Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 192289880

GI number: 192289880

Start: 1551990

End: 1553183

Strand: Reverse

Name: carA [H]

Synonym: Rpal_1470

Alternate gene names: 192289880

Gene position: 1553183-1551990 (Counterclockwise)

Preceding gene: 192289884

Following gene: 192289879

Centisome position: 27.04

GC content: 65.49

Gene sequence:

>1194_bases
ATGACCAATTCAGCATCCCATCCCGCTTGGCCGGACCACAAGCCGACCGCGCTGCTCGTGCTTGCCGATGGCACCGTGTT
CGAAGGCTTCGGCCTCGGCGCAGAGGGCCACGCCGTTGGCGAGGTCTGCTTCAACACCGCGATGACCGGCTACGAGGAGA
TCCTCACCGATCCGTCCTACGCCGGGCAGCTCATCACCTTCACCTTCCCGCATATCGGCAACGTCGGCACCAACGACGAG
GATATCGAGACGGTGAACATGGCGGCGACGCCGGGCGCGCGGGGCGTGATCCTGCGCAGCGCCATCACCGACCCGTCGAA
CTATCGCTCGTCGCGCCATCTCGACGCCTGGCTGAAGGCTCGCGGCATCATCGGCCTGTCGGGCATCGACACCCGTGCGC
TGACCGCGCTGATCCGCAGCAAGGGCATGCCCAACGCGGTGATCGCGCATTCGCCCACCGGCGAGTTCGACCTGCACGCG
CTGAAGGAAGAGGCCCGCGAGTGGCCCGGTCTGGAAGGCATGGACCTGGTGCCGATGGTCACCTCGGCCCAGCGCTTCAC
CTGGGACGAGACGCCTTGGGCGTGGGGCGAGAGCTTCGGACGGCAGGACAAGCCCGAGTTCAACGTCGTCGCGATCGACT
ACGGCATCAAACGCAACATCCTGCGGCTGCTGGCCGGCGAAGGCTGCAAGGTCACTGTGGTGCCCGCCACCACTTCGGCC
GAAGACATCCTGGCGATGAAGCCGGACGGCGTGTTCCTGTCGAACGGCCCGGGCGATCCGGCCGCGACCGGCAAATACGC
GGTGCCGGTGATTCGGAAGGTGATCGAGACTGGCGTGCCGACCTTCGGCATCTGCCTCGGACACCAGATGCTCGGCCTCG
CGCTCGGCGGCAAGACCGTGAAGATGCATCAGGGTCACCACGGCGCCAATCATCCGGTCAAGGATCTCACCACCGGCAAG
GTCGAGATCACCTCAATGAACCACGGCTTCGCGGTCGACAAATCGACGCTGCCGGCCAATGTGCAGCAGACCCACATCTC
GCTGTTCGATGACAGCAACTGCGGCATCGCGCTGGCCGACAAACCGGTGTTCTCGGTGCAGTACCACCCCGAGGCATCGC
CCGGTCCGCAGGACTCGCACTATCTGTTCCGCCGCTTCACCGATCTGATGCGCGCGAAGAAAGCCGCCGCGTAG

Upstream 100 bases:

>100_bases
TAGGACAGCCAAATTCCCTGTCAAATCTCGTCCAAAAGCGAACAGTTGGAGGGGTTCGGCGCTTTGACGTCGGCAACGCC
CGCGACTATGTAGTGCGCTC

Downstream 100 bases:

>100_bases
TCGCGGCGGCCTTACTCATATCATCTTCTCAACTTGTCATTCCGGGGCGCGAGCGTAGCTCGCAAACCCGGAATCTGATT
TGGGCACAGTCACGTCTGGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTNSASHPAWPDHKPTALLVLADGTVFEGFGLGAEGHAVGEVCFNTAMTGYEEILTDPSYAGQLITFTFPHIGNVGTNDE
DIETVNMAATPGARGVILRSAITDPSNYRSSRHLDAWLKARGIIGLSGIDTRALTALIRSKGMPNAVIAHSPTGEFDLHA
LKEEAREWPGLEGMDLVPMVTSAQRFTWDETPWAWGESFGRQDKPEFNVVAIDYGIKRNILRLLAGEGCKVTVVPATTSA
EDILAMKPDGVFLSNGPGDPAATGKYAVPVIRKVIETGVPTFGICLGHQMLGLALGGKTVKMHQGHHGANHPVKDLTTGK
VEITSMNHGFAVDKSTLPANVQQTHISLFDDSNCGIALADKPVFSVQYHPEASPGPQDSHYLFRRFTDLMRAKKAAA

Sequences:

>Translated_397_residues
MTNSASHPAWPDHKPTALLVLADGTVFEGFGLGAEGHAVGEVCFNTAMTGYEEILTDPSYAGQLITFTFPHIGNVGTNDE
DIETVNMAATPGARGVILRSAITDPSNYRSSRHLDAWLKARGIIGLSGIDTRALTALIRSKGMPNAVIAHSPTGEFDLHA
LKEEAREWPGLEGMDLVPMVTSAQRFTWDETPWAWGESFGRQDKPEFNVVAIDYGIKRNILRLLAGEGCKVTVVPATTSA
EDILAMKPDGVFLSNGPGDPAATGKYAVPVIRKVIETGVPTFGICLGHQMLGLALGGKTVKMHQGHHGANHPVKDLTTGK
VEITSMNHGFAVDKSTLPANVQQTHISLFDDSNCGIALADKPVFSVQYHPEASPGPQDSHYLFRRFTDLMRAKKAAA
>Mature_396_residues
TNSASHPAWPDHKPTALLVLADGTVFEGFGLGAEGHAVGEVCFNTAMTGYEEILTDPSYAGQLITFTFPHIGNVGTNDED
IETVNMAATPGARGVILRSAITDPSNYRSSRHLDAWLKARGIIGLSGIDTRALTALIRSKGMPNAVIAHSPTGEFDLHAL
KEEAREWPGLEGMDLVPMVTSAQRFTWDETPWAWGESFGRQDKPEFNVVAIDYGIKRNILRLLAGEGCKVTVVPATTSAE
DILAMKPDGVFLSNGPGDPAATGKYAVPVIRKVIETGVPTFGICLGHQMLGLALGGKTVKMHQGHHGANHPVKDLTTGKV
EITSMNHGFAVDKSTLPANVQQTHISLFDDSNCGIALADKPVFSVQYHPEASPGPQDSHYLFRRFTDLMRAKKAAA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=385, Percent_Identity=37.1428571428571, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI169790915, Length=413, Percent_Identity=32.6876513317191, Blast_Score=213, Evalue=4e-55,
Organism=Homo sapiens, GI21361331, Length=413, Percent_Identity=32.6876513317191, Blast_Score=212, Evalue=5e-55,
Organism=Escherichia coli, GI1786215, Length=387, Percent_Identity=52.7131782945737, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI193204318, Length=390, Percent_Identity=38.2051282051282, Blast_Score=221, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6324878, Length=384, Percent_Identity=35.6770833333333, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6322331, Length=393, Percent_Identity=33.8422391857506, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI45555749, Length=390, Percent_Identity=36.4102564102564, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24642586, Length=390, Percent_Identity=36.4102564102564, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42617; Mature: 42486

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSASHPAWPDHKPTALLVLADGTVFEGFGLGAEGHAVGEVCFNTAMTGYEEILTDPSY
CCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AGQLITFTFPHIGNVGTNDEDIETVNMAATPGARGVILRSAITDPSNYRSSRHLDAWLKA
CCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCHHCCCCHHHHHHHHH
RGIIGLSGIDTRALTALIRSKGMPNAVIAHSPTGEFDLHALKEEAREWPGLEGMDLVPMV
CCEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
TSAQRFTWDETPWAWGESFGRQDKPEFNVVAIDYGIKRNILRLLAGEGCKVTVVPATTSA
HCCHHEECCCCCCHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCC
EDILAMKPDGVFLSNGPGDPAATGKYAVPVIRKVIETGVPTFGICLGHQMLGLALGGKTV
CCEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCEE
KMHQGHHGANHPVKDLTTGKVEITSMNHGFAVDKSTLPANVQQTHISLFDDSNCGIALAD
EEECCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCEEEEEEEEECCCCCEEEEC
KPVFSVQYHPEASPGPQDSHYLFRRFTDLMRAKKAAA
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNSASHPAWPDHKPTALLVLADGTVFEGFGLGAEGHAVGEVCFNTAMTGYEEILTDPSY
CCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AGQLITFTFPHIGNVGTNDEDIETVNMAATPGARGVILRSAITDPSNYRSSRHLDAWLKA
CCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCHHCCCCHHHHHHHHH
RGIIGLSGIDTRALTALIRSKGMPNAVIAHSPTGEFDLHALKEEAREWPGLEGMDLVPMV
CCEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
TSAQRFTWDETPWAWGESFGRQDKPEFNVVAIDYGIKRNILRLLAGEGCKVTVVPATTSA
HCCHHEECCCCCCHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCC
EDILAMKPDGVFLSNGPGDPAATGKYAVPVIRKVIETGVPTFGICLGHQMLGLALGGKTV
CCEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCEE
KMHQGHHGANHPVKDLTTGKVEITSMNHGFAVDKSTLPANVQQTHISLFDDSNCGIALAD
EEECCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCEEEEEEEEECCCCCEEEEC
KPVFSVQYHPEASPGPQDSHYLFRRFTDLMRAKKAAA
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]