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The map label for this gene is gltS [H]

Identifier: 192290887

GI number: 192290887

Start: 2703357

End: 2704553

Strand: Reverse

Name: gltS [H]

Synonym: Rpal_2504

Alternate gene names: 192290887

Gene position: 2704553-2703357 (Counterclockwise)

Preceding gene: 192290888

Following gene: 192290886

Centisome position: 47.08

GC content: 61.82

Gene sequence:

>1197_bases
ATGGAAATCGATGCCTTCTATACGTTCACGATCGCCGTCATCCTGCTTCTGACCGGCAAGATAGCGACACTGAACGCGCC
GCTGCTGCGCAAATACTCCATTCCCGAGCCGGTCATCGGCGGGTTCCTGTGCACAGCGGTGGTCGCCCTCAGCTACACGC
TTTTTGATCGGCGGCTCACCTTCGATCTCGCGATGCGCGACTTTCTGCTTCTCTTGTTCTTTGCGGGCATCGGCCTGAAG
GCCGAGATCAAGATGCTTCTGGCCGGCGGCCGTCCGCTGGTCATCCTGCTGTCGCTCGCTGACGTCCTGATGCTGATCCA
GAACTTTGCCGGCATGGGGATGGCTTCGCTGTTCGGACTGGAAGCCAAGGCCGGACTGATGGTGGGCTCGATCTCGCTGA
CCGGCGGCATCGGCACGACGCTCGCCTGGGCACCGACATTCACCGAGCGGCTCGGCATCACCAACGCAATGGAATTGGGT
GTTGCGGCCAATACGGTCGGGCTGATAGCGGCCTGCATGATCGGCGGCCCCGTGGCCGCCTTCCTGATGAAGCGTCACGG
TGTCGCCGCCACCGACGACCCCGAACTCGCCATCGGCGTTTCCAATGACGGCCGTCAGCCCAAGCTCGACTACTTCTGCA
TTCTTTGGGCTGTGCTGGCTTTGAACCTCGCCATCATCCTCGGGCTCGGCATTCATGATGCGCTGACACGCACAGGCCTG
AACCTGCCCGCCTTCGTAAGCTGCCTGATGGCCGGAATCCTGATCCGCAACCTCATGCCCATCGCTGTGAGTTCGCGCGT
CCAGAAGCTTTGGCCCGGCGTGGACGACGGGCTGACCCTGGTATCCGATCTCGCCCTCGGCCTGTTCCTGACCATGGCGT
TGATGGGGCTTCAGCTCTGGCAACTCGATGGCGTCTTCACCTTCGTCGCCTCGGCCCTTTTCGTGCAGATCATCCTGGCC
GTCATCTTCACCATATCGATCGTGTTCCGCTTCATGGGGAAGGACTACGAGGCAGTGGTGATCTCGGCGGGCTTCGGCGG
AATCGCGCTGGGCTCGACGGCCACCGCCATTGCCAACATGACAGCGGTCACCCAGCAATACGGCGCCGCCCACCGCGCCT
TCATCGTCGTGCCGCTGGTGTGCGGCTTCTTCATCGACATCGTCAACGCCCTGATCATCTCCGCCTTTGTCGGTTGA

Upstream 100 bases:

>100_bases
CCACCACGAGCGTTGAGCCAGCCGCTCAATTGAAACCCTGTCCGCCGTGATCGCAAAACTGCTGTCACTGCCTGGTCCCC
CATTGGAATCCGAAAAGCCT

Downstream 100 bases:

>100_bases
GAGAGCGTGGTATTATGGTTCGGGCATCCTATGGGAGAAATCCGAACTACAGCGCTCGGCGCCGGGCCATCTTTAAGAAC
GTGCCCTGACGGTGCAACCA

Product: sodium/glutamate symporter

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MEIDAFYTFTIAVILLLTGKIATLNAPLLRKYSIPEPVIGGFLCTAVVALSYTLFDRRLTFDLAMRDFLLLLFFAGIGLK
AEIKMLLAGGRPLVILLSLADVLMLIQNFAGMGMASLFGLEAKAGLMVGSISLTGGIGTTLAWAPTFTERLGITNAMELG
VAANTVGLIAACMIGGPVAAFLMKRHGVAATDDPELAIGVSNDGRQPKLDYFCILWAVLALNLAIILGLGIHDALTRTGL
NLPAFVSCLMAGILIRNLMPIAVSSRVQKLWPGVDDGLTLVSDLALGLFLTMALMGLQLWQLDGVFTFVASALFVQIILA
VIFTISIVFRFMGKDYEAVVISAGFGGIALGSTATAIANMTAVTQQYGAAHRAFIVVPLVCGFFIDIVNALIISAFVG

Sequences:

>Translated_398_residues
MEIDAFYTFTIAVILLLTGKIATLNAPLLRKYSIPEPVIGGFLCTAVVALSYTLFDRRLTFDLAMRDFLLLLFFAGIGLK
AEIKMLLAGGRPLVILLSLADVLMLIQNFAGMGMASLFGLEAKAGLMVGSISLTGGIGTTLAWAPTFTERLGITNAMELG
VAANTVGLIAACMIGGPVAAFLMKRHGVAATDDPELAIGVSNDGRQPKLDYFCILWAVLALNLAIILGLGIHDALTRTGL
NLPAFVSCLMAGILIRNLMPIAVSSRVQKLWPGVDDGLTLVSDLALGLFLTMALMGLQLWQLDGVFTFVASALFVQIILA
VIFTISIVFRFMGKDYEAVVISAGFGGIALGSTATAIANMTAVTQQYGAAHRAFIVVPLVCGFFIDIVNALIISAFVG
>Mature_398_residues
MEIDAFYTFTIAVILLLTGKIATLNAPLLRKYSIPEPVIGGFLCTAVVALSYTLFDRRLTFDLAMRDFLLLLFFAGIGLK
AEIKMLLAGGRPLVILLSLADVLMLIQNFAGMGMASLFGLEAKAGLMVGSISLTGGIGTTLAWAPTFTERLGITNAMELG
VAANTVGLIAACMIGGPVAAFLMKRHGVAATDDPELAIGVSNDGRQPKLDYFCILWAVLALNLAIILGLGIHDALTRTGL
NLPAFVSCLMAGILIRNLMPIAVSSRVQKLWPGVDDGLTLVSDLALGLFLTMALMGLQLWQLDGVFTFVASALFVQIILA
VIFTISIVFRFMGKDYEAVVISAGFGGIALGSTATAIANMTAVTQQYGAAHRAFIVVPLVCGFFIDIVNALIISAFVG

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=381, Percent_Identity=39.1076115485564, Blast_Score=230, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 42089; Mature: 42089

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIDAFYTFTIAVILLLTGKIATLNAPLLRKYSIPEPVIGGFLCTAVVALSYTLFDRRLT
CCCCHHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FDLAMRDFLLLLFFAGIGLKAEIKMLLAGGRPLVILLSLADVLMLIQNFAGMGMASLFGL
HHHHHHHHHHHHHHHCCCCHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
EAKAGLMVGSISLTGGIGTTLAWAPTFTERLGITNAMELGVAANTVGLIAACMIGGPVAA
CHHCCEEEEEEEECCCCCCHHEECCHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHH
FLMKRHGVAATDDPELAIGVSNDGRQPKLDYFCILWAVLALNLAIILGLGIHDALTRTGL
HHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
NLPAFVSCLMAGILIRNLMPIAVSSRVQKLWPGVDDGLTLVSDLALGLFLTMALMGLQLW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QLDGVFTFVASALFVQIILAVIFTISIVFRFMGKDYEAVVISAGFGGIALGSTATAIANM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCHHHHHHHH
TAVTQQYGAAHRAFIVVPLVCGFFIDIVNALIISAFVG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIDAFYTFTIAVILLLTGKIATLNAPLLRKYSIPEPVIGGFLCTAVVALSYTLFDRRLT
CCCCHHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FDLAMRDFLLLLFFAGIGLKAEIKMLLAGGRPLVILLSLADVLMLIQNFAGMGMASLFGL
HHHHHHHHHHHHHHHCCCCHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
EAKAGLMVGSISLTGGIGTTLAWAPTFTERLGITNAMELGVAANTVGLIAACMIGGPVAA
CHHCCEEEEEEEECCCCCCHHEECCHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHH
FLMKRHGVAATDDPELAIGVSNDGRQPKLDYFCILWAVLALNLAIILGLGIHDALTRTGL
HHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
NLPAFVSCLMAGILIRNLMPIAVSSRVQKLWPGVDDGLTLVSDLALGLFLTMALMGLQLW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QLDGVFTFVASALFVQIILAVIFTISIVFRFMGKDYEAVVISAGFGGIALGSTATAIANM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCHHHHHHHH
TAVTQQYGAAHRAFIVVPLVCGFFIDIVNALIISAFVG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]