BacMap

Definition	Rhodopseudomonas palustris TIE-1 chromosome, complete genome.
Accession	NC_011004
Length	5,744,041

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The map label for this gene is caiD [H]

Identifier: 192290355

GI number: 192290355

Start: 2117068

End: 2117847

Strand: Reverse

Name: caiD [H]

Synonym: Rpal_1962

Alternate gene names: 192290355

Gene position: 2117847-2117068 (Counterclockwise)

Preceding gene: 192290357

Following gene: 192290354

Centisome position: 36.87

GC content: 67.05

Gene sequence:

>780_bases
ATGGCGCTGCAATTCTCCACCGTCACCCGCAAGGGGCCGATCACCATCGTCACGCTGTCGCGGCCCGAAGTGTACAACGC
GCTGCACACCGACGCGCATTTCGAACTCGAAGGCGTGTTCAACGATTTCGCCGCCGATCCCGAGCAGTGGGTGGCGATCG
TCACCGGCGCCGGCGACAAGGCGTTCTGCGCCGGCAACGATCTGAAGTGGCAGGCCTCCGGCGGCAAGCGCGGCTGGGGC
GAGAGCGGCTTCGGCGGCCTGACCTCGCGGTTCGATTGCGACAAGCCGATTATCGCTGCCGTCAACGGCGTCGCGATGGG
CGGCGGTTTCGAGATCGCGCTTGCCTGCGATCTGATCATCGCTGCGGAGAACGCGACCTTCGCACTGCCGGAGCCGCGCG
TCGGCCTCGCGGCGCTGGCCGGCGGCCTGCACCGCCTCCCCCGCCAGATCGGGCTGAAGCGCGCGATGGGCATGATCCTC
ACCGCCCGCCACGTCAAGGCGCAGGAAGGCTATGAGCTCGGCTTCGTCAACGAAGTCGTGCCGCAAGGCCAGGCGCTCGC
CGCGGCGGAACGCTGGGCCGAAACCATCTGCACCGTCTCACCGATGTCGATCCGCGCCTCGAAGCAGACGCTGTCGCGCG
GCCTCGAAGTGTCACTGCCGCAAGCGATCGCCGAGCAGCGCGACTACCCGGCAGTGAAAGCCATGGTCGCCTCGCAGGAC
TACATCGAAGGCCCGAAAGCGTTCTCCGAAAAGCGCAAGCCGAACTGGAGCGGGAAATAG

Upstream 100 bases:

>100_bases
TTGTTGATCTTGTTAAGCGACCGATTAACATGCGCCGCACGAGACAAGTCAAGCGCGCTTCAGCCGCACCAATTCTGCTC
AACGAAAAAAGGAAACGCCG

Downstream 100 bases:

>100_bases
CCCCCACCAAGTAATGACTTGATGGCCCTAGGTGGGAATGATAGCAAGGCGTCCGATTAGAGTTAGTGGCTTCGGACGCC
TTGTAGCGGATTACCTTAGC

Product: enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MALQFSTVTRKGPITIVTLSRPEVYNALHTDAHFELEGVFNDFAADPEQWVAIVTGAGDKAFCAGNDLKWQASGGKRGWG
ESGFGGLTSRFDCDKPIIAAVNGVAMGGGFEIALACDLIIAAENATFALPEPRVGLAALAGGLHRLPRQIGLKRAMGMIL
TARHVKAQEGYELGFVNEVVPQGQALAAAERWAETICTVSPMSIRASKQTLSRGLEVSLPQAIAEQRDYPAVKAMVASQD
YIEGPKAFSEKRKPNWSGK

Sequences:

>Translated_259_residues
MALQFSTVTRKGPITIVTLSRPEVYNALHTDAHFELEGVFNDFAADPEQWVAIVTGAGDKAFCAGNDLKWQASGGKRGWG
ESGFGGLTSRFDCDKPIIAAVNGVAMGGGFEIALACDLIIAAENATFALPEPRVGLAALAGGLHRLPRQIGLKRAMGMIL
TARHVKAQEGYELGFVNEVVPQGQALAAAERWAETICTVSPMSIRASKQTLSRGLEVSLPQAIAEQRDYPAVKAMVASQD
YIEGPKAFSEKRKPNWSGK
>Mature_258_residues
ALQFSTVTRKGPITIVTLSRPEVYNALHTDAHFELEGVFNDFAADPEQWVAIVTGAGDKAFCAGNDLKWQASGGKRGWGE
SGFGGLTSRFDCDKPIIAAVNGVAMGGGFEIALACDLIIAAENATFALPEPRVGLAALAGGLHRLPRQIGLKRAMGMILT
ARHVKAQEGYELGFVNEVVPQGQALAAAERWAETICTVSPMSIRASKQTLSRGLEVSLPQAIAEQRDYPAVKAMVASQDY
IEGPKAFSEKRKPNWSGK

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=34.6613545816733, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI70995211, Length=255, Percent_Identity=31.7647058823529, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=30.5019305019305, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI20127408, Length=236, Percent_Identity=30.0847457627119, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=29.7619047619048, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI37594469, Length=189, Percent_Identity=31.7460317460317, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI37594471, Length=189, Percent_Identity=31.7460317460317, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI150378541, Length=250, Percent_Identity=28.8, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI68989263, Length=190, Percent_Identity=31.5789473684211, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI157694516, Length=226, Percent_Identity=27.8761061946903, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI260274832, Length=226, Percent_Identity=25.2212389380531, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI45643119, Length=226, Percent_Identity=25.2212389380531, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI260275230, Length=226, Percent_Identity=25.2212389380531, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI213417737, Length=226, Percent_Identity=27.8761061946903, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI25453481, Length=248, Percent_Identity=24.1935483870968, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI51538573, Length=248, Percent_Identity=24.1935483870968, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=42.8571428571429, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=34.7826086956522, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=31.8725099601594, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=28.4615384615385, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787660, Length=256, Percent_Identity=33.203125, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=28.3582089552239, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790281, Length=89, Percent_Identity=39.3258426966292, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=34.4961240310078, Blast_Score=147, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=34.9019607843137, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17560910, Length=258, Percent_Identity=30.2325581395349, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=224, Percent_Identity=30.8035714285714, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=259, Percent_Identity=27.7992277992278, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=211, Percent_Identity=30.8056872037915, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=234, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=215, Percent_Identity=27.4418604651163, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=218, Percent_Identity=25.6880733944954, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=218, Percent_Identity=26.605504587156, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=218, Percent_Identity=26.605504587156, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=218, Percent_Identity=26.605504587156, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=192, Percent_Identity=26.0416666666667, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=35.0806451612903, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=35.0806451612903, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=33.8461538461538, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21357171, Length=265, Percent_Identity=30.5660377358491, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19920382, Length=217, Percent_Identity=29.9539170506912, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24650670, Length=201, Percent_Identity=30.8457711442786, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24654903, Length=177, Percent_Identity=27.1186440677966, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921000, Length=248, Percent_Identity=27.8225806451613, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583077, Length=248, Percent_Identity=27.8225806451613, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583079, Length=248, Percent_Identity=27.8225806451613, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571730, Length=167, Percent_Identity=27.5449101796407, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571729, Length=167, Percent_Identity=27.5449101796407, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922422, Length=244, Percent_Identity=24.5901639344262, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 27687; Mature: 27556

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQFSTVTRKGPITIVTLSRPEVYNALHTDAHFELEGVFNDFAADPEQWVAIVTGAGDK
CCEEECCCCCCCCEEEEEECCCHHHHHHHCCCCEEEHHHHHHHCCCCCCEEEEEECCCCC
AFCAGNDLKWQASGGKRGWGESGFGGLTSRFDCDKPIIAAVNGVAMGGGFEIALACDLII
EEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCEEEEEEEEEEE
AAENATFALPEPRVGLAALAGGLHRLPRQIGLKRAMGMILTARHVKAQEGYELGFVNEVV
EECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
PQGQALAAAERWAETICTVSPMSIRASKQTLSRGLEVSLPQAIAEQRDYPAVKAMVASQD
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEECHHHHHHCCCCCHHHHHHHCCH
YIEGPKAFSEKRKPNWSGK
HHCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
ALQFSTVTRKGPITIVTLSRPEVYNALHTDAHFELEGVFNDFAADPEQWVAIVTGAGDK
CEEECCCCCCCCEEEEEECCCHHHHHHHCCCCEEEHHHHHHHCCCCCCEEEEEECCCCC
AFCAGNDLKWQASGGKRGWGESGFGGLTSRFDCDKPIIAAVNGVAMGGGFEIALACDLII
EEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCEEEEEEEEEEE
AAENATFALPEPRVGLAALAGGLHRLPRQIGLKRAMGMILTARHVKAQEGYELGFVNEVV
EECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
PQGQALAAAERWAETICTVSPMSIRASKQTLSRGLEVSLPQAIAEQRDYPAVKAMVASQD
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEECHHHHHHCCCCCHHHHHHHCCH
YIEGPKAFSEKRKPNWSGK
HHCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA