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The map label for this gene is menE [H]

Identifier: 192291011

GI number: 192291011

Start: 2847374

End: 2848639

Strand: Reverse

Name: menE [H]

Synonym: Rpal_2630

Alternate gene names: 192291011

Gene position: 2848639-2847374 (Counterclockwise)

Preceding gene: 192291012

Following gene: 192291010

Centisome position: 49.59

GC content: 64.85

Gene sequence:

>1266_bases
ATGATTCAGGTTGGCGACCGATTCTACGATCAAGCCATCGTGCAGACGATGGGCGCCCGCGCGGCTGAAGCAGTGGGCCT
CAGTGATCGACCCGAGCTGAGCTTTGCGGTCTGCCTGTCCGATCCGGCAGAATTCCTGGCGCTGCTACTGGCGATCCGCG
AAGCCGGCGCCAGCGTGCTGCCGATCCATCCCTCAACGCCGCGCGCTGCGGCACGCCGGCTGGCGATTGACCTTGGTTGC
CAACGGCTGGTGACCGATGAGCTCGCCATCGAGGATCTCGGCGGAGAAGACCCCGGCCCCGGCGGACTGCTGCAGACCAC
ATCCGGCACCACCGGCGAACCGAAGCGGATCGCCCGCAGTTGGGCGGAGATCGATCGCGAGATCGATAGCTACGTCGCAA
CTTTCGTCGCCCCGCAGGATATGACGCCGCTGATCGCATGTCCGATTACACATTCGTACGGACTGATCTGCGGCGTTCTG
GTCGCGCTCCGGCGGGGCCAAGTGCCGCACATTCTCGGTACGGCCAATCCGAAGTATCTCATCGCTCGCCTACGCCAGAC
CCGCCAGCCACTGTTGTATTCATCGCCCGCCATGCTCCACGCCATCGCCCGCCTGCTGCCGGCAGGCGAGATGATCCACG
CGGCGATGACCTCCGGCACTCTGCTGCCCGATCAGTGGCTGATGACGATCCGGGCCCGCACGACGCATCTGTTTCAGCAG
TATGGCTGCTCGGAAGCCGGATGCGTGGCGATCAATCCAAACCTGTCCGCATCAGGTGACATGGGAGCGCCGCTACCCCA
TCTCCGTGTCGAGGCCGGAGATACCGATCAGCCGCGGGAGATTGTGCTCCACGTCGGCGACCGCGCAATCCGGACCGCCG
ATCTCGGCTACACAAAAGCCGATGGCACGTTGGTGTTCGTCGGGCGCCTCGACGACACCATCAACGTCTCAGGCCTCAAT
GTCTATCCGAAGGAGGTCGAAGACGTGGTGGCGATGCCGGGCGTGACCGATGCGGTCGTATTCCGGCAGCTGGATTCGCT
CGCGGGCGAGCGGGTGGCCGTGGCGTTCAGCGCCGAGCACAGCATTGCGCCCGCGGACCTGCGCAACTGGTGTATCAAGA
ACCTGGCTGCCCATCAGGTCCCGATGGAGACCGTGCGCCTCCCCCGGCTGCCACGGCAGGCTAACGGCAAGATCAGCCGA
CGAGAGATCGCCGCCCTGCATGCTGAAGGACGCCTCGAGCTTTTGGCAGAGGAGACCGCCGCGTGA

Upstream 100 bases:

>100_bases
AAAGCCTGCTTGCCCGAAAACTCGGCCTGTGGGGCGACGCTCTCCGACACAAGGTGCCCAATCCACTTGCGGCGGTCGTG
CCGCTTGCACGGGAGTATGC

Downstream 100 bases:

>100_bases
CTCGCCAGGAGATCACGGCCGCGATTCACCAGGTGCTTGCCGAGCGGATGAACAACCGGCATCTCGACGGCTTTGGCCCA
CAATCGCGGCTGAATGCCGA

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MIQVGDRFYDQAIVQTMGARAAEAVGLSDRPELSFAVCLSDPAEFLALLLAIREAGASVLPIHPSTPRAAARRLAIDLGC
QRLVTDELAIEDLGGEDPGPGGLLQTTSGTTGEPKRIARSWAEIDREIDSYVATFVAPQDMTPLIACPITHSYGLICGVL
VALRRGQVPHILGTANPKYLIARLRQTRQPLLYSSPAMLHAIARLLPAGEMIHAAMTSGTLLPDQWLMTIRARTTHLFQQ
YGCSEAGCVAINPNLSASGDMGAPLPHLRVEAGDTDQPREIVLHVGDRAIRTADLGYTKADGTLVFVGRLDDTINVSGLN
VYPKEVEDVVAMPGVTDAVVFRQLDSLAGERVAVAFSAEHSIAPADLRNWCIKNLAAHQVPMETVRLPRLPRQANGKISR
REIAALHAEGRLELLAEETAA

Sequences:

>Translated_421_residues
MIQVGDRFYDQAIVQTMGARAAEAVGLSDRPELSFAVCLSDPAEFLALLLAIREAGASVLPIHPSTPRAAARRLAIDLGC
QRLVTDELAIEDLGGEDPGPGGLLQTTSGTTGEPKRIARSWAEIDREIDSYVATFVAPQDMTPLIACPITHSYGLICGVL
VALRRGQVPHILGTANPKYLIARLRQTRQPLLYSSPAMLHAIARLLPAGEMIHAAMTSGTLLPDQWLMTIRARTTHLFQQ
YGCSEAGCVAINPNLSASGDMGAPLPHLRVEAGDTDQPREIVLHVGDRAIRTADLGYTKADGTLVFVGRLDDTINVSGLN
VYPKEVEDVVAMPGVTDAVVFRQLDSLAGERVAVAFSAEHSIAPADLRNWCIKNLAAHQVPMETVRLPRLPRQANGKISR
REIAALHAEGRLELLAEETAA
>Mature_421_residues
MIQVGDRFYDQAIVQTMGARAAEAVGLSDRPELSFAVCLSDPAEFLALLLAIREAGASVLPIHPSTPRAAARRLAIDLGC
QRLVTDELAIEDLGGEDPGPGGLLQTTSGTTGEPKRIARSWAEIDREIDSYVATFVAPQDMTPLIACPITHSYGLICGVL
VALRRGQVPHILGTANPKYLIARLRQTRQPLLYSSPAMLHAIARLLPAGEMIHAAMTSGTLLPDQWLMTIRARTTHLFQQ
YGCSEAGCVAINPNLSASGDMGAPLPHLRVEAGDTDQPREIVLHVGDRAIRTADLGYTKADGTLVFVGRLDDTINVSGLN
VYPKEVEDVVAMPGVTDAVVFRQLDSLAGERVAVAFSAEHSIAPADLRNWCIKNLAAHQVPMETVRLPRLPRQANGKISR
REIAALHAEGRLELLAEETAA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=379, Percent_Identity=25.3298153034301, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=361, Percent_Identity=26.8698060941828, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581924, Length=360, Percent_Identity=24.4444444444444, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 45296; Mature: 45296

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQVGDRFYDQAIVQTMGARAAEAVGLSDRPELSFAVCLSDPAEFLALLLAIREAGASVL
CCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEE
PIHPSTPRAAARRLAIDLGCQRLVTDELAIEDLGGEDPGPGGLLQTTSGTTGEPKRIARS
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHH
WAEIDREIDSYVATFVAPQDMTPLIACPITHSYGLICGVLVALRRGQVPHILGTANPKYL
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHH
IARLRQTRQPLLYSSPAMLHAIARLLPAGEMIHAAMTSGTLLPDQWLMTIRARTTHLFQQ
HHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
YGCSEAGCVAINPNLSASGDMGAPLPHLRVEAGDTDQPREIVLHVGDRAIRTADLGYTKA
HCCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHEEEECCCCCEEEECCCEEEC
DGTLVFVGRLDDTINVSGLNVYPKEVEDVVAMPGVTDAVVFRQLDSLAGERVAVAFSAEH
CCEEEEEEECCCEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCC
SIAPADLRNWCIKNLAAHQVPMETVRLPRLPRQANGKISRREIAALHAEGRLELLAEETA
CCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEHHHHCC
A
C
>Mature Secondary Structure
MIQVGDRFYDQAIVQTMGARAAEAVGLSDRPELSFAVCLSDPAEFLALLLAIREAGASVL
CCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEE
PIHPSTPRAAARRLAIDLGCQRLVTDELAIEDLGGEDPGPGGLLQTTSGTTGEPKRIARS
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHH
WAEIDREIDSYVATFVAPQDMTPLIACPITHSYGLICGVLVALRRGQVPHILGTANPKYL
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHH
IARLRQTRQPLLYSSPAMLHAIARLLPAGEMIHAAMTSGTLLPDQWLMTIRARTTHLFQQ
HHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
YGCSEAGCVAINPNLSASGDMGAPLPHLRVEAGDTDQPREIVLHVGDRAIRTADLGYTKA
HCCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHEEEECCCCCEEEECCCEEEC
DGTLVFVGRLDDTINVSGLNVYPKEVEDVVAMPGVTDAVVFRQLDSLAGERVAVAFSAEH
CCEEEEEEECCCEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCC
SIAPADLRNWCIKNLAAHQVPMETVRLPRLPRQANGKISRREIAALHAEGRLELLAEETA
CCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]