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The map label for this gene is mdeA [H]

Identifier: 192290155

GI number: 192290155

Start: 1885931

End: 1887226

Strand: Reverse

Name: mdeA [H]

Synonym: Rpal_1757

Alternate gene names: 192290155

Gene position: 1887226-1885931 (Counterclockwise)

Preceding gene: 192290156

Following gene: 192290154

Centisome position: 32.86

GC content: 66.28

Gene sequence:

>1296_bases
ATGGCCGCACCCAAACCGCCCGGATTTGAAACCCTCAGCCTGCACGCCGGGCAACATCCCGATCCGCTGACCGGCTCGCG
CGCGGTGCCGATCTATCAGACCACGTCCTACGTGTTTCAGGACACCGACCACGCCGCGGCGCTGTTCAATATGGAGCGGC
CCGGCCACCTCTACACGCGGATCTCCAACCCGACGATCGCGGTGCTGGAAGAGCGGATCGCCGCGCTGGAGAACGGCGTC
GGCGCGGTGGCGACCGCCAGCGGCATGGCGGCGCTGCATCTGGCGATCGCGACGCTGCTGAATGCCGGCGATCACATCGT
CGCGTCGTCGTCGCTGTACGGCGGCACGATCAACCTCTTGGCGCACACGCTGCCGCGGTTCGGCATCACCACCACCTTCG
TGGCGCCTCGCGACCACGCCGGCCTCGCAGCGGCGATCCAGCCGAACACCAGGCTGGTGATCGGCGAGACCATCGGCAAT
CCGGGCCTCGAAGTGCTCGACATCCCGAAGGTCGCGGCCATCGCCCATGAGGCGAAAATCCCGCTCTTGATCGACAACAC
CTTCGCGACGCCGTACCTCAGCAAGCCGATCGAGCTCGGCGCCGACATCGTGATGCATTCGGTCACAAAATGGCTCGGCG
GCCACGGCATCGCCATCGGCGGCGTGCTGGTCGACGGCGGCCGGTTCGACTGGCGCGGCTCCGGCAAGTTTCCGACGCTG
ACCGAGCCCTACGCCGGCTATCACGACGTCGTCTTCGACGAGCAGTTCGGCCCGCCCGCTTTCGTCATTCGCGCGCGGAT
GGAAGGTCTGCGCGATTTCGGCGCCTGCCTGTCGCCGACCAACGCTTTCCAGCTGCTGCAGGGCATCGAGACGCTGCCGG
TTCGGATGGATCGGCATGTCGCCAACACCAAGGCGGTGCTCGACTTCCTTCAGACCAACAAGGCGGTCGATTGGGTTCTG
CATCCGACGCTGGACAACCACCCCGACTACGAACTGGCAAAGACGCTGCTGCCGAACGGCGCCGGCTCGATCATCTCGTT
CGGCATCAAGGGCGGCCGTGCCGCCGGGCGCAAGTTCATCGAGGCGCTGCGGCTGACCAGCCATCTCGCCAATGTCGGCG
ACGCCAAGACGCTGGTGATCCATCCGGCATCGACCACGCATCAGCAGATGAGCGCCGAGCAGCTCGAGGCCGCCGGCATC
GGTGAAGAGCTGATCCGGCTGTCGGTCGGCATCGAAACCGCCGACGACATTATCGCTGACCTGGCGCAGGCGCTGCGCGT
TTCGCAGAAGGGCTGA

Upstream 100 bases:

>100_bases
CAATCCAGAAGCGCCGAGCACTGAGCCTGATTGCTTCGTCGCTCCGCTCCTCGCAATGACGGAGCCTAACTAGCAATCCA
GGAAACATCCCGAGCACATC

Downstream 100 bases:

>100_bases
GCCATGCAGATCAAGGTCAACGGGACAGACACCTTCGTCGCCACCGGCGGCAAGCCGTTCGACGCGTCGCTGCCGGCCGC
AGTGTTCATTCACGGCGCCG

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAAPKPPGFETLSLHAGQHPDPLTGSRAVPIYQTTSYVFQDTDHAAALFNMERPGHLYTRISNPTIAVLEERIAALENGV
GAVATASGMAALHLAIATLLNAGDHIVASSSLYGGTINLLAHTLPRFGITTTFVAPRDHAGLAAAIQPNTRLVIGETIGN
PGLEVLDIPKVAAIAHEAKIPLLIDNTFATPYLSKPIELGADIVMHSVTKWLGGHGIAIGGVLVDGGRFDWRGSGKFPTL
TEPYAGYHDVVFDEQFGPPAFVIRARMEGLRDFGACLSPTNAFQLLQGIETLPVRMDRHVANTKAVLDFLQTNKAVDWVL
HPTLDNHPDYELAKTLLPNGAGSIISFGIKGGRAAGRKFIEALRLTSHLANVGDAKTLVIHPASTTHQQMSAEQLEAAGI
GEELIRLSVGIETADDIIADLAQALRVSQKG

Sequences:

>Translated_431_residues
MAAPKPPGFETLSLHAGQHPDPLTGSRAVPIYQTTSYVFQDTDHAAALFNMERPGHLYTRISNPTIAVLEERIAALENGV
GAVATASGMAALHLAIATLLNAGDHIVASSSLYGGTINLLAHTLPRFGITTTFVAPRDHAGLAAAIQPNTRLVIGETIGN
PGLEVLDIPKVAAIAHEAKIPLLIDNTFATPYLSKPIELGADIVMHSVTKWLGGHGIAIGGVLVDGGRFDWRGSGKFPTL
TEPYAGYHDVVFDEQFGPPAFVIRARMEGLRDFGACLSPTNAFQLLQGIETLPVRMDRHVANTKAVLDFLQTNKAVDWVL
HPTLDNHPDYELAKTLLPNGAGSIISFGIKGGRAAGRKFIEALRLTSHLANVGDAKTLVIHPASTTHQQMSAEQLEAAGI
GEELIRLSVGIETADDIIADLAQALRVSQKG
>Mature_430_residues
AAPKPPGFETLSLHAGQHPDPLTGSRAVPIYQTTSYVFQDTDHAAALFNMERPGHLYTRISNPTIAVLEERIAALENGVG
AVATASGMAALHLAIATLLNAGDHIVASSSLYGGTINLLAHTLPRFGITTTFVAPRDHAGLAAAIQPNTRLVIGETIGNP
GLEVLDIPKVAAIAHEAKIPLLIDNTFATPYLSKPIELGADIVMHSVTKWLGGHGIAIGGVLVDGGRFDWRGSGKFPTLT
EPYAGYHDVVFDEQFGPPAFVIRARMEGLRDFGACLSPTNAFQLLQGIETLPVRMDRHVANTKAVLDFLQTNKAVDWVLH
PTLDNHPDYELAKTLLPNGAGSIISFGIKGGRAAGRKFIEALRLTSHLANVGDAKTLVIHPASTTHQQMSAEQLEAAGIG
EELIRLSVGIETADDIIADLAQALRVSQKG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=430, Percent_Identity=34.1860465116279, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=31.4420803782506, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI25453487, Length=429, Percent_Identity=29.6037296037296, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI1790375, Length=421, Percent_Identity=32.7790973871734, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1789383, Length=424, Percent_Identity=25.9433962264151, Blast_Score=118, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71980708, Length=429, Percent_Identity=32.1678321678322, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI17538055, Length=426, Percent_Identity=34.9765258215962, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=426, Percent_Identity=33.8028169014084, Blast_Score=202, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=46.6666666666667, Blast_Score=353, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6319307, Length=436, Percent_Identity=33.256880733945, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321170, Length=353, Percent_Identity=28.0453257790368, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321254, Length=447, Percent_Identity=26.8456375838926, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=428, Percent_Identity=26.1682242990654, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922554, Length=430, Percent_Identity=33.4883720930233, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45806; Mature: 45675

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPKPPGFETLSLHAGQHPDPLTGSRAVPIYQTTSYVFQDTDHAAALFNMERPGHLYTR
CCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCHHEEECCCCCEEEEECCCCCCEEEE
ISNPTIAVLEERIAALENGVGAVATASGMAALHLAIATLLNAGDHIVASSSLYGGTINLL
ECCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
AHTLPRFGITTTFVAPRDHAGLAAAIQPNTRLVIGETIGNPGLEVLDIPKVAAIAHEAKI
HHHHCCCCCEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCCEEEECCHHHHHHHHCCC
PLLIDNTFATPYLSKPIELGADIVMHSVTKWLGGHGIAIGGVLVDGGRFDWRGSGKFPTL
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEECCCCCCCCCC
TEPYAGYHDVVFDEQFGPPAFVIRARMEGLRDFGACLSPTNAFQLLQGIETLPVRMDRHV
CCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHCCHHHHHHH
ANTKAVLDFLQTNKAVDWVLHPTLDNHPDYELAKTLLPNGAGSIISFGIKGGRAAGRKFI
HHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
EALRLTSHLANVGDAKTLVIHPASTTHQQMSAEQLEAAGIGEELIRLSVGIETADDIIAD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHH
LAQALRVSQKG
HHHHHHHCCCC
>Mature Secondary Structure 
AAPKPPGFETLSLHAGQHPDPLTGSRAVPIYQTTSYVFQDTDHAAALFNMERPGHLYTR
CCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCHHEEECCCCCEEEEECCCCCCEEEE
ISNPTIAVLEERIAALENGVGAVATASGMAALHLAIATLLNAGDHIVASSSLYGGTINLL
ECCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
AHTLPRFGITTTFVAPRDHAGLAAAIQPNTRLVIGETIGNPGLEVLDIPKVAAIAHEAKI
HHHHCCCCCEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCCEEEECCHHHHHHHHCCC
PLLIDNTFATPYLSKPIELGADIVMHSVTKWLGGHGIAIGGVLVDGGRFDWRGSGKFPTL
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEECCCCCCCCCC
TEPYAGYHDVVFDEQFGPPAFVIRARMEGLRDFGACLSPTNAFQLLQGIETLPVRMDRHV
CCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHCCHHHHHHH
ANTKAVLDFLQTNKAVDWVLHPTLDNHPDYELAKTLLPNGAGSIISFGIKGGRAAGRKFI
HHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
EALRLTSHLANVGDAKTLVIHPASTTHQQMSAEQLEAAGIGEELIRLSVGIETADDIIAD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHH
LAQALRVSQKG
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]