Click here to switch to the map view.

The map label for this gene is tusE [H]

Identifier: 193211702

GI number: 193211702

Start: 32810

End: 33145

Strand: Direct

Name: tusE [H]

Synonym: Cpar_0027

Alternate gene names: 193211702

Gene position: 32810-33145 (Clockwise)

Preceding gene: 193211701

Following gene: 193211703

Centisome position: 1.43

GC content: 55.95

Gene sequence:

>336_bases
ATGGCAATTGAAGTCAACGGCATGAGCTACGAGACCGACGAGAACGGCTACCTTGTAAACCTCGAAGACTGGAACGAGGA
TGTAGCGGTAAAGCTCGCTGAAGGCGAGGAGATCTCGATGGAAGAGGGGCACTGGGACCTCGTGAAGTTCCTGAGGAACT
ACTACCAGGAGTACCAGATCGCTCCGGCCGTGAAGGTGCTTACCAAGGCTGTCGCTTCCGAGAAGGGCCTGGACAAGAAA
GAGGCTTCGGAGTTCCTGTACGGTCTTTTCCCCAAAGGCCCGGCTCTGCAGGCTTGCAAAATTGCCGGACTCCCCAAACC
GACCGGCTGCGTTTAA

Upstream 100 bases:

>100_bases
ATATAACCATATAACGATATTAATTGTCAGCTGCAGGGCTTTGCCAGAACGGCGAGCGCCTGCCGACAACCTCAACACCA
CACCAAAAAGGAGAACTATC

Downstream 100 bases:

>100_bases
GACGACGGCTACCCACAGAAACCAGGCCGGCGCAAGCCGGCCTCCAAAGTATCGAGAACCAAAGGAGGCAACATGAGTGG
TGCTAACGACAGCGCTATGA

Product: TusE/DsrC/DsvC family sulfur relay protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein E [H]

Number of amino acids: Translated: 111; Mature: 110

Protein sequence:

>111_residues
MAIEVNGMSYETDENGYLVNLEDWNEDVAVKLAEGEEISMEEGHWDLVKFLRNYYQEYQIAPAVKVLTKAVASEKGLDKK
EASEFLYGLFPKGPALQACKIAGLPKPTGCV

Sequences:

>Translated_111_residues
MAIEVNGMSYETDENGYLVNLEDWNEDVAVKLAEGEEISMEEGHWDLVKFLRNYYQEYQIAPAVKVLTKAVASEKGLDKK
EASEFLYGLFPKGPALQACKIAGLPKPTGCV
>Mature_110_residues
AIEVNGMSYETDENGYLVNLEDWNEDVAVKLAEGEEISMEEGHWDLVKFLRNYYQEYQIAPAVKVLTKAVASEKGLDKKE
ASEFLYGLFPKGPALQACKIAGLPKPTGCV

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Could accept sulfur from tusD [H]

COG id: COG2920

COG function: function code P; Dissimilatory sulfite reductase (desulfoviridin), gamma subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrC/tusE family [H]

Homologues:

Organism=Escherichia coli, GI87081811, Length=105, Percent_Identity=49.5238095238095, Blast_Score=110, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007453 [H]

Pfam domain/function: PF04358 DsrC [H]

EC number: NA

Molecular weight: Translated: 12346; Mature: 12215

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEVNGMSYETDENGYLVNLEDWNEDVAVKLAEGEEISMEEGHWDLVKFLRNYYQEYQI
CEEECCCCEEEECCCCEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
APAVKVLTKAVASEKGLDKKEASEFLYGLFPKGPALQACKIAGLPKPTGCV
CHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
AIEVNGMSYETDENGYLVNLEDWNEDVAVKLAEGEEISMEEGHWDLVKFLRNYYQEYQI
EEECCCCEEEECCCCEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
APAVKVLTKAVASEKGLDKKEASEFLYGLFPKGPALQACKIAGLPKPTGCV
CHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]