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The map label for this gene is opuCB [H]

Identifier: 192290642

GI number: 192290642

Start: 2443225

End: 2444772

Strand: Reverse

Name: opuCB [H]

Synonym: Rpal_2251

Alternate gene names: 192290642

Gene position: 2444772-2443225 (Counterclockwise)

Preceding gene: 192290643

Following gene: 192290635

Centisome position: 42.56

GC content: 65.37

Gene sequence:

>1548_bases
ATGAGCCTGTTCGCCGATCCGCGCTGGCGCGACGCGCTCGCCAACCTGCCCGACTATCTCGGCGCCCATGTTCGCGTCAG
CCTCGCGGCGCTCGCGCTCGGACTGATCGTCAGCCTGCCGCTGGCAATCTTCGCCCGGCATCGCCCAGTGCTCCGCGGCA
CACTGCTCGGCTTCGCCGGCATCGTCCAGACGATCCCAGGTCTGGCGTTGCTGGCGCTATTCTATCCGCTGCTGCTGGCG
CTGGCGGCATTGTCGATGAGCGCATTCGGCGTGAGCTTCTCTGCCTTCGGCTTCCTGCCCGCCGTGCTGGCGCTGGCGCT
GTATTCGATGCTGCCGGTACTGCGCAACACCATCACCGGGCTCGACGGTATTGATCCGGCGCTGATCGAAGCCGCCGAGG
GCGTCGGCATGACTGAACGCCAAGTCCTCACCATGGTCGAGCTGCCGCTGGCAATGCCGGTGATCATGGCCGGCATCCGC
ACGGCGGCGGTTTGGGTGATCGGCACCGCGACGCTGTCGACGCCGATCGGTCAGACCAGTCTCGGCAACTACATATTTGC
CGGCCTGCAGACTCAGAATTGGATTCTGGTGCTGTTCGGCTGTGCCGCCGCTGCCCTGCTGGCGCTCGCCGTCGATCAAC
TGCTGGCGCTGGTGGAGATCGGCCTGCGCCGCGGCAACCGTTGGCGGATCGGCTTAGGCACCGCGGGCATCGCCGCGATC
GTGATCGCGACACTGCTGCCGGGCGCCACCACTCGCACTTCTTACGTGATCGGCGCCAAAACCTTCACCGAGCAATACGT
ACTCGCCGCATTGCTGCAGCAGCGCCTCGAGTCTAGCGGTCTTGCGGCCACCACGCGTGCCGGCCTCGGCTCCAGCGTGA
TCTATGAAGCGCTCAGAAGCGGCGACATCGACGCCTATGTGGATTACTCCGGCACGCTATGGGCTAGCCAATTCAAACAC
TCCGGCATCGCCACGCGCGATCAGGTGCTCGCCGACCTGAAACAGGATCTCGCCAAAGACGGCGTCACCTTGCTCGGCGA
ACTCGGCTTCGCCAACGCCTATGCGCTGGTGATGCCGCGCGGCAAAGCCGAGGCGCTGAAGATCCGTTCGATCGACGACC
TCGCCACCCGCGCACCGTCGATGACGATCGCCGGCGACTACGAGTTCTTCTCCCGGCCGGAATGGGCCAACCTGCGCAGC
GCCTATGGGTTGGCGTTCAAGACCGAACGGCAGATGCAGCCGGACTTCATGTACGCCGCCGTCGCCGCCGGCGAAGTCGA
TCTCATCGCCGGCTACACCTCGGACGGGCTGATCGCGAAATACGATCTGGTGGTGCTCGACGATCCGAAACAGACGATCC
CGCCCTACGACGCGGTGCTGCTGCTCGCGCCGAAACGCGCGAGTGACAGCAAGCTGCGCACAGCCTTGAATCCCCTGCTC
GGCCGCATCGACATCGAGGCAATGCGCGAAGCCAATCTGCAAGCGGGATCAGGGGGCACACCGGACGCGGTAGCGCGCGC
GTTGTGGGAGCGTGTGAAGCCGCGGTAA

Upstream 100 bases:

>100_bases
AGCTTGCGGGCAACCGAGACGCCTACGTCGCCGACCTGCTGCGGACACCACGTCGGCAGGCGCAGCGGCTCGGCGCCTTG
CTTTCGCGAGGCGGCGCGGC

Downstream 100 bases:

>100_bases
CGCGGAGCGGATAAGCGGAACACCATCCGCTCCCAGACTCGCCCTTGCAAGCTGCTTGTACCGCTCTCGTCACTCAGAAA
GCCGCAGACGCCAACTCATC

Product: glycine/betaine ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSLFADPRWRDALANLPDYLGAHVRVSLAALALGLIVSLPLAIFARHRPVLRGTLLGFAGIVQTIPGLALLALFYPLLLA
LAALSMSAFGVSFSAFGFLPAVLALALYSMLPVLRNTITGLDGIDPALIEAAEGVGMTERQVLTMVELPLAMPVIMAGIR
TAAVWVIGTATLSTPIGQTSLGNYIFAGLQTQNWILVLFGCAAAALLALAVDQLLALVEIGLRRGNRWRIGLGTAGIAAI
VIATLLPGATTRTSYVIGAKTFTEQYVLAALLQQRLESSGLAATTRAGLGSSVIYEALRSGDIDAYVDYSGTLWASQFKH
SGIATRDQVLADLKQDLAKDGVTLLGELGFANAYALVMPRGKAEALKIRSIDDLATRAPSMTIAGDYEFFSRPEWANLRS
AYGLAFKTERQMQPDFMYAAVAAGEVDLIAGYTSDGLIAKYDLVVLDDPKQTIPPYDAVLLLAPKRASDSKLRTALNPLL
GRIDIEAMREANLQAGSGGTPDAVARALWERVKPR

Sequences:

>Translated_515_residues
MSLFADPRWRDALANLPDYLGAHVRVSLAALALGLIVSLPLAIFARHRPVLRGTLLGFAGIVQTIPGLALLALFYPLLLA
LAALSMSAFGVSFSAFGFLPAVLALALYSMLPVLRNTITGLDGIDPALIEAAEGVGMTERQVLTMVELPLAMPVIMAGIR
TAAVWVIGTATLSTPIGQTSLGNYIFAGLQTQNWILVLFGCAAAALLALAVDQLLALVEIGLRRGNRWRIGLGTAGIAAI
VIATLLPGATTRTSYVIGAKTFTEQYVLAALLQQRLESSGLAATTRAGLGSSVIYEALRSGDIDAYVDYSGTLWASQFKH
SGIATRDQVLADLKQDLAKDGVTLLGELGFANAYALVMPRGKAEALKIRSIDDLATRAPSMTIAGDYEFFSRPEWANLRS
AYGLAFKTERQMQPDFMYAAVAAGEVDLIAGYTSDGLIAKYDLVVLDDPKQTIPPYDAVLLLAPKRASDSKLRTALNPLL
GRIDIEAMREANLQAGSGGTPDAVARALWERVKPR
>Mature_514_residues
SLFADPRWRDALANLPDYLGAHVRVSLAALALGLIVSLPLAIFARHRPVLRGTLLGFAGIVQTIPGLALLALFYPLLLAL
AALSMSAFGVSFSAFGFLPAVLALALYSMLPVLRNTITGLDGIDPALIEAAEGVGMTERQVLTMVELPLAMPVIMAGIRT
AAVWVIGTATLSTPIGQTSLGNYIFAGLQTQNWILVLFGCAAAALLALAVDQLLALVEIGLRRGNRWRIGLGTAGIAAIV
IATLLPGATTRTSYVIGAKTFTEQYVLAALLQQRLESSGLAATTRAGLGSSVIYEALRSGDIDAYVDYSGTLWASQFKHS
GIATRDQVLADLKQDLAKDGVTLLGELGFANAYALVMPRGKAEALKIRSIDDLATRAPSMTIAGDYEFFSRPEWANLRSA
YGLAFKTERQMQPDFMYAAVAAGEVDLIAGYTSDGLIAKYDLVVLDDPKQTIPPYDAVLLLAPKRASDSKLRTALNPLLG
RIDIEAMREANLQAGSGGTPDAVARALWERVKPR

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1732

COG function: function code M; Periplasmic glycine betaine/choline-binding (lipo)protein of an ABC-type transport system (osmoprotectant binding protein)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788449, Length=171, Percent_Identity=38.5964912280702, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788451, Length=205, Percent_Identity=32.1951219512195, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789033, Length=194, Percent_Identity=28.3505154639175, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 54801; Mature: 54669

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFADPRWRDALANLPDYLGAHVRVSLAALALGLIVSLPLAIFARHRPVLRGTLLGFAG
CCCCCCCCHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IVQTIPGLALLALFYPLLLALAALSMSAFGVSFSAFGFLPAVLALALYSMLPVLRNTITG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDGIDPALIEAAEGVGMTERQVLTMVELPLAMPVIMAGIRTAAVWVIGTATLSTPIGQTS
CCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHCCCCCCCC
LGNYIFAGLQTQNWILVLFGCAAAALLALAVDQLLALVEIGLRRGNRWRIGLGTAGIAAI
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHH
VIATLLPGATTRTSYVIGAKTFTEQYVLAALLQQRLESSGLAATTRAGLGSSVIYEALRS
HHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHC
GDIDAYVDYSGTLWASQFKHSGIATRDQVLADLKQDLAKDGVTLLGELGFANAYALVMPR
CCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEEC
GKAEALKIRSIDDLATRAPSMTIAGDYEFFSRPEWANLRSAYGLAFKTERQMQPDFMYAA
CCCCEEEECCHHHHHHCCCCEEEECCHHHHCCCCCHHHHHHHCCEEECCCCCCCCHHHHH
VAAGEVDLIAGYTSDGLIAKYDLVVLDDPKQTIPPYDAVLLLAPKRASDSKLRTALNPLL
HHCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
GRIDIEAMREANLQAGSGGTPDAVARALWERVKPR
HHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLFADPRWRDALANLPDYLGAHVRVSLAALALGLIVSLPLAIFARHRPVLRGTLLGFAG
CCCCCCCHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IVQTIPGLALLALFYPLLLALAALSMSAFGVSFSAFGFLPAVLALALYSMLPVLRNTITG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDGIDPALIEAAEGVGMTERQVLTMVELPLAMPVIMAGIRTAAVWVIGTATLSTPIGQTS
CCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHCCCCCCCC
LGNYIFAGLQTQNWILVLFGCAAAALLALAVDQLLALVEIGLRRGNRWRIGLGTAGIAAI
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHH
VIATLLPGATTRTSYVIGAKTFTEQYVLAALLQQRLESSGLAATTRAGLGSSVIYEALRS
HHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHC
GDIDAYVDYSGTLWASQFKHSGIATRDQVLADLKQDLAKDGVTLLGELGFANAYALVMPR
CCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEEC
GKAEALKIRSIDDLATRAPSMTIAGDYEFFSRPEWANLRSAYGLAFKTERQMQPDFMYAA
CCCCEEEECCHHHHHHCCCCEEEECCHHHHCCCCCHHHHHHHCCEEECCCCCCCCHHHHH
VAAGEVDLIAGYTSDGLIAKYDLVVLDDPKQTIPPYDAVLLLAPKRASDSKLRTALNPLL
HHCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
GRIDIEAMREANLQAGSGGTPDAVARALWERVKPR
HHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10216873; 9384377 [H]