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The map label for this gene is btuB [H]

Identifier: 192290933

GI number: 192290933

Start: 2755981

End: 2758308

Strand: Reverse

Name: btuB [H]

Synonym: Rpal_2550

Alternate gene names: 192290933

Gene position: 2758308-2755981 (Counterclockwise)

Preceding gene: 192290937

Following gene: 192290927

Centisome position: 48.02

GC content: 65.29

Gene sequence:

>2328_bases
ATGATTCAACGGGCATTCACGCGCGAATCCGCACGGAGCCGACTTCTTGCTTCCACCTGCGGACTGAGCGTCGCGCTGGC
CTGCGTGATGACGGCAGGATCGAGCACCGCGACGCGGGCTCAGACTGCCGACTCGACGACACCGCGCGCGCGCCATGTGT
TGCCGGAAGTGCGCGTGGAATCGGCGAATCCACCGAAGCCGCAACGCGCGGCACGGTCGAGCGACGGCCGCGGTGCACGC
CGCGCCGCGGCGCAAGCGCCAAAGCCCGCGCCCGCTCAGCAGGCCGCAGCAGCGACGTCAGATAATTCGCTCAAGCCAAT
GGGGGGCAAGCTTCCGCAGCCCGGAATTCCGCACGCCGCGCAGCAGAGCATCACCGTGGTCGATCGTGCGCAGATCGAAC
AGACCAGCCCGACCAGCCTGCTCGATATCCTGCAGAACGTGCCGGGCGTGTCGATCGCGCGCGCCGGCGGCATCGGCGGT
CAGATCTATCTGCGCGGCTTCTCGTCGAACAACTGGCGCTCGCCGATGTATATCGACGGCGACCGTTTCCGCGGTCGCAA
CACGCTGCAGCTCAACTACTTCGCACCCGAAGAGATCGAGCGGGTCGAGGTGGTGCGCGGACCCGCCTCGGTGGTGTACG
GCTCCGAGGCGATGACCGGGCTCGTCAACGTCGTCACCCGCCGTCCCCAGGTCGATCCGTTCGGTCCGTGGCGCGTCACC
CATGGCGGGTTCTCGGCCGGCTACAGCTCGGTCGACAACGGCTTCAGCACTTATGAATGGGCGCAGGCGGCGGGCATGGG
CTTCGATGTGCTCGGCGGCGTCAGCTATCGCAAAGGCGGCAATTATCAAACGCCCAGCGGACCGGCGCTGAACAGCGATT
ACGAAAGCATCGGCGGCAATCTGCGGCTGGGCTGGTCACCGACCAGCAACCAGCGCTTTGAACTGACGCTGCGCGACTAT
TGGGAAGAAGACGGCCGCGCCGGTGGCGTCGGCGGCGCGCCGGGCTGGCCCTATATGCAGGTGCGGCAGCAGCCGAACCA
GGTGCACATGGCGAAGCTGTCCTATGCCGGCGACTTCACCGATCAGCTGGTCCAGCATGTCGAAGGCTCGTTCTACGCCA
ATTACTTCGACACCAAGCTCTCCACGGTGAACACCGCGACGCCGGGCGTCGTCACCTCCTCGGTCAGCCATGTCGGTGGC
CCATTGGTGATCGGCGGCCGGGTGCAAGGCGTGATTCCGTGGGATCCGGCGCCGTGGGGACTGCTCAAGACGACATTCGG
CTTCGACGGCTTCCAGGAATGGCGTCCCGGCAGCACGTCGTGGTCGGTGCGGCAGACCGCGACGAGTTCGACGATCACGC
CTGAGTTCAAGACCGGCCCGGACAACACCCAGGCCAATGCCGGCGCGTTCATGCTGCACGAGTGGAAGCCGATTGATCTG
CTGACGCTGTCGGCCGGCGGCCGGTTTGACTGGTTCAACACCACCTCGGAACTGTCGCCGGTGAGTTCGGCGAGAGCCCT
CGCGGTGCTTCGGCAGAACAGCGACGTCACCGCCACCGCCCCGACCGGCAGCATTGGCGCCACGGTTCACGTGACGCCGA
TCCTCGATCTGCTCGCCTCGGTGGGCACCTCGTATCGCTATCCGACCAATTCCGAGCTGTTCGCGTTCTCAGGCACATCG
ATCCCGAATCCGGAGCTGAAGCCGGAGACCGGTCTCACCTACGAGGGCGGCTTCCAGCTCAACTTCCCGACCGCCACCTT
CAAGGTGACGGCGTTCGACAGCCACTACGCGAATTTCCTGCAGTCGGTGTTCGTCAACTATAACGGGGCATCGGTGACGC
AGAGCCAGAACGTCGGCCATGCCACCGTGAACGGCGTCGAAGCCGAATGGCGCTGGCAGGCGACGTCGTCGTTCAACCTG
CACGGCAATGCCGCGTGGCTGCAGGCGACCAACACCGACACCGATGCGCCGCTGCCGTTCATTGCGCCGTGGAAGGGACG
TGTCGGCGTGCAATACGCGCCGATCGGCTCCGGCTACGCGGTCGGTGCCGCGGTGGATTGGGCATCGGCCAAGACCCGCA
TCAACACCACGCAGGAATACCCGACTGGTGCCTATGCGGTGCCGAGCATCTACGCCTCGTTCGATCTCGGCGTATTGGTC
TCGCGCCGACTCGGCGACACCAAGCTGCACCTCAGCCTGCAGAACATCTTCGACACCTCCTATGCGAGCGCGTCGACTTA
TGTGAACCGGTCGTACAGCCAGTCGATGTACAACCCGCTGCTGGAGCCAGGCCGCAATTTCACCGCCCGGCTCACGCACA
CCTTCTGA

Upstream 100 bases:

>100_bases
GGTGGTAATCGCGCGCAACGCTAACGTGGGGATGGTGGTGGTCGAGATGACGACTCGATAAGGACCCGTTCACTAATCGT
GAACTGGGGGACGCCACCAA

Downstream 100 bases:

>100_bases
CGACAAGCACGTCTGATGGGAGACGGACCGTCCGGCGGCGTTGGACGGTCCGCCTCCGCTGCGGGTCCTCAGGGGCATCG
GACCCACATTCGCCACAGCA

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 775; Mature: 775

Protein sequence:

>775_residues
MIQRAFTRESARSRLLASTCGLSVALACVMTAGSSTATRAQTADSTTPRARHVLPEVRVESANPPKPQRAARSSDGRGAR
RAAAQAPKPAPAQQAAAATSDNSLKPMGGKLPQPGIPHAAQQSITVVDRAQIEQTSPTSLLDILQNVPGVSIARAGGIGG
QIYLRGFSSNNWRSPMYIDGDRFRGRNTLQLNYFAPEEIERVEVVRGPASVVYGSEAMTGLVNVVTRRPQVDPFGPWRVT
HGGFSAGYSSVDNGFSTYEWAQAAGMGFDVLGGVSYRKGGNYQTPSGPALNSDYESIGGNLRLGWSPTSNQRFELTLRDY
WEEDGRAGGVGGAPGWPYMQVRQQPNQVHMAKLSYAGDFTDQLVQHVEGSFYANYFDTKLSTVNTATPGVVTSSVSHVGG
PLVIGGRVQGVIPWDPAPWGLLKTTFGFDGFQEWRPGSTSWSVRQTATSSTITPEFKTGPDNTQANAGAFMLHEWKPIDL
LTLSAGGRFDWFNTTSELSPVSSARALAVLRQNSDVTATAPTGSIGATVHVTPILDLLASVGTSYRYPTNSELFAFSGTS
IPNPELKPETGLTYEGGFQLNFPTATFKVTAFDSHYANFLQSVFVNYNGASVTQSQNVGHATVNGVEAEWRWQATSSFNL
HGNAAWLQATNTDTDAPLPFIAPWKGRVGVQYAPIGSGYAVGAAVDWASAKTRINTTQEYPTGAYAVPSIYASFDLGVLV
SRRLGDTKLHLSLQNIFDTSYASASTYVNRSYSQSMYNPLLEPGRNFTARLTHTF

Sequences:

>Translated_775_residues
MIQRAFTRESARSRLLASTCGLSVALACVMTAGSSTATRAQTADSTTPRARHVLPEVRVESANPPKPQRAARSSDGRGAR
RAAAQAPKPAPAQQAAAATSDNSLKPMGGKLPQPGIPHAAQQSITVVDRAQIEQTSPTSLLDILQNVPGVSIARAGGIGG
QIYLRGFSSNNWRSPMYIDGDRFRGRNTLQLNYFAPEEIERVEVVRGPASVVYGSEAMTGLVNVVTRRPQVDPFGPWRVT
HGGFSAGYSSVDNGFSTYEWAQAAGMGFDVLGGVSYRKGGNYQTPSGPALNSDYESIGGNLRLGWSPTSNQRFELTLRDY
WEEDGRAGGVGGAPGWPYMQVRQQPNQVHMAKLSYAGDFTDQLVQHVEGSFYANYFDTKLSTVNTATPGVVTSSVSHVGG
PLVIGGRVQGVIPWDPAPWGLLKTTFGFDGFQEWRPGSTSWSVRQTATSSTITPEFKTGPDNTQANAGAFMLHEWKPIDL
LTLSAGGRFDWFNTTSELSPVSSARALAVLRQNSDVTATAPTGSIGATVHVTPILDLLASVGTSYRYPTNSELFAFSGTS
IPNPELKPETGLTYEGGFQLNFPTATFKVTAFDSHYANFLQSVFVNYNGASVTQSQNVGHATVNGVEAEWRWQATSSFNL
HGNAAWLQATNTDTDAPLPFIAPWKGRVGVQYAPIGSGYAVGAAVDWASAKTRINTTQEYPTGAYAVPSIYASFDLGVLV
SRRLGDTKLHLSLQNIFDTSYASASTYVNRSYSQSMYNPLLEPGRNFTARLTHTF
>Mature_775_residues
MIQRAFTRESARSRLLASTCGLSVALACVMTAGSSTATRAQTADSTTPRARHVLPEVRVESANPPKPQRAARSSDGRGAR
RAAAQAPKPAPAQQAAAATSDNSLKPMGGKLPQPGIPHAAQQSITVVDRAQIEQTSPTSLLDILQNVPGVSIARAGGIGG
QIYLRGFSSNNWRSPMYIDGDRFRGRNTLQLNYFAPEEIERVEVVRGPASVVYGSEAMTGLVNVVTRRPQVDPFGPWRVT
HGGFSAGYSSVDNGFSTYEWAQAAGMGFDVLGGVSYRKGGNYQTPSGPALNSDYESIGGNLRLGWSPTSNQRFELTLRDY
WEEDGRAGGVGGAPGWPYMQVRQQPNQVHMAKLSYAGDFTDQLVQHVEGSFYANYFDTKLSTVNTATPGVVTSSVSHVGG
PLVIGGRVQGVIPWDPAPWGLLKTTFGFDGFQEWRPGSTSWSVRQTATSSTITPEFKTGPDNTQANAGAFMLHEWKPIDL
LTLSAGGRFDWFNTTSELSPVSSARALAVLRQNSDVTATAPTGSIGATVHVTPILDLLASVGTSYRYPTNSELFAFSGTS
IPNPELKPETGLTYEGGFQLNFPTATFKVTAFDSHYANFLQSVFVNYNGASVTQSQNVGHATVNGVEAEWRWQATSSFNL
HGNAAWLQATNTDTDAPLPFIAPWKGRVGVQYAPIGSGYAVGAAVDWASAKTRINTTQEYPTGAYAVPSIYASFDLGVLV
SRRLGDTKLHLSLQNIFDTSYASASTYVNRSYSQSMYNPLLEPGRNFTARLTHTF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=478, Percent_Identity=23.4309623430962, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI1790405, Length=140, Percent_Identity=35.7142857142857, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1786344, Length=151, Percent_Identity=31.1258278145695, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787024, Length=119, Percent_Identity=35.2941176470588, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 83345; Mature: 83345

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQRAFTRESARSRLLASTCGLSVALACVMTAGSSTATRAQTADSTTPRARHVLPEVRVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEEC
SANPPKPQRAARSSDGRGARRAAAQAPKPAPAQQAAAATSDNSLKPMGGKLPQPGIPHAA
CCCCCCHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
QQSITVVDRAQIEQTSPTSLLDILQNVPGVSIARAGGIGGQIYLRGFSSNNWRSPMYIDG
HCCEEEEEHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCCCCCEEECC
DRFRGRNTLQLNYFAPEEIERVEVVRGPASVVYGSEAMTGLVNVVTRRPQVDPFGPWRVT
CCCCCCCEEEEEECCHHHHHHHHEECCCCCEEECCHHHHHHHHHHHCCCCCCCCCCEEEE
HGGFSAGYSSVDNGFSTYEWAQAAGMGFDVLGGVSYRKGGNYQTPSGPALNSDYESIGGN
CCCCCCCCHHHCCCCCHHHHHHHCCCCCHHHCCCEECCCCCCCCCCCCCCCCCHHHCCCC
LRLGWSPTSNQRFELTLRDYWEEDGRAGGVGGAPGWPYMQVRQQPNQVHMAKLSYAGDFT
EEECCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCHH
DQLVQHVEGSFYANYFDTKLSTVNTATPGVVTSSVSHVGGPLVIGGRVQGVIPWDPAPWG
HHHHHHHCCCEEEEEHHCCCCEECCCCCCCEEHHHHHCCCCEEECCEEEEEECCCCCCCC
LLKTTFGFDGFQEWRPGSTSWSVRQTATSSTITPEFKTGPDNTQANAGAFMLHEWKPIDL
HHHHHCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEE
LTLSAGGRFDWFNTTSELSPVSSARALAVLRQNSDVTATAPTGSIGATVHVTPILDLLAS
EEECCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCEEEECCCCCCCCEEEHHHHHHHHHH
VGTSYRYPTNSELFAFSGTSIPNPELKPETGLTYEGGFQLNFPTATFKVTAFDSHYANFL
HCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEECCCEEEECCCEEEEEEECCHHHHHHH
QSVFVNYNGASVTQSQNVGHATVNGVEAEWRWQATSSFNLHGNAAWLQATNTDTDAPLPF
HHHHCCCCCCCEECCCCCCCEEECCCCCEEEEEECCEEEECCCEEEEEECCCCCCCCCCE
IAPWKGRVGVQYAPIGSGYAVGAAVDWASAKTRINTTQEYPTGAYAVPSIYASFDLGVLV
ECCCCCCCCEEEECCCCCEEEEEEEECCCCCEECCCCCCCCCCCEECCHHHHHCCHHHHH
SRRLGDTKLHLSLQNIFDTSYASASTYVNRSYSQSMYNPLLEPGRNFTARLTHTF
HHCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure
MIQRAFTRESARSRLLASTCGLSVALACVMTAGSSTATRAQTADSTTPRARHVLPEVRVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEEC
SANPPKPQRAARSSDGRGARRAAAQAPKPAPAQQAAAATSDNSLKPMGGKLPQPGIPHAA
CCCCCCHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
QQSITVVDRAQIEQTSPTSLLDILQNVPGVSIARAGGIGGQIYLRGFSSNNWRSPMYIDG
HCCEEEEEHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCCCCCEEECC
DRFRGRNTLQLNYFAPEEIERVEVVRGPASVVYGSEAMTGLVNVVTRRPQVDPFGPWRVT
CCCCCCCEEEEEECCHHHHHHHHEECCCCCEEECCHHHHHHHHHHHCCCCCCCCCCEEEE
HGGFSAGYSSVDNGFSTYEWAQAAGMGFDVLGGVSYRKGGNYQTPSGPALNSDYESIGGN
CCCCCCCCHHHCCCCCHHHHHHHCCCCCHHHCCCEECCCCCCCCCCCCCCCCCHHHCCCC
LRLGWSPTSNQRFELTLRDYWEEDGRAGGVGGAPGWPYMQVRQQPNQVHMAKLSYAGDFT
EEECCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCHH
DQLVQHVEGSFYANYFDTKLSTVNTATPGVVTSSVSHVGGPLVIGGRVQGVIPWDPAPWG
HHHHHHHCCCEEEEEHHCCCCEECCCCCCCEEHHHHHCCCCEEECCEEEEEECCCCCCCC
LLKTTFGFDGFQEWRPGSTSWSVRQTATSSTITPEFKTGPDNTQANAGAFMLHEWKPIDL
HHHHHCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEE
LTLSAGGRFDWFNTTSELSPVSSARALAVLRQNSDVTATAPTGSIGATVHVTPILDLLAS
EEECCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCEEEECCCCCCCCEEEHHHHHHHHHH
VGTSYRYPTNSELFAFSGTSIPNPELKPETGLTYEGGFQLNFPTATFKVTAFDSHYANFL
HCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEECCCEEEECCCEEEEEEECCHHHHHHH
QSVFVNYNGASVTQSQNVGHATVNGVEAEWRWQATSSFNLHGNAAWLQATNTDTDAPLPF
HHHHCCCCCCCEECCCCCCCEEECCCCCEEEEEECCEEEECCCEEEEEECCCCCCCCCCE
IAPWKGRVGVQYAPIGSGYAVGAAVDWASAKTRINTTQEYPTGAYAVPSIYASFDLGVLV
ECCCCCCCCEEEECCCCCEEEEEEEECCCCCEECCCCCCCCCCCEECCHHHHHCCHHHHH
SRRLGDTKLHLSLQNIFDTSYASASTYVNRSYSQSMYNPLLEPGRNFTARLTHTF
HHCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA