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The map label for this gene is bznB [H]

Identifier: 192291475

GI number: 192291475

Start: 3343682

End: 3345421

Strand: Reverse

Name: bznB [H]

Synonym: Rpal_3099

Alternate gene names: 192291475

Gene position: 3345421-3343682 (Counterclockwise)

Preceding gene: 192291477

Following gene: 192291474

Centisome position: 58.24

GC content: 66.38

Gene sequence:

>1740_bases
ATGGCAGAACAAGATCGCCTGGTCGGCGGACAATTGCTGGCAAAGACGCTGAAGGCCGCCGGTGTAAGCCAGGCGTTTGC
GCTGCACGGCGGACATCTCGAAGCGCTGCTCAAGGGGTGCATCGAGGAAGAGATTGCACTGATCGATTTCCGTCATGAGT
CGTCAGCCGGCCACGCTGCCGATGCGTATGCGCGTGCGACCGGCAAGCCCGGGGTATGCATCGTCACCGCCGGGCCAGGG
TTCACCAACGTACTGTCGGCGATGACCAATGCGCAGCTCGATGGCTCACCGGTGCTGTTCATCGTCGGCGCCCCGCCGCT
GCGTGAGGTGGAAACCAACCCGCTCCAGGGCGGGATCGATCAGGTTGCGATGGCTCGGCCGGCGGTGAAATGGGCGTTCT
CGATCCCCAGCACGGAGCGGATCGCCGATCTCACCGCGATGGCGATCCGCAAGGCGATGACGTTGCCGCGGGGCGCCGTG
CTGCTCGAAGTGCCGATCGACGTGCTGCATATGAGCGTCGCTGCTGCGCGCGCGACTCCTCCGGCCGGCGTCGGCGTCAA
TCCGCGCCCGGCGCCTGCCCCAGCTGAGGTCGCGCGATTAGTTGAACTGCTGCGGGCAGCCAAACGCCCTGTGCTGATCG
CCGGCAATGGCGCAGCCAATCATGAAACGGCGGAGGCGCTGCGAGCACTGTGCGCCAGGGTGCCGCTGCCGGTCTTCACC
AAGTCTCTGGCGGCCGGCATCCTGCCGCCGGGCCACCGCTGCAATGGCGGTGCGGCGGGCAACCTCGCCTTGTTGCCGGT
GCTGGGGATCGATCGGCCGGATCTCGTTATCCTGCTCGGCGGCAAACTCGGCCTGCTGCTCGGCGGACGTTCGGGCGCAC
TGGTGCCGCATGGCGCAACCCTGGTGCAGATCCACGGCGATGCGGCCGAGATGGGCCGGATCCGCGACGTCGATCTGGCC
ATCCTGGCGGATTGCACCGAGGCGACGCGCGCGCTCGACTCAGCTCTGAAGGACGGCCAGCTTGAGGAGATGGAGGCTTG
GCGGGCGCAGGCGGTCTCGGCAACCGGGCTGTTCGCGACGATGTTTCCCGAGCGCGAAACGACACGAGGCATTCATCCGT
ATCATGCGGCCCGCGCCGTCGCTGACGCGGCTGGGCCTGAGGCTGCCTATGTGTTCGATGGCGGGGAAGCGGCGTCGTGG
GGCGCCGCGGCAGCTGTCGTCGACCGGCCGGGAGCGCTGCTCGGTCACGGCTATCTCGGCTGCCTGGGGATCGGACCGGG
GTTCGCGATCGGCGCCCAGCTCGCCGCTCCGGAGCGCCGGGTGATCCACCTGACCGGTGACGGCGCGCTTGGATTCCACC
TGCAGGAACTCGACACCATGGTCCGGCACCGCTTGCCGATCGTCACCGTGGTTCTCAACAATGAAGTCTGGGGAATGTCG
ATCCACGGACAGCAAATCATGTTCGGCAGCAACTATCATGTGATCTCGAAGCTGGGCGGAACGCAGTTTGCCAACATCGC
TCAGGCCTTCGGCTGTCACGCCGAGCGTGTAACCCGGTTTGCCGACCTTGCGCCGGCGCTGGAGCGCGCCTTCGCCAGTG
GTGGCCCGGCCTTTATCGAGGTGATGACGGATGCCGACGTTGTGCATCCGGTCACGGTGGCGATGCTCGGTCAGGTTCAG
GAAGGCAGCAACGACGTGTTGATCCCCTACTACGAGAACATCCCCGCAGATACGGCCTGA

Upstream 100 bases:

>100_bases
CGCGTGCGCTCATTCATCCCCTTGTCCTCCCGGTTCGATGCTTATAATTATAGTGCACTATAATAACGGCGCGGCAGACG
CTGCAACAGGGAGGAATGAG

Downstream 100 bases:

>100_bases
GATGACGCTATCGACGAGCACTCTGGAATCCCGGCGGCGCGGGCTGGTGACAGCCGCGCACGCCTTGATCCGCGAATCCG
GCGGTGCCGGGTTTTCGATG

Product: thiamine pyrophosphate protein TPP binding domain-containing protein

Products: NA

Alternate protein names: Benzoin aldolase; BL; BZL [H]

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MAEQDRLVGGQLLAKTLKAAGVSQAFALHGGHLEALLKGCIEEEIALIDFRHESSAGHAADAYARATGKPGVCIVTAGPG
FTNVLSAMTNAQLDGSPVLFIVGAPPLREVETNPLQGGIDQVAMARPAVKWAFSIPSTERIADLTAMAIRKAMTLPRGAV
LLEVPIDVLHMSVAAARATPPAGVGVNPRPAPAPAEVARLVELLRAAKRPVLIAGNGAANHETAEALRALCARVPLPVFT
KSLAAGILPPGHRCNGGAAGNLALLPVLGIDRPDLVILLGGKLGLLLGGRSGALVPHGATLVQIHGDAAEMGRIRDVDLA
ILADCTEATRALDSALKDGQLEEMEAWRAQAVSATGLFATMFPERETTRGIHPYHAARAVADAAGPEAAYVFDGGEAASW
GAAAAVVDRPGALLGHGYLGCLGIGPGFAIGAQLAAPERRVIHLTGDGALGFHLQELDTMVRHRLPIVTVVLNNEVWGMS
IHGQQIMFGSNYHVISKLGGTQFANIAQAFGCHAERVTRFADLAPALERAFASGGPAFIEVMTDADVVHPVTVAMLGQVQ
EGSNDVLIPYYENIPADTA

Sequences:

>Translated_579_residues
MAEQDRLVGGQLLAKTLKAAGVSQAFALHGGHLEALLKGCIEEEIALIDFRHESSAGHAADAYARATGKPGVCIVTAGPG
FTNVLSAMTNAQLDGSPVLFIVGAPPLREVETNPLQGGIDQVAMARPAVKWAFSIPSTERIADLTAMAIRKAMTLPRGAV
LLEVPIDVLHMSVAAARATPPAGVGVNPRPAPAPAEVARLVELLRAAKRPVLIAGNGAANHETAEALRALCARVPLPVFT
KSLAAGILPPGHRCNGGAAGNLALLPVLGIDRPDLVILLGGKLGLLLGGRSGALVPHGATLVQIHGDAAEMGRIRDVDLA
ILADCTEATRALDSALKDGQLEEMEAWRAQAVSATGLFATMFPERETTRGIHPYHAARAVADAAGPEAAYVFDGGEAASW
GAAAAVVDRPGALLGHGYLGCLGIGPGFAIGAQLAAPERRVIHLTGDGALGFHLQELDTMVRHRLPIVTVVLNNEVWGMS
IHGQQIMFGSNYHVISKLGGTQFANIAQAFGCHAERVTRFADLAPALERAFASGGPAFIEVMTDADVVHPVTVAMLGQVQ
EGSNDVLIPYYENIPADTA
>Mature_578_residues
AEQDRLVGGQLLAKTLKAAGVSQAFALHGGHLEALLKGCIEEEIALIDFRHESSAGHAADAYARATGKPGVCIVTAGPGF
TNVLSAMTNAQLDGSPVLFIVGAPPLREVETNPLQGGIDQVAMARPAVKWAFSIPSTERIADLTAMAIRKAMTLPRGAVL
LEVPIDVLHMSVAAARATPPAGVGVNPRPAPAPAEVARLVELLRAAKRPVLIAGNGAANHETAEALRALCARVPLPVFTK
SLAAGILPPGHRCNGGAAGNLALLPVLGIDRPDLVILLGGKLGLLLGGRSGALVPHGATLVQIHGDAAEMGRIRDVDLAI
LADCTEATRALDSALKDGQLEEMEAWRAQAVSATGLFATMFPERETTRGIHPYHAARAVADAAGPEAAYVFDGGEAASWG
AAAAVVDRPGALLGHGYLGCLGIGPGFAIGAQLAAPERRVIHLTGDGALGFHLQELDTMVRHRLPIVTVVLNNEVWGMSI
HGQQIMFGSNYHVISKLGGTQFANIAQAFGCHAERVTRFADLAPALERAFASGGPAFIEVMTDADVVHPVTVAMLGQVQE
GSNDVLIPYYENIPADTA

Specific function: Cleavage of benzoin-anisoin acyloin linkage [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=557, Percent_Identity=30.5206463195691, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=23.8938053097345, Blast_Score=149, Evalue=8e-36,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=28.0289330922242, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=24.866785079929, Blast_Score=170, Evalue=3e-43,
Organism=Escherichia coli, GI1788716, Length=487, Percent_Identity=29.5687885010267, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1786717, Length=551, Percent_Identity=28.1306715063521, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=26.7399267399267, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=559, Percent_Identity=29.695885509839, Blast_Score=195, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17531299, Length=554, Percent_Identity=25.6317689530686, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=554, Percent_Identity=25.6317689530686, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=569, Percent_Identity=26.3620386643234, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6320816, Length=483, Percent_Identity=24.6376811594203, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323163, Length=506, Percent_Identity=24.703557312253, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323073, Length=526, Percent_Identity=23.0038022813688, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321524, Length=519, Percent_Identity=22.5433526011561, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922626, Length=480, Percent_Identity=28.125, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.2.38 [H]

Molecular weight: Translated: 60116; Mature: 59985

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQDRLVGGQLLAKTLKAAGVSQAFALHGGHLEALLKGCIEEEIALIDFRHESSAGHAA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCH
DAYARATGKPGVCIVTAGPGFTNVLSAMTNAQLDGSPVLFIVGAPPLREVETNPLQGGID
HHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
QVAMARPAVKWAFSIPSTERIADLTAMAIRKAMTLPRGAVLLEVPIDVLHMSVAAARATP
HHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCC
PAGVGVNPRPAPAPAEVARLVELLRAAKRPVLIAGNGAANHETAEALRALCARVPLPVFT
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHH
KSLAAGILPPGHRCNGGAAGNLALLPVLGIDRPDLVILLGGKLGLLLGGRSGALVPHGAT
HHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEEEECCCCCCCCCCCE
LVQIHGDAAEMGRIRDVDLAILADCTEATRALDSALKDGQLEEMEAWRAQAVSATGLFAT
EEEEECCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEE
MFPERETTRGIHPYHAARAVADAAGPEAAYVFDGGEAASWGAAAAVVDRPGALLGHGYLG
ECCCCHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCCCCEECCCCEE
CLGIGPGFAIGAQLAAPERRVIHLTGDGALGFHLQELDTMVRHRLPIVTVVLNNEVWGMS
EEECCCCHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEE
IHGQQIMFGSNYHVISKLGGTQFANIAQAFGCHAERVTRFADLAPALERAFASGGPAFIE
ECCCEEEECCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
VMTDADVVHPVTVAMLGQVQEGSNDVLIPYYENIPADTA
EECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
AEQDRLVGGQLLAKTLKAAGVSQAFALHGGHLEALLKGCIEEEIALIDFRHESSAGHAA
CCCCCCHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCH
DAYARATGKPGVCIVTAGPGFTNVLSAMTNAQLDGSPVLFIVGAPPLREVETNPLQGGID
HHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
QVAMARPAVKWAFSIPSTERIADLTAMAIRKAMTLPRGAVLLEVPIDVLHMSVAAARATP
HHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCC
PAGVGVNPRPAPAPAEVARLVELLRAAKRPVLIAGNGAANHETAEALRALCARVPLPVFT
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHH
KSLAAGILPPGHRCNGGAAGNLALLPVLGIDRPDLVILLGGKLGLLLGGRSGALVPHGAT
HHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEEEECCCCCCCCCCCE
LVQIHGDAAEMGRIRDVDLAILADCTEATRALDSALKDGQLEEMEAWRAQAVSATGLFAT
EEEEECCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEE
MFPERETTRGIHPYHAARAVADAAGPEAAYVFDGGEAASWGAAAAVVDRPGALLGHGYLG
ECCCCHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCCCCEECCCCEE
CLGIGPGFAIGAQLAAPERRVIHLTGDGALGFHLQELDTMVRHRLPIVTVVLNNEVWGMS
EEECCCCHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEE
IHGQQIMFGSNYHVISKLGGTQFANIAQAFGCHAERVTRFADLAPALERAFASGGPAFIE
ECCCEEEECCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
VMTDADVVHPVTVAMLGQVQEGSNDVLIPYYENIPADTA
EECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8026749 [H]