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The map label for this gene is glxR [C]

Identifier: 192290577

GI number: 192290577

Start: 2368072

End: 2368941

Strand: Reverse

Name: glxR [C]

Synonym: Rpal_2186

Alternate gene names: 192290577

Gene position: 2368941-2368072 (Counterclockwise)

Preceding gene: 192290580

Following gene: 192290576

Centisome position: 41.24

GC content: 65.75

Gene sequence:

>870_bases
ATGGCCAAAGTCGCTTTCATCGGTCTCGGCGTTATGGGGTTTCCGATGGCCGGACATCTCGTGACGAAAGGCGGCCATGA
AGTGACGGTGTATAATCGCACGGCCGCGAAGGCGCAGGCCTGGGCCGAGAAGTTCGGCGGCCGCGCCGCGCCGACCCCGG
CAGAGGCCGCAGCCGGCCAGGACTTCGTGATGGCTTGCGTCGGCAACGATGACGACCTGCGTGCCGTCACCACCGGCGCC
GACGGCGCATTCGGCGCGATGAGCAAGGGCGCGATCTTCGTCGACCACACCACGGCTTCAGCCGAAGTCGCGCGCGAGCT
CGACGCGGCTGCCAGCAAGGCCGGCTTCCACTTCATCGACGCGCCGGTGTCCGGCGGCCAAGCCGGTGCCGAAAACGGCG
TGCTGACGGTGATGTGCGGTGGCCGGCCTGAGGATTTCGCCAAGGCCGAACCGGTGATCGGCGCCTATGCGCGGATGTGC
AAGCTGCTGGGCCCCGCAGGCAGCGGTCAGCTCACCAAGATGGTCAATCAGATCTGCATCGCCGGGCTGGTCGAAGGGTT
GTCGGAAGGCATTCACTTCGCCAAGAAGGCCGGCCTCGACGTCAATGCGGTGATCGACACTATCTCCAAGGGCGCGGCGC
AGTCGTGGCAGATGGAGAACCGCCACAAGACCATGAACGATGGCAAATACGATTTCGGCTTCGCGGTCGAATGGATGCGC
AAGGATCTGTCGATCTGCCTCGCCGAAGCGCGCCGCAACGGCGCCAGCCTGCCGGTCACGGCACTGGTCGATACCTTCTA
TGCCGAGGTCGAGAAGATGGGCGGCCGACGCTGGGACACCTCCAGCCTGCTGGCGCGGCTGGAGCGCTGA

Upstream 100 bases:

>100_bases
ATGCTGACGGCTCCTCCCAGCGCGCCTCCCAGCGGGCCGCCCTCACCTCAACTCGATCAGGCGCGACCGGCTCGCGCCCT
CGCAACTGAAGGGTCCAGAC

Downstream 100 bases:

>100_bases
GGCAGCGCGGAACAGGGTTGCAATCGCGGTTTTCACGGCTCGTTCACCGCGATTGTTAGCCAGCTGTTAACCGGAATAAC
TTTCCCTCTAAGGCTGCTCT

Product: 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAKVAFIGLGVMGFPMAGHLVTKGGHEVTVYNRTAAKAQAWAEKFGGRAAPTPAEAAAGQDFVMACVGNDDDLRAVTTGA
DGAFGAMSKGAIFVDHTTASAEVARELDAAASKAGFHFIDAPVSGGQAGAENGVLTVMCGGRPEDFAKAEPVIGAYARMC
KLLGPAGSGQLTKMVNQICIAGLVEGLSEGIHFAKKAGLDVNAVIDTISKGAAQSWQMENRHKTMNDGKYDFGFAVEWMR
KDLSICLAEARRNGASLPVTALVDTFYAEVEKMGGRRWDTSSLLARLER

Sequences:

>Translated_289_residues
MAKVAFIGLGVMGFPMAGHLVTKGGHEVTVYNRTAAKAQAWAEKFGGRAAPTPAEAAAGQDFVMACVGNDDDLRAVTTGA
DGAFGAMSKGAIFVDHTTASAEVARELDAAASKAGFHFIDAPVSGGQAGAENGVLTVMCGGRPEDFAKAEPVIGAYARMC
KLLGPAGSGQLTKMVNQICIAGLVEGLSEGIHFAKKAGLDVNAVIDTISKGAAQSWQMENRHKTMNDGKYDFGFAVEWMR
KDLSICLAEARRNGASLPVTALVDTFYAEVEKMGGRRWDTSSLLARLER
>Mature_288_residues
AKVAFIGLGVMGFPMAGHLVTKGGHEVTVYNRTAAKAQAWAEKFGGRAAPTPAEAAAGQDFVMACVGNDDDLRAVTTGAD
GAFGAMSKGAIFVDHTTASAEVARELDAAASKAGFHFIDAPVSGGQAGAENGVLTVMCGGRPEDFAKAEPVIGAYARMCK
LLGPAGSGQLTKMVNQICIAGLVEGLSEGIHFAKKAGLDVNAVIDTISKGAAQSWQMENRHKTMNDGKYDFGFAVEWMRK
DLSICLAEARRNGASLPVTALVDTFYAEVEKMGGRRWDTSSLLARLER

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=27.6450511945392, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI40556376, Length=281, Percent_Identity=25.2669039145907, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=31.1188811188811, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI145693186, Length=287, Percent_Identity=31.0104529616725, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790315, Length=294, Percent_Identity=28.2312925170068, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1789092, Length=226, Percent_Identity=28.3185840707965, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=28, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24655230, Length=298, Percent_Identity=28.1879194630872, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922568, Length=298, Percent_Identity=28.1879194630872, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=23.0496453900709, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30293; Mature: 30162

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFIGLGVMGFPMAGHLVTKGGHEVTVYNRTAAKAQAWAEKFGGRAAPTPAEAAAGQ
CCEEEEEECCHHHCCCCCCEEECCCCEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHCCC
DFVMACVGNDDDLRAVTTGADGAFGAMSKGAIFVDHTTASAEVARELDAAASKAGFHFID
CEEEEEECCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEE
APVSGGQAGAENGVLTVMCGGRPEDFAKAEPVIGAYARMCKLLGPAGSGQLTKMVNQICI
CCCCCCCCCCCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AGLVEGLSEGIHFAKKAGLDVNAVIDTISKGAAQSWQMENRHKTMNDGKYDFGFAVEWMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KDLSICLAEARRNGASLPVTALVDTFYAEVEKMGGRRWDTSSLLARLER
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AKVAFIGLGVMGFPMAGHLVTKGGHEVTVYNRTAAKAQAWAEKFGGRAAPTPAEAAAGQ
CEEEEEECCHHHCCCCCCEEECCCCEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHCCC
DFVMACVGNDDDLRAVTTGADGAFGAMSKGAIFVDHTTASAEVARELDAAASKAGFHFID
CEEEEEECCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEE
APVSGGQAGAENGVLTVMCGGRPEDFAKAEPVIGAYARMCKLLGPAGSGQLTKMVNQICI
CCCCCCCCCCCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AGLVEGLSEGIHFAKKAGLDVNAVIDTISKGAAQSWQMENRHKTMNDGKYDFGFAVEWMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KDLSICLAEARRNGASLPVTALVDTFYAEVEKMGGRRWDTSSLLARLER
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA