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The map label for this gene is gyrB [H]

Identifier: 192291144

GI number: 192291144

Start: 3006442

End: 3008493

Strand: Reverse

Name: gyrB [H]

Synonym: Rpal_2765

Alternate gene names: 192291144

Gene position: 3008493-3006442 (Counterclockwise)

Preceding gene: 192291147

Following gene: 192291141

Centisome position: 52.38

GC content: 65.64

Gene sequence:

>2052_bases
ATGGCCAAGGCATTGAAGGCAAGCTCGAAATCGAAATCCGGCGACGACCTGTTCGAAGGGGCGCCGAAGTCTGCCGCGCC
CAAGGCGCCGGCCAAGCCGAGCGGCGCCGAGGATGGTTATACCGCCGCGGACATCGAGGTTCTGGAGGGGCTTGAGCCGG
TCCGGCGCCGGCCCGGCATGTATATCGGCGGCACCGACGAGAAGGCGCTGCACCATCTGTTCGCCGAGGTGATCGACAAC
TCGATGGACGAGGCGCTGGCTGGCCACGCCACCTTCATCGAGGTCGAGCTGACGGCCGACGGCTTCCTCACCGTCTCTGA
TAACGGACGCGGCATTCCCGTTGATCCGCATCCGAAATTTCCGAAGAAGTCGGCGCTCGAAGTGATCATGTGCACGCTGC
ACGCCGGCGGCAAGTTCGACAGCAAGGTGTACGAGACCTCGGGCGGTCTGCACGGCGTCGGCGTCAGCGTCGTCAATGCG
TTGTCGTCGCGTCTTGAGGTCGAAGTGGCGCGCGGCCAGCAGCTCCACCGCATGGTGTTCGAGCGCGGCCACCCCAAGGG
CAAGCTCGAGAGCCTCGGCAAGGTCAACAACCGCCGCGGCACCCGCGTCCGCTTCAAGCCGGACACCGACATCTTCGGCG
CCAAGGCGGCGTTCAAGCCGCAGCGGCTGTTCAAGATGGCGCGGTCGAAGGCGTATCTGTTCGGCGGCGTGAAGATCCGT
TGGAAGTGCGATCCGGAACTGCTGAAGGGCATCGAGGACGTTCCGGCCGAGGATGAATTCCATTTTCCCGGTGGCCTGAA
GGACTTCCTCGGCGCGGCGATCCACAACGACACGCTGGTGCACCCGGACATCTTCGCCGGCAAATCCGGTCGCAATGGCG
CGCACGGCGCCTGCGAATGGGCGGTGGCCTGGACGGCCGATGCCGACGGCTTCCTGTCGTCCTACTGCAACACCGTCCCG
ACACCCGATGGCGGCACCCACGAGTCGGGCTTGCGCAGCGCCCTGCTCCGCGGTCTGAAGGACCACGCCGAACGTGCCGG
CCAGGGCAAGCGCGCCTCCTCGATCACCTCCGAAGACGTGATGGTCGGTGCTGCGATCATGCTCAGCGTGTTCGTGCGCG
AACCTGAATTCCAGGGCCAGACCAAGGACCGCCTCGCCACCGCCGAGGCGCAGAAGATCGTCGAACAGGCGATCAAGGAC
CCGTTCGACCACTGGCTGTCCGGCAATCCGGTCCAGGCCAACAAGCTGCTCGATTTCGTCATCGAGCGCGCCGAGGAGCG
GCTGCGCCGCCGCCAGGAAAAGGAAATCTCGCGCAAGACCGCGGTGAAGAAGCTGCGGCTGCCGGGCAAGCTGGCCGACT
GCACCTCGACCGCGGCCGAAGGCTCCGAACTGTTCATCGTCGAGGGCGACTCGGCCGGCGGCAGCGCCAAGCAGGCGCGC
GACCGTTCGACCCAGGCGGTGCTGCCGCTGCGCGGCAAGATCCTCAACGTCGCCTCCGCCGGCAAGGACAAGGTGGTGGC
GAACGCCCAGCTGTCGGACCTGATCCAGGCGCTCGGTGTCAGCACCGGGGTGAACTACCGCGAGGAAGATCTGCGCTATT
CGCGCATCATCATCATGACCGACGCCGACGTCGACGGCGCCCACATCGCCTCGCTGCTGATCACCTTCTTCTATCGGCAG
ATGCCGAAGCTGATCGACGAGGGCCACCTCTATCTCGCGGTGCCGCCGCTGTACCGCCTGACCCACGGCGCCAAGACGGT
GTACGCGCGCGACGACGCCCACAAGGAAGAGCTGCTGAAGAAGGAGTTCAATGCCAACGCCAAGGTCGAAGTCGGCCGCT
TCAAAGGCCTCGGCGAGATGATGCCGGCGCAGCTCAAGGAAACCACGATGGATCCGGCCAAGCGGACCATGCTACGCGTG
GTGCTGCTCCCCGACGACCGCGACGGCACCGCCAATTCGGTCGAGCGACTGATGGGCACCAAGGCCGAAGCCCGCTTCGC
CTTCATCTCCGAGAAGGCCGAATTCGCCAGCGACGAGCTGCTGGACGTGTGA

Upstream 100 bases:

>100_bases
GACCCAGCGTCACACCCGCAGCCCTTCCATGGCATAGTGCAACACAGCGATTCGTACGGCCTCAGCCGTTCGCACGACAT
CAGAACAGACTCCAGGACTC

Downstream 100 bases:

>100_bases
GGACTCGATCGGTCAGGCGGCGTCCGCCGGCCGCACCTGACGGTCAATCTCCGCGCCCTTGTCACGCTCGATGACGGCGA
TGTCGTACTGGCTCTGCAGA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MAKALKASSKSKSGDDLFEGAPKSAAPKAPAKPSGAEDGYTAADIEVLEGLEPVRRRPGMYIGGTDEKALHHLFAEVIDN
SMDEALAGHATFIEVELTADGFLTVSDNGRGIPVDPHPKFPKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVNA
LSSRLEVEVARGQQLHRMVFERGHPKGKLESLGKVNNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMARSKAYLFGGVKIR
WKCDPELLKGIEDVPAEDEFHFPGGLKDFLGAAIHNDTLVHPDIFAGKSGRNGAHGACEWAVAWTADADGFLSSYCNTVP
TPDGGTHESGLRSALLRGLKDHAERAGQGKRASSITSEDVMVGAAIMLSVFVREPEFQGQTKDRLATAEAQKIVEQAIKD
PFDHWLSGNPVQANKLLDFVIERAEERLRRRQEKEISRKTAVKKLRLPGKLADCTSTAAEGSELFIVEGDSAGGSAKQAR
DRSTQAVLPLRGKILNVASAGKDKVVANAQLSDLIQALGVSTGVNYREEDLRYSRIIIMTDADVDGAHIASLLITFFYRQ
MPKLIDEGHLYLAVPPLYRLTHGAKTVYARDDAHKEELLKKEFNANAKVEVGRFKGLGEMMPAQLKETTMDPAKRTMLRV
VLLPDDRDGTANSVERLMGTKAEARFAFISEKAEFASDELLDV

Sequences:

>Translated_683_residues
MAKALKASSKSKSGDDLFEGAPKSAAPKAPAKPSGAEDGYTAADIEVLEGLEPVRRRPGMYIGGTDEKALHHLFAEVIDN
SMDEALAGHATFIEVELTADGFLTVSDNGRGIPVDPHPKFPKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVNA
LSSRLEVEVARGQQLHRMVFERGHPKGKLESLGKVNNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMARSKAYLFGGVKIR
WKCDPELLKGIEDVPAEDEFHFPGGLKDFLGAAIHNDTLVHPDIFAGKSGRNGAHGACEWAVAWTADADGFLSSYCNTVP
TPDGGTHESGLRSALLRGLKDHAERAGQGKRASSITSEDVMVGAAIMLSVFVREPEFQGQTKDRLATAEAQKIVEQAIKD
PFDHWLSGNPVQANKLLDFVIERAEERLRRRQEKEISRKTAVKKLRLPGKLADCTSTAAEGSELFIVEGDSAGGSAKQAR
DRSTQAVLPLRGKILNVASAGKDKVVANAQLSDLIQALGVSTGVNYREEDLRYSRIIIMTDADVDGAHIASLLITFFYRQ
MPKLIDEGHLYLAVPPLYRLTHGAKTVYARDDAHKEELLKKEFNANAKVEVGRFKGLGEMMPAQLKETTMDPAKRTMLRV
VLLPDDRDGTANSVERLMGTKAEARFAFISEKAEFASDELLDV
>Mature_682_residues
AKALKASSKSKSGDDLFEGAPKSAAPKAPAKPSGAEDGYTAADIEVLEGLEPVRRRPGMYIGGTDEKALHHLFAEVIDNS
MDEALAGHATFIEVELTADGFLTVSDNGRGIPVDPHPKFPKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVNAL
SSRLEVEVARGQQLHRMVFERGHPKGKLESLGKVNNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMARSKAYLFGGVKIRW
KCDPELLKGIEDVPAEDEFHFPGGLKDFLGAAIHNDTLVHPDIFAGKSGRNGAHGACEWAVAWTADADGFLSSYCNTVPT
PDGGTHESGLRSALLRGLKDHAERAGQGKRASSITSEDVMVGAAIMLSVFVREPEFQGQTKDRLATAEAQKIVEQAIKDP
FDHWLSGNPVQANKLLDFVIERAEERLRRRQEKEISRKTAVKKLRLPGKLADCTSTAAEGSELFIVEGDSAGGSAKQARD
RSTQAVLPLRGKILNVASAGKDKVVANAQLSDLIQALGVSTGVNYREEDLRYSRIIIMTDADVDGAHIASLLITFFYRQM
PKLIDEGHLYLAVPPLYRLTHGAKTVYARDDAHKEELLKKEFNANAKVEVGRFKGLGEMMPAQLKETTMDPAKRTMLRVV
LLPDDRDGTANSVERLMGTKAEARFAFISEKAEFASDELLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=629, Percent_Identity=24.9602543720191, Blast_Score=145, Evalue=2e-34,
Organism=Homo sapiens, GI19913408, Length=648, Percent_Identity=24.2283950617284, Blast_Score=135, Evalue=2e-31,
Organism=Escherichia coli, GI1789408, Length=644, Percent_Identity=42.3913043478261, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI48994957, Length=562, Percent_Identity=45.1957295373665, Blast_Score=451, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17535065, Length=634, Percent_Identity=22.397476340694, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI212645845, Length=574, Percent_Identity=23.1707317073171, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=162, Percent_Identity=31.4814814814815, Blast_Score=75, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=656, Percent_Identity=24.5426829268293, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17136538, Length=654, Percent_Identity=23.5474006116208, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74378; Mature: 74247

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKALKASSKSKSGDDLFEGAPKSAAPKAPAKPSGAEDGYTAADIEVLEGLEPVRRRPGM
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCE
YIGGTDEKALHHLFAEVIDNSMDEALAGHATFIEVELTADGFLTVSDNGRGIPVDPHPKF
EECCCCHHHHHHHHHHHHHCHHHHHHCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCC
PKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVNALSSRLEVEVARGQQLHRMVF
CCHHHHHHHHEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
ERGHPKGKLESLGKVNNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMARSKAYLFGGVKIR
HCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEEECEEEE
WKCDPELLKGIEDVPAEDEFHFPGGLKDFLGAAIHNDTLVHPDIFAGKSGRNGAHGACEW
EECCHHHHCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCEEE
AVAWTADADGFLSSYCNTVPTPDGGTHESGLRSALLRGLKDHAERAGQGKRASSITSEDV
EEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
MVGAAIMLSVFVREPEFQGQTKDRLATAEAQKIVEQAIKDPFDHWLSGNPVQANKLLDFV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHH
IERAEERLRRRQEKEISRKTAVKKLRLPGKLADCTSTAAEGSELFIVEGDSAGGSAKQAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
DRSTQAVLPLRGKILNVASAGKDKVVANAQLSDLIQALGVSTGVNYREEDLRYSRIIIMT
CCCCCEEECCCCCEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCHHCCCEEEEEEEE
DADVDGAHIASLLITFFYRQMPKLIDEGHLYLAVPPLYRLTHGAKTVYARDDAHKEELLK
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCEEEECCCHHHHHHHH
KEFNANAKVEVGRFKGLGEMMPAQLKETTMDPAKRTMLRVVLLPDDRDGTANSVERLMGT
HHHCCCCEEEEHHHCCCHHHHHHHHHHHCCCHHHHEEEEEEEECCCCCCCHHHHHHHHCC
KAEARFAFISEKAEFASDELLDV
CHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
AKALKASSKSKSGDDLFEGAPKSAAPKAPAKPSGAEDGYTAADIEVLEGLEPVRRRPGM
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCE
YIGGTDEKALHHLFAEVIDNSMDEALAGHATFIEVELTADGFLTVSDNGRGIPVDPHPKF
EECCCCHHHHHHHHHHHHHCHHHHHHCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCC
PKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVNALSSRLEVEVARGQQLHRMVF
CCHHHHHHHHEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
ERGHPKGKLESLGKVNNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMARSKAYLFGGVKIR
HCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEEECEEEE
WKCDPELLKGIEDVPAEDEFHFPGGLKDFLGAAIHNDTLVHPDIFAGKSGRNGAHGACEW
EECCHHHHCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCEEE
AVAWTADADGFLSSYCNTVPTPDGGTHESGLRSALLRGLKDHAERAGQGKRASSITSEDV
EEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
MVGAAIMLSVFVREPEFQGQTKDRLATAEAQKIVEQAIKDPFDHWLSGNPVQANKLLDFV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHH
IERAEERLRRRQEKEISRKTAVKKLRLPGKLADCTSTAAEGSELFIVEGDSAGGSAKQAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
DRSTQAVLPLRGKILNVASAGKDKVVANAQLSDLIQALGVSTGVNYREEDLRYSRIIIMT
CCCCCEEECCCCCEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCHHCCCEEEEEEEE
DADVDGAHIASLLITFFYRQMPKLIDEGHLYLAVPPLYRLTHGAKTVYARDDAHKEELLK
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCEEEECCCHHHHHHHH
KEFNANAKVEVGRFKGLGEMMPAQLKETTMDPAKRTMLRVVLLPDDRDGTANSVERLMGT
HHHCCCCEEEEHHHCCCHHHHHHHHHHHCCCHHHHEEEEEEEECCCCCCCHHHHHHHHCC
KAEARFAFISEKAEFASDELLDV
CHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]