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The map label for this gene is serS

Identifier: 192291558

GI number: 192291558

Start: 3432310

End: 3433614

Strand: Reverse

Name: serS

Synonym: Rpal_3185

Alternate gene names: 192291558

Gene position: 3433614-3432310 (Counterclockwise)

Preceding gene: 192291559

Following gene: 192291557

Centisome position: 59.78

GC content: 65.98

Gene sequence:

>1305_bases
ATGCACGACATCAAAGCGATCCGCGACAATCCGCAGGCCTTCGACGCGGCGTTCACGAGGCGCGGTCTGGCGCCGATCGC
AGGTAGCCTGATGAAGCTCGACGAAGTCCGCCGCGCGGCGGTGCAGGCCGCCGAGCAGGCGCAGGCGCGGCGTAATGCGG
CGTCGAAAGAGATCGGCGACGCCAAGAAGGCCAAGGACAACGCCCGCGCCGAGGCGCTGATGGCCGAGGTCACCGAACTA
AAGACCACGATGCCGGCGCTCGACGCGGCGGTGAAGGAAGCCGACGAGGCGCTGAAAAAGGCGCTGTCGGAAATCCCGAA
CCTGCCGCTGCCAGAGGTGCCGGAAGGCGCCGACGAACACGGCAACGTCGAGCACCACCGCTTCGGCGAAAAGCCGAGCT
ACAGTTTCACGCCGAAGCCACATTACGACCTCGGCGAAGCGCTCGGCATGATGGACTTCGAAGCCGCGGCCAAACTCTCC
GGCGCGCGCTTCACCGTGCTGAAGAAGGGCCTCGCGCGGCTCGAACGCGCGATCGGTCAGTTTTTCCTCGACGTCCACAC
CGGCGACCACGGCTACACCGAAGTCAATCCGCCGCTGCTGGTGAAGGACGACGCGATGTTCGGTACCGCTCAGCTGCCGA
AGTTTCGCGAGGATCAGTTCGCGGCCGGAGCGATTGGCAGCGGTGGCGAGGGTTACTGGCTGATCCCCACCGCCGAAGTC
TCACTGACCAATCTCGTTCGCGAGTCCATCCTCGACGAGAAGGAGCTGCCGATGCGCCTCACAGCGTTGACGCCGTGCTT
CCGCGCTGAGGCGGGCGCTGCGGGGCGTGATACCCGCGGCATGATCCGGCAGCATCAGTTCACCAAGGTCGAGCTGGTGT
CGATCACGACGCCCGAGCAGAGCAAGGACGAGCACGAGCGGATGCTGTCCTGCGCCGAGGAAGTGCTGCGCCGGCTCGGG
CTGCATTACCGGGTGATGACGCTGTGCACCGGCGACATGGGCTTTGCGTCGCAGAAGACTTACGACATCGAAGTCTGGAT
GCCGGGGCAGGGCGAGGGCGGTGCGTACCGCGAAATCTCCTCGTGCTCGGTGTGCGGCGACTTCCAGGCCCGCCGCATGG
ACGCGCGCTCGCGCGGTTCGGACGGCAAGCCGCGTTTCGTCCACACGCTGAACGGCTCCGGTACCGCCGTCGGCCGTGCG
CTGATCGCGGTGATCGAGAACTATCAGCAGGAAGACGGCTCGATCGCCGTCCCCGACGTGCTGCAGCCCTACATGGGCGG
GCTGAAGGTGATCGCGAAGGCGTAA

Upstream 100 bases:

>100_bases
TTGTCGTGCCCGGCCTTGTGCCGGGCATTGCCGTCTTCTACAGACGTTGCTCCGCGACACGCGGACGGCCGGGACGGGCC
CGGCCTGACGAGGCGATTCC

Downstream 100 bases:

>100_bases
GGCTGATGTCTTTCCCCGTCATTGCGAGCGCATCGAAGCAATCAACGGCCCCGTGCACGGTGCTGGATTGCTTCGTCGCT
GCGCTCCTCGCAATGACGGT

Product: seryl-tRNA synthetase

Products: NA

Alternate protein names: Serine--tRNA ligase; SerRS; Seryl-tRNA(Ser/Sec) synthetase

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MHDIKAIRDNPQAFDAAFTRRGLAPIAGSLMKLDEVRRAAVQAAEQAQARRNAASKEIGDAKKAKDNARAEALMAEVTEL
KTTMPALDAAVKEADEALKKALSEIPNLPLPEVPEGADEHGNVEHHRFGEKPSYSFTPKPHYDLGEALGMMDFEAAAKLS
GARFTVLKKGLARLERAIGQFFLDVHTGDHGYTEVNPPLLVKDDAMFGTAQLPKFREDQFAAGAIGSGGEGYWLIPTAEV
SLTNLVRESILDEKELPMRLTALTPCFRAEAGAAGRDTRGMIRQHQFTKVELVSITTPEQSKDEHERMLSCAEEVLRRLG
LHYRVMTLCTGDMGFASQKTYDIEVWMPGQGEGGAYREISSCSVCGDFQARRMDARSRGSDGKPRFVHTLNGSGTAVGRA
LIAVIENYQQEDGSIAVPDVLQPYMGGLKVIAKA

Sequences:

>Translated_434_residues
MHDIKAIRDNPQAFDAAFTRRGLAPIAGSLMKLDEVRRAAVQAAEQAQARRNAASKEIGDAKKAKDNARAEALMAEVTEL
KTTMPALDAAVKEADEALKKALSEIPNLPLPEVPEGADEHGNVEHHRFGEKPSYSFTPKPHYDLGEALGMMDFEAAAKLS
GARFTVLKKGLARLERAIGQFFLDVHTGDHGYTEVNPPLLVKDDAMFGTAQLPKFREDQFAAGAIGSGGEGYWLIPTAEV
SLTNLVRESILDEKELPMRLTALTPCFRAEAGAAGRDTRGMIRQHQFTKVELVSITTPEQSKDEHERMLSCAEEVLRRLG
LHYRVMTLCTGDMGFASQKTYDIEVWMPGQGEGGAYREISSCSVCGDFQARRMDARSRGSDGKPRFVHTLNGSGTAVGRA
LIAVIENYQQEDGSIAVPDVLQPYMGGLKVIAKA
>Mature_434_residues
MHDIKAIRDNPQAFDAAFTRRGLAPIAGSLMKLDEVRRAAVQAAEQAQARRNAASKEIGDAKKAKDNARAEALMAEVTEL
KTTMPALDAAVKEADEALKKALSEIPNLPLPEVPEGADEHGNVEHHRFGEKPSYSFTPKPHYDLGEALGMMDFEAAAKLS
GARFTVLKKGLARLERAIGQFFLDVHTGDHGYTEVNPPLLVKDDAMFGTAQLPKFREDQFAAGAIGSGGEGYWLIPTAEV
SLTNLVRESILDEKELPMRLTALTPCFRAEAGAAGRDTRGMIRQHQFTKVELVSITTPEQSKDEHERMLSCAEEVLRRLG
LHYRVMTLCTGDMGFASQKTYDIEVWMPGQGEGGAYREISSCSVCGDFQARRMDARSRGSDGKPRFVHTLNGSGTAVGRA
LIAVIENYQQEDGSIAVPDVLQPYMGGLKVIAKA

Specific function: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily

Homologues:

Organism=Homo sapiens, GI16306548, Length=441, Percent_Identity=32.8798185941043, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI8923421, Length=375, Percent_Identity=32.2666666666667, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI225579074, Length=331, Percent_Identity=34.7432024169184, Blast_Score=193, Evalue=3e-49,
Organism=Escherichia coli, GI1787120, Length=434, Percent_Identity=50.6912442396313, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17539260, Length=456, Percent_Identity=31.7982456140351, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17542084, Length=356, Percent_Identity=26.685393258427, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320226, Length=398, Percent_Identity=31.1557788944724, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6321799, Length=436, Percent_Identity=30.2752293577982, Blast_Score=187, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24581334, Length=440, Percent_Identity=31.3636363636364, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI17737985, Length=369, Percent_Identity=32.520325203252, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYS_RHOPA (Q6N5X5)

Other databases:

- EMBL:   BX572602
- RefSeq:   NP_948186.1
- ProteinModelPortal:   Q6N5X5
- SMR:   Q6N5X5
- GeneID:   2693206
- GenomeReviews:   BX571963_GR
- KEGG:   rpa:RPA2845
- NMPDR:   fig|258594.1.peg.2829
- HOGENOM:   HBG629391
- OMA:   PKFADDM
- ProtClustDB:   PRK05431
- BioCyc:   RPAL258594:RPA2845-MONOMER
- BRENDA:   6.1.1.11
- GO:   GO:0005737
- HAMAP:   MF_00176
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR002317
- InterPro:   IPR015866
- InterPro:   IPR010978
- Gene3D:   G3DSA:1.10.287.40
- PANTHER:   PTHR11778
- PIRSF:   PIRSF001529
- PRINTS:   PR00981
- TIGRFAMs:   TIGR00414

Pfam domain/function: PF02403 Seryl_tRNA_N; PF00587 tRNA-synt_2b; SSF46589 tRNA_binding_arm

EC number: =6.1.1.11

Molecular weight: Translated: 47329; Mature: 47329

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 291-291 BINDING 393-393

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDIKAIRDNPQAFDAAFTRRGLAPIAGSLMKLDEVRRAAVQAAEQAQARRNAASKEIGD
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AKKAKDNARAEALMAEVTELKTTMPALDAAVKEADEALKKALSEIPNLPLPEVPEGADEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GNVEHHRFGEKPSYSFTPKPHYDLGEALGMMDFEAAAKLSGARFTVLKKGLARLERAIGQ
CCCHHHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
FFLDVHTGDHGYTEVNPPLLVKDDAMFGTAQLPKFREDQFAAGAIGSGGEGYWLIPTAEV
HEEEEECCCCCCEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHH
SLTNLVRESILDEKELPMRLTALTPCFRAEAGAAGRDTRGMIRQHQFTKVELVSITTPEQ
HHHHHHHHHHCCCCCCCEEHHHHCHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEEECCCC
SKDEHERMLSCAEEVLRRLGLHYRVMTLCTGDMGFASQKTYDIEVWMPGQGEGGAYREIS
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCHHHC
SCSVCGDFQARRMDARSRGSDGKPRFVHTLNGSGTAVGRALIAVIENYQQEDGSIAVPDV
CCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECHHH
LQPYMGGLKVIAKA
HHHHHCCHHHHCCC
>Mature Secondary Structure
MHDIKAIRDNPQAFDAAFTRRGLAPIAGSLMKLDEVRRAAVQAAEQAQARRNAASKEIGD
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AKKAKDNARAEALMAEVTELKTTMPALDAAVKEADEALKKALSEIPNLPLPEVPEGADEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GNVEHHRFGEKPSYSFTPKPHYDLGEALGMMDFEAAAKLSGARFTVLKKGLARLERAIGQ
CCCHHHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
FFLDVHTGDHGYTEVNPPLLVKDDAMFGTAQLPKFREDQFAAGAIGSGGEGYWLIPTAEV
HEEEEECCCCCCEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHH
SLTNLVRESILDEKELPMRLTALTPCFRAEAGAAGRDTRGMIRQHQFTKVELVSITTPEQ
HHHHHHHHHHCCCCCCCEEHHHHCHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEEECCCC
SKDEHERMLSCAEEVLRRLGLHYRVMTLCTGDMGFASQKTYDIEVWMPGQGEGGAYREIS
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCHHHC
SCSVCGDFQARRMDARSRGSDGKPRFVHTLNGSGTAVGRALIAVIENYQQEDGSIAVPDV
CCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECHHH
LQPYMGGLKVIAKA
HHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA