKTLDEGQAGDNAGLLLRGVDKEALERGMVIAKPGSITPHTKFKAEVYILKKEEGGRHT
PFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSVDVELIAPIAMEESLRFAIREGGRTVGAGSVTKIVE

Sequences:

>Translated_393_residues
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKSGKAAAREFGDIDKAPEERERGITISTAHVEYQTDKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVFLNKVDIADPELLELVEMELRELLTEY
GFPGDDIPIIKGSALNALNGDPEGEKAIMELMDAVDDYIPEPVRDVDKPFLMPVEDVFSISGRGTVGTGRIERGIIKVGN
EVEIVGIKPTTKSVVTGIEMFQKTLDEGQAGDNAGLLLRGVDKEALERGMVIAKPGSITPHTKFKAEVYILKKEEGGRHT
PFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSVDVELIAPIAMEESLRFAIREGGRTVGAGSVTKIVE
>Mature_392_residues
AKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKSGKAAAREFGDIDKAPEERERGITISTAHVEYQTDKRHYAHID
CPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVFLNKVDIADPELLELVEMELRELLTEYG
FPGDDIPIIKGSALNALNGDPEGEKAIMELMDAVDDYIPEPVRDVDKPFLMPVEDVFSISGRGTVGTGRIERGIIKVGNE
VEIVGIKPTTKSVVTGIEMFQKTLDEGQAGDNAGLLLRGVDKEALERGMVIAKPGSITPHTKFKAEVYILKKEEGGRHTP
FFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSVDVELIAPIAMEESLRFAIREGGRTVGAGSVTKIVE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.6294416243655, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=31.651376146789, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=31.651376146789, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=30.0448430493274, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=29.8206278026906, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=32.0809248554913, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI194018522, Length=436, Percent_Identity=26.605504587156, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194018520, Length=436, Percent_Identity=26.605504587156, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194097354, Length=436, Percent_Identity=26.605504587156, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI46094014, Length=443, Percent_Identity=26.86230248307, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4503507, Length=264, Percent_Identity=31.4393939393939, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI157426893, Length=235, Percent_Identity=27.6595744680851, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI94966754, Length=131, Percent_Identity=35.1145038167939, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI53729339, Length=235, Percent_Identity=30.6382978723404, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI53729337, Length=235, Percent_Identity=30.6382978723404, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=72.0101781170484, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=72.0101781170484, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=381, Percent_Identity=30.7086614173228, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI48994988, Length=253, Percent_Identity=33.201581027668, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789108, Length=326, Percent_Identity=29.4478527607362, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1788922, Length=140, Percent_Identity=36.4285714285714, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.8847117794486, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25141371, Length=370, Percent_Identity=45.945945945946, Blast_Score=340, Evalue=9e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=31.4855875831486, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=31.4855875831486, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=428, Percent_Identity=31.3084112149533, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI115532067, Length=446, Percent_Identity=28.6995515695067, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI115532065, Length=446, Percent_Identity=28.6995515695067, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=439, Percent_Identity=26.879271070615, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=297, Percent_Identity=29.6296296296296, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=150, Percent_Identity=42, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=281, Percent_Identity=29.5373665480427, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=230, Percent_Identity=29.5652173913043, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71994658, Length=198, Percent_Identity=35.3535353535354, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=67.5062972292191, Blast_Score=531, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=30, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=30, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=460, Percent_Identity=24.5652173913043, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=276, Percent_Identity=30.7971014492754, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320377, Length=344, Percent_Identity=28.1976744186047, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=30.672268907563, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=140, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=58.6118251928021, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=58.6118251928021, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=48.0519480519481, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=30.6487695749441, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=30.6487695749441, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=30.6487695749441, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=30.6487695749441, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=30.4932735426009, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=30.4932735426009, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=33.5526315789474, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=27.2930648769575, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17137380, Length=448, Percent_Identity=25.6696428571429, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=28.9285714285714, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=28.9285714285714, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28572034, Length=168, Percent_Identity=35.1190476190476, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78706572, Length=244, Percent_Identity=26.6393442622951, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582462, Length=131, Percent_Identity=34.3511450381679, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_CHLP8 (P42473)

Other databases:

- EMBL:   X77033
- EMBL:   CP001099
- RefSeq:   YP_001997802.1
- ProteinModelPortal:   P42473
- SMR:   P42473
- GeneID:   6419082
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0175
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 42699; Mature: 42568

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKSGKAAAREFGDIDKAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHCC
ITISTAHVEYQTDKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVNVPALVVFLNKVDIADPELLELVEMELRELLTEYGFPGDDIPIIKGSALNALNG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
DPEGEKAIMELMDAVDDYIPEPVRDVDKPFLMPVEDVFSISGRGTVGTGRIERGIIKVGN
CCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCHHHCCCEECCC
EVEIVGIKPTTKSVVTGIEMFQKTLDEGQAGDNAGLLLRGVDKEALERGMVIAKPGSITP
EEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCEEEECCCCCCC
HTKFKAEVYILKKEEGGRHTPFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSVDV
CCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCCEEEE
ELIAPIAMEESLRFAIREGGRTVGAGSVTKIVE
EEECCHHHHHHHHHHHHCCCCEECCCCHHHHCC
>Mature Secondary Structure 
AKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKSGKAAAREFGDIDKAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHCC
ITISTAHVEYQTDKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVNVPALVVFLNKVDIADPELLELVEMELRELLTEYGFPGDDIPIIKGSALNALNG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
DPEGEKAIMELMDAVDDYIPEPVRDVDKPFLMPVEDVFSISGRGTVGTGRIERGIIKVGN
CCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCHHHCCCEECCC
EVEIVGIKPTTKSVVTGIEMFQKTLDEGQAGDNAGLLLRGVDKEALERGMVIAKPGSITP
EEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCEEEECCCCCCC
HTKFKAEVYILKKEEGGRHTPFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSVDV
CCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCCEEEE
ELIAPIAMEESLRFAIREGGRTVGAGSVTKIVE
EEECCHHHHHHHHHHHHCCCCEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8085791