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The map label for this gene is pbpF [H]

Identifier: 192290466

GI number: 192290466

Start: 2242979

End: 2245255

Strand: Reverse

Name: pbpF [H]

Synonym: Rpal_2073

Alternate gene names: 192290466

Gene position: 2245255-2242979 (Counterclockwise)

Preceding gene: 192290469

Following gene: 192290465

Centisome position: 39.09

GC content: 66.8

Gene sequence:

>2277_bases
GTGCCGAAGATTCTGCCAGACAATTGGAAGCAGCGGATCCGGAATTGGTTTCTGGACCTGGACGCCCGGCTCGACTCGAC
GCTGTTCTCATCGGCGGTTGGTATCCGAGAGCTGTGGGAGCGGTTCTCGACCTATATGGACCGCTTCTATGTCGGCGGCT
GGAAGCGCTGGGTGTTCATCGAGCCGCTGTCGGAAGCCGCTTCGATCGGACTGCTCGGCCTCGTCGCGCTGCTGGCGTTG
GCGCAACCGGCATTCCGCGAGACCTCCGACGAGGACTGGCTGAAGAAGTCCGACCTCGCCGTCACCTTCCTCGACCGCTA
CGGTAATCCGATTGGCAGCCGCGGCATCAAACACAACGACGCGATCCCGCTCGAGGACTTCCCGGACAACCTGATCAAGG
CGACGCTCGCGACCGAGGACCGTCGGTTCTACGACCATTTCGGCATCGACGTCGCGGGCACGTTGCGCGCGCTGGTCACC
AATGCCCAGGCCGGCGGCGTGCGCCAGGGCGGCTCCTCGATCAGCCAGCAGCTCGCCAAGAACCTGTTCCTGTCCAACGA
GCGCACGCTCGAACGCAAGATCAACGAAGCGTTCCTGGCGATCTGGCTGGAAACCCGGCTGACCAAGAACGAGATCCTGA
AGCTGTATCTCGACCGCGCCTATATGGGCGGCGGCACCTTCGGCGCCGACGGCGCGGCCCACTTCTACTTCAATAAGTCG
GTGCGCGACGTGAACCTCGCCGAGGCGGCGATGCTCGCCGGCCTGTTCAAGGCGCCGACCAAATACGCCCCGCACATCAA
CCTGCCGGCCGCCCGCGCCCGCGCCAACCAGGTGCTCGACAATCTGGTCGACGCCGGGTTCATGACCGAAGGCCAGGTAT
TCGGCGCCCGCCGCAACCCGGCCTCGGTGGTCGACCGCCGCGACGAGAACTCGCCGAACTACTATCTCGATTGGGCGTTC
GACGAAGTCCGCAAGATCGTCGACACCTTCCCGAAGTCGTACACCGAGCGCGTGTTCGTGGTCCGCACCGCGATTGACAT
GAACGTACAGCGCGCCGCCGAGAGCGCGATCGAGAACCAGCTCCGGCAGTTCGGCCGCGACTATCACGCGACCCAGGCCG
CCACCGTGGTGTCCGATCTCGATGGCGGCGTCCGCGCCATGGTCGGCGGTCGCGACTACGGCTCCAGCCAGTTCAACCGC
GCGGTCGACGCCTATCGCCAGCCGGGTTCGTCGTTCAAGCCTTACGTCTACACCACGGCGCTGCTGAACGGCTTCAAGCC
GACCTCGATCGTGGTCGATGGCCCGGTCTGCCTCGGCAACTGGTGCCCGCAAAACTACGGTCACTCTTATTCCGGCTCGG
TGACGCTGACCCAGGCGATCACCCGCTCGATCAACGTGGTGCCGGTCAAGCTGTCGATCGCGATCGGCGGCAATCCGAAG
AACCAGTGGGACTCGGCCAAGAAGGGCCGCGCCAAGATCGTCGAGGTCGCGCGCCGGTTCGGCCTCACCGCGCCGCTGCT
CGATACTCCGTCGCTACCGATCGGTTCGACCGAAGTCACCGTCGTCGAACACGCGGTCGCCTATGTGACCTTCCCCAACA
AAGGCATGGCGGTGAAGCCGCATGCGGTGCTGGAGATCCGCACCGGCGCCGGCGACTTGGTGTGGCGCTGGGACCGCGAC
GGCCCGCAGCCGCGGCGCGCGATCCCGGCCAACATCGCCTCGGATATGGCCGGGATGATGAGCCACGTCGTCACCGAGGG
CACCGCGCGGCGCGCGCGGCTCGACGGCATTCCGGCGGCTGGCAAGACCGGTACCACCAACGCGTATCGCGACGCCTGGT
TCGTCGGCTACACCGGCAACTTCGCTTGCGCGGTGTGGTACGGCAACGACGACTACTCGCCGACTAACCGGATGACCGGC
GGCTCGCTGCCTGCGCAAACCTGGCACGACATCATGGTGGCGGCGCATCAGGGCGTCGAGGTCAAGGAGCTCGCCGGCGT
CGGCATGGGCGAGAAGCTGCCGGCGACGCCGGGCGAACGCGCGGCGGGCGCCGAGAGCGCCGAAATGAAAGCGGCCCCGC
CGCCGGTGCTGACGCGGCGCGGCGCCGAGATCCTGGTACGGGTGGAGAAGATGCTCGACGAGACCAGCCGTGCACTCGAT
TCCGCCCCTGCACCGGCGACTGGCCGCAAGGTGTCGACCGGGACAGTCGGCTTGACCGACAGCATGGCCGCGGCGGCGCC
CGGCTCCGCCCCGGCTGCGGCGGCCCAGAAGAATTGA

Upstream 100 bases:

>100_bases
TCAGATTCCCAAAGCTCGTCGCCCTCGCCTTGCGATCGCCGCAACATTGCGCAAGACGAACAGCGCCTGTGCGGCGTGCT
GATGTGATTGGTCGAACCCG

Downstream 100 bases:

>100_bases
GCGCGGCGATGCGACTTGCGATGACATCGGGGATAGTTGCGACGTGCGCCTGATCGTCCTCACTCTGCTGGCCCTGCTGA
TCGCCGCCGGCGTCGGCATC

Product: penicillin-binding protein, 1A family

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MPKILPDNWKQRIRNWFLDLDARLDSTLFSSAVGIRELWERFSTYMDRFYVGGWKRWVFIEPLSEAASIGLLGLVALLAL
AQPAFRETSDEDWLKKSDLAVTFLDRYGNPIGSRGIKHNDAIPLEDFPDNLIKATLATEDRRFYDHFGIDVAGTLRALVT
NAQAGGVRQGGSSISQQLAKNLFLSNERTLERKINEAFLAIWLETRLTKNEILKLYLDRAYMGGGTFGADGAAHFYFNKS
VRDVNLAEAAMLAGLFKAPTKYAPHINLPAARARANQVLDNLVDAGFMTEGQVFGARRNPASVVDRRDENSPNYYLDWAF
DEVRKIVDTFPKSYTERVFVVRTAIDMNVQRAAESAIENQLRQFGRDYHATQAATVVSDLDGGVRAMVGGRDYGSSQFNR
AVDAYRQPGSSFKPYVYTTALLNGFKPTSIVVDGPVCLGNWCPQNYGHSYSGSVTLTQAITRSINVVPVKLSIAIGGNPK
NQWDSAKKGRAKIVEVARRFGLTAPLLDTPSLPIGSTEVTVVEHAVAYVTFPNKGMAVKPHAVLEIRTGAGDLVWRWDRD
GPQPRRAIPANIASDMAGMMSHVVTEGTARRARLDGIPAAGKTGTTNAYRDAWFVGYTGNFACAVWYGNDDYSPTNRMTG
GSLPAQTWHDIMVAAHQGVEVKELAGVGMGEKLPATPGERAAGAESAEMKAAPPPVLTRRGAEILVRVEKMLDETSRALD
SAPAPATGRKVSTGTVGLTDSMAAAAPGSAPAAAAQKN

Sequences:

>Translated_758_residues
MPKILPDNWKQRIRNWFLDLDARLDSTLFSSAVGIRELWERFSTYMDRFYVGGWKRWVFIEPLSEAASIGLLGLVALLAL
AQPAFRETSDEDWLKKSDLAVTFLDRYGNPIGSRGIKHNDAIPLEDFPDNLIKATLATEDRRFYDHFGIDVAGTLRALVT
NAQAGGVRQGGSSISQQLAKNLFLSNERTLERKINEAFLAIWLETRLTKNEILKLYLDRAYMGGGTFGADGAAHFYFNKS
VRDVNLAEAAMLAGLFKAPTKYAPHINLPAARARANQVLDNLVDAGFMTEGQVFGARRNPASVVDRRDENSPNYYLDWAF
DEVRKIVDTFPKSYTERVFVVRTAIDMNVQRAAESAIENQLRQFGRDYHATQAATVVSDLDGGVRAMVGGRDYGSSQFNR
AVDAYRQPGSSFKPYVYTTALLNGFKPTSIVVDGPVCLGNWCPQNYGHSYSGSVTLTQAITRSINVVPVKLSIAIGGNPK
NQWDSAKKGRAKIVEVARRFGLTAPLLDTPSLPIGSTEVTVVEHAVAYVTFPNKGMAVKPHAVLEIRTGAGDLVWRWDRD
GPQPRRAIPANIASDMAGMMSHVVTEGTARRARLDGIPAAGKTGTTNAYRDAWFVGYTGNFACAVWYGNDDYSPTNRMTG
GSLPAQTWHDIMVAAHQGVEVKELAGVGMGEKLPATPGERAAGAESAEMKAAPPPVLTRRGAEILVRVEKMLDETSRALD
SAPAPATGRKVSTGTVGLTDSMAAAAPGSAPAAAAQKN
>Mature_757_residues
PKILPDNWKQRIRNWFLDLDARLDSTLFSSAVGIRELWERFSTYMDRFYVGGWKRWVFIEPLSEAASIGLLGLVALLALA
QPAFRETSDEDWLKKSDLAVTFLDRYGNPIGSRGIKHNDAIPLEDFPDNLIKATLATEDRRFYDHFGIDVAGTLRALVTN
AQAGGVRQGGSSISQQLAKNLFLSNERTLERKINEAFLAIWLETRLTKNEILKLYLDRAYMGGGTFGADGAAHFYFNKSV
RDVNLAEAAMLAGLFKAPTKYAPHINLPAARARANQVLDNLVDAGFMTEGQVFGARRNPASVVDRRDENSPNYYLDWAFD
EVRKIVDTFPKSYTERVFVVRTAIDMNVQRAAESAIENQLRQFGRDYHATQAATVVSDLDGGVRAMVGGRDYGSSQFNRA
VDAYRQPGSSFKPYVYTTALLNGFKPTSIVVDGPVCLGNWCPQNYGHSYSGSVTLTQAITRSINVVPVKLSIAIGGNPKN
QWDSAKKGRAKIVEVARRFGLTAPLLDTPSLPIGSTEVTVVEHAVAYVTFPNKGMAVKPHAVLEIRTGAGDLVWRWDRDG
PQPRRAIPANIASDMAGMMSHVVTEGTARRARLDGIPAAGKTGTTNAYRDAWFVGYTGNFACAVWYGNDDYSPTNRMTGG
SLPAQTWHDIMVAAHQGVEVKELAGVGMGEKLPATPGERAAGAESAEMKAAPPPVLTRRGAEILVRVEKMLDETSRALDS
APAPATGRKVSTGTVGLTDSMAAAAPGSAPAAAAQKN

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=542, Percent_Identity=33.0258302583026, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI87082258, Length=258, Percent_Identity=38.3720930232558, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788867, Length=526, Percent_Identity=28.7072243346008, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1789601, Length=185, Percent_Identity=35.1351351351351, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 82601; Mature: 82470

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKILPDNWKQRIRNWFLDLDARLDSTLFSSAVGIRELWERFSTYMDRFYVGGWKRWVFI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
EPLSEAASIGLLGLVALLALAQPAFRETSDEDWLKKSDLAVTFLDRYGNPIGSRGIKHND
CCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHCCCEEEEHHHHCCCCCCCCCCCCC
AIPLEDFPDNLIKATLATEDRRFYDHFGIDVAGTLRALVTNAQAGGVRQGGSSISQQLAK
CCCCCCCCHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
NLFLSNERTLERKINEAFLAIWLETRLTKNEILKLYLDRAYMGGGTFGADGAAHFYFNKS
HHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
VRDVNLAEAAMLAGLFKAPTKYAPHINLPAARARANQVLDNLVDAGFMTEGQVFGARRNP
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCC
ASVVDRRDENSPNYYLDWAFDEVRKIVDTFPKSYTERVFVVRTAIDMNVQRAAESAIENQ
HHHHCCCCCCCCCEEEECCHHHHHHHHHHHCHHHHCEEEEEEEHHHCHHHHHHHHHHHHH
LRQFGRDYHATQAATVVSDLDGGVRAMVGGRDYGSSQFNRAVDAYRQPGSSFKPYVYTTA
HHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEHH
LLNGFKPTSIVVDGPVCLGNWCPQNYGHSYSGSVTLTQAITRSINVVPVKLSIAIGGNPK
HHCCCCCCEEEEECCEEECCCCCCCCCCCCCCCEEHHHHHHCCCEEEEEEEEEEECCCCC
NQWDSAKKGRAKIVEVARRFGLTAPLLDTPSLPIGSTEVTVVEHAVAYVTFPNKGMAVKP
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHEEEEEEEEEEECCCCCEECC
HAVLEIRTGAGDLVWRWDRDGPQPRRAIPANIASDMAGMMSHVVTEGTARRARLDGIPAA
CEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
GKTGTTNAYRDAWFVGYTGNFACAVWYGNDDYSPTNRMTGGSLPAQTWHDIMVAAHQGVE
CCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VKELAGVGMGEKLPATPGERAAGAESAEMKAAPPPVLTRRGAEILVRVEKMLDETSRALD
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
SAPAPATGRKVSTGTVGLTDSMAAAAPGSAPAAAAQKN
CCCCCCCCCEECCCCCCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PKILPDNWKQRIRNWFLDLDARLDSTLFSSAVGIRELWERFSTYMDRFYVGGWKRWVFI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
EPLSEAASIGLLGLVALLALAQPAFRETSDEDWLKKSDLAVTFLDRYGNPIGSRGIKHND
CCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHCCCEEEEHHHHCCCCCCCCCCCCC
AIPLEDFPDNLIKATLATEDRRFYDHFGIDVAGTLRALVTNAQAGGVRQGGSSISQQLAK
CCCCCCCCHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
NLFLSNERTLERKINEAFLAIWLETRLTKNEILKLYLDRAYMGGGTFGADGAAHFYFNKS
HHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
VRDVNLAEAAMLAGLFKAPTKYAPHINLPAARARANQVLDNLVDAGFMTEGQVFGARRNP
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCC
ASVVDRRDENSPNYYLDWAFDEVRKIVDTFPKSYTERVFVVRTAIDMNVQRAAESAIENQ
HHHHCCCCCCCCCEEEECCHHHHHHHHHHHCHHHHCEEEEEEEHHHCHHHHHHHHHHHHH
LRQFGRDYHATQAATVVSDLDGGVRAMVGGRDYGSSQFNRAVDAYRQPGSSFKPYVYTTA
HHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEHH
LLNGFKPTSIVVDGPVCLGNWCPQNYGHSYSGSVTLTQAITRSINVVPVKLSIAIGGNPK
HHCCCCCCEEEEECCEEECCCCCCCCCCCCCCCEEHHHHHHCCCEEEEEEEEEEECCCCC
NQWDSAKKGRAKIVEVARRFGLTAPLLDTPSLPIGSTEVTVVEHAVAYVTFPNKGMAVKP
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHEEEEEEEEEEECCCCCEECC
HAVLEIRTGAGDLVWRWDRDGPQPRRAIPANIASDMAGMMSHVVTEGTARRARLDGIPAA
CEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
GKTGTTNAYRDAWFVGYTGNFACAVWYGNDDYSPTNRMTGGSLPAQTWHDIMVAAHQGVE
CCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VKELAGVGMGEKLPATPGERAAGAESAEMKAAPPPVLTRRGAEILVRVEKMLDETSRALD
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
SAPAPATGRKVSTGTVGLTDSMAAAAPGSAPAAAAQKN
CCCCCCCCCEECCCCCCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]