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The map label for this gene is hisS

Identifier: 192289779

GI number: 192289779

Start: 1440384

End: 1441913

Strand: Reverse

Name: hisS

Synonym: Rpal_1369

Alternate gene names: 192289779

Gene position: 1441913-1440384 (Counterclockwise)

Preceding gene: 192289783

Following gene: 192289778

Centisome position: 25.1

GC content: 62.68

Gene sequence:

>1530_bases
ATGGCTGAGAAACCGAAAAAACCGCAGAAACTGCGCGCCCGGCTGCCGCGGGGCCTCGCCGATCGTGGCCCTGCCGAAAT
CGCGGCGACGCGCGCGATGGTGGAGAAGATCCGCGAGGTGTACGAACGCTACGGCTTCGAGCCGGTGGAGACCCCGGCGT
TCGAATACACTGACGCGCTCGGCAAGTTCCTGCCCGATCAGGACCGTCCCAACGAGGGCGTGTTCTCGCTGCAGGACGAC
GACGAGCAGTGGATCAGCCTGCGCTACGATCTCACCGCACCGCTGGCGCGCTACGTCGCCGAGAACTTCGATCAGCTCCC
GAAGCCATATCGCAGCTATCGGTTCGGCTGGGTATTCCGCAACGAGAAGCCCGGCCCCGGCCGCTTCCGGCAGTTCATGC
AGTTCGACGCCGACACCGTCGGCTCCGGCTCGCCCGCCGCCGACGCCGAAATGTGCATGATGGCCGCCGACACCATGGAA
GCGCTCGGCATCCCGCGCGGCAGCTATCTCGTAAAGCTGAATAATCGAAAGATTCTGGACGGCGTTCTCGAAGCGATTGG
CATTGGTGGCGATGAACACATTAAGCAGAGGCTAGTCGTCCTCCGTGCGATCGACAAATTGGATCGCTTGGGCCTGCAAG
GTGTGGAACAGCTTCTTGGTGAAGGGCGGAAGGACGAGAGCGGCGACTTCACAAGAGGAGCCTCGTTAAACGCTGGCCAG
ATAAGGGACGTCATCACCCTGCTAAACTTCGCTGGTTGGGGGGACATCGTAGACGGTTCGAATACGCACACTCTCGATGA
ATGGGAGGGGTTGCGCTTTTCAGTGAACTCAACCTTCTCTGCAGGCATTCAGGATCTACGCCAGATTACGAAGATCACAG
AGGCCTCGGGCTACGACACTGGCCGAATTCGAGTTGATAACACTGTCGTCCGCGGCCTTGAGTACTACACCGGGCCCGTA
TTCGAGGTCGAACTGCTGCTCGATACCAAGGATGAGAAGGGCCGCCCGGTGCGGTTCGGCTCGGTCGGCGGCGGCGGGCG
CTATGACGGCCTGGTGTCGCGCTTCCGCGGCGAGCCGGTGCCGGCGACCGGGTTCTCGATCGGCGTGTCGCGGCTGCAGG
CGGCGCTGACGCTGATCGGCCAGCTCGGCAACAAGCCGCAGGCCGGCCCCGTCGTCGTCACCGTGTTCGGCGGCGAGATC
GCGGGCTACCAGAAGATGGTCGCCACCTTGCGCAAAGCCGGCATCCGGGCGGAATTGTACTTGGGCAATCCCAAGCACTC
GCTCGGCCAGCAGATGAAATACGCCGACAAGCGCAACTCGCCCTGCGCCATCATCCAGGGCTCGGACGAGAAGCAGCAGG
GCATCGTGCAGATCAAGGACCTGATCCTGGGCGCCGAACTGGCGTCGCTGGAGAAGGACCGCGACGAGTATTTGAAGAAG
CAGGCCGAAGCGCAGTTCTCCTGCAAGGAAGATGAAATGGTCGCCAAGGTGCAGGAGCTGCTGCAGCGCCGCGGGGTGGC
GTGGGGATAG

Upstream 100 bases:

>100_bases
TGGACCCCGACGGCGAGGGATTTGCGCTGGATCACACAACCCAACCTTGCTGCGGCGGACACCGGCTGGTACCAACCCGG
CCCTGAGTTGAAAGGCGATC

Downstream 100 bases:

>100_bases
TCGTCATTCCGGGGCGCCGTCAGGCGCGAACCCGGAAATCCCGCAGTTCAGGGCACCATGAGAGTCCGGGTTCGCTCACG
GCGTGAGCGCCCCGGAATGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MAEKPKKPQKLRARLPRGLADRGPAEIAATRAMVEKIREVYERYGFEPVETPAFEYTDALGKFLPDQDRPNEGVFSLQDD
DEQWISLRYDLTAPLARYVAENFDQLPKPYRSYRFGWVFRNEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTME
ALGIPRGSYLVKLNNRKILDGVLEAIGIGGDEHIKQRLVVLRAIDKLDRLGLQGVEQLLGEGRKDESGDFTRGASLNAGQ
IRDVITLLNFAGWGDIVDGSNTHTLDEWEGLRFSVNSTFSAGIQDLRQITKITEASGYDTGRIRVDNTVVRGLEYYTGPV
FEVELLLDTKDEKGRPVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLQAALTLIGQLGNKPQAGPVVVTVFGGEI
AGYQKMVATLRKAGIRAELYLGNPKHSLGQQMKYADKRNSPCAIIQGSDEKQQGIVQIKDLILGAELASLEKDRDEYLKK
QAEAQFSCKEDEMVAKVQELLQRRGVAWG

Sequences:

