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The map label for this gene is pckG [H]

Identifier: 193211722

GI number: 193211722

Start: 52062

End: 53927

Strand: Direct

Name: pckG [H]

Synonym: Cpar_0047

Alternate gene names: 193211722

Gene position: 52062-53927 (Clockwise)

Preceding gene: 193211721

Following gene: 193211723

Centisome position: 2.27

GC content: 60.77

Gene sequence:

>1866_bases
ATGGAACCTATCCCAATCAATGCGCCGGAGTACGTCAAAAACCTGAAACTTCTCCAATGGGTCAAAGAGACCGTCGAGCT
GTGCCAGCCCGATTCGGTTTACTGGTGTGACGGTTCCACCGAAGAGTACGACCGCCTTTGCAATGAAATGGTCGAGAGCG
GCACTTTTATCAGACTCTCTGAAGAGAAGCGGCCGAACAGCTTCCTCTGCCGCTCCGACCCCAGCGACGTGGCCCGCGTC
GAGGATCGAACCTTTATTTGCAGCATTCGCCGCCAGGACGCCGGCCCGACCAACAACTGGGTCGCACCGAAGGAGATGAA
GGCGACGCTGAACAAGCTTTTCGCAGGCTGCATGAAGGGGCGCACCATGTACGTCATTCCCTTCAGCATGGGGCCGCTCG
GCTCGCATATCGCCCACATCGGCGTCGAAATCACCGACTCGCCCTACGTGGTGACCAACATGCGCATCATGACCCGCATG
GGCCGCAAGGTGCTCGAACTGCTCAGCGAGGAGGATGAGTTCGTGCCGTGCCTGCACTCGGTCGGTGCGCCACTCGAACC
GGGCCAGCAGGATGTGCCGTGGCCCTGCAACGATACCAAGTACATCGTCCACTTCCCGGAAGAGCGCGCCATCGTCTCCT
TCGGCAGTGGCTATGGCGGCAACGCCTTGCTCGGCAAAAAATGCTTCGCCCTGCGCATCGCCTCCTCGATGGCGCGTGAC
GAGGGGTGGCTTGCCGAGCACATGCTCATTCTCGGCGTCGAGTCGCCGGAGGGGGAAAAGGATTACGTCGGGGCTGCCTT
CCCGAGCGCCTGCGGCAAGACCAACTTCGCCATGATGATTCCGCCAGGCGAAATGGAGGGGTGGAAGATCACCACCGTTG
GCGACGATATCGCCTGGATCAAACAGGGTAAGGATGGCAAGCTGCACGCCATCAACCCCGAGTACGGCTTTTTCGGCGTC
GCACCGGGCACCTCGGAGAAGAGCAATCCGAACGCGATGGCGTCGTTGCACGCCAACTGCATCTTTACCAACGTGGCGAT
GACCCCCGACGGTGATGTGTGGTGGGAGGGGATGACCGATACGCCACCCGATTACCTGACCGATTGGCAGGGCAAGCCCT
GGGTACCGGGGTGCGACCGTCCTGCCGCCCATCCGAACGCCCGCTTCACGGCTCCGGCCCATCAGTGCCCGGTCATCGAC
GAGAACTGGGAAAACCCGGACGGCGTGCCGATTTCAGCCTTCATTTTCGGCGGACGGCGCGGCGATACCATTCCGCTGGT
CTACCAGTCGGCCAACTGGTACTACGGCGTCTATCTTGCCGCCACGATGGGCTCGGAGAAGACCGCCGCCGCCGCCGGCA
AGATCGGCGACGTGCGCCGCGACCCCTTCGCCATGCTGCCCTTCTGTGGCTACCACATGGGCGACTACTTCAACCACTGG
CTGCACGTTGGCCGCACGCTCACCGATCCGCCGCGAATTTTCGGCGTCAACTGGTTCCGCAAGGATGAAAACGGCAAGTT
CCTCTGGCCCGGATTCGGCGAGAACATGCGCGTGCTGAAGTGGATTATTGGTCGGGTGCATGGCCGCGCCGGTGGCGTCG
AAAGCCCACTTGGCTGGATGCCTCGCTACGAATCGATCGACTGGCGCGGGCTCGACGATTTCACCATCGAAAAGTTCACC
AAACTCATGTCGGTTGACCGTGAAGCTTGGAAGCAGGAGCTCTTCTCGCACGAGGAGCTGCTTGAGAAGCTCTACGACAA
GCTGCCGAAAGAGTTTACCCATATCCGCGAGCTGATGCTTTCAACGCTCTGGATGTCTCCCGAGCACTGGGAAATGGCTC
CTGAGCGCTACGCCATCTATCACTGA

Upstream 100 bases:

>100_bases
TGAGATTTTTCACCGCGCCAGCCCCGGAGGAGTGTTCCTGCCGGGGCGCGCTGTTTTACAGGATTTTATCTACACTTTTT
TCAATTGATCATCAGTCATT

Downstream 100 bases:

>100_bases
GCGGCCAAGCGTTTCATACAATCAAAAAAGCTGCCCCGGTCTCGTTTCCGGGGCAGCTTTTTTTTGTGGCGTATTTTCCT
CAACTGTTTTTGTTTTTGCT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MEPIPINAPEYVKNLKLLQWVKETVELCQPDSVYWCDGSTEEYDRLCNEMVESGTFIRLSEEKRPNSFLCRSDPSDVARV
EDRTFICSIRRQDAGPTNNWVAPKEMKATLNKLFAGCMKGRTMYVIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRM
GRKVLELLSEEDEFVPCLHSVGAPLEPGQQDVPWPCNDTKYIVHFPEERAIVSFGSGYGGNALLGKKCFALRIASSMARD
EGWLAEHMLILGVESPEGEKDYVGAAFPSACGKTNFAMMIPPGEMEGWKITTVGDDIAWIKQGKDGKLHAINPEYGFFGV
APGTSEKSNPNAMASLHANCIFTNVAMTPDGDVWWEGMTDTPPDYLTDWQGKPWVPGCDRPAAHPNARFTAPAHQCPVID
ENWENPDGVPISAFIFGGRRGDTIPLVYQSANWYYGVYLAATMGSEKTAAAAGKIGDVRRDPFAMLPFCGYHMGDYFNHW
LHVGRTLTDPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIIGRVHGRAGGVESPLGWMPRYESIDWRGLDDFTIEKFT
KLMSVDREAWKQELFSHEELLEKLYDKLPKEFTHIRELMLSTLWMSPEHWEMAPERYAIYH

Sequences:

>Translated_621_residues
MEPIPINAPEYVKNLKLLQWVKETVELCQPDSVYWCDGSTEEYDRLCNEMVESGTFIRLSEEKRPNSFLCRSDPSDVARV
EDRTFICSIRRQDAGPTNNWVAPKEMKATLNKLFAGCMKGRTMYVIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRM
GRKVLELLSEEDEFVPCLHSVGAPLEPGQQDVPWPCNDTKYIVHFPEERAIVSFGSGYGGNALLGKKCFALRIASSMARD
EGWLAEHMLILGVESPEGEKDYVGAAFPSACGKTNFAMMIPPGEMEGWKITTVGDDIAWIKQGKDGKLHAINPEYGFFGV
APGTSEKSNPNAMASLHANCIFTNVAMTPDGDVWWEGMTDTPPDYLTDWQGKPWVPGCDRPAAHPNARFTAPAHQCPVID
ENWENPDGVPISAFIFGGRRGDTIPLVYQSANWYYGVYLAATMGSEKTAAAAGKIGDVRRDPFAMLPFCGYHMGDYFNHW
LHVGRTLTDPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIIGRVHGRAGGVESPLGWMPRYESIDWRGLDDFTIEKFT
KLMSVDREAWKQELFSHEELLEKLYDKLPKEFTHIRELMLSTLWMSPEHWEMAPERYAIYH
>Mature_621_residues
MEPIPINAPEYVKNLKLLQWVKETVELCQPDSVYWCDGSTEEYDRLCNEMVESGTFIRLSEEKRPNSFLCRSDPSDVARV
EDRTFICSIRRQDAGPTNNWVAPKEMKATLNKLFAGCMKGRTMYVIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRM
GRKVLELLSEEDEFVPCLHSVGAPLEPGQQDVPWPCNDTKYIVHFPEERAIVSFGSGYGGNALLGKKCFALRIASSMARD
EGWLAEHMLILGVESPEGEKDYVGAAFPSACGKTNFAMMIPPGEMEGWKITTVGDDIAWIKQGKDGKLHAINPEYGFFGV
APGTSEKSNPNAMASLHANCIFTNVAMTPDGDVWWEGMTDTPPDYLTDWQGKPWVPGCDRPAAHPNARFTAPAHQCPVID
ENWENPDGVPISAFIFGGRRGDTIPLVYQSANWYYGVYLAATMGSEKTAAAAGKIGDVRRDPFAMLPFCGYHMGDYFNHW
LHVGRTLTDPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIIGRVHGRAGGVESPLGWMPRYESIDWRGLDDFTIEKFT
KLMSVDREAWKQELFSHEELLEKLYDKLPKEFTHIRELMLSTLWMSPEHWEMAPERYAIYH

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=587, Percent_Identity=52.8109028960818, Blast_Score=604, Evalue=1e-173,
Organism=Homo sapiens, GI187281517, Length=596, Percent_Identity=51.6778523489933, Blast_Score=602, Evalue=1e-172,
Organism=Homo sapiens, GI66346723, Length=371, Percent_Identity=54.7169811320755, Blast_Score=397, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71989645, Length=592, Percent_Identity=49.4932432432432, Blast_Score=568, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71989658, Length=592, Percent_Identity=49.4932432432432, Blast_Score=566, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI17555492, Length=593, Percent_Identity=49.4097807757167, Blast_Score=560, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71989653, Length=579, Percent_Identity=49.9136442141624, Blast_Score=558, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI32564411, Length=537, Percent_Identity=52.5139664804469, Blast_Score=546, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564407, Length=501, Percent_Identity=52.0958083832335, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71989649, Length=494, Percent_Identity=50.6072874493927, Blast_Score=491, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI32564409, Length=346, Percent_Identity=48.5549132947977, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17540956, Length=614, Percent_Identity=24.2671009771987, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24655085, Length=567, Percent_Identity=53.6155202821869, Blast_Score=611, Evalue=1e-175,
Organism=Drosophila melanogaster, GI24655082, Length=579, Percent_Identity=52.5043177892919, Blast_Score=587, Evalue=1e-168,
Organism=Drosophila melanogaster, GI161077226, Length=548, Percent_Identity=52.9197080291971, Blast_Score=558, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24655088, Length=472, Percent_Identity=55.7203389830508, Blast_Score=532, Evalue=1e-151,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 70183; Mature: 70183

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIPINAPEYVKNLKLLQWVKETVELCQPDSVYWCDGSTEEYDRLCNEMVESGTFIRLS
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEE
EEKRPNSFLCRSDPSDVARVEDRTFICSIRRQDAGPTNNWVAPKEMKATLNKLFAGCMKG
CCCCCCCEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RTMYVIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRMGRKVLELLSEEDEFVPCLHS
CEEEEEEECCCCCHHHHEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VGAPLEPGQQDVPWPCNDTKYIVHFPEERAIVSFGSGYGGNALLGKKCFALRIASSMARD
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
EGWLAEHMLILGVESPEGEKDYVGAAFPSACGKTNFAMMIPPGEMEGWKITTVGDDIAWI
CCHHEEEEEEEEECCCCCCCCCCCCCCCHHCCCCCEEEEECCCCCCCEEEEEECCCCHHH
KQGKDGKLHAINPEYGFFGVAPGTSEKSNPNAMASLHANCIFTNVAMTPDGDVWWEGMTD
CCCCCCCEEEECCCCCEEECCCCCCCCCCCCHHHHEECEEEEEEEEECCCCCEEECCCCC
TPPDYLTDWQGKPWVPGCDRPAAHPNARFTAPAHQCPVIDENWENPDGVPISAFIFGGRR
CCCHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
GDTIPLVYQSANWYYGVYLAATMGSEKTAAAAGKIGDVRRDPFAMLPFCGYHMGDYFNHW
CCEEEEEEECCCEEEEEEEEEECCCCHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHH
LHVGRTLTDPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIIGRVHGRAGGVESPLGWM
HHHCCCCCCCCCEEECEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
PRYESIDWRGLDDFTIEKFTKLMSVDREAWKQELFSHEELLEKLYDKLPKEFTHIRELML
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLWMSPEHWEMAPERYAIYH
HHHHCCCHHHCCCCCCEECCC
>Mature Secondary Structure
MEPIPINAPEYVKNLKLLQWVKETVELCQPDSVYWCDGSTEEYDRLCNEMVESGTFIRLS
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEE
EEKRPNSFLCRSDPSDVARVEDRTFICSIRRQDAGPTNNWVAPKEMKATLNKLFAGCMKG
CCCCCCCEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RTMYVIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRMGRKVLELLSEEDEFVPCLHS
CEEEEEEECCCCCHHHHEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VGAPLEPGQQDVPWPCNDTKYIVHFPEERAIVSFGSGYGGNALLGKKCFALRIASSMARD
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
EGWLAEHMLILGVESPEGEKDYVGAAFPSACGKTNFAMMIPPGEMEGWKITTVGDDIAWI
CCHHEEEEEEEEECCCCCCCCCCCCCCCHHCCCCCEEEEECCCCCCCEEEEEECCCCHHH
KQGKDGKLHAINPEYGFFGVAPGTSEKSNPNAMASLHANCIFTNVAMTPDGDVWWEGMTD
CCCCCCCEEEECCCCCEEECCCCCCCCCCCCHHHHEECEEEEEEEEECCCCCEEECCCCC
TPPDYLTDWQGKPWVPGCDRPAAHPNARFTAPAHQCPVIDENWENPDGVPISAFIFGGRR
CCCHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
GDTIPLVYQSANWYYGVYLAATMGSEKTAAAAGKIGDVRRDPFAMLPFCGYHMGDYFNHW
CCEEEEEEECCCEEEEEEEEEECCCCHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHH
LHVGRTLTDPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIIGRVHGRAGGVESPLGWM
HHHCCCCCCCCCEEECEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
PRYESIDWRGLDDFTIEKFTKLMSVDREAWKQELFSHEELLEKLYDKLPKEFTHIRELML
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLWMSPEHWEMAPERYAIYH
HHHHCCCHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]