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The map label for this gene is exoA [H]

Identifier: 193211783

GI number: 193211783

Start: 128756

End: 129517

Strand: Direct

Name: exoA [H]

Synonym: Cpar_0108

Alternate gene names: 193211783

Gene position: 128756-129517 (Clockwise)

Preceding gene: 193211782

Following gene: 193211784

Centisome position: 5.62

GC content: 61.02

Gene sequence:

>762_bases
ATGAATATCTATACCTGGAATATCAATGGTATCCGCGCCCGGCAGGAGGCGCTCGCTGCGTGGCTCGACAGCCGCAAGCC
GGACTTCGTCGTGCTCGAAGAGGTGAAGGCGCGCGAGGAGGAGATTCCCGAAACGATCACAGATTTTGCCGGTTACAGGA
AATTCTGGAACGGCTCGTCGTTCAAAAAAGGGTACAGCGGCGTCGGCCTGCTGGTCCGCGAGGAGAGTCTCGGCGATGTC
AGTTGCGAAGTACCGCCGTTCGATGTGGAGAACCGCACGCTCGTGCTGCACGCTCCGCAATTCACCCTGATCGGCACCTA
TGTTCCTCGTGGCAACGGCGCGGAGCACTACGCGGTGAAGCTTCGCTATCTTGCGGATCTGAAGGAATATATCGCCAGCC
TCATTGCTGAGGGGCAGGAGGTCATTCTGGCCGGCGACATGAACGTGGCGCTCACGGAGATCGACGTGCATCGTTCGCAA
AACAAGCCCGGCGCAACCGGCCTGCGCCCCGAGGAGCGCTCGGCCATCGAGGCGCATCTCGAACTCGGCCTGCACGACAT
TGTCCGCGAGCTGAATCCCGACAAGAAGGACCTGTTCACCTGGTGGCCCAACTGGAAGTTCGCCCGCGAGCGCAACCTCG
GCTGGCGCATCGACTGCATCTACCTCTCCCCCGCGCTTGCCGGCAAGGTTTCCGGCGTTTCGGTCGATCTCGACGAGAAG
AGTTCCGACCACGCTCCGGTGTCGGTGGAGCTTGCGTTCTGA

Upstream 100 bases:

>100_bases
GTCGAAGCAAATGCCCGAAACGGCACTTCCGCTGCATGATGGAGATCGCGCCAGAGCCCATTGCGGCACGCATCGTCGAA
ACGCTCAACAAGAACTCAGC

Downstream 100 bases:

>100_bases
TCGGTTTTCAGCCCTTCCATCCGGCAGCCATCGCTCGTCAGTTGCGCGTTGGAAAACTGAATTAATATTCTTACTTTGGA
TGTCCTAAAACGCAAACGGT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNIYTWNINGIRARQEALAAWLDSRKPDFVVLEEVKAREEEIPETITDFAGYRKFWNGSSFKKGYSGVGLLVREESLGDV
SCEVPPFDVENRTLVLHAPQFTLIGTYVPRGNGAEHYAVKLRYLADLKEYIASLIAEGQEVILAGDMNVALTEIDVHRSQ
NKPGATGLRPEERSAIEAHLELGLHDIVRELNPDKKDLFTWWPNWKFARERNLGWRIDCIYLSPALAGKVSGVSVDLDEK
SSDHAPVSVELAF

Sequences:

>Translated_253_residues
MNIYTWNINGIRARQEALAAWLDSRKPDFVVLEEVKAREEEIPETITDFAGYRKFWNGSSFKKGYSGVGLLVREESLGDV
SCEVPPFDVENRTLVLHAPQFTLIGTYVPRGNGAEHYAVKLRYLADLKEYIASLIAEGQEVILAGDMNVALTEIDVHRSQ
NKPGATGLRPEERSAIEAHLELGLHDIVRELNPDKKDLFTWWPNWKFARERNLGWRIDCIYLSPALAGKVSGVSVDLDEK
SSDHAPVSVELAF
>Mature_253_residues
MNIYTWNINGIRARQEALAAWLDSRKPDFVVLEEVKAREEEIPETITDFAGYRKFWNGSSFKKGYSGVGLLVREESLGDV
SCEVPPFDVENRTLVLHAPQFTLIGTYVPRGNGAEHYAVKLRYLADLKEYIASLIAEGQEVILAGDMNVALTEIDVHRSQ
NKPGATGLRPEERSAIEAHLELGLHDIVRELNPDKKDLFTWWPNWKFARERNLGWRIDCIYLSPALAGKVSGVSVDLDEK
SSDHAPVSVELAF

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=260, Percent_Identity=31.5384615384615, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI18375503, Length=260, Percent_Identity=31.5384615384615, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI18375501, Length=260, Percent_Identity=31.5384615384615, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=29.6992481203008, Blast_Score=117, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71989536, Length=265, Percent_Identity=32.4528301886792, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI221330655, Length=258, Percent_Identity=30.2325581395349, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17136678, Length=258, Percent_Identity=30.2325581395349, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28482; Mature: 28482

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYTWNINGIRARQEALAAWLDSRKPDFVVLEEVKAREEEIPETITDFAGYRKFWNGSS
CEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FKKGYSGVGLLVREESLGDVSCEVPPFDVENRTLVLHAPQFTLIGTYVPRGNGAEHYAVK
CCCCCCCEEEEEEECCCCCEECCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEEE
LRYLADLKEYIASLIAEGQEVILAGDMNVALTEIDVHRSQNKPGATGLRPEERSAIEAHL
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHH
ELGLHDIVRELNPDKKDLFTWWPNWKFARERNLGWRIDCIYLSPALAGKVSGVSVDLDEK
HCCHHHHHHHCCCCHHHEEEECCCCEEECCCCCCEEEEEEEECCHHHCCCCCEEEECCCC
SSDHAPVSVELAF
CCCCCCEEEEEEC
>Mature Secondary Structure
MNIYTWNINGIRARQEALAAWLDSRKPDFVVLEEVKAREEEIPETITDFAGYRKFWNGSS
CEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FKKGYSGVGLLVREESLGDVSCEVPPFDVENRTLVLHAPQFTLIGTYVPRGNGAEHYAVK
CCCCCCCEEEEEEECCCCCEECCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEEE
LRYLADLKEYIASLIAEGQEVILAGDMNVALTEIDVHRSQNKPGATGLRPEERSAIEAHL
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHH
ELGLHDIVRELNPDKKDLFTWWPNWKFARERNLGWRIDCIYLSPALAGKVSGVSVDLDEK
HCCHHHHHHHCCCCHHHEEEECCCCEEECCCCCCEEEEEEEECCHHHCCCCCEEEECCCC
SSDHAPVSVELAF
CCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]