>Translated_509_residues
MAEKPKKPQKLRARLPRGLADRGPAEIAATRAMVEKIREVYERYGFEPVETPAFEYTDALGKFLPDQDRPNEGVFSLQDD
DEQWISLRYDLTAPLARYVAENFDQLPKPYRSYRFGWVFRNEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTME
ALGIPRGSYLVKLNNRKILDGVLEAIGIGGDEHIKQRLVVLRAIDKLDRLGLQGVEQLLGEGRKDESGDFTRGASLNAGQ
IRDVITLLNFAGWGDIVDGSNTHTLDEWEGLRFSVNSTFSAGIQDLRQITKITEASGYDTGRIRVDNTVVRGLEYYTGPV
FEVELLLDTKDEKGRPVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLQAALTLIGQLGNKPQAGPVVVTVFGGEI
AGYQKMVATLRKAGIRAELYLGNPKHSLGQQMKYADKRNSPCAIIQGSDEKQQGIVQIKDLILGAELASLEKDRDEYLKK
QAEAQFSCKEDEMVAKVQELLQRRGVAWG
>Mature_508_residues
AEKPKKPQKLRARLPRGLADRGPAEIAATRAMVEKIREVYERYGFEPVETPAFEYTDALGKFLPDQDRPNEGVFSLQDDD
EQWISLRYDLTAPLARYVAENFDQLPKPYRSYRFGWVFRNEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTMEA
LGIPRGSYLVKLNNRKILDGVLEAIGIGGDEHIKQRLVVLRAIDKLDRLGLQGVEQLLGEGRKDESGDFTRGASLNAGQI
RDVITLLNFAGWGDIVDGSNTHTLDEWEGLRFSVNSTFSAGIQDLRQITKITEASGYDTGRIRVDNTVVRGLEYYTGPVF
EVELLLDTKDEKGRPVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLQAALTLIGQLGNKPQAGPVVVTVFGGEIA
GYQKMVATLRKAGIRAELYLGNPKHSLGQQMKYADKRNSPCAIIQGSDEKQQGIVQIKDLILGAELASLEKDRDEYLKKQ
AEAQFSCKEDEMVAKVQELLQRRGVAWG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=491, Percent_Identity=31.1608961303462, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI15029520, Length=470, Percent_Identity=29.7872340425532, Blast_Score=178, Evalue=1e-44,
Organism=Escherichia coli, GI1788861, Length=481, Percent_Identity=25.7796257796258, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71993686, Length=479, Percent_Identity=30.4801670146138, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI71993693, Length=479, Percent_Identity=30.4801670146138, Blast_Score=194, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6325290, Length=507, Percent_Identity=30.9664694280079, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24643061, Length=483, Percent_Identity=30.8488612836439, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24643059, Length=502, Percent_Identity=29.4820717131474, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_RHOPT (B3QII5)

Other databases:

- EMBL:   CP001096
- RefSeq:   YP_001990384.1
- ProteinModelPortal:   B3QII5
- SMR:   B3QII5
- GeneID:   6409026
- GenomeReviews:   CP001096_GR
- KEGG:   rpt:Rpal_1369
- HOGENOM:   HBG616575
- OMA:   IGKVFRD
- ProtClustDB:   PRK00037
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 56334; Mature: 56203

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKPKKPQKLRARLPRGLADRGPAEIAATRAMVEKIREVYERYGFEPVETPAFEYTDAL
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GKFLPDQDRPNEGVFSLQDDDEQWISLRYDLTAPLARYVAENFDQLPKPYRSYRFGWVFR
HHHCCCCCCCCCCEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHCCEEEEEEE
NEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTMEALGIPRGSYLVKLNNRKILD
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
GVLEAIGIGGDEHIKQRLVVLRAIDKLDRLGLQGVEQLLGEGRKDESGDFTRGASLNAGQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
IRDVITLLNFAGWGDIVDGSNTHTLDEWEGLRFSVNSTFSAGIQDLRQITKITEASGYDT
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCC
GRIRVDNTVVRGLEYYTGPVFEVELLLDTKDEKGRPVRFGSVGGGGRYDGLVSRFRGEPV
CEEEECHHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHCCCCC
PATGFSIGVSRLQAALTLIGQLGNKPQAGPVVVTVFGGEIAGYQKMVATLRKAGIRAELY
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEE
LGNPKHSLGQQMKYADKRNSPCAIIQGSDEKQQGIVQIKDLILGAELASLEKDRDEYLKK
ECCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAEAQFSCKEDEMVAKVQELLQRRGVAWG
HHHCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AEKPKKPQKLRARLPRGLADRGPAEIAATRAMVEKIREVYERYGFEPVETPAFEYTDAL
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GKFLPDQDRPNEGVFSLQDDDEQWISLRYDLTAPLARYVAENFDQLPKPYRSYRFGWVFR
HHHCCCCCCCCCCEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHCCEEEEEEE
NEKPGPGRFRQFMQFDADTVGSGSPAADAEMCMMAADTMEALGIPRGSYLVKLNNRKILD
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
GVLEAIGIGGDEHIKQRLVVLRAIDKLDRLGLQGVEQLLGEGRKDESGDFTRGASLNAGQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
IRDVITLLNFAGWGDIVDGSNTHTLDEWEGLRFSVNSTFSAGIQDLRQITKITEASGYDT
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCC
GRIRVDNTVVRGLEYYTGPVFEVELLLDTKDEKGRPVRFGSVGGGGRYDGLVSRFRGEPV
CEEEECHHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHCCCCC
PATGFSIGVSRLQAALTLIGQLGNKPQAGPVVVTVFGGEIAGYQKMVATLRKAGIRAELY
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEE
LGNPKHSLGQQMKYADKRNSPCAIIQGSDEKQQGIVQIKDLILGAELASLEKDRDEYLKK
ECCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAEAQFSCKEDEMVAKVQELLQRRGVAWG
HHHCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA