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The map label for this gene is pleD [H]

Identifier: 192290414

GI number: 192290414

Start: 2192773

End: 2193918

Strand: Reverse

Name: pleD [H]

Synonym: Rpal_2021

Alternate gene names: 192290414

Gene position: 2193918-2192773 (Counterclockwise)

Preceding gene: 192290422

Following gene: 192290413

Centisome position: 38.19

GC content: 64.05

Gene sequence:

>1146_bases
ATGGGACCAACTGCCGCGCTGTCGGGCACCCTGCCCGATCCTCAAACCAGGGCCGAAGACGCGCGCACCCTTGCCGTCCG
CGGCCGGCGGATGCGTCAGCGCCGGCACATGCTGGACCTGATCGCCGCCAGCTTCATGATCGATGCCGCGATCCTGCTGA
TCTACGTCCAAGCCGGGACAATCCCGTCGATGGTGGTGGTGGCGTTCGCCGTCTCCGGCGCCCTGGTCGGTGGCATCCTG
TTCGCTCTGTCGGAATCCGGATTCAACGATCGCTTCTCCGATCACTATCTGACGGTGCCGATCGCGATCACGACGCTGGC
ACTGATCATCGCCTTCACGGTATGGGCGCCGCAGATCGGGGCGTTCTTCCTGTTCGAACTGTTCGTCGTGTTCGGCTTCG
CGTCGCTGCGCGCCGACCGTCGGCAGGCGCTGATCCTCTGGTCCGTGCTGCTGTGCGTGCTGGTGCCGCTGTTCCTGCTC
ACCGATCTGCCGATCGGGATGCCGCACGACAATTCGCTCGAACGCCTCGCCACCCTGCTGGCGCTGGTGCTGACGGTCGG
ACGCTGCACCTTCATCGGGGTGTTTTCGAAAGCCTTGCGCGATTCGCTGTACAAGCGCGGAGTCCAGCTGCGCGAAGCCT
ATCAGCGGATCGAGGAACTGGCCGAACTCGACGAACTGACCGGGGCCTATAACCGCCGCTGTATCATGCGGCACCTGGAC
AATGAGATCGAACGCGCGGCGCGGCACTGCGAGTCGCTGTCGCTGGCGTTGATCGACCTCGACTGGTTCAAGCGCATCAA
TGATACCTTTGGCCATCCGATCGGCGACGAGGTGCTGCGCACCTTCGCTATCACCATCTTCGCCAACATCCGCAGCTTCG
ACCGGTTCGGCCGCTACGGCGGCGAAGAATTCCTGCTGCTGCTGCCGAACACATCCGACGACGAAGCCCGCCTGATCCTC
GACCGGCTTCGCATGATCGTCGCCGATCTCGACTGGAGCGCGTTCTCGCCCAGCATGATGGTGACGCTGTCCGCCGGCGT
CACCACCATGACGCTCCAAGAATCCACCGAAGCCGTGCTGGCACGGGCCGATCGCGCGCTCTACGAGGCCAAGCACGGCG
GACGAAACCGAATTTCCTGCGCCTGA

Upstream 100 bases:

>100_bases
TACTAGCGGCCTCTATCAACCCTTGCGAGCAAATGGCAGATACCAAAACGAAAGGCTTTTCCTAGTAGGTTCCCCAAGAG
CAAAATCGACCGGGCGGACG

Downstream 100 bases:

>100_bases
AGAAACAGGCGTTCTGTGTCCGCTCTAGGAAAGAGTAATGACTTCACGACCAAAGAGGCCGGCCCTTCACCTGCTCGATG
AATTGCAGGCTATGCTGGCG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MGPTAALSGTLPDPQTRAEDARTLAVRGRRMRQRRHMLDLIAASFMIDAAILLIYVQAGTIPSMVVVAFAVSGALVGGIL
FALSESGFNDRFSDHYLTVPIAITTLALIIAFTVWAPQIGAFFLFELFVVFGFASLRADRRQALILWSVLLCVLVPLFLL
TDLPIGMPHDNSLERLATLLALVLTVGRCTFIGVFSKALRDSLYKRGVQLREAYQRIEELAELDELTGAYNRRCIMRHLD
NEIERAARHCESLSLALIDLDWFKRINDTFGHPIGDEVLRTFAITIFANIRSFDRFGRYGGEEFLLLLPNTSDDEARLIL
DRLRMIVADLDWSAFSPSMMVTLSAGVTTMTLQESTEAVLARADRALYEAKHGGRNRISCA

Sequences:

>Translated_381_residues
MGPTAALSGTLPDPQTRAEDARTLAVRGRRMRQRRHMLDLIAASFMIDAAILLIYVQAGTIPSMVVVAFAVSGALVGGIL
FALSESGFNDRFSDHYLTVPIAITTLALIIAFTVWAPQIGAFFLFELFVVFGFASLRADRRQALILWSVLLCVLVPLFLL
TDLPIGMPHDNSLERLATLLALVLTVGRCTFIGVFSKALRDSLYKRGVQLREAYQRIEELAELDELTGAYNRRCIMRHLD
NEIERAARHCESLSLALIDLDWFKRINDTFGHPIGDEVLRTFAITIFANIRSFDRFGRYGGEEFLLLLPNTSDDEARLIL
DRLRMIVADLDWSAFSPSMMVTLSAGVTTMTLQESTEAVLARADRALYEAKHGGRNRISCA
>Mature_380_residues
GPTAALSGTLPDPQTRAEDARTLAVRGRRMRQRRHMLDLIAASFMIDAAILLIYVQAGTIPSMVVVAFAVSGALVGGILF
ALSESGFNDRFSDHYLTVPIAITTLALIIAFTVWAPQIGAFFLFELFVVFGFASLRADRRQALILWSVLLCVLVPLFLLT
DLPIGMPHDNSLERLATLLALVLTVGRCTFIGVFSKALRDSLYKRGVQLREAYQRIEELAELDELTGAYNRRCIMRHLDN
EIERAARHCESLSLALIDLDWFKRINDTFGHPIGDEVLRTFAITIFANIRSFDRFGRYGGEEFLLLLPNTSDDEARLILD
RLRMIVADLDWSAFSPSMMVTLSAGVTTMTLQESTEAVLARADRALYEAKHGGRNRISCA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=34.6368715083799, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=38.3647798742138, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693134, Length=378, Percent_Identity=26.1904761904762, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=38.9221556886228, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=34.7560975609756, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787802, Length=223, Percent_Identity=30.0448430493274, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082007, Length=158, Percent_Identity=37.3417721518987, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=33.9181286549708, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=33.1395348837209, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788085, Length=170, Percent_Identity=34.1176470588235, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=164, Percent_Identity=26.8292682926829, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787056, Length=187, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42448; Mature: 42317

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPTAALSGTLPDPQTRAEDARTLAVRGRRMRQRRHMLDLIAASFMIDAAILLIYVQAGT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPSMVVVAFAVSGALVGGILFALSESGFNDRFSDHYLTVPIAITTLALIIAFTVWAPQIG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
AFFLFELFVVFGFASLRADRRQALILWSVLLCVLVPLFLLTDLPIGMPHDNSLERLATLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
ALVLTVGRCTFIGVFSKALRDSLYKRGVQLREAYQRIEELAELDELTGAYNRRCIMRHLD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEIERAARHCESLSLALIDLDWFKRINDTFGHPIGDEVLRTFAITIFANIRSFDRFGRYG
HHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GEEFLLLLPNTSDDEARLILDRLRMIVADLDWSAFSPSMMVTLSAGVTTMTLQESTEAVL
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEHHHHHHHHH
ARADRALYEAKHGGRNRISCA
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GPTAALSGTLPDPQTRAEDARTLAVRGRRMRQRRHMLDLIAASFMIDAAILLIYVQAGT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPSMVVVAFAVSGALVGGILFALSESGFNDRFSDHYLTVPIAITTLALIIAFTVWAPQIG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
AFFLFELFVVFGFASLRADRRQALILWSVLLCVLVPLFLLTDLPIGMPHDNSLERLATLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
ALVLTVGRCTFIGVFSKALRDSLYKRGVQLREAYQRIEELAELDELTGAYNRRCIMRHLD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEIERAARHCESLSLALIDLDWFKRINDTFGHPIGDEVLRTFAITIFANIRSFDRFGRYG
HHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GEEFLLLLPNTSDDEARLILDRLRMIVADLDWSAFSPSMMVTLSAGVTTMTLQESTEAVL
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEHHHHHHHHH
ARADRALYEAKHGGRNRISCA
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA