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The map label for this gene is bioB [H]

Identifier: 192290648

GI number: 192290648

Start: 2449362

End: 2450372

Strand: Reverse

Name: bioB [H]

Synonym: Rpal_2257

Alternate gene names: 192290648

Gene position: 2450372-2449362 (Counterclockwise)

Preceding gene: 192290655

Following gene: 192290643

Centisome position: 42.66

GC content: 66.07

Gene sequence:

>1011_bases
TTGAACTCGATCGCCCATTCCACGCTTGCCGCCGCCAGCCCCACCATCCGCCACGACTGGACCCGCGAGGAAGCCGCGGC
GATCTATCACGCGCCGTTCGCCGATCTGATGTTCCGGGCGCAGACCATCCACCGCCAGACCTTCGATCCGAACCAGGTCC
AGTGCAATCAGCTGCTCAACGTCAAGACCGGCGGCTGCGCTGAGGATTGCGGCTATTGCAGCCAGTCCGCCCATCACGAC
ACCGCCCTGCCCGCCTCCAAGCTGATGGAACCGGCCAAGGTGATCGAAGCCGCCAAGGCGGCCCGCGACGCCGGCGCGAC
GCGCTATTGCATGGGCGCGGCGTGGCGCTCGCCGAAGGAGCGCGACATGGCTCCGGTGATCGAGATGGTGAAGGGCGTCA
AAGCGCTCGGCATGGAAGCCTGCATGACGCTCGGCATGCTGACCGATGATCAGGCCAAGCAGCTCGCCGACGCCGGCCTC
GACTACTACAACCACAACATCGATACCTCGGAAGAGTTTTACGCCTCGGTGGTGAAGAGCCGCAGCTTCGGCGACCGCCT
CGACACGCTGGAGAAGGTGCAGGACGCCGGCATCAAGGTGTGCTGCGGCGGCATCCTCGGCCTCGGCGAGAAGCCGACCG
ACCGCGTCGAGATGCTGCGCACCCTCGCCAACCTGCCGCAGCATCCGGAAAGCGTGCCGATCAACATGCTGATCCCGATC
GAAGGCACCCCGATTGCCAAGACCGCAACCCCGGTCGATCCGTTCGAATTCGTCCGCACCATCGCGCTGGCGCGCATCAT
GATGCCGAAATCAGACGTCCGCCTCGCCGCCGGCCGCACCGCGATGAGTGACGAAATGCAGGCGCTGTGCTTCCTCGCCG
GCGCCAACTCGATCTTCATCGGCGACACCCTGCTGACCACCCCGAACCCGGGCGATAGCAAGGACCGGGCGCTGTTCAAC
CGCCTCGGCATCACCCCGCGCGACGACCTCGGCGTCCACGCGCACAGCTGA

Upstream 100 bases:

>100_bases
ACCATGGTCGGGCTGGCGTTGCATCGCCATGGGTTTCTTCACCCGCCGAAACCGCTTATGGCTGTGCACCAGCCGAACGA
CCCCGACCGGAGCACCGACG

Downstream 100 bases:

>100_bases
GACGCGAACCAAAAGACCGTCATGGCCGGGTTCATCCCGGCCATGACGAGCCGGCAAGTTATCAGTCCTCGCCGCGCAGC
AGCATCGTCAGGAAGGCGGT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MNSIAHSTLAAASPTIRHDWTREEAAAIYHAPFADLMFRAQTIHRQTFDPNQVQCNQLLNVKTGGCAEDCGYCSQSAHHD
TALPASKLMEPAKVIEAAKAARDAGATRYCMGAAWRSPKERDMAPVIEMVKGVKALGMEACMTLGMLTDDQAKQLADAGL
DYYNHNIDTSEEFYASVVKSRSFGDRLDTLEKVQDAGIKVCCGGILGLGEKPTDRVEMLRTLANLPQHPESVPINMLIPI
EGTPIAKTATPVDPFEFVRTIALARIMMPKSDVRLAAGRTAMSDEMQALCFLAGANSIFIGDTLLTTPNPGDSKDRALFN
RLGITPRDDLGVHAHS

Sequences:

>Translated_336_residues
MNSIAHSTLAAASPTIRHDWTREEAAAIYHAPFADLMFRAQTIHRQTFDPNQVQCNQLLNVKTGGCAEDCGYCSQSAHHD
TALPASKLMEPAKVIEAAKAARDAGATRYCMGAAWRSPKERDMAPVIEMVKGVKALGMEACMTLGMLTDDQAKQLADAGL
DYYNHNIDTSEEFYASVVKSRSFGDRLDTLEKVQDAGIKVCCGGILGLGEKPTDRVEMLRTLANLPQHPESVPINMLIPI
EGTPIAKTATPVDPFEFVRTIALARIMMPKSDVRLAAGRTAMSDEMQALCFLAGANSIFIGDTLLTTPNPGDSKDRALFN
RLGITPRDDLGVHAHS
>Mature_336_residues
MNSIAHSTLAAASPTIRHDWTREEAAAIYHAPFADLMFRAQTIHRQTFDPNQVQCNQLLNVKTGGCAEDCGYCSQSAHHD
TALPASKLMEPAKVIEAAKAARDAGATRYCMGAAWRSPKERDMAPVIEMVKGVKALGMEACMTLGMLTDDQAKQLADAGL
DYYNHNIDTSEEFYASVVKSRSFGDRLDTLEKVQDAGIKVCCGGILGLGEKPTDRVEMLRTLANLPQHPESVPINMLIPI
EGTPIAKTATPVDPFEFVRTIALARIMMPKSDVRLAAGRTAMSDEMQALCFLAGANSIFIGDTLLTTPNPGDSKDRALFN
RLGITPRDDLGVHAHS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=311, Percent_Identity=58.5209003215434, Blast_Score=400, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=48.7341772151899, Blast_Score=326, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36446; Mature: 36446

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIAHSTLAAASPTIRHDWTREEAAAIYHAPFADLMFRAQTIHRQTFDPNQVQCNQLLN
CCCHHHHHHHHCCCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHC
VKTGGCAEDCGYCSQSAHHDTALPASKLMEPAKVIEAAKAARDAGATRYCMGAAWRSPKE
CCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
RDMAPVIEMVKGVKALGMEACMTLGMLTDDQAKQLADAGLDYYNHNIDTSEEFYASVVKS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RSFGDRLDTLEKVQDAGIKVCCGGILGLGEKPTDRVEMLRTLANLPQHPESVPINMLIPI
CCCHHHHHHHHHHHHCCCEEEECHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEC
EGTPIAKTATPVDPFEFVRTIALARIMMPKSDVRLAAGRTAMSDEMQALCFLAGANSIFI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCEEEE
GDTLLTTPNPGDSKDRALFNRLGITPRDDLGVHAHS
CCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSIAHSTLAAASPTIRHDWTREEAAAIYHAPFADLMFRAQTIHRQTFDPNQVQCNQLLN
CCCHHHHHHHHCCCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHC
VKTGGCAEDCGYCSQSAHHDTALPASKLMEPAKVIEAAKAARDAGATRYCMGAAWRSPKE
CCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
RDMAPVIEMVKGVKALGMEACMTLGMLTDDQAKQLADAGLDYYNHNIDTSEEFYASVVKS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RSFGDRLDTLEKVQDAGIKVCCGGILGLGEKPTDRVEMLRTLANLPQHPESVPINMLIPI
CCCHHHHHHHHHHHHCCCEEEECHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEC
EGTPIAKTATPVDPFEFVRTIALARIMMPKSDVRLAAGRTAMSDEMQALCFLAGANSIFI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCEEEE
GDTLLTTPNPGDSKDRALFNRLGITPRDDLGVHAHS
CCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA