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The map label for this gene is fixL [H]

Identifier: 192290918

GI number: 192290918

Start: 2738804

End: 2740222

Strand: Reverse

Name: fixL [H]

Synonym: Rpal_2535

Alternate gene names: 192290918

Gene position: 2740222-2738804 (Counterclockwise)

Preceding gene: 192290919

Following gene: 192290914

Centisome position: 47.71

GC content: 66.88

Gene sequence:

>1419_bases
ATGACTCGAAAATCGTTATCAAGAACATTTCTCGCGCCGTGGCTGCTCGGCGCGACCTTGCTGTTGTTCATCGTCATCGC
CGGCGCGCTGGTGCTCAACCTGGTGCGGCTCCGCGACAGCTTTGCCTGGGTGCAACAAACCAACAAGGCGTTGCTGGCGA
TCTCCGCGATCCAGCAGGCCGTGCTCGAAGCCGAGACCAGCGAGCGCGGCTTTCTGCTGACAGGTATCGAAACCTATCGT
GACAGTTACATCCGCGCCCGCGACGCGCTTGCCGCCCGGCTCGACGGGCTGCGCGCGGTGCTCGCCGACAACCCGGAGCA
GATCGCCCATATCGATGAGCTCAGGCTACTGACCGACATGCGCATGGCGCAGCTCGGCCGCGTCGTCGAACTCGGCCCAG
AGCGGATGCGCGAGGCGCTTGATATTCTCGAGCAGGCCCGTGTCGACCGCCTGACCGAGCGGATCGAGACCAGCCTGTCG
GTGCTGACCCGTGCCGAACAGGCGCTGCTGATCCAGCGGCTGTCGAAGCACGATCGCGAGAGCCTGGCCGCGGCGCTGAT
CACTGCGTGCCTCTTGATCCTCGCGGTCGGCAGCGCAGCGATCGCCGCATTCCTGCTCGAGCATCAGCGCGCAGTGACAC
GGCAGCAGGAGGCCGACCAGCGGCTGCAGGCGCTGCAGGCCGAATTGCTGCGGGTCGCCCGCCTCAGCACCATGGGCGAG
ATGTCGAGCGCACTGGCCCACGAACTCAACCAGCCGCTCGCCGCCGTCACCAACTACGTGCAAGGTTCGCGGCGACTGAT
CGAAGCCAGCAGCCATCCCGACAAGAGCAAAATCAGCACCGCGCTCGACAAGGCCGCCCAGCAGACGCTGCGCGCCGGCG
CGGTGATCCAGCGGCTGCGCGAATTCGTCGGCCGCGGCGAGACCGACAAGACCATCGAGAGCCTGCGCGCGATCGCCGAA
GATGCCCTCGCCCTCGCCTCGGTGCTGACCCGCGACCGCCCGGTCGACGTCGCGCTGTCGCTCGATCCCGCGTTCGACCG
GGTGCTGGTCGACAAGGTGCAGGTGCAGCAGGTGTTCCTCAATTTGATCCGCAACGCGTTCGAAGCGATGCGCGATCAGC
GCGAGCGCAAGCTGACGATCGGCAGCCGGCTGGTCGAAGACGACATGGTGGAAGTGGTGGTCGCCGACAGCGGCCCGGGT
CTCGACGCGCTGATCGCCGAGCGGATCTTCCAGCCGTTCGCGACCACCAAGGCGGACGGCATGGGCATCGGGCTGTCGAT
CTCGCAGACCATCATCCAGGCGCATGGCGGCTCGATCAAGGCAGAGCCGGCGCCGGGTGGCGGCACGCTGTTCCGCTTCA
CCCTGCCCTGCTCCGATCCGATCCGCCAGCACCTCTCCTTCAGCCCCGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
CACCAGAACTGCGTCCCTAGACCAAATCTACAGAGCTGATGCGATTCAATCCGCTCCGGATTTGCTTTAGGGTATCCCGG
TCGTGATTGCGTGAGTGCCG

Downstream 100 bases:

>100_bases
TCGGCGTTTCGATGCTAACCGCCGGTGTGGTCCAGCACCGGCCGAGGCGCGTCGGCCAGCGTTGCAACGCGCCGCGCGAA
GTGATCGTACAACCCGGCGA

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTRKSLSRTFLAPWLLGATLLLFIVIAGALVLNLVRLRDSFAWVQQTNKALLAISAIQQAVLEAETSERGFLLTGIETYR
DSYIRARDALAARLDGLRAVLADNPEQIAHIDELRLLTDMRMAQLGRVVELGPERMREALDILEQARVDRLTERIETSLS
VLTRAEQALLIQRLSKHDRESLAAALITACLLILAVGSAAIAAFLLEHQRAVTRQQEADQRLQALQAELLRVARLSTMGE
MSSALAHELNQPLAAVTNYVQGSRRLIEASSHPDKSKISTALDKAAQQTLRAGAVIQRLREFVGRGETDKTIESLRAIAE
DALALASVLTRDRPVDVALSLDPAFDRVLVDKVQVQQVFLNLIRNAFEAMRDQRERKLTIGSRLVEDDMVEVVVADSGPG
LDALIAERIFQPFATTKADGMGIGLSISQTIIQAHGGSIKAEPAPGGGTLFRFTLPCSDPIRQHLSFSPAAE

Sequences:

>Translated_472_residues
MTRKSLSRTFLAPWLLGATLLLFIVIAGALVLNLVRLRDSFAWVQQTNKALLAISAIQQAVLEAETSERGFLLTGIETYR
DSYIRARDALAARLDGLRAVLADNPEQIAHIDELRLLTDMRMAQLGRVVELGPERMREALDILEQARVDRLTERIETSLS
VLTRAEQALLIQRLSKHDRESLAAALITACLLILAVGSAAIAAFLLEHQRAVTRQQEADQRLQALQAELLRVARLSTMGE
MSSALAHELNQPLAAVTNYVQGSRRLIEASSHPDKSKISTALDKAAQQTLRAGAVIQRLREFVGRGETDKTIESLRAIAE
DALALASVLTRDRPVDVALSLDPAFDRVLVDKVQVQQVFLNLIRNAFEAMRDQRERKLTIGSRLVEDDMVEVVVADSGPG
LDALIAERIFQPFATTKADGMGIGLSISQTIIQAHGGSIKAEPAPGGGTLFRFTLPCSDPIRQHLSFSPAAE
>Mature_471_residues
TRKSLSRTFLAPWLLGATLLLFIVIAGALVLNLVRLRDSFAWVQQTNKALLAISAIQQAVLEAETSERGFLLTGIETYRD
SYIRARDALAARLDGLRAVLADNPEQIAHIDELRLLTDMRMAQLGRVVELGPERMREALDILEQARVDRLTERIETSLSV
LTRAEQALLIQRLSKHDRESLAAALITACLLILAVGSAAIAAFLLEHQRAVTRQQEADQRLQALQAELLRVARLSTMGEM
SSALAHELNQPLAAVTNYVQGSRRLIEASSHPDKSKISTALDKAAQQTLRAGAVIQRLREFVGRGETDKTIESLRAIAED
ALALASVLTRDRPVDVALSLDPAFDRVLVDKVQVQQVFLNLIRNAFEAMRDQRERKLTIGSRLVEDDMVEVVVADSGPGL
DALIAERIFQPFATTKADGMGIGLSISQTIIQAHGGSIKAEPAPGGGTLFRFTLPCSDPIRQHLSFSPAAE

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=233, Percent_Identity=32.618025751073, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=26.6949152542373, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790300, Length=235, Percent_Identity=28.0851063829787, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=124, Percent_Identity=35.4838709677419, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=152, Percent_Identity=33.5526315789474, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51720; Mature: 51589

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKSLSRTFLAPWLLGATLLLFIVIAGALVLNLVRLRDSFAWVQQTNKALLAISAIQQA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLEAETSERGFLLTGIETYRDSYIRARDALAARLDGLRAVLADNPEQIAHIDELRLLTDM
HHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RMAQLGRVVELGPERMREALDILEQARVDRLTERIETSLSVLTRAEQALLIQRLSKHDRE
HHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAAALITACLLILAVGSAAIAAFLLEHQRAVTRQQEADQRLQALQAELLRVARLSTMGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSSALAHELNQPLAAVTNYVQGSRRLIEASSHPDKSKISTALDKAAQQTLRAGAVIQRLR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EFVGRGETDKTIESLRAIAEDALALASVLTRDRPVDVALSLDPAFDRVLVDKVQVQQVFL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
NLIRNAFEAMRDQRERKLTIGSRLVEDDMVEVVVADSGPGLDALIAERIFQPFATTKADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
MGIGLSISQTIIQAHGGSIKAEPAPGGGTLFRFTLPCSDPIRQHLSFSPAAE
CCCCHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
TRKSLSRTFLAPWLLGATLLLFIVIAGALVLNLVRLRDSFAWVQQTNKALLAISAIQQA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLEAETSERGFLLTGIETYRDSYIRARDALAARLDGLRAVLADNPEQIAHIDELRLLTDM
HHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RMAQLGRVVELGPERMREALDILEQARVDRLTERIETSLSVLTRAEQALLIQRLSKHDRE
HHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAAALITACLLILAVGSAAIAAFLLEHQRAVTRQQEADQRLQALQAELLRVARLSTMGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSSALAHELNQPLAAVTNYVQGSRRLIEASSHPDKSKISTALDKAAQQTLRAGAVIQRLR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EFVGRGETDKTIESLRAIAEDALALASVLTRDRPVDVALSLDPAFDRVLVDKVQVQQVFL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
NLIRNAFEAMRDQRERKLTIGSRLVEDDMVEVVVADSGPGLDALIAERIFQPFATTKADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
MGIGLSISQTIIQAHGGSIKAEPAPGGGTLFRFTLPCSDPIRQHLSFSPAAE
CCCCHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]