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The map label for this gene is hemN [H]

Identifier: 192290258

GI number: 192290258

Start: 1998764

End: 2000113

Strand: Reverse

Name: hemN [H]

Synonym: Rpal_1862

Alternate gene names: 192290258

Gene position: 2000113-1998764 (Counterclockwise)

Preceding gene: 192290259

Following gene: 192290257

Centisome position: 34.82

GC content: 66.07

Gene sequence:

>1350_bases
ATGTCGTCTGCCCTCGACAAATACGCCAAGAGCTCGGTGCCGCGCTACACCAGCTATCCCACCGCACCGCATTTCGCGAA
GGACTTCCCGGAGTCGATCTATCGCGGCTGGCTCGCCCAGCTCGACACCGACGAGCCGGTTTCGCTGTATCTTCATGTGC
CGTTCTGCAAGCAGATGTGCTGGTACTGCGGCTGCAACATGAAGCTGGCGGCGAAGTACGATCCGGTCGCCGACTACGTC
GAGCACCTGATCGACGAAATCGATCTGGTCGCGGACGCTCTCCCCGGCACCATGCCGGTGCGTCATCTGCATTTCGGCGG
CGGCACCCCGACGGTGATCGATCCGCAGGATCTCGGCGCGCTGATGACGCTGCTGCGCGAGCGCTTCGAGTTCCTGCCCG
ATGCCGAGATCGCGATCGAGAGCGACCCGCGCACGCTGACCGAAGACATGGCCGCCAAGATCGGCGAGCTCGGCTTCACC
CGCGCCAGCTTCGGCGTGCAGGAATTCGACCCGAAGGTGCAGGAAGCGATCAACCGCGTCCAGCCGCCCGAAATGGTCGC
GCGCGCGATGCAGCTGTTCAAATCGGCCGGCGTCGAGCGCATCAATTTCGACCTGATCTACGGCCTGCCCTATCAGACCG
CCGAAGACCTGCGCCGCACCGTCGAACAGTGCGTCGAGATGAAGCCCGACCGGGTCGCGCTATTCGGCTACGCTCACGTG
CCGTGGGTCGCCAAGAACCAGCGGATGATCCCGGACGAGTCGCTGCCGAAGCCGGAGCTGCGCGCCACGCAGGCCGAGAC
CGCTGCCGAAGCCCTGGTGAAGGGCGGCTACGTCCGCATCGGCATCGACCATTTCGCGCTGCCGGGTGACTCGCTCGCGA
TCGCGGCCAAGACCGGCGAACTGCACCGGAATTTCCAGGGCTACACCTCCGACGCGGCGCAGACCCTGATCGGCCTGGGC
GCCACATCGATCGGCCGCACCCCGAGCGGCTATGTGCAGAACATCAGCGAAACCGGTGCCTGGTCGCGCGCGGTCGAAGC
CGGCAAGATCCCGGTCGCGCGTGGTCACGCTCTGACCCAGCAGGACAATCTGCGCGCCCACGTGATCGAACGCATCATGT
GCGACGGCAAGGTCGACCTCGCCGCAGCCGGCAAGGCCTTCGGCTGTGGCGAAGACTGGTACGCGCCGGAGCAGGACTCG
CTCGCCGAACTGCAGCGCGACGGCGCCGTGGTGTGCAATGGCAGCAAGCTGACGCTGACGCCGGAAGGCGTCCGGCTGTC
GCGTGTGGTGGCGTCGGTGTTCGACACCTACCTGCGCAACTCGTCGGTCCGGCACTCGATCGCGGTCTGA

Upstream 100 bases:

>100_bases
GCCGCCAATATTTACGCCAGCAGGCGATCAGAGCCGATCGCAGGCCAGTCAGAAACCCTTTCCGCGCGTTGATCCGCGGG
CGATCAGAGAGATCAGGACG

Downstream 100 bases:

>100_bases
CCAGCCCTACAATTTTACCAGCTACACAAACGCGCGCTTCGGCGCGCGTTTGTCGTTTTGATACAGCTCAATTGCAATTT
CGCGGTTGGGTGGATTATTA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSSALDKYAKSSVPRYTSYPTAPHFAKDFPESIYRGWLAQLDTDEPVSLYLHVPFCKQMCWYCGCNMKLAAKYDPVADYV
EHLIDEIDLVADALPGTMPVRHLHFGGGTPTVIDPQDLGALMTLLRERFEFLPDAEIAIESDPRTLTEDMAAKIGELGFT
RASFGVQEFDPKVQEAINRVQPPEMVARAMQLFKSAGVERINFDLIYGLPYQTAEDLRRTVEQCVEMKPDRVALFGYAHV
PWVAKNQRMIPDESLPKPELRATQAETAAEALVKGGYVRIGIDHFALPGDSLAIAAKTGELHRNFQGYTSDAAQTLIGLG
ATSIGRTPSGYVQNISETGAWSRAVEAGKIPVARGHALTQQDNLRAHVIERIMCDGKVDLAAAGKAFGCGEDWYAPEQDS
LAELQRDGAVVCNGSKLTLTPEGVRLSRVVASVFDTYLRNSSVRHSIAV

Sequences:

>Translated_449_residues
MSSALDKYAKSSVPRYTSYPTAPHFAKDFPESIYRGWLAQLDTDEPVSLYLHVPFCKQMCWYCGCNMKLAAKYDPVADYV
EHLIDEIDLVADALPGTMPVRHLHFGGGTPTVIDPQDLGALMTLLRERFEFLPDAEIAIESDPRTLTEDMAAKIGELGFT
RASFGVQEFDPKVQEAINRVQPPEMVARAMQLFKSAGVERINFDLIYGLPYQTAEDLRRTVEQCVEMKPDRVALFGYAHV
PWVAKNQRMIPDESLPKPELRATQAETAAEALVKGGYVRIGIDHFALPGDSLAIAAKTGELHRNFQGYTSDAAQTLIGLG
ATSIGRTPSGYVQNISETGAWSRAVEAGKIPVARGHALTQQDNLRAHVIERIMCDGKVDLAAAGKAFGCGEDWYAPEQDS
LAELQRDGAVVCNGSKLTLTPEGVRLSRVVASVFDTYLRNSSVRHSIAV
>Mature_448_residues
SSALDKYAKSSVPRYTSYPTAPHFAKDFPESIYRGWLAQLDTDEPVSLYLHVPFCKQMCWYCGCNMKLAAKYDPVADYVE
HLIDEIDLVADALPGTMPVRHLHFGGGTPTVIDPQDLGALMTLLRERFEFLPDAEIAIESDPRTLTEDMAAKIGELGFTR
ASFGVQEFDPKVQEAINRVQPPEMVARAMQLFKSAGVERINFDLIYGLPYQTAEDLRRTVEQCVEMKPDRVALFGYAHVP
WVAKNQRMIPDESLPKPELRATQAETAAEALVKGGYVRIGIDHFALPGDSLAIAAKTGELHRNFQGYTSDAAQTLIGLGA
TSIGRTPSGYVQNISETGAWSRAVEAGKIPVARGHALTQQDNLRAHVIERIMCDGKVDLAAAGKAFGCGEDWYAPEQDSL
AELQRDGAVVCNGSKLTLTPEGVRLSRVVASVFDTYLRNSSVRHSIAV

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=252, Percent_Identity=25, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI87082341, Length=440, Percent_Identity=38.6363636363636, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1789325, Length=244, Percent_Identity=29.5081967213115, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 49339; Mature: 49207

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSALDKYAKSSVPRYTSYPTAPHFAKDFPESIYRGWLAQLDTDEPVSLYLHVPFCKQMC
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHH
WYCGCNMKLAAKYDPVADYVEHLIDEIDLVADALPGTMPVRHLHFGGGTPTVIDPQDLGA
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEECHHHHHH
LMTLLRERFEFLPDAEIAIESDPRTLTEDMAAKIGELGFTRASFGVQEFDPKVQEAINRV
HHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCHHHHCCHHHCCHHHHHHHHHC
QPPEMVARAMQLFKSAGVERINFDLIYGLPYQTAEDLRRTVEQCVEMKPDRVALFGYAHV
CCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEC
PWVAKNQRMIPDESLPKPELRATQAETAAEALVKGGYVRIGIDHFALPGDSLAIAAKTGE
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECHHEECCCCCEEEEEECCH
LHRNFQGYTSDAAQTLIGLGATSIGRTPSGYVQNISETGAWSRAVEAGKIPVARGHALTQ
HHHCCCCCCHHHHHHHHHCCCHHCCCCCCHHHHCCHHCCHHHHHHHCCCCCCCCCCCCCC
QDNLRAHVIERIMCDGKVDLAAAGKAFGCGEDWYAPEQDSLAELQRDGAVVCNGSKLTLT
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCEEEEC
PEGVRLSRVVASVFDTYLRNSSVRHSIAV
CCCCHHHHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure 
SSALDKYAKSSVPRYTSYPTAPHFAKDFPESIYRGWLAQLDTDEPVSLYLHVPFCKQMC
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHH
WYCGCNMKLAAKYDPVADYVEHLIDEIDLVADALPGTMPVRHLHFGGGTPTVIDPQDLGA
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEECHHHHHH
LMTLLRERFEFLPDAEIAIESDPRTLTEDMAAKIGELGFTRASFGVQEFDPKVQEAINRV
HHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCHHHHCCHHHCCHHHHHHHHHC
QPPEMVARAMQLFKSAGVERINFDLIYGLPYQTAEDLRRTVEQCVEMKPDRVALFGYAHV
CCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEC
PWVAKNQRMIPDESLPKPELRATQAETAAEALVKGGYVRIGIDHFALPGDSLAIAAKTGE
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECHHEECCCCCEEEEEECCH
LHRNFQGYTSDAAQTLIGLGATSIGRTPSGYVQNISETGAWSRAVEAGKIPVARGHALTQ
HHHCCCCCCHHHHHHHHHCCCHHCCCCCCHHHHCCHHCCHHHHHHHCCCCCCCCCCCCCC
QDNLRAHVIERIMCDGKVDLAAAGKAFGCGEDWYAPEQDSLAELQRDGAVVCNGSKLTLT
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCEEEEC
PEGVRLSRVVASVFDTYLRNSSVRHSIAV
CCCCHHHHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157943; 12597275 [H]