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The map label for this gene is fhlA [H]

Identifier: 193211733

GI number: 193211733

Start: 65447

End: 67552

Strand: Direct

Name: fhlA [H]

Synonym: Cpar_0058

Alternate gene names: 193211733

Gene position: 65447-67552 (Clockwise)

Preceding gene: 193211728

Following gene: 193211734

Centisome position: 2.86

GC content: 63.2

Gene sequence:

>2106_bases
ATGACAACCACGCACTTGCCCGAGGCGCTTCGGCTCATGCAGTATATCGGCGACGCCATCGGCACCATCCGTGACCCGCA
GGAGCTGTTCCGCACGGTCACCGACAAGCTTCGCCTCATCTTTCCGTTCGATTCGGCGGTGATCATCACCCTCGACCGGG
ACCGCCGCCGCGTGAGTGTTTTTTTCGAGATGCTGCGCTTCGAGTTGCCCGACCATCTGCGGGTGCAGCGGCGCCCCATT
ACCGGCACCTGGCTTGAGGCGCATCTCGACGACCGCGGGGTGACCGTCGCCTCCATCGCTCACGATATTCCTGCGTTTGG
CGAGGAGGATGCTCCGCTGCTCCGGACGCTGCACGAGTTGGGAATGCGGCAGATTGTGCTTTCGCCGCTTCGTTCCGGTG
GGCGGGTGATCGGTTTTCTCAGCTTTGTTTCTGCAGAGGAGAAGCTGTGGAGCGATGGCGATAAATCGCTGCTTTCCGGG
GTCAGCTCCTCCATCGCCATCGCGGTGAGCAATGCGCTGGCCTACGACGAGCTGCGCCAGCGCGAGGCGGAAACCGCCAT
GCAGCTTGCGATCAACAATGCACTCTTCACCACCAAGGATCGAAGCCAGATGCTGCTGACTGTCTGCGAGCAGATCAGCC
GCCTCGTGCCCTGCTCGTTTCTTGGCATCCGCGTGGTTGGTTCGGACGGGCGGTTCCGTATTTACGATAACTTCATGCGC
GGGTCGGGCAGCAGTTTCGCGCCCTTTTCGCCACTCGAACATCTTGAAATGTTGCCCGACGACCCCGTTGCGCGGGAGAG
CATTGAGGTGATTTCGCGGCCCGGCATCTATTCCGGCGAGCGCTTTGAAGAGCTGTGCCGGGATTTTCGCATTCTCGAAC
TGGTGCGCGATCGCTTCGGCATCAGCTCGATCATCGTCGTGCAGCTTTGGGATTTGCCCGGCAGCCGCGCGGGGCTGATC
ATTTCAGGCGTCGGCGTTACGCTGGGCGACGAGGAGGCGCGCACGGTCAGCCTCATTGTGCCACAGCTCGCGCTGGCATT
GCAGAACTACCTCGCTTTCGACGAGATCGACCGGCTGCGCCGCAAGCTCGAAGGGGAGCGCACCTACCTCGTCGAGGAGA
TCCGCGCTGCACACAACTTCGAAGAGATCGTCGGCAACAGCGCACCGCTGGCCGAGGTGCTGCGGCGAGTCAGCCTGGTT
GCTCCGACCGATGCCACGGTGCTTATCGAGGGCGAAACCGGCACCGGCAAGGAGCTGATCGCCCGCGCGATCCACAACCT
TTCGCCGCGCAAGGAGCGGGTGCTGGTGAAGGTCAACTGCGCCGCCCTGCCCGCATCGCTGATCGAGTCCGAGCTGTTCG
GCCACGAAAAGGGGAGCTTCACCGGCGCGACTGAACGGCGCATCGGCAAGTTCGAGCTGGCCGACGGCGGCACGATCTTC
CTCGACGAAATAGGCGAGCTTCCGCTGGAGCTTCAGGCCAAGCTGCTGCGGGTGCTTCAGGAGAAGGAGCTCGAACGCAT
CGGCGGGCGGAGCGTGATTCCGGTCGATGTGCGGGTCATCGCCGCCACCAACCGCGACCTTGAAAAGGAGGTCGCCACCG
GTCGCTTCCGTCAGGATCTCTACTTCCGGCTCAACGCCTTTCCCCTATCGGTGCCGCCGCTTCGCGAACGCCGCGACGAC
ATCCCCGTGCTCGCCATGTACTTTGCCCGCAAATTCGCTCGCGAGTTCGGCAAGCCGGAACGCGCCATCCGTGAGCACGA
TATGAACGAGCTGGTCTCGCGAGAGTGGCGGGGCAACATCCGTGAACTCTCCCACACCATTGAGCAGGCGGTGATTGTCT
CCGAAGGTGACACGCTCGACCTCTCCTCAGCCCTGCCTCCGAGGGGTGAGCCTGGCATGGAGGCGCAGGCGGAGGTCATG
ACGATGCAGGAGTTCGAGGAGCAGGCGCGGAACATGGAGCGCAAGCTCATTCTCGATGCCCTCGACCGCGCCGGAGGCAA
GGTCAGCGGTCAGGGTGGCGCGGCCGAGCTGCTCAGGCTCAACGCCAAGACGCTCTATTCCCGCATCGACAAGCTGGGAA
TTCGCAAGCGGTACGGGGCGGGGTGA

Upstream 100 bases:

>100_bases
CCTGCTTTTCAAGGCTTTGAGCTTGTTATTTATTTCTTGTTTTTCTGAAAATTTCAGAAAATTCTCTTAACTTCCTTCTG
AATAAATCAGAAAATCAGCT

Downstream 100 bases:

>100_bases
GCAAGGATTTGCTTACATTAACCCATATGCTTCTTTTTTTCAGTCTTATCGTTGCTTCCGGTTCTTTTGAAACAACGCCA
ACCCCTAAATCAAAAAGATC

Product: NifA subfamily transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MTTTHLPEALRLMQYIGDAIGTIRDPQELFRTVTDKLRLIFPFDSAVIITLDRDRRRVSVFFEMLRFELPDHLRVQRRPI
TGTWLEAHLDDRGVTVASIAHDIPAFGEEDAPLLRTLHELGMRQIVLSPLRSGGRVIGFLSFVSAEEKLWSDGDKSLLSG
VSSSIAIAVSNALAYDELRQREAETAMQLAINNALFTTKDRSQMLLTVCEQISRLVPCSFLGIRVVGSDGRFRIYDNFMR
GSGSSFAPFSPLEHLEMLPDDPVARESIEVISRPGIYSGERFEELCRDFRILELVRDRFGISSIIVVQLWDLPGSRAGLI
ISGVGVTLGDEEARTVSLIVPQLALALQNYLAFDEIDRLRRKLEGERTYLVEEIRAAHNFEEIVGNSAPLAEVLRRVSLV
APTDATVLIEGETGTGKELIARAIHNLSPRKERVLVKVNCAALPASLIESELFGHEKGSFTGATERRIGKFELADGGTIF
LDEIGELPLELQAKLLRVLQEKELERIGGRSVIPVDVRVIAATNRDLEKEVATGRFRQDLYFRLNAFPLSVPPLRERRDD
IPVLAMYFARKFAREFGKPERAIREHDMNELVSREWRGNIRELSHTIEQAVIVSEGDTLDLSSALPPRGEPGMEAQAEVM
TMQEFEEQARNMERKLILDALDRAGGKVSGQGGAAELLRLNAKTLYSRIDKLGIRKRYGAG

Sequences:

>Translated_701_residues
MTTTHLPEALRLMQYIGDAIGTIRDPQELFRTVTDKLRLIFPFDSAVIITLDRDRRRVSVFFEMLRFELPDHLRVQRRPI
TGTWLEAHLDDRGVTVASIAHDIPAFGEEDAPLLRTLHELGMRQIVLSPLRSGGRVIGFLSFVSAEEKLWSDGDKSLLSG
VSSSIAIAVSNALAYDELRQREAETAMQLAINNALFTTKDRSQMLLTVCEQISRLVPCSFLGIRVVGSDGRFRIYDNFMR
GSGSSFAPFSPLEHLEMLPDDPVARESIEVISRPGIYSGERFEELCRDFRILELVRDRFGISSIIVVQLWDLPGSRAGLI
ISGVGVTLGDEEARTVSLIVPQLALALQNYLAFDEIDRLRRKLEGERTYLVEEIRAAHNFEEIVGNSAPLAEVLRRVSLV
APTDATVLIEGETGTGKELIARAIHNLSPRKERVLVKVNCAALPASLIESELFGHEKGSFTGATERRIGKFELADGGTIF
LDEIGELPLELQAKLLRVLQEKELERIGGRSVIPVDVRVIAATNRDLEKEVATGRFRQDLYFRLNAFPLSVPPLRERRDD
IPVLAMYFARKFAREFGKPERAIREHDMNELVSREWRGNIRELSHTIEQAVIVSEGDTLDLSSALPPRGEPGMEAQAEVM
TMQEFEEQARNMERKLILDALDRAGGKVSGQGGAAELLRLNAKTLYSRIDKLGIRKRYGAG
>Mature_700_residues
TTTHLPEALRLMQYIGDAIGTIRDPQELFRTVTDKLRLIFPFDSAVIITLDRDRRRVSVFFEMLRFELPDHLRVQRRPIT
GTWLEAHLDDRGVTVASIAHDIPAFGEEDAPLLRTLHELGMRQIVLSPLRSGGRVIGFLSFVSAEEKLWSDGDKSLLSGV
SSSIAIAVSNALAYDELRQREAETAMQLAINNALFTTKDRSQMLLTVCEQISRLVPCSFLGIRVVGSDGRFRIYDNFMRG
SGSSFAPFSPLEHLEMLPDDPVARESIEVISRPGIYSGERFEELCRDFRILELVRDRFGISSIIVVQLWDLPGSRAGLII
SGVGVTLGDEEARTVSLIVPQLALALQNYLAFDEIDRLRRKLEGERTYLVEEIRAAHNFEEIVGNSAPLAEVLRRVSLVA
PTDATVLIEGETGTGKELIARAIHNLSPRKERVLVKVNCAALPASLIESELFGHEKGSFTGATERRIGKFELADGGTIFL
DEIGELPLELQAKLLRVLQEKELERIGGRSVIPVDVRVIAATNRDLEKEVATGRFRQDLYFRLNAFPLSVPPLRERRDDI
PVLAMYFARKFAREFGKPERAIREHDMNELVSREWRGNIRELSHTIEQAVIVSEGDTLDLSSALPPRGEPGMEAQAEVMT
MQEFEEQARNMERKLILDALDRAGGKVSGQGGAAELLRLNAKTLYSRIDKLGIRKRYGAG

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=645, Percent_Identity=35.8139534883721, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI87082117, Length=405, Percent_Identity=45.1851851851852, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1788550, Length=338, Percent_Identity=44.3786982248521, Blast_Score=272, Evalue=5e-74,
Organism=Escherichia coli, GI1790437, Length=312, Percent_Identity=47.1153846153846, Blast_Score=272, Evalue=6e-74,
Organism=Escherichia coli, GI87082152, Length=321, Percent_Identity=45.4828660436137, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=40.7407407407407, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1788905, Length=234, Percent_Identity=48.7179487179487, Blast_Score=233, Evalue=4e-62,
Organism=Escherichia coli, GI1790299, Length=333, Percent_Identity=39.6396396396396, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=48.2758620689655, Blast_Score=207, Evalue=3e-54,
Organism=Escherichia coli, GI1786524, Length=263, Percent_Identity=45.2471482889734, Blast_Score=205, Evalue=8e-54,
Organism=Escherichia coli, GI1787583, Length=271, Percent_Identity=40.2214022140221, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081858, Length=291, Percent_Identity=33.6769759450172, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789828, Length=227, Percent_Identity=40.0881057268723, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78488; Mature: 78357

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTHLPEALRLMQYIGDAIGTIRDPQELFRTVTDKLRLIFPFDSAVIITLDRDRRRVSV
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEECCCEEEEEECCCCHHHHH
FFEMLRFELPDHLRVQRRPITGTWLEAHLDDRGVTVASIAHDIPAFGEEDAPLLRTLHEL
HHHHHHHCCCHHHEEECCCCCCCHHEEECCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHH
GMRQIVLSPLRSGGRVIGFLSFVSAEEKLWSDGDKSLLSGVSSSIAIAVSNALAYDELRQ
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHCCCHHHEEHHHHHHHHHHHHH
REAETAMQLAINNALFTTKDRSQMLLTVCEQISRLVPCSFLGIRVVGSDGRFRIYDNFMR
HHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEHHHC
GSGSSFAPFSPLEHLEMLPDDPVARESIEVISRPGIYSGERFEELCRDFRILELVRDRFG
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
ISSIIVVQLWDLPGSRAGLIISGVGVTLGDEEARTVSLIVPQLALALQNYLAFDEIDRLR
CCEEEEEEEECCCCCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKLEGERTYLVEEIRAAHNFEEIVGNSAPLAEVLRRVSLVAPTDATVLIEGETGTGKELI
HHHCCCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHEECCCCCEEEEECCCCCCHHHH
ARAIHNLSPRKERVLVKVNCAALPASLIESELFGHEKGSFTGATERRIGKFELADGGTIF
HHHHHCCCCCCCEEEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCCCEEE
LDEIGELPLELQAKLLRVLQEKELERIGGRSVIPVDVRVIAATNRDLEKEVATGRFRQDL
ECHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHH
YFRLNAFPLSVPPLRERRDDIPVLAMYFARKFAREFGKPERAIREHDMNELVSREWRGNI
HEEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHCCCH
RELSHTIEQAVIVSEGDTLDLSSALPPRGEPGMEAQAEVMTMQEFEEQARNMERKLILDA
HHHHHHHHHHEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRAGGKVSGQGGAAELLRLNAKTLYSRIDKLGIRKRYGAG
HHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
TTTHLPEALRLMQYIGDAIGTIRDPQELFRTVTDKLRLIFPFDSAVIITLDRDRRRVSV
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEECCCEEEEEECCCCHHHHH
FFEMLRFELPDHLRVQRRPITGTWLEAHLDDRGVTVASIAHDIPAFGEEDAPLLRTLHEL
HHHHHHHCCCHHHEEECCCCCCCHHEEECCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHH
GMRQIVLSPLRSGGRVIGFLSFVSAEEKLWSDGDKSLLSGVSSSIAIAVSNALAYDELRQ
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHCCCHHHEEHHHHHHHHHHHHH
REAETAMQLAINNALFTTKDRSQMLLTVCEQISRLVPCSFLGIRVVGSDGRFRIYDNFMR
HHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEHHHC
GSGSSFAPFSPLEHLEMLPDDPVARESIEVISRPGIYSGERFEELCRDFRILELVRDRFG
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
ISSIIVVQLWDLPGSRAGLIISGVGVTLGDEEARTVSLIVPQLALALQNYLAFDEIDRLR
CCEEEEEEEECCCCCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKLEGERTYLVEEIRAAHNFEEIVGNSAPLAEVLRRVSLVAPTDATVLIEGETGTGKELI
HHHCCCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHEECCCCCEEEEECCCCCCHHHH
ARAIHNLSPRKERVLVKVNCAALPASLIESELFGHEKGSFTGATERRIGKFELADGGTIF
HHHHHCCCCCCCEEEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCCCEEE
LDEIGELPLELQAKLLRVLQEKELERIGGRSVIPVDVRVIAATNRDLEKEVATGRFRQDL
ECHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHH
YFRLNAFPLSVPPLRERRDDIPVLAMYFARKFAREFGKPERAIREHDMNELVSREWRGNI
HEEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHCCCH
RELSHTIEQAVIVSEGDTLDLSSALPPRGEPGMEAQAEVMTMQEFEEQARNMERKLILDA
HHHHHHHHHHEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRAGGKVSGQGGAAELLRLNAKTLYSRIDKLGIRKRYGAG
HHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]