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The map label for this gene is gatB

Identifier: 193211827

GI number: 193211827

Start: 166187

End: 167614

Strand: Direct

Name: gatB

Synonym: Cpar_0153

Alternate gene names: 193211827

Gene position: 166187-167614 (Clockwise)

Preceding gene: 193211826

Following gene: 193211829

Centisome position: 7.26

GC content: 60.15

Gene sequence:

>1428_bases
ATGAACTATGAAATTGTCGTGGGCCTTGAGGTCCACTGTCAGCTCAATACCGAAAGCAAGGCCTTTTGCGGCTGTTCAGC
AACCTTCGGCAAGGCGGCCAACACCAACGTCTGCCCTGTCTGCCTTGCGTTGCCCGGCGCGCTGCCGGTGCTGAACGCCC
GTGTGGTGGAGGACGCCGTGAAGCTCGGCCTCGCCACCAACTGCACCATTGCACGCCACTCCATCATGGCGCGCAAGAAC
TACTTCTATCCCGACCTTCCGAAGGGCTACCAGATTTCGCAGTTCGAGGAGCCGATCTGCTCGGAGGGTATGATTCACGT
CGAACTCGAAGAGGGGCGCAAGGATGTTCGTCTCGTCCGAATTCACATCGAAGAGGATGCGGGCAAGTCGATTCACGACA
TCGGTGACGACACCTACATCGACGTGAACCGTTGCGGCGTGCCGCTGCTGGAGATCGTGAGCTACCCCGACATGCGCACG
CCTAAAGAGGCCTCGGCCTATCTGCAGAAGCTGCGCCAGATCGTCAAGTACCTCGGCATCTCCGATGGCAACATGGAGGA
GGGCAGCCTTCGTTGCGACGCCAACGTGTCGGTCAGGCCGGTGGGCGCGACCGAGTACGGCACCCGCACCGAGATCAAGA
ACATGAACTCGTTCCGCAACGTCGAGCGCGCCATCGAGTACGAGGCCAAGCGCCACATCGAGGTGATCGAGGGCGGTGGC
GTGATCGTTCAGGAGACGCGCCTGTGGGACGCTGACAAGCTCGAAACCCGCTCGATGCGCGGCAAGGAGCATGCCCACGA
CTACCGCTATTTCCCCGATCCGGATCTCGTGCCGGTGCTGGTCGATGACGGCATGATGCACAGGATGCGCGAAGAGCTGC
CGGAGTTTCCGGAAGATCGCGCGGCGCGTTTCGTTTCGGAGTTCGGCATTCCTGCGTATGACGCTGGCGTCATCACCGTG
GAGCGCGAGCTGGCCGACTATTTCGAGGCGACCGTGAACGTTTCAGGCGACGCCAAAGCCTCGTCGAACTGGGTGATGGG
CGAGGTGATGCGCACGCTGAAAGAGAAGTACCTCGATATTCATGAATTCGCCATTTTGCCGGAGCGGCTCGGCGGCCTGA
TCAAGCTCATCGGTGCGGGCGCGATCAGCAATACCATCGCCAAGCAGGTGTTCGAGATCATGCAGCAGGACGAAGCGACG
GCGGAGGAGATCGTCAAGCGCGAAGGGCTGGCGCAGGTCTCCGATACGGGTGCGATCGAGGCGGCGATCAAGGAGATTCT
CGACGCAAACCAGAAGCAGCTCGAACAGTACCGCAGCGGCAAGACGCAGCTGTTCGGCTTTTTTGTCGGTCAGTGCATGC
AGAAGATGAAGGGGAAGGCCAACCCGAAGGTGGTCAACGAAATTCTCAAAAAGCTGCTGGACGCCTGA

Upstream 100 bases:

>100_bases
GTGAGGCGACCATTGGCGGCCAGAAGTTCAGCTTCAAGCCGGGACGGTAAGCTCCAGCTGGTTTCGATAAGGTTTTTTCA
CTTTTTCAGTTTCTGCTTCA

Downstream 100 bases:

>100_bases
GAATTCTCAGCTCTCCATGTCGAACAGGCCGGCCGCTTTCGGTGGCTCCGGTTTGGCAGGCTTCTCGGACTGAGCAGGCA
GTGTGGCCTGAGCGGGCTTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNYEIVVGLEVHCQLNTESKAFCGCSATFGKAANTNVCPVCLALPGALPVLNARVVEDAVKLGLATNCTIARHSIMARKN
YFYPDLPKGYQISQFEEPICSEGMIHVELEEGRKDVRLVRIHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDMRT
PKEASAYLQKLRQIVKYLGISDGNMEEGSLRCDANVSVRPVGATEYGTRTEIKNMNSFRNVERAIEYEAKRHIEVIEGGG
VIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDDGMMHRMREELPEFPEDRAARFVSEFGIPAYDAGVITV
ERELADYFEATVNVSGDAKASSNWVMGEVMRTLKEKYLDIHEFAILPERLGGLIKLIGAGAISNTIAKQVFEIMQQDEAT
AEEIVKREGLAQVSDTGAIEAAIKEILDANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKVVNEILKKLLDA

Sequences:

>Translated_475_residues
MNYEIVVGLEVHCQLNTESKAFCGCSATFGKAANTNVCPVCLALPGALPVLNARVVEDAVKLGLATNCTIARHSIMARKN
YFYPDLPKGYQISQFEEPICSEGMIHVELEEGRKDVRLVRIHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDMRT
PKEASAYLQKLRQIVKYLGISDGNMEEGSLRCDANVSVRPVGATEYGTRTEIKNMNSFRNVERAIEYEAKRHIEVIEGGG
VIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDDGMMHRMREELPEFPEDRAARFVSEFGIPAYDAGVITV
ERELADYFEATVNVSGDAKASSNWVMGEVMRTLKEKYLDIHEFAILPERLGGLIKLIGAGAISNTIAKQVFEIMQQDEAT
AEEIVKREGLAQVSDTGAIEAAIKEILDANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKVVNEILKKLLDA
>Mature_475_residues
MNYEIVVGLEVHCQLNTESKAFCGCSATFGKAANTNVCPVCLALPGALPVLNARVVEDAVKLGLATNCTIARHSIMARKN
YFYPDLPKGYQISQFEEPICSEGMIHVELEEGRKDVRLVRIHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDMRT
PKEASAYLQKLRQIVKYLGISDGNMEEGSLRCDANVSVRPVGATEYGTRTEIKNMNSFRNVERAIEYEAKRHIEVIEGGG
VIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDDGMMHRMREELPEFPEDRAARFVSEFGIPAYDAGVITV
ERELADYFEATVNVSGDAKASSNWVMGEVMRTLKEKYLDIHEFAILPERLGGLIKLIGAGAISNTIAKQVFEIMQQDEAT
AEEIVKREGLAQVSDTGAIEAAIKEILDANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKVVNEILKKLLDA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=36.1616161616162, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI115534094, Length=302, Percent_Identity=38.4105960264901, Blast_Score=197, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=27.8846153846154, Blast_Score=202, Evalue=1e-52,
Organism=Drosophila melanogaster, GI45550808, Length=490, Percent_Identity=34.4897959183673, Blast_Score=289, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_CHLP8 (B3QRD6)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001997780.1
- GeneID:   6419060
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0153
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53060; Mature: 53060

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYEIVVGLEVHCQLNTESKAFCGCSATFGKAANTNVCPVCLALPGALPVLNARVVEDAV
CCEEEEEEEEEEEEECCCCCCEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
KLGLATNCTIARHSIMARKNYFYPDLPKGYQISQFEEPICSEGMIHVELEEGRKDVRLVR
HHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCHHCCCEEEEEECCCCCCEEEEE
IHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDMRTPKEASAYLQKLRQIVKYLGI
EEEECCCCCHHHHCCCCCEEEHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
SDGNMEEGSLRCDANVSVRPVGATEYGTRTEIKNMNSFRNVERAIEYEAKRHIEVIEGGG
CCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
VIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDDGMMHRMREELPEFPEDR
EEEEECCCCCCHHHHHHHHCCCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHH
AARFVSEFGIPAYDAGVITVERELADYFEATVNVSGDAKASSNWVMGEVMRTLKEKYLDI
HHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HEFAILPERLGGLIKLIGAGAISNTIAKQVFEIMQQDEATAEEIVKREGLAQVSDTGAIE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCHHHH
AAIKEILDANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKVVNEILKKLLDA
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYEIVVGLEVHCQLNTESKAFCGCSATFGKAANTNVCPVCLALPGALPVLNARVVEDAV
CCEEEEEEEEEEEEECCCCCCEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
KLGLATNCTIARHSIMARKNYFYPDLPKGYQISQFEEPICSEGMIHVELEEGRKDVRLVR
HHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCHHCCCEEEEEECCCCCCEEEEE
IHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDMRTPKEASAYLQKLRQIVKYLGI
EEEECCCCCHHHHCCCCCEEEHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
SDGNMEEGSLRCDANVSVRPVGATEYGTRTEIKNMNSFRNVERAIEYEAKRHIEVIEGGG
CCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
VIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDDGMMHRMREELPEFPEDR
EEEEECCCCCCHHHHHHHHCCCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHH
AARFVSEFGIPAYDAGVITVERELADYFEATVNVSGDAKASSNWVMGEVMRTLKEKYLDI
HHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HEFAILPERLGGLIKLIGAGAISNTIAKQVFEIMQQDEATAEEIVKREGLAQVSDTGAIE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCHHHH
AAIKEILDANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKVVNEILKKLLDA
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA