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The map label for this gene is paaF [C]

Identifier: 192290942

GI number: 192290942

Start: 2765095

End: 2765880

Strand: Reverse

Name: paaF [C]

Synonym: Rpal_2559

Alternate gene names: 192290942

Gene position: 2765880-2765095 (Counterclockwise)

Preceding gene: 192290943

Following gene: 192290939

Centisome position: 48.15

GC content: 67.68

Gene sequence:

>786_bases
ATGACCGACACTCCTTACGTGCTGCGCGCCGATGACGACGGCGTCACGACGCTGACGCTGAACCGGCCGCAGGCGCGCAA
CGCGCTGTCGCAGGGCATGCTGGAGGCGCTGCGCGCGGCGCTGGCGGAGATCGCCGCCGACGCCAGCGTGCAGGTGCTGA
TCATCGCCGGCTCCGGCCCGGCCTTCTGCGCCGGTCATGACCTGAAGGAACTGCGCGCCGGCAACTACGCCCCGGACTAT
GTCGACGCCCTGTTCGCCGCCTGTGCGGAGGTGATGCAGGCGATCGTTCACCTGCCGAAGCCGGTGATCGCGCGCGTCCA
CGGCGTTGCCACCGCCGCAGGTGCCCAGCTGGTCGCCAGCGCCGACCTCGCCTTTGCCGCGGACGATGCGCGGTTCGCAA
CCCCGGGCGTCAACATCGGCCTGTTCTGCTCGACCCCGATGGTGGCGCTGTCGCGGAATCTGACGCGCAAGCACGCCATG
CAGATGCTGCTGTCGGGCGATCTGATCGACGCCGACACCGCATTCCGCTTCGGCCTGATCAACGAAGTCGTTCCCGCTGC
CGAGCTCGAACAGCGCACCCTCGCCTTCGCGCGGCAAATTGCGAGCAAATCCCCGCTGACGCTGGCGATCGGCAAAGAGG
CGTTCTACCGCCAGGCCGAACTGTCGCTGGCGGATGCCTACGCTTACACCAGCCAGGTGATGGCGAAGAACCTTCAGGCC
GAAGATGCCAAGGAAGGCATCGATGCCTTCCTTGGGAAGCGCCAGCCGGTGTGGTGCGGGCGGTGA

Upstream 100 bases:

>100_bases
GATCGCCGAGCCGACCGCCACCGAGCTGGCGAACCTGCGCAAGGTCGATGAGACCGGCGCGCTACGCAAGCACTAGCGCG
CCCGACAACGAGGACTTCCG

Downstream 100 bases:

>100_bases
GAAATCGCGGAGCGGAGGCTCCACGCGCGCATTAAAGCAGCCGTCCCCCGATCACATAGATCGGCGCCAGCACCACGAAC
GCCGCCACGAGAAGGCAAGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTDTPYVLRADDDGVTTLTLNRPQARNALSQGMLEALRAALAEIAADASVQVLIIAGSGPAFCAGHDLKELRAGNYAPDY
VDALFAACAEVMQAIVHLPKPVIARVHGVATAAGAQLVASADLAFAADDARFATPGVNIGLFCSTPMVALSRNLTRKHAM
QMLLSGDLIDADTAFRFGLINEVVPAAELEQRTLAFARQIASKSPLTLAIGKEAFYRQAELSLADAYAYTSQVMAKNLQA
EDAKEGIDAFLGKRQPVWCGR

Sequences:

>Translated_261_residues
MTDTPYVLRADDDGVTTLTLNRPQARNALSQGMLEALRAALAEIAADASVQVLIIAGSGPAFCAGHDLKELRAGNYAPDY
VDALFAACAEVMQAIVHLPKPVIARVHGVATAAGAQLVASADLAFAADDARFATPGVNIGLFCSTPMVALSRNLTRKHAM
QMLLSGDLIDADTAFRFGLINEVVPAAELEQRTLAFARQIASKSPLTLAIGKEAFYRQAELSLADAYAYTSQVMAKNLQA
EDAKEGIDAFLGKRQPVWCGR
>Mature_260_residues
TDTPYVLRADDDGVTTLTLNRPQARNALSQGMLEALRAALAEIAADASVQVLIIAGSGPAFCAGHDLKELRAGNYAPDYV
DALFAACAEVMQAIVHLPKPVIARVHGVATAAGAQLVASADLAFAADDARFATPGVNIGLFCSTPMVALSRNLTRKHAMQ
MLLSGDLIDADTAFRFGLINEVVPAAELEQRTLAFARQIASKSPLTLAIGKEAFYRQAELSLADAYAYTSQVMAKNLQAE
DAKEGIDAFLGKRQPVWCGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=49.1869918699187, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=31.4741035856574, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI70995211, Length=251, Percent_Identity=27.8884462151394, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI260274832, Length=250, Percent_Identity=25.2, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI45643119, Length=250, Percent_Identity=25.2, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260275230, Length=250, Percent_Identity=25.2, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4502327, Length=264, Percent_Identity=27.6515151515151, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4757966, Length=255, Percent_Identity=25.8823529411765, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538581, Length=255, Percent_Identity=25.8823529411765, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI25453481, Length=250, Percent_Identity=26.4, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI51538573, Length=250, Percent_Identity=26.4, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI221136756, Length=250, Percent_Identity=24.8, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221136753, Length=250, Percent_Identity=24.8, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221307494, Length=250, Percent_Identity=24.8, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757968, Length=255, Percent_Identity=25.0980392156863, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI49355787, Length=255, Percent_Identity=25.0980392156863, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37594469, Length=192, Percent_Identity=27.6041666666667, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI37594471, Length=191, Percent_Identity=26.7015706806283, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI116235487, Length=212, Percent_Identity=25.9433962264151, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=36.9047619047619, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI221142681, Length=269, Percent_Identity=33.8289962825279, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=32.9457364341085, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=30.6818181818182, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=31.6981132075472, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=25.7894736842105, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=242, Percent_Identity=44.2148760330579, Blast_Score=187, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=30.952380952381, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=31.2, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=236, Percent_Identity=26.6949152542373, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=28.4584980237154, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=23.6220472440945, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=267, Percent_Identity=26.2172284644195, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=213, Percent_Identity=28.6384976525822, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=255, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=215, Percent_Identity=24.6511627906977, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19922422, Length=252, Percent_Identity=36.1111111111111, Blast_Score=180, Evalue=7e-46,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=31.3253012048193, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=31.3253012048193, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=28.4584980237154, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI21357171, Length=252, Percent_Identity=26.5873015873016, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920382, Length=262, Percent_Identity=26.7175572519084, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654903, Length=200, Percent_Identity=27.5, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24650670, Length=253, Percent_Identity=27.6679841897233, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583165, Length=222, Percent_Identity=27.027027027027, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27706; Mature: 27575

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTPYVLRADDDGVTTLTLNRPQARNALSQGMLEALRAALAEIAADASVQVLIIAGSGP
CCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
AFCAGHDLKELRAGNYAPDYVDALFAACAEVMQAIVHLPKPVIARVHGVATAAGAQLVAS
CEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ADLAFAADDARFATPGVNIGLFCSTPMVALSRNLTRKHAMQMLLSGDLIDADTAFRFGLI
CCCEEECCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
NEVVPAAELEQRTLAFARQIASKSPLTLAIGKEAFYRQAELSLADAYAYTSQVMAKNLQA
HHHCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EDAKEGIDAFLGKRQPVWCGR
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TDTPYVLRADDDGVTTLTLNRPQARNALSQGMLEALRAALAEIAADASVQVLIIAGSGP
CCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
AFCAGHDLKELRAGNYAPDYVDALFAACAEVMQAIVHLPKPVIARVHGVATAAGAQLVAS
CEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ADLAFAADDARFATPGVNIGLFCSTPMVALSRNLTRKHAMQMLLSGDLIDADTAFRFGLI
CCCEEECCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
NEVVPAAELEQRTLAFARQIASKSPLTLAIGKEAFYRQAELSLADAYAYTSQVMAKNLQA
HHHCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EDAKEGIDAFLGKRQPVWCGR
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